Reviewed, UniProtKB/Swiss-Prot P40974 (PUO_MICRU)

Names and origin

Protein names

Recommended name:
    Putrescine oxidase
    EC=1.4.3.10

Gene names

Name:

puo

Organism

Micrococcus rubens (Deinococcus erythromyxa) (Kocuria rosea)

Taxonomic identifier

1275 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Putrescine + O(2) + H(2)O = 4-aminobutanal + NH(3) + H(2)O(2).
Cofactor	FAD.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro putrescine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Putrescine oxidase		PRO_0000099866
Regions
	Nucleotide binding	15 – 70	56	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P40974-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9C4567051B010582	FASTA	478	51,914
	10 20 30 40 50 60 MTDQRTLGSE TAIERDVVVV GAGPAGLMAA RTLVAAGRTV AVLEARDRVG GRTWSKTVDG 70 80 90 100 110 120 AFLEIGGQWI SPDQTELLAL VDELGLETYQ RYREGESVYL APDGTRHTYT GSMFPAGEST 130 140 150 160 170 180 IVEMEKLVAL LDGLVAEIGA TEPWAHPAAR ELDTISFHHW LRQHSDDEAA CSNIGIFVAG 190 200 210 220 230 240 GMLTKPAHAF SVLQAVLMAA SAGSFSNLVD EDFILDRRVV GGMQSVSETM AAELGEDVVF 250 260 270 280 290 300 LDTPVRTIRW AGDGGTYAEH VPGTPVTVWS DRLTVRAKDV VVAVPPNLYS RISFEPPLPR 310 320 330 340 350 360 LQHQMHQHQS LGLVIKVHAV YETPFWRDKG LSGTGFGAHE LSQEVYDNTN HGDPRGTLVG 370 380 390 400 410 420 FVSDERADEL FGLPAEERRR LILESLSHYL GEEALHPVVY YESDFGSEEW TRGAYAASYD 430 440 450 460 470 LGGLHRYGAH QRTPVGPIRW ACSDLAAEGY QHVDGALRQG RLAAAEVLGA GSLTGAER

References

[1]

"Putrescine oxidase of Micrococcus rubens: primary structure and Escherichia coli." Ishizuka H., Horinouchi S., Beppu T. J. Gen. Microbiol. 139:425-432(1993) [PubMed: 8473854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 186 / DSM 20447 / IFO 3768 / JCM 11614 / NCTC 7523.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D12511 Genomic DNA. Translation: BAA02074.1.
PIR	A47693.
3D structure databases
HSSP	HSSP built from PDB template 1GOS based on UniProtKB P27338.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-23.
Family and domain databases
InterPro	IPR000759. Adrndx_reductase. IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE. PR00757. AMINEOXDASEF.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PUO_MICRU

Accession

Primary (citable) accession number: P40974

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)