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P40977

- PLC1_SCHPO

UniProt

P40977 - PLC1_SCHPO

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene
plc1, SPAC22F8.11
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei456 – 4561 By similarity
Active sitei500 – 5001 By similarity
Binding sitei582 – 5821Substrate By similarity
Binding sitei584 – 5841Substrate By similarity
Binding sitei666 – 6661Substrate By similarity
Binding sitei693 – 6931Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi343 – 35412 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. protein binding Source: PomBase
  4. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular lipid catabolic process Source: PomBase
  2. cellular response to UV Source: PomBase
  3. inositol lipid-mediated signaling Source: PomBase
  4. regulation of phosphate metabolic process Source: PomBase
  5. signal transduction Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_188291. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
Alternative name(s):
Phospholipase C-1
Short name:
PLC-1
Gene namesi
Name:plc1
ORF Names:SPAC22F8.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC22F8.11.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8998991-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1PRO_0000088513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei83 – 831Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40977.

Interactioni

Protein-protein interaction databases

BioGridi277944. 10 interactions.
MINTiMINT-4689902.
STRINGi4896.SPAC22F8.11-1.

Structurei

3D structure databases

ProteinModelPortaliP40977.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 36536EF-handAdd
BLAST
Domaini441 – 584144PI-PLC X-boxAdd
BLAST
Domaini638 – 753116PI-PLC Y-boxAdd
BLAST
Domaini769 – 86294C2Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 EF-hand domain.

Phylogenomic databases

eggNOGiNOG149692.
KOiK05857.
OMAiYIRALSH.
OrthoDBiEOG7NPG2M.
PhylomeDBiP40977.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00036. EF-hand_1. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40977-1 [UniParc]FASTAAdd to Basket

« Hide

MNCEMVTSPE SVNLGDSNRA VSPFCLPDCS ENAVAQTRSK TLDNAALDLP    50
YVGNRKKSEQ DFFKMLSSRD RDAHSTLRKR SNSLSSFLST KSTSASENKF 100
HGGLNWLSLK LNLLLRLQGR MNSARTNTSM NPYSCDSNEN LSTLSSVNQN 150
FNSRQLLATI VPESIQNGCS LLRITKKKVR QRKVSLDPIS GYLMLDKNTG 200
KAYKKLCVDD IKEIRQGRDA RNYREQYKIS SENEKRWFTI IYCADNKLKA 250
MHMISPTLDA HNQWIMALEG LKTYRLNEFI IGLNLVCHQG EKMIDYSENL 300
NPWEKLEKEQ SAQLDLGDVH RMCQMLHLNA SMEFLEETFQ KADADHSGKL 350
SFEEFQHFVS LLKTRSEIVD IFKEYTSGSD KMSLEQFRHF LSTSQKARLD 400
SDSIRTLYVS FCSNDDSKMG LIEFTSFLLS PHNSPVVPVI QDMSRPLNEY 450
LISSSHNTYL LGKQFGGESS IEGYIRSLQR GCKCIEIDCW DGPNGPVVCH 500
GHTFTSMIKF NDVIDAIRKY AFVVSPYPLF ISLEIHCCPD QQRQMVSYMK 550
QAFGDTLVMK PVTANESVLP SPEDLLNKIL LKVKCSATPL HQFSTDILKV 600
GITDSSTDTT ESSELENSEL TGLRKGKRRM KNIIVQELQQ LAPYARSLKF 650
RNFSLPESKT YSHIFSFSER TIKKHGKAMV PRLSKHNLRY LCRVYPGPLR 700
VGSTNFNPQV YWRLGVQMVA LNWQTYDTGL QINDALFIAD PPTGYLLKPP 750
CQRIIGTTVG EEGLPRKIKL TIDVISGQQL RRARELSNSE TLSPYVEIQV 800
HSMEESPFRW CSKVVHENGF RPFWGETMVY ESIISDDFYS MIRFLVHHRG 850
SNGNDSIFAN FTCPIDRLQQ GYRHIRLLDM QGENLLFSSL FLKIKKEDI 899
Length:899
Mass (Da):102,673
Last modified:February 1, 1995 - v1
Checksum:iC7CC935DAB820245
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti480 – 4801R → A in CAA58591. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1.
X83615 Genomic DNA. Translation: CAA58591.1.
CU329670 Genomic DNA. Translation: CAB52721.1.
PIRiS55075.
S57259. S51092.
RefSeqiNP_594734.1. NM_001020162.2.

Genome annotation databases

EnsemblFungiiSPAC22F8.11.1; SPAC22F8.11.1:pep; SPAC22F8.11.
GeneIDi2541439.
KEGGispo:SPAC22F8.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38309 Genomic DNA. Translation: BAA07426.1 .
X83615 Genomic DNA. Translation: CAA58591.1 .
CU329670 Genomic DNA. Translation: CAB52721.1 .
PIRi S55075.
S57259. S51092.
RefSeqi NP_594734.1. NM_001020162.2.

3D structure databases

ProteinModelPortali P40977.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277944. 10 interactions.
MINTi MINT-4689902.
STRINGi 4896.SPAC22F8.11-1.

Proteomic databases

MaxQBi P40977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC22F8.11.1 ; SPAC22F8.11.1:pep ; SPAC22F8.11 .
GeneIDi 2541439.
KEGGi spo:SPAC22F8.11.

Organism-specific databases

PomBasei SPAC22F8.11.

Phylogenomic databases

eggNOGi NOG149692.
KOi K05857.
OMAi YIRALSH.
OrthoDBi EOG7NPG2M.
PhylomeDBi P40977.

Enzyme and pathway databases

Reactomei REACT_188291. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 20802541.
PROi P40977.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF00036. EF-hand_1. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the plc1+ gene of Schizosaccharomyces pombe, which encodes a putative phosphoinositide-specific phospholipase C."
    Andoh T., Yoko-O T., Matsui Y., Toh-e A.
    Yeast 11:179-185(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Growth of a mutant defective in a putative phosphoinositide-specific phospholipase C of Schizosaccharomyces pombe is restored by low concentrations of phosphate and inositol."
    Fankhauser H., Schweingruber A.-M., Edenharter E., Schweingruber M.E.
    Curr. Genet. 28:199-203(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPLC1_SCHPO
AccessioniPrimary (citable) accession number: P40977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi