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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

plc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei456PROSITE-ProRule annotation1
Active sitei500PROSITE-ProRule annotation1
Binding sitei582SubstrateBy similarity1
Binding sitei584SubstrateBy similarity1
Binding sitei666SubstrateBy similarity1
Binding sitei693SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi343 – 354PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-114604. GPVI-mediated activation cascade.
R-SPO-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SPO-202433. Generation of second messenger molecules.
R-SPO-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
Alternative name(s):
Phospholipase C-1
Short name:
PLC-1
Gene namesi
Name:plc1
ORF Names:SPAC22F8.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC22F8.11.
PomBaseiSPAC22F8.11. plc1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885131 – 8991-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1Add BLAST899

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40977.
PRIDEiP40977.

PTM databases

iPTMnetiP40977.

Interactioni

Protein-protein interaction databases

BioGridi277944. 10 interactors.
MINTiMINT-4689902.

Structurei

3D structure databases

ProteinModelPortaliP40977.
SMRiP40977.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini330 – 365EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini441 – 584PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST144
Domaini638 – 753PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST116
Domaini769 – 862C2PROSITE-ProRule annotationAdd BLAST94

Phylogenomic databases

InParanoidiP40977.
KOiK05857.
OMAiNNINICV.
OrthoDBiEOG092C0GR7.
PhylomeDBiP40977.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P40977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCEMVTSPE SVNLGDSNRA VSPFCLPDCS ENAVAQTRSK TLDNAALDLP
60 70 80 90 100
YVGNRKKSEQ DFFKMLSSRD RDAHSTLRKR SNSLSSFLST KSTSASENKF
110 120 130 140 150
HGGLNWLSLK LNLLLRLQGR MNSARTNTSM NPYSCDSNEN LSTLSSVNQN
160 170 180 190 200
FNSRQLLATI VPESIQNGCS LLRITKKKVR QRKVSLDPIS GYLMLDKNTG
210 220 230 240 250
KAYKKLCVDD IKEIRQGRDA RNYREQYKIS SENEKRWFTI IYCADNKLKA
260 270 280 290 300
MHMISPTLDA HNQWIMALEG LKTYRLNEFI IGLNLVCHQG EKMIDYSENL
310 320 330 340 350
NPWEKLEKEQ SAQLDLGDVH RMCQMLHLNA SMEFLEETFQ KADADHSGKL
360 370 380 390 400
SFEEFQHFVS LLKTRSEIVD IFKEYTSGSD KMSLEQFRHF LSTSQKARLD
410 420 430 440 450
SDSIRTLYVS FCSNDDSKMG LIEFTSFLLS PHNSPVVPVI QDMSRPLNEY
460 470 480 490 500
LISSSHNTYL LGKQFGGESS IEGYIRSLQR GCKCIEIDCW DGPNGPVVCH
510 520 530 540 550
GHTFTSMIKF NDVIDAIRKY AFVVSPYPLF ISLEIHCCPD QQRQMVSYMK
560 570 580 590 600
QAFGDTLVMK PVTANESVLP SPEDLLNKIL LKVKCSATPL HQFSTDILKV
610 620 630 640 650
GITDSSTDTT ESSELENSEL TGLRKGKRRM KNIIVQELQQ LAPYARSLKF
660 670 680 690 700
RNFSLPESKT YSHIFSFSER TIKKHGKAMV PRLSKHNLRY LCRVYPGPLR
710 720 730 740 750
VGSTNFNPQV YWRLGVQMVA LNWQTYDTGL QINDALFIAD PPTGYLLKPP
760 770 780 790 800
CQRIIGTTVG EEGLPRKIKL TIDVISGQQL RRARELSNSE TLSPYVEIQV
810 820 830 840 850
HSMEESPFRW CSKVVHENGF RPFWGETMVY ESIISDDFYS MIRFLVHHRG
860 870 880 890
SNGNDSIFAN FTCPIDRLQQ GYRHIRLLDM QGENLLFSSL FLKIKKEDI
Length:899
Mass (Da):102,673
Last modified:February 1, 1995 - v1
Checksum:iC7CC935DAB820245
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti480R → A in CAA58591 (PubMed:8590474).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1.
X83615 Genomic DNA. Translation: CAA58591.1.
CU329670 Genomic DNA. Translation: CAB52721.1.
PIRiS55075.
S57259. S51092.
RefSeqiNP_594734.1. NM_001020162.2.

Genome annotation databases

EnsemblFungiiSPAC22F8.11.1; SPAC22F8.11.1:pep; SPAC22F8.11.
GeneIDi2541439.
KEGGispo:SPAC22F8.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1.
X83615 Genomic DNA. Translation: CAA58591.1.
CU329670 Genomic DNA. Translation: CAB52721.1.
PIRiS55075.
S57259. S51092.
RefSeqiNP_594734.1. NM_001020162.2.

3D structure databases

ProteinModelPortaliP40977.
SMRiP40977.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277944. 10 interactors.
MINTiMINT-4689902.

PTM databases

iPTMnetiP40977.

Proteomic databases

MaxQBiP40977.
PRIDEiP40977.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC22F8.11.1; SPAC22F8.11.1:pep; SPAC22F8.11.
GeneIDi2541439.
KEGGispo:SPAC22F8.11.

Organism-specific databases

EuPathDBiFungiDB:SPAC22F8.11.
PomBaseiSPAC22F8.11. plc1.

Phylogenomic databases

InParanoidiP40977.
KOiK05857.
OMAiNNINICV.
OrthoDBiEOG092C0GR7.
PhylomeDBiP40977.

Enzyme and pathway databases

ReactomeiR-SPO-114604. GPVI-mediated activation cascade.
R-SPO-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SPO-202433. Generation of second messenger molecules.
R-SPO-5607764. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

PROiPR:P40977.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLC1_SCHPO
AccessioniPrimary (citable) accession number: P40977
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 10, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.