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P40976 (LYS2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-aminoadipate-semialdehyde dehydrogenase

EC=1.2.1.31
Alternative name(s):
Alpha-aminoadipate reductase
Short name=Alpha-AR
Gene names
Name:lys1
ORF Names:SPAP7G5.04c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length1419 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH.

Catalytic activity

(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.

Cofactor

Binds 1 phosphopantetheine covalently Potential.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.

Subcellular location

Cytoplasm Ref.4.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Contains 1 acyl carrier domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14191419L-aminoadipate-semialdehyde dehydrogenase
PRO_0000193152

Regions

Domain885 – 95369Acyl carrier

Amino acid modifications

Modified residue9161O-(pantetheine 4'-phosphoryl)serine Potential

Experimental info

Mutagenesis9131G → A: No activity. Ref.3
Mutagenesis9161S → A or T: No activity. Ref.3
Sequence conflict90 – 912MT → IA in AAC15909. Ref.1
Sequence conflict1961Missing in AAC15909. Ref.1
Sequence conflict4871D → DG in AAC15909. Ref.1
Sequence conflict5001R → G in AAC15909. Ref.1
Sequence conflict600 – 6023Missing in AAC15909. Ref.1
Sequence conflict620 – 6212AR → GP in AAC15909. Ref.1
Sequence conflict7121Y → S in AAC15909. Ref.1
Sequence conflict926 – 9283LRK → PSQ in AAC15909. Ref.1
Sequence conflict1203 – 12053VVV → AAA in AAC15909. Ref.1
Sequence conflict1225 – 12284LVRM → WSK in AAC15909. Ref.1
Sequence conflict12391P → A in AAC15909. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40976 [UniParc].

Last modified February 21, 2001. Version 3.
Checksum: 09AFBEFE09F21A0C

FASTA1,419156,894
        10         20         30         40         50         60 
MSQTAPSDTE YNQRLERWSE RLKSQTISHL PTDYSRPVPS RLVEAVFERT LPEDAKTALI 

        70         80         90        100        110        120 
KVYVAAQAKG ILVTPFNILL TIFIILVSRM TGDEDISIGT SSENAIPFVL RTFIQPSDSF 

       130        140        150        160        170        180 
LDLLAKVCDL EKEGSSDAVD FSDLINFLNA KLSKKDDPRK TLVHLRFYNA PDAPSENFLS 

       190        200        210        220        230        240 
TTGLDVDLTV LVSVKKPSDQ LTSLRSQFTF PDLQLKLIYN QLLFSESRVN IVADQLLKLV 

       250        260        270        280        290        300 
VSASKDVTGP IGALDLMTPT QMNVLPDPTV DLDWSGYRGA IQDIFASNAA KFPDRECIVV 

       310        320        330        340        350        360 
TPSVTIDAPV TSYTYRQIDE SSNILAHHLV KNGIERGDVV MVYAYRGVDL VVAVMGVLKA 

       370        380        390        400        410        420 
GATFSVIDPA YPPARQIIYL SVAKPRALVV LEDAGVLSPT VVEYVEKSLE LKTYVPALKL 

       430        440        450        460        470        480 
AKDGSLTGGS VSKGADDILQ HVLHLKSEQT GVVVGPDSTP TLSFTSGSEG IPKGVKGRHF 

       490        500        510        520        530        540 
SLAYYFDWMA QEFNLSESDR FTMLSGIAHD PIQRDIFTPL FLGASLIVPT AEDIGTPGQL 

       550        560        570        580        590        600 
AQWANKYKVT VTHLTPAMGQ LLAAQADEPI PSLHHAFFVG DILTKRDCLR LQVLANNVNV 

       610        620        630        640        650        660 
VNMYGTTETQ RSVSYFVVPA RSQDQTFLES QKDVIPAGRG MKNVQLLVIN RFDTNKICGI 

       670        680        690        700        710        720 
GEVGEIYLRA GGLAEGYLGN DELTSKKFLK SWFADPSKFV DRTPENAPWK PYWFGIRDRM 

       730        740        750        760        770        780 
YRSGDLGRYL PTGNVECSGR ADDQIKIRGF RIELGEINTH LSRHPNVREN ITLVRRDKDE 

       790        800        810        820        830        840 
EPTLVAYIVP QGLNKDDFDS ATESEDIVVN GLKKYRKLIH DIREYLKTKL PSYAIPSVIV 

       850        860        870        880        890        900 
PLHKMPLNPN GKIDKPALPF PDTSQLAAAS RSHSKHGVDE TLTATERDIR DIWLRIIPHA 

       910        920        930        940        950        960 
TDVNKKASFF DIGGHSILAT RLIFELRKKF AVNVPLGLVF SEPTIEGLAK EIERMKSGEM 

       970        980        990       1000       1010       1020 
ISVMDIGKEE TREPEIEYGK DALDLVDLIP KEFPTSKDLG IDEPKTVFLT GANGYLGVFI 

      1030       1040       1050       1060       1070       1080 
LRDLMTRSSN LKVIALVRAS SEEHGLKRLK DSCTAYGVWD ESWAQKISVV NGDLALENWG 

      1090       1100       1110       1120       1130       1140 
IEERKWNKLT EVVDYVIHNG ALVHWVYPYS KLRGPNVMGT ITALKLCSLG KGKSLSFVSS 

      1150       1160       1170       1180       1190       1200 
TSTVDTEYYV NLSNEITSKG GNGIPESDPL QGSSKDLHTG YGQSKWVSEY LVRQAGLRGL 

      1210       1220       1230       1240       1250       1260 
RGVVVRPGYI LGDSKSGAIN TDDFLVRMVK GCIELGLYPN INNTVNMVPA DHVARVVTAS 

      1270       1280       1290       1300       1310       1320 
AFHPEQGVIV AHVTSHPRLR FNQFLGTLST FGFNTKLSEY VNWRIALERF VINESHDSAL 

      1330       1340       1350       1360       1370       1380 
YPLLHFVLDN LPANTKAPEL DDTNTREILK RDASWTNVDV SNGAAILEHE MGLYLSYLVA 

      1390       1400       1410 
IGFLPKPTLE GKKLPEVKIN EATLEKLASA GGRGGAPTH 

« Hide

References

« Hide 'large scale' references
[1]"Molecular properties of the lys1+ gene and the regulation of alpha-aminoadipate reductase in Schizosaccharomyces pombe."
Ford R.A., Bhattacharjee J.K.
Curr. Genet. 28:131-137(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Posttranslational activation, site-directed mutation and phylogenetic analyses of the lysine biosynthesis enzymes alpha-aminoadipate reductase Lys1p (AAR) and the phosphopantetheinyl transferase Lys7p (PPTase) from Schizosaccharomyces pombe."
Guo S., Bhattacharjee J.K.
Yeast 21:1279-1288(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF GLY-913 AND SER-916.
Strain: 972 / ATCC 24843.
[4]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15923 Genomic DNA. Translation: AAC15909.1.
CU329670 Genomic DNA. Translation: CAB88271.1.
PIRS57264.
RefSeqNP_594314.1. NM_001019737.2.

3D structure databases

ProteinModelPortalP40976.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid278556. 3 interactions.
MINTMINT-4689880.
STRING4896.SPAP7G5.04c-1.

Proteomic databases

MaxQBP40976.
PaxDbP40976.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAP7G5.04c.1; SPAP7G5.04c.1:pep; SPAP7G5.04c.
GeneID2542079.
KEGGspo:SPAP7G5.04c.

Organism-specific databases

PomBaseSPAP7G5.04c.

Phylogenomic databases

eggNOGCOG3320.
HOGENOMHOG000191209.
KOK00143.
OMADRFTMLS.
OrthoDBEOG74TX6Z.
PhylomeDBP40976.

Enzyme and pathway databases

UniPathwayUPA00033; UER00032.

Family and domain databases

Gene3D1.10.1200.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR014397. L-NH2adipate-semiAld_DH_lsu.
IPR013120. Male_sterile_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006162. PPantetheine_attach_site.
IPR010080. Thioester_reductase-like_dom.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
PF07993. NAD_binding_4. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFPIRSF001617. Alpha-AR. 1 hit.
SUPFAMSSF47336. SSF47336. 1 hit.
TIGRFAMsTIGR01733. AA-adenyl-dom. 1 hit.
TIGR03443. alpha_am_amid. 1 hit.
TIGR01746. Thioester-redct. 1 hit.
PROSITEPS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20803152.

Entry information

Entry nameLYS2_SCHPO
AccessionPrimary (citable) accession number: P40976
Secondary accession number(s): Q9P770
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 21, 2001
Last modified: May 14, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways