Reviewed,
UniProtKB/Swiss-Prot P40976 (LYS2_SCHPO)
Last modified
June 16, 2009.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: L-aminoadipate-semialdehyde dehydrogenase EC=1.2.1.31 Alternative name(s): Alpha-aminoadipate reductase Short name=Alpha-AR | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (Fission yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4896 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 1419 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. |
| Catalytic activity | L-2-aminoadipate 6-semialdehyde + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H. |
| Cofactor | Binds 1 phosphopantetheine covalently Potential. |
| Pathway | |
| Subcellular location | |
| Sequence similarities | Belongs to the ATP-dependent AMP-binding enzyme family. Contains 1 acyl carrier domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Lysine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | NADP Phosphopantetheine |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | lysine biosynthetic process Ref.3 Inferred from direct assay. Source: GeneDB_SPombe oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytosol Ref.4 Inferred from direct assay. Source: GeneDB_SPombe |
| Molecular function | L-aminoadipate-semialdehyde dehydrogenase activity Ref.3 Inferred from direct assay. Source: GeneDB_SPombe acyl carrier activityInferred from electronic annotation. Source: InterPro acyl-CoA binding Ref.3Inferred from direct assay. Source: GeneDB_SPombe ligase activityInferred from electronic annotation. Source: InterPro phosphopantetheine bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1419 | 1419 | L-aminoadipate-semialdehyde dehydrogenase | PRO_0000193152 | |||||
Regions | |||||||||
| Domain | 885 – 953 | 69 | Acyl carrier | ||||||
Amino acid modifications | |||||||||
| Modified residue | 916 | 1 | O-(pantetheine 4'-phosphoryl)serine Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 913 | 1 | G → A: No activity. Ref.3 | ||||||
| Mutagenesis | 916 | 1 | S → A or T: No activity. Ref.3 | ||||||
| Sequence conflict | 90 – 91 | 2 | MT → IA in AAC15909. Ref.1 | ||||||
| Sequence conflict | 196 | 1 | Missing in AAC15909. Ref.1 | ||||||
| Sequence conflict | 487 | 1 | D → DG in AAC15909. Ref.1 | ||||||
| Sequence conflict | 500 | 1 | R → G in AAC15909. Ref.1 | ||||||
| Sequence conflict | 600 – 602 | 3 | Missing Ref.1 | ||||||
| Sequence conflict | 620 – 621 | 2 | AR → GP in AAC15909. Ref.1 | ||||||
| Sequence conflict | 712 | 1 | Y → S in AAC15909. Ref.1 | ||||||
| Sequence conflict | 926 – 928 | 3 | LRK → PSQ in AAC15909. Ref.1 | ||||||
| Sequence conflict | 1203 – 1205 | 3 | VVV → AAA in AAC15909. Ref.1 | ||||||
| Sequence conflict | 1225 – 1228 | 4 | LVRM → WSK in AAC15909. Ref.1 | ||||||
| Sequence conflict | 1239 | 1 | P → A in AAC15909. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular properties of the lys1+ gene and the regulation of alpha-aminoadipate reductase in Schizosaccharomyces pombe." Ford R.A., Bhattacharjee J.K. Curr. Genet. 28:131-137(1995) [PubMed: 8590464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972. |
| [2] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972. |
| [3] | "Posttranslational activation, site-directed mutation and phylogenetic analyses of the lysine biosynthesis enzymes alpha-aminoadipate reductase Lys1p (AAR) and the phosphopantetheinyl transferase Lys7p (PPTase) from Schizosaccharomyces pombe." Guo S., Bhattacharjee J.K. Yeast 21:1279-1288(2004) [PubMed: 15546125] [Abstract] Cited for: MUTAGENESIS OF GLY-913 AND SER-916. Strain: ATCC 38366 / 972. |
| [4] | "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
Cross-references
Sequence databases | |
|---|---|
| U15923 Genomic DNA. Translation: AAC15909.1. CU329670 Genomic DNA. Translation: CAB88271.1. | |
| PIR | S57264. |
| RefSeq | NP_594314.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1AMU based on UniProtKB P14687. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2542079. |
| KEGG | spo:SPAP7G5.04c. |
| NMPDR | fig|4896.1.peg.4284. |
Organism-specific databases | |
| GeneDB_Spombe | SPAP7G5.04c. |
Phylogenomic databases | |
| OMA | P40976. THLTPAM. |
Enzyme and pathway databases | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-001916-MON. |
| BRENDA | 1.2.1.31. 653. |
Gene expression databases | |
| ArrayExpress | P40976. |
Family and domain databases | |
| InterPro | IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR000873. AMP-dep_Synth/Lig. IPR014397. L-NH2adipate-semiAld_DH_lsu. IPR013120. Male_sterile_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth_bd. IPR006162. Ppantne_S. IPR010080. Thioester_reductase. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00501. AMP-binding. 1 hit. PF07993. NAD_binding_4. 1 hit. PF00550. PP-binding. 1 hit. [Graphical view] |
| PIRSF | PIRSF001617. Alpha-AR. 1 hit. |
| TIGRFAMs | TIGR01733. AA-adenyl-dom. 1 hit. TIGR03443. alpha_am_amid. 1 hit. TIGR01746. Thioester-redct. 1 hit. |
| PROSITE | PS50075. ACP_DOMAIN. 1 hit. PS00455. AMP_BINDING. 1 hit. PS00012. PHOSPHOPANTETHEINE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LYS2_SCHPO | ||||||||
| Accession | Primary (citable) accession number: P40976 Secondary accession number(s): Q9P770 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


