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P40977

- PLC1_SCHPO

UniProt

P40977 - PLC1_SCHPO

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

plc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei456 – 4561PROSITE-ProRule annotation
Active sitei500 – 5001PROSITE-ProRule annotation
Binding sitei582 – 5821SubstrateBy similarity
Binding sitei584 – 5841SubstrateBy similarity
Binding sitei666 – 6661SubstrateBy similarity
Binding sitei693 – 6931SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi343 – 35412PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular lipid catabolic process Source: PomBase
  2. cellular response to UV Source: PomBase
  3. inositol lipid-mediated signaling Source: PomBase
  4. regulation of phosphate metabolic process Source: PomBase
  5. signal transduction Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_188291. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
Alternative name(s):
Phospholipase C-1
Short name:
PLC-1
Gene namesi
Name:plc1
ORF Names:SPAC22F8.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC22F8.11.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8998991-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1PRO_0000088513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei83 – 831Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40977.

Interactioni

Protein-protein interaction databases

BioGridi277944. 10 interactions.
MINTiMINT-4689902.
STRINGi4896.SPAC22F8.11-1.

Structurei

3D structure databases

ProteinModelPortaliP40977.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 36536EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini441 – 584144PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini638 – 753116PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini769 – 86294C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
InParanoidiP40977.
KOiK05857.
OMAiYIRALSH.
OrthoDBiEOG7NPG2M.
PhylomeDBiP40977.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00036. EF-hand_1. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40977 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNCEMVTSPE SVNLGDSNRA VSPFCLPDCS ENAVAQTRSK TLDNAALDLP
60 70 80 90 100
YVGNRKKSEQ DFFKMLSSRD RDAHSTLRKR SNSLSSFLST KSTSASENKF
110 120 130 140 150
HGGLNWLSLK LNLLLRLQGR MNSARTNTSM NPYSCDSNEN LSTLSSVNQN
160 170 180 190 200
FNSRQLLATI VPESIQNGCS LLRITKKKVR QRKVSLDPIS GYLMLDKNTG
210 220 230 240 250
KAYKKLCVDD IKEIRQGRDA RNYREQYKIS SENEKRWFTI IYCADNKLKA
260 270 280 290 300
MHMISPTLDA HNQWIMALEG LKTYRLNEFI IGLNLVCHQG EKMIDYSENL
310 320 330 340 350
NPWEKLEKEQ SAQLDLGDVH RMCQMLHLNA SMEFLEETFQ KADADHSGKL
360 370 380 390 400
SFEEFQHFVS LLKTRSEIVD IFKEYTSGSD KMSLEQFRHF LSTSQKARLD
410 420 430 440 450
SDSIRTLYVS FCSNDDSKMG LIEFTSFLLS PHNSPVVPVI QDMSRPLNEY
460 470 480 490 500
LISSSHNTYL LGKQFGGESS IEGYIRSLQR GCKCIEIDCW DGPNGPVVCH
510 520 530 540 550
GHTFTSMIKF NDVIDAIRKY AFVVSPYPLF ISLEIHCCPD QQRQMVSYMK
560 570 580 590 600
QAFGDTLVMK PVTANESVLP SPEDLLNKIL LKVKCSATPL HQFSTDILKV
610 620 630 640 650
GITDSSTDTT ESSELENSEL TGLRKGKRRM KNIIVQELQQ LAPYARSLKF
660 670 680 690 700
RNFSLPESKT YSHIFSFSER TIKKHGKAMV PRLSKHNLRY LCRVYPGPLR
710 720 730 740 750
VGSTNFNPQV YWRLGVQMVA LNWQTYDTGL QINDALFIAD PPTGYLLKPP
760 770 780 790 800
CQRIIGTTVG EEGLPRKIKL TIDVISGQQL RRARELSNSE TLSPYVEIQV
810 820 830 840 850
HSMEESPFRW CSKVVHENGF RPFWGETMVY ESIISDDFYS MIRFLVHHRG
860 870 880 890
SNGNDSIFAN FTCPIDRLQQ GYRHIRLLDM QGENLLFSSL FLKIKKEDI
Length:899
Mass (Da):102,673
Last modified:February 1, 1995 - v1
Checksum:iC7CC935DAB820245
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti480 – 4801R → A in CAA58591. (PubMed:8590474)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1.
X83615 Genomic DNA. Translation: CAA58591.1.
CU329670 Genomic DNA. Translation: CAB52721.1.
PIRiS55075.
S57259. S51092.
RefSeqiNP_594734.1. NM_001020162.2.

Genome annotation databases

EnsemblFungiiSPAC22F8.11.1; SPAC22F8.11.1:pep; SPAC22F8.11.
GeneIDi2541439.
KEGGispo:SPAC22F8.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1 .
X83615 Genomic DNA. Translation: CAA58591.1 .
CU329670 Genomic DNA. Translation: CAB52721.1 .
PIRi S55075.
S57259. S51092.
RefSeqi NP_594734.1. NM_001020162.2.

3D structure databases

ProteinModelPortali P40977.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277944. 10 interactions.
MINTi MINT-4689902.
STRINGi 4896.SPAC22F8.11-1.

Proteomic databases

MaxQBi P40977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC22F8.11.1 ; SPAC22F8.11.1:pep ; SPAC22F8.11 .
GeneIDi 2541439.
KEGGi spo:SPAC22F8.11.

Organism-specific databases

PomBasei SPAC22F8.11.

Phylogenomic databases

eggNOGi NOG149692.
InParanoidi P40977.
KOi K05857.
OMAi YIRALSH.
OrthoDBi EOG7NPG2M.
PhylomeDBi P40977.

Enzyme and pathway databases

Reactomei REACT_188291. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 20802541.
PROi P40977.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF00036. EF-hand_1. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the plc1+ gene of Schizosaccharomyces pombe, which encodes a putative phosphoinositide-specific phospholipase C."
    Andoh T., Yoko-O T., Matsui Y., Toh-e A.
    Yeast 11:179-185(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Growth of a mutant defective in a putative phosphoinositide-specific phospholipase C of Schizosaccharomyces pombe is restored by low concentrations of phosphate and inositol."
    Fankhauser H., Schweingruber A.-M., Edenharter E., Schweingruber M.E.
    Curr. Genet. 28:199-203(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPLC1_SCHPO
AccessioniPrimary (citable) accession number: P40977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3