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Protein

L-2-aminoadipate reductase

Gene

lys1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.By similarity

Catalytic activityi

(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.By similarity
(S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate = L-2-aminoadipate + NADPH + ATP.By similarity

Cofactori

pantetheine 4'-phosphateBy similarityNote: Binds 1 phosphopantetheine covalently.By similarity

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (fungal route).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. L-2-aminoadipate reductase (lys1)
  2. Saccharopine dehydrogenase [NADP(+), L-glutamate-forming] (lys9)
  3. Saccharopine dehydrogenase [NAD(+), L-lysine-forming] (lys3)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (fungal route), the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • lysine biosynthetic process Source: PomBase
  • lysine biosynthetic process via aminoadipic acid Source: PomBase

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Lysine biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00033; UER00032

Names & Taxonomyi

Protein namesi
Recommended name:
L-2-aminoadipate reductase (EC:1.2.1.31By similarity, EC:1.2.1.95By similarity)
Alternative name(s):
Alpha-aminoadipate reductase
Short name:
Alpha-AR
L-aminoadipate-semialdehyde dehydrogenase
Gene namesi
Name:lys1
ORF Names:SPAP7G5.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAP7G5.04c
PomBaseiSPAP7G5.04c lys1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi913G → A: No activity. 1 Publication1
Mutagenesisi916S → A or T: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931521 – 1419L-2-aminoadipate reductaseAdd BLAST1419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei916O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

MaxQBiP40976
PaxDbiP40976
PRIDEiP40976

PTM databases

iPTMnetiP40976

Interactioni

Protein-protein interaction databases

BioGridi278556, 1 interactor
STRINGi4896.SPAP7G5.04c.1

Structurei

3D structure databases

ProteinModelPortaliP40976
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini880 – 956CarrierPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000191209
InParanoidiP40976
KOiK00143
OMAiMIYAYRG
OrthoDBiEOG092C07V5
PhylomeDBiP40976

Family and domain databases

CDDicd05235 SDR_e1, 1 hit
Gene3Di1.10.1200.10, 1 hit
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR001242 Condensatn
IPR014397 Lys2
IPR013120 Male_sterile_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
IPR020806 PKS_PP-bd
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR010080 Thioester_reductase-like_dom
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PF00668 Condensation, 1 hit
PF07993 NAD_binding_4, 1 hit
PF00550 PP-binding, 1 hit
SMARTiView protein in SMART
SM00823 PKS_PP, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
TIGR03443 alpha_am_amid, 1 hit
TIGR01746 Thioester-redct, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit

Sequencei

Sequence statusi: Complete.

P40976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTAPSDTE YNQRLERWSE RLKSQTISHL PTDYSRPVPS RLVEAVFERT
60 70 80 90 100
LPEDAKTALI KVYVAAQAKG ILVTPFNILL TIFIILVSRM TGDEDISIGT
110 120 130 140 150
SSENAIPFVL RTFIQPSDSF LDLLAKVCDL EKEGSSDAVD FSDLINFLNA
160 170 180 190 200
KLSKKDDPRK TLVHLRFYNA PDAPSENFLS TTGLDVDLTV LVSVKKPSDQ
210 220 230 240 250
LTSLRSQFTF PDLQLKLIYN QLLFSESRVN IVADQLLKLV VSASKDVTGP
260 270 280 290 300
IGALDLMTPT QMNVLPDPTV DLDWSGYRGA IQDIFASNAA KFPDRECIVV
310 320 330 340 350
TPSVTIDAPV TSYTYRQIDE SSNILAHHLV KNGIERGDVV MVYAYRGVDL
360 370 380 390 400
VVAVMGVLKA GATFSVIDPA YPPARQIIYL SVAKPRALVV LEDAGVLSPT
410 420 430 440 450
VVEYVEKSLE LKTYVPALKL AKDGSLTGGS VSKGADDILQ HVLHLKSEQT
460 470 480 490 500
GVVVGPDSTP TLSFTSGSEG IPKGVKGRHF SLAYYFDWMA QEFNLSESDR
510 520 530 540 550
FTMLSGIAHD PIQRDIFTPL FLGASLIVPT AEDIGTPGQL AQWANKYKVT
560 570 580 590 600
VTHLTPAMGQ LLAAQADEPI PSLHHAFFVG DILTKRDCLR LQVLANNVNV
610 620 630 640 650
VNMYGTTETQ RSVSYFVVPA RSQDQTFLES QKDVIPAGRG MKNVQLLVIN
660 670 680 690 700
RFDTNKICGI GEVGEIYLRA GGLAEGYLGN DELTSKKFLK SWFADPSKFV
710 720 730 740 750
DRTPENAPWK PYWFGIRDRM YRSGDLGRYL PTGNVECSGR ADDQIKIRGF
760 770 780 790 800
RIELGEINTH LSRHPNVREN ITLVRRDKDE EPTLVAYIVP QGLNKDDFDS
810 820 830 840 850
ATESEDIVVN GLKKYRKLIH DIREYLKTKL PSYAIPSVIV PLHKMPLNPN
860 870 880 890 900
GKIDKPALPF PDTSQLAAAS RSHSKHGVDE TLTATERDIR DIWLRIIPHA
910 920 930 940 950
TDVNKKASFF DIGGHSILAT RLIFELRKKF AVNVPLGLVF SEPTIEGLAK
960 970 980 990 1000
EIERMKSGEM ISVMDIGKEE TREPEIEYGK DALDLVDLIP KEFPTSKDLG
1010 1020 1030 1040 1050
IDEPKTVFLT GANGYLGVFI LRDLMTRSSN LKVIALVRAS SEEHGLKRLK
1060 1070 1080 1090 1100
DSCTAYGVWD ESWAQKISVV NGDLALENWG IEERKWNKLT EVVDYVIHNG
1110 1120 1130 1140 1150
ALVHWVYPYS KLRGPNVMGT ITALKLCSLG KGKSLSFVSS TSTVDTEYYV
1160 1170 1180 1190 1200
NLSNEITSKG GNGIPESDPL QGSSKDLHTG YGQSKWVSEY LVRQAGLRGL
1210 1220 1230 1240 1250
RGVVVRPGYI LGDSKSGAIN TDDFLVRMVK GCIELGLYPN INNTVNMVPA
1260 1270 1280 1290 1300
DHVARVVTAS AFHPEQGVIV AHVTSHPRLR FNQFLGTLST FGFNTKLSEY
1310 1320 1330 1340 1350
VNWRIALERF VINESHDSAL YPLLHFVLDN LPANTKAPEL DDTNTREILK
1360 1370 1380 1390 1400
RDASWTNVDV SNGAAILEHE MGLYLSYLVA IGFLPKPTLE GKKLPEVKIN
1410
EATLEKLASA GGRGGAPTH
Length:1,419
Mass (Da):156,894
Last modified:February 21, 2001 - v3
Checksum:i09AFBEFE09F21A0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90 – 91MT → IA in AAC15909 (PubMed:8590464).Curated2
Sequence conflicti196Missing in AAC15909 (PubMed:8590464).Curated1
Sequence conflicti487D → DG in AAC15909 (PubMed:8590464).Curated1
Sequence conflicti500R → G in AAC15909 (PubMed:8590464).Curated1
Sequence conflicti600 – 602Missing in AAC15909 (PubMed:8590464).Curated3
Sequence conflicti620 – 621AR → GP in AAC15909 (PubMed:8590464).Curated2
Sequence conflicti712Y → S in AAC15909 (PubMed:8590464).Curated1
Sequence conflicti926 – 928LRK → PSQ in AAC15909 (PubMed:8590464).Curated3
Sequence conflicti1203 – 1205VVV → AAA in AAC15909 (PubMed:8590464).Curated3
Sequence conflicti1225 – 1228LVRM → WSK in AAC15909 (PubMed:8590464).Curated4
Sequence conflicti1239P → A in AAC15909 (PubMed:8590464).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15923 Genomic DNA Translation: AAC15909.1
CU329670 Genomic DNA Translation: CAB88271.1
PIRiS57264
RefSeqiNP_594314.1, NM_001019737.2

Genome annotation databases

EnsemblFungiiSPAP7G5.04c.1; SPAP7G5.04c.1:pep; SPAP7G5.04c
GeneIDi2542079
KEGGispo:SPAP7G5.04c

Similar proteinsi

Entry informationi

Entry nameiLYS2_SCHPO
AccessioniPrimary (citable) accession number: P40976
Secondary accession number(s): Q9P770
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 21, 2001
Last modified: June 20, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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