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P40977

- PLC1_SCHPO

UniProt

P40977 - PLC1_SCHPO

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

plc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei456 – 4561PROSITE-ProRule annotation
    Active sitei500 – 5001PROSITE-ProRule annotation
    Binding sitei582 – 5821SubstrateBy similarity
    Binding sitei584 – 5841SubstrateBy similarity
    Binding sitei666 – 6661SubstrateBy similarity
    Binding sitei693 – 6931SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi343 – 35412PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. protein binding Source: PomBase
    4. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular lipid catabolic process Source: PomBase
    2. cellular response to UV Source: PomBase
    3. inositol lipid-mediated signaling Source: PomBase
    4. regulation of phosphate metabolic process Source: PomBase
    5. signal transduction Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_188291. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
    Alternative name(s):
    Phospholipase C-1
    Short name:
    PLC-1
    Gene namesi
    Name:plc1
    ORF Names:SPAC22F8.11
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC22F8.11.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8998991-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1PRO_0000088513Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei83 – 831Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40977.

    Interactioni

    Protein-protein interaction databases

    BioGridi277944. 10 interactions.
    MINTiMINT-4689902.
    STRINGi4896.SPAC22F8.11-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP40977.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini330 – 36536EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini441 – 584144PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini638 – 753116PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini769 – 86294C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    KOiK05857.
    OMAiYIRALSH.
    OrthoDBiEOG7NPG2M.
    PhylomeDBiP40977.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF00036. EF-hand_1. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40977-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNCEMVTSPE SVNLGDSNRA VSPFCLPDCS ENAVAQTRSK TLDNAALDLP    50
    YVGNRKKSEQ DFFKMLSSRD RDAHSTLRKR SNSLSSFLST KSTSASENKF 100
    HGGLNWLSLK LNLLLRLQGR MNSARTNTSM NPYSCDSNEN LSTLSSVNQN 150
    FNSRQLLATI VPESIQNGCS LLRITKKKVR QRKVSLDPIS GYLMLDKNTG 200
    KAYKKLCVDD IKEIRQGRDA RNYREQYKIS SENEKRWFTI IYCADNKLKA 250
    MHMISPTLDA HNQWIMALEG LKTYRLNEFI IGLNLVCHQG EKMIDYSENL 300
    NPWEKLEKEQ SAQLDLGDVH RMCQMLHLNA SMEFLEETFQ KADADHSGKL 350
    SFEEFQHFVS LLKTRSEIVD IFKEYTSGSD KMSLEQFRHF LSTSQKARLD 400
    SDSIRTLYVS FCSNDDSKMG LIEFTSFLLS PHNSPVVPVI QDMSRPLNEY 450
    LISSSHNTYL LGKQFGGESS IEGYIRSLQR GCKCIEIDCW DGPNGPVVCH 500
    GHTFTSMIKF NDVIDAIRKY AFVVSPYPLF ISLEIHCCPD QQRQMVSYMK 550
    QAFGDTLVMK PVTANESVLP SPEDLLNKIL LKVKCSATPL HQFSTDILKV 600
    GITDSSTDTT ESSELENSEL TGLRKGKRRM KNIIVQELQQ LAPYARSLKF 650
    RNFSLPESKT YSHIFSFSER TIKKHGKAMV PRLSKHNLRY LCRVYPGPLR 700
    VGSTNFNPQV YWRLGVQMVA LNWQTYDTGL QINDALFIAD PPTGYLLKPP 750
    CQRIIGTTVG EEGLPRKIKL TIDVISGQQL RRARELSNSE TLSPYVEIQV 800
    HSMEESPFRW CSKVVHENGF RPFWGETMVY ESIISDDFYS MIRFLVHHRG 850
    SNGNDSIFAN FTCPIDRLQQ GYRHIRLLDM QGENLLFSSL FLKIKKEDI 899
    Length:899
    Mass (Da):102,673
    Last modified:February 1, 1995 - v1
    Checksum:iC7CC935DAB820245
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti480 – 4801R → A in CAA58591. (PubMed:8590474)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38309 Genomic DNA. Translation: BAA07426.1.
    X83615 Genomic DNA. Translation: CAA58591.1.
    CU329670 Genomic DNA. Translation: CAB52721.1.
    PIRiS55075.
    S57259. S51092.
    RefSeqiNP_594734.1. NM_001020162.2.

    Genome annotation databases

    EnsemblFungiiSPAC22F8.11.1; SPAC22F8.11.1:pep; SPAC22F8.11.
    GeneIDi2541439.
    KEGGispo:SPAC22F8.11.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38309 Genomic DNA. Translation: BAA07426.1 .
    X83615 Genomic DNA. Translation: CAA58591.1 .
    CU329670 Genomic DNA. Translation: CAB52721.1 .
    PIRi S55075.
    S57259. S51092.
    RefSeqi NP_594734.1. NM_001020162.2.

    3D structure databases

    ProteinModelPortali P40977.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277944. 10 interactions.
    MINTi MINT-4689902.
    STRINGi 4896.SPAC22F8.11-1.

    Proteomic databases

    MaxQBi P40977.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC22F8.11.1 ; SPAC22F8.11.1:pep ; SPAC22F8.11 .
    GeneIDi 2541439.
    KEGGi spo:SPAC22F8.11.

    Organism-specific databases

    PomBasei SPAC22F8.11.

    Phylogenomic databases

    eggNOGi NOG149692.
    KOi K05857.
    OMAi YIRALSH.
    OrthoDBi EOG7NPG2M.
    PhylomeDBi P40977.

    Enzyme and pathway databases

    Reactomei REACT_188291. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 20802541.
    PROi P40977.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF00036. EF-hand_1. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the plc1+ gene of Schizosaccharomyces pombe, which encodes a putative phosphoinositide-specific phospholipase C."
      Andoh T., Yoko-O T., Matsui Y., Toh-e A.
      Yeast 11:179-185(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Growth of a mutant defective in a putative phosphoinositide-specific phospholipase C of Schizosaccharomyces pombe is restored by low concentrations of phosphate and inositol."
      Fankhauser H., Schweingruber A.-M., Edenharter E., Schweingruber M.E.
      Curr. Genet. 28:199-203(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPLC1_SCHPO
    AccessioniPrimary (citable) accession number: P40977
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3