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P40976

- LYS2_SCHPO

UniProt

P40976 - LYS2_SCHPO

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Protein

L-aminoadipate-semialdehyde dehydrogenase

Gene

lys1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH.

Catalytic activityi

(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.

Cofactori

pantetheine 4'-phosphateCuratedNote: Binds 1 phosphopantetheine covalently.Curated

Pathwayi

GO - Molecular functioni

  1. fatty-acyl-CoA binding Source: PomBase
  2. L-aminoadipate-semialdehyde dehydrogenase activity Source: PomBase
  3. phosphopantetheine binding Source: InterPro

GO - Biological processi

  1. lysine biosynthetic process Source: PomBase
  2. lysine biosynthetic process via aminoadipic acid Source: PomBase
  3. negative regulation of lysine biosynthetic process via aminoadipic acid Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00033; UER00032.

Names & Taxonomyi

Protein namesi
Recommended name:
L-aminoadipate-semialdehyde dehydrogenase (EC:1.2.1.31)
Alternative name(s):
Alpha-aminoadipate reductase
Short name:
Alpha-AR
Gene namesi
Name:lys1
ORF Names:SPAP7G5.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAP7G5.04c.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi913 – 9131G → A: No activity. 1 Publication
Mutagenesisi916 – 9161S → A or T: No activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14191419L-aminoadipate-semialdehyde dehydrogenasePRO_0000193152Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei916 – 9161O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

MaxQBiP40976.
PaxDbiP40976.

Interactioni

Protein-protein interaction databases

BioGridi278556. 2 interactions.
MINTiMINT-4689880.
STRINGi4896.SPAP7G5.04c-1.

Structurei

3D structure databases

ProteinModelPortaliP40976.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini885 – 95369Acyl carrierPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 acyl carrier domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3320.
HOGENOMiHOG000191209.
InParanoidiP40976.
KOiK00143.
OMAiDRFTMLS.
OrthoDBiEOG74TX6Z.
PhylomeDBiP40976.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR014397. L-NH2adipate-semiAld_DH_lsu.
IPR013120. Male_sterile_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006162. PPantetheine_attach_site.
IPR010080. Thioester_reductase-like_dom.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
PF07993. NAD_binding_4. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
PIRSFiPIRSF001617. Alpha-AR. 1 hit.
SUPFAMiSSF47336. SSF47336. 1 hit.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
TIGR03443. alpha_am_amid. 1 hit.
TIGR01746. Thioester-redct. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40976-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQTAPSDTE YNQRLERWSE RLKSQTISHL PTDYSRPVPS RLVEAVFERT
60 70 80 90 100
LPEDAKTALI KVYVAAQAKG ILVTPFNILL TIFIILVSRM TGDEDISIGT
110 120 130 140 150
SSENAIPFVL RTFIQPSDSF LDLLAKVCDL EKEGSSDAVD FSDLINFLNA
160 170 180 190 200
KLSKKDDPRK TLVHLRFYNA PDAPSENFLS TTGLDVDLTV LVSVKKPSDQ
210 220 230 240 250
LTSLRSQFTF PDLQLKLIYN QLLFSESRVN IVADQLLKLV VSASKDVTGP
260 270 280 290 300
IGALDLMTPT QMNVLPDPTV DLDWSGYRGA IQDIFASNAA KFPDRECIVV
310 320 330 340 350
TPSVTIDAPV TSYTYRQIDE SSNILAHHLV KNGIERGDVV MVYAYRGVDL
360 370 380 390 400
VVAVMGVLKA GATFSVIDPA YPPARQIIYL SVAKPRALVV LEDAGVLSPT
410 420 430 440 450
VVEYVEKSLE LKTYVPALKL AKDGSLTGGS VSKGADDILQ HVLHLKSEQT
460 470 480 490 500
GVVVGPDSTP TLSFTSGSEG IPKGVKGRHF SLAYYFDWMA QEFNLSESDR
510 520 530 540 550
FTMLSGIAHD PIQRDIFTPL FLGASLIVPT AEDIGTPGQL AQWANKYKVT
560 570 580 590 600
VTHLTPAMGQ LLAAQADEPI PSLHHAFFVG DILTKRDCLR LQVLANNVNV
610 620 630 640 650
VNMYGTTETQ RSVSYFVVPA RSQDQTFLES QKDVIPAGRG MKNVQLLVIN
660 670 680 690 700
RFDTNKICGI GEVGEIYLRA GGLAEGYLGN DELTSKKFLK SWFADPSKFV
710 720 730 740 750
DRTPENAPWK PYWFGIRDRM YRSGDLGRYL PTGNVECSGR ADDQIKIRGF
760 770 780 790 800
RIELGEINTH LSRHPNVREN ITLVRRDKDE EPTLVAYIVP QGLNKDDFDS
810 820 830 840 850
ATESEDIVVN GLKKYRKLIH DIREYLKTKL PSYAIPSVIV PLHKMPLNPN
860 870 880 890 900
GKIDKPALPF PDTSQLAAAS RSHSKHGVDE TLTATERDIR DIWLRIIPHA
910 920 930 940 950
TDVNKKASFF DIGGHSILAT RLIFELRKKF AVNVPLGLVF SEPTIEGLAK
960 970 980 990 1000
EIERMKSGEM ISVMDIGKEE TREPEIEYGK DALDLVDLIP KEFPTSKDLG
1010 1020 1030 1040 1050
IDEPKTVFLT GANGYLGVFI LRDLMTRSSN LKVIALVRAS SEEHGLKRLK
1060 1070 1080 1090 1100
DSCTAYGVWD ESWAQKISVV NGDLALENWG IEERKWNKLT EVVDYVIHNG
1110 1120 1130 1140 1150
ALVHWVYPYS KLRGPNVMGT ITALKLCSLG KGKSLSFVSS TSTVDTEYYV
1160 1170 1180 1190 1200
NLSNEITSKG GNGIPESDPL QGSSKDLHTG YGQSKWVSEY LVRQAGLRGL
1210 1220 1230 1240 1250
RGVVVRPGYI LGDSKSGAIN TDDFLVRMVK GCIELGLYPN INNTVNMVPA
1260 1270 1280 1290 1300
DHVARVVTAS AFHPEQGVIV AHVTSHPRLR FNQFLGTLST FGFNTKLSEY
1310 1320 1330 1340 1350
VNWRIALERF VINESHDSAL YPLLHFVLDN LPANTKAPEL DDTNTREILK
1360 1370 1380 1390 1400
RDASWTNVDV SNGAAILEHE MGLYLSYLVA IGFLPKPTLE GKKLPEVKIN
1410
EATLEKLASA GGRGGAPTH
Length:1,419
Mass (Da):156,894
Last modified:February 21, 2001 - v3
Checksum:i09AFBEFE09F21A0C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 912MT → IA in AAC15909. (PubMed:8590464)Curated
Sequence conflicti196 – 1961Missing in AAC15909. (PubMed:8590464)Curated
Sequence conflicti487 – 4871D → DG in AAC15909. (PubMed:8590464)Curated
Sequence conflicti500 – 5001R → G in AAC15909. (PubMed:8590464)Curated
Sequence conflicti600 – 6023Missing in AAC15909. (PubMed:8590464)Curated
Sequence conflicti620 – 6212AR → GP in AAC15909. (PubMed:8590464)Curated
Sequence conflicti712 – 7121Y → S in AAC15909. (PubMed:8590464)Curated
Sequence conflicti926 – 9283LRK → PSQ in AAC15909. (PubMed:8590464)Curated
Sequence conflicti1203 – 12053VVV → AAA in AAC15909. (PubMed:8590464)Curated
Sequence conflicti1225 – 12284LVRM → WSK in AAC15909. (PubMed:8590464)Curated
Sequence conflicti1239 – 12391P → A in AAC15909. (PubMed:8590464)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15923 Genomic DNA. Translation: AAC15909.1.
CU329670 Genomic DNA. Translation: CAB88271.1.
PIRiS57264.
RefSeqiNP_594314.1. NM_001019737.2.

Genome annotation databases

EnsemblFungiiSPAP7G5.04c.1; SPAP7G5.04c.1:pep; SPAP7G5.04c.
GeneIDi2542079.
KEGGispo:SPAP7G5.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15923 Genomic DNA. Translation: AAC15909.1 .
CU329670 Genomic DNA. Translation: CAB88271.1 .
PIRi S57264.
RefSeqi NP_594314.1. NM_001019737.2.

3D structure databases

ProteinModelPortali P40976.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 278556. 2 interactions.
MINTi MINT-4689880.
STRINGi 4896.SPAP7G5.04c-1.

Proteomic databases

MaxQBi P40976.
PaxDbi P40976.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAP7G5.04c.1 ; SPAP7G5.04c.1:pep ; SPAP7G5.04c .
GeneIDi 2542079.
KEGGi spo:SPAP7G5.04c.

Organism-specific databases

PomBasei SPAP7G5.04c.

Phylogenomic databases

eggNOGi COG3320.
HOGENOMi HOG000191209.
InParanoidi P40976.
KOi K00143.
OMAi DRFTMLS.
OrthoDBi EOG74TX6Z.
PhylomeDBi P40976.

Enzyme and pathway databases

UniPathwayi UPA00033 ; UER00032 .

Miscellaneous databases

NextBioi 20803152.

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR010071. AA_adenyl_domain.
IPR009081. Acyl_carrier_prot-like.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR014397. L-NH2adipate-semiAld_DH_lsu.
IPR013120. Male_sterile_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR006162. PPantetheine_attach_site.
IPR010080. Thioester_reductase-like_dom.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
PF07993. NAD_binding_4. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
PIRSFi PIRSF001617. Alpha-AR. 1 hit.
SUPFAMi SSF47336. SSF47336. 1 hit.
TIGRFAMsi TIGR01733. AA-adenyl-dom. 1 hit.
TIGR03443. alpha_am_amid. 1 hit.
TIGR01746. Thioester-redct. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular properties of the lys1+ gene and the regulation of alpha-aminoadipate reductase in Schizosaccharomyces pombe."
    Ford R.A., Bhattacharjee J.K.
    Curr. Genet. 28:131-137(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Posttranslational activation, site-directed mutation and phylogenetic analyses of the lysine biosynthesis enzymes alpha-aminoadipate reductase Lys1p (AAR) and the phosphopantetheinyl transferase Lys7p (PPTase) from Schizosaccharomyces pombe."
    Guo S., Bhattacharjee J.K.
    Yeast 21:1279-1288(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLY-913 AND SER-916.
    Strain: 972 / ATCC 24843.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLYS2_SCHPO
AccessioniPrimary (citable) accession number: P40976
Secondary accession number(s): Q9P770
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 21, 2001
Last modified: November 26, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3