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Protein

SUMO-conjugating enzyme ubc9

Gene

hus5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin-like protein SUMO/Smt3 to other proteins. Required for efficient recovery from DNA damage or S-phase arrest and normal mitosis. This may be as part of a checkpoint independent recovery process.2 Publications

Catalytic activityi

ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB-KW
  • protein sumoylation Source: PomBase

Keywordsi

Molecular functionTransferase
Biological processDNA damage, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-3065678 SUMO is transferred from E1 to E2 (UBE2I, UBC9)
R-SPO-3108214 SUMOylation of DNA damage response and repair proteins
R-SPO-3899300 SUMOylation of transcription cofactors
R-SPO-4551638 SUMOylation of chromatin organization proteins
R-SPO-4570464 SUMOylation of RNA binding proteins
R-SPO-4615885 SUMOylation of DNA replication proteins
UniPathwayiUPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzyme ubc9 (EC:2.3.2.-)
Alternative name(s):
Ubiquitin carrier protein 9
Ubiquitin carrier protein hus5
Ubiquitin-conjugating enzyme E2-18 kDa
Gene namesi
Name:hus5
Synonyms:ubc9
ORF Names:SPAC30D11.13
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC30D11.13
PomBaseiSPAC30D11.13 hus5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825681 – 157SUMO-conjugating enzyme ubc9Add BLAST157

Proteomic databases

MaxQBiP40984
PaxDbiP40984
PRIDEiP40984

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rad60Q9USX32EBI-966468,EBI-3650521

Protein-protein interaction databases

BioGridi278671, 12 interactors
DIPiDIP-35488N
IntActiP40984, 4 interactors
STRINGi4896.SPAC30D11.13.1

Structurei

Secondary structure

1157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Beta strandi25 – 30Combined sources6
Beta strandi34 – 46Combined sources13
Turni52 – 55Combined sources4
Beta strandi57 – 63Combined sources7
Turni66 – 70Combined sources5
Beta strandi74 – 79Combined sources6
Beta strandi90 – 92Combined sources3
Helixi95 – 97Combined sources3
Turni99 – 102Combined sources4
Helixi109 – 120Combined sources12
Helixi131 – 139Combined sources9
Helixi141 – 154Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RCZX-ray1.90B2-157[»]
ProteinModelPortaliP40984
SMRiP40984
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233454
InParanoidiP40984
KOiK10577
OMAiKCKFTPP
OrthoDBiEOG092C50OL
PhylomeDBiP40984

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR027230 Ubc9
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR43927 PTHR43927, 1 hit
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P40984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLCKTRLQ EERKQWRRDH PFGFYAKPCK SSDGGLDLMN WKVGIPGKPK
60 70 80 90 100
TSWEGGLYKL TMAFPEEYPT RPPKCRFTPP LFHPNVYPSG TVCLSILNEE
110 120 130 140 150
EGWKPAITIK QILLGIQDLL DDPNIASPAQ TEAYTMFKKD KVEYEKRVRA

QARENAP
Length:157
Mass (Da):17,989
Last modified:February 1, 1995 - v1
Checksum:i4BF3A6389203D27F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81846 Genomic DNA Translation: CAA57438.1
CU329670 Genomic DNA Translation: CAA91899.1
PIRiS62571
RefSeqiNP_593204.1, NM_001018600.2

Genome annotation databases

EnsemblFungiiSPAC30D11.13.1; SPAC30D11.13.1:pep; SPAC30D11.13
GeneIDi2542196
KEGGispo:SPAC30D11.13

Similar proteinsi

Entry informationi

Entry nameiUBC9_SCHPO
AccessioniPrimary (citable) accession number: P40984
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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