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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

plc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei456 – 4561PROSITE-ProRule annotation
Active sitei500 – 5001PROSITE-ProRule annotation
Binding sitei582 – 5821SubstrateBy similarity
Binding sitei584 – 5841SubstrateBy similarity
Binding sitei666 – 6661SubstrateBy similarity
Binding sitei693 – 6931SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi343 – 35412PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_271870. Role of second messengers in netrin-1 signaling.
REACT_279829. Role of phospholipids in phagocytosis.
REACT_298532. VEGFR2 mediated cell proliferation.
REACT_298801. PLC beta mediated events.
REACT_307194. Synthesis of IP3 and IP4 in the cytosol.
REACT_311821. DAG and IP3 signaling.
REACT_320766. Generation of second messenger molecules.
REACT_337563. GPVI-mediated activation cascade.
REACT_362437. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
Alternative name(s):
Phospholipase C-1
Short name:
PLC-1
Gene namesi
Name:plc1
ORF Names:SPAC22F8.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC22F8.11.
PomBaseiSPAC22F8.11. plc1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8998991-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1PRO_0000088513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei83 – 831Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40977.

Interactioni

Protein-protein interaction databases

BioGridi277944. 10 interactions.
MINTiMINT-4689902.
STRINGi4896.SPAC22F8.11.1.

Structurei

3D structure databases

ProteinModelPortaliP40977.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 36536EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini441 – 584144PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini638 – 753116PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini769 – 86294C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
InParanoidiP40977.
KOiK05857.
OMAiDDKMSYE.
OrthoDBiEOG7NPG2M.
PhylomeDBiP40977.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00036. EF-hand_1. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCEMVTSPE SVNLGDSNRA VSPFCLPDCS ENAVAQTRSK TLDNAALDLP
60 70 80 90 100
YVGNRKKSEQ DFFKMLSSRD RDAHSTLRKR SNSLSSFLST KSTSASENKF
110 120 130 140 150
HGGLNWLSLK LNLLLRLQGR MNSARTNTSM NPYSCDSNEN LSTLSSVNQN
160 170 180 190 200
FNSRQLLATI VPESIQNGCS LLRITKKKVR QRKVSLDPIS GYLMLDKNTG
210 220 230 240 250
KAYKKLCVDD IKEIRQGRDA RNYREQYKIS SENEKRWFTI IYCADNKLKA
260 270 280 290 300
MHMISPTLDA HNQWIMALEG LKTYRLNEFI IGLNLVCHQG EKMIDYSENL
310 320 330 340 350
NPWEKLEKEQ SAQLDLGDVH RMCQMLHLNA SMEFLEETFQ KADADHSGKL
360 370 380 390 400
SFEEFQHFVS LLKTRSEIVD IFKEYTSGSD KMSLEQFRHF LSTSQKARLD
410 420 430 440 450
SDSIRTLYVS FCSNDDSKMG LIEFTSFLLS PHNSPVVPVI QDMSRPLNEY
460 470 480 490 500
LISSSHNTYL LGKQFGGESS IEGYIRSLQR GCKCIEIDCW DGPNGPVVCH
510 520 530 540 550
GHTFTSMIKF NDVIDAIRKY AFVVSPYPLF ISLEIHCCPD QQRQMVSYMK
560 570 580 590 600
QAFGDTLVMK PVTANESVLP SPEDLLNKIL LKVKCSATPL HQFSTDILKV
610 620 630 640 650
GITDSSTDTT ESSELENSEL TGLRKGKRRM KNIIVQELQQ LAPYARSLKF
660 670 680 690 700
RNFSLPESKT YSHIFSFSER TIKKHGKAMV PRLSKHNLRY LCRVYPGPLR
710 720 730 740 750
VGSTNFNPQV YWRLGVQMVA LNWQTYDTGL QINDALFIAD PPTGYLLKPP
760 770 780 790 800
CQRIIGTTVG EEGLPRKIKL TIDVISGQQL RRARELSNSE TLSPYVEIQV
810 820 830 840 850
HSMEESPFRW CSKVVHENGF RPFWGETMVY ESIISDDFYS MIRFLVHHRG
860 870 880 890
SNGNDSIFAN FTCPIDRLQQ GYRHIRLLDM QGENLLFSSL FLKIKKEDI
Length:899
Mass (Da):102,673
Last modified:February 1, 1995 - v1
Checksum:iC7CC935DAB820245
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti480 – 4801R → A in CAA58591 (PubMed:8590474).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1.
X83615 Genomic DNA. Translation: CAA58591.1.
CU329670 Genomic DNA. Translation: CAB52721.1.
PIRiS55075.
S57259. S51092.
RefSeqiNP_594734.1. NM_001020162.2.

Genome annotation databases

EnsemblFungiiSPAC22F8.11.1; SPAC22F8.11.1:pep; SPAC22F8.11.
GeneIDi2541439.
KEGGispo:SPAC22F8.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1.
X83615 Genomic DNA. Translation: CAA58591.1.
CU329670 Genomic DNA. Translation: CAB52721.1.
PIRiS55075.
S57259. S51092.
RefSeqiNP_594734.1. NM_001020162.2.

3D structure databases

ProteinModelPortaliP40977.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277944. 10 interactions.
MINTiMINT-4689902.
STRINGi4896.SPAC22F8.11.1.

Proteomic databases

MaxQBiP40977.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC22F8.11.1; SPAC22F8.11.1:pep; SPAC22F8.11.
GeneIDi2541439.
KEGGispo:SPAC22F8.11.

Organism-specific databases

EuPathDBiFungiDB:SPAC22F8.11.
PomBaseiSPAC22F8.11. plc1.

Phylogenomic databases

eggNOGiNOG149692.
InParanoidiP40977.
KOiK05857.
OMAiDDKMSYE.
OrthoDBiEOG7NPG2M.
PhylomeDBiP40977.

Enzyme and pathway databases

ReactomeiREACT_271870. Role of second messengers in netrin-1 signaling.
REACT_279829. Role of phospholipids in phagocytosis.
REACT_298532. VEGFR2 mediated cell proliferation.
REACT_298801. PLC beta mediated events.
REACT_307194. Synthesis of IP3 and IP4 in the cytosol.
REACT_311821. DAG and IP3 signaling.
REACT_320766. Generation of second messenger molecules.
REACT_337563. GPVI-mediated activation cascade.
REACT_362437. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

NextBioi20802541.
PROiP40977.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00036. EF-hand_1. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the plc1+ gene of Schizosaccharomyces pombe, which encodes a putative phosphoinositide-specific phospholipase C."
    Andoh T., Yoko-O T., Matsui Y., Toh-e A.
    Yeast 11:179-185(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Growth of a mutant defective in a putative phosphoinositide-specific phospholipase C of Schizosaccharomyces pombe is restored by low concentrations of phosphate and inositol."
    Fankhauser H., Schweingruber A.-M., Edenharter E., Schweingruber M.E.
    Curr. Genet. 28:199-203(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPLC1_SCHPO
AccessioniPrimary (citable) accession number: P40977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.