Reviewed, UniProtKB/Swiss-Prot P40973 (PEL_LILLO)

Names and origin

Protein names	Recommended name:     Pectate lyase     EC=4.2.2.2
Organism	Lilium longiflorum (Trumpet lily)
Taxonomic identifier	4690 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Liliales › Liliaceae › Lilium

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 434	412	Pectate lyase		PRO_0000024888
Sites
	Active site	312	1	Potential
	Metal binding	232	1	Calcium By similarity
	Metal binding	256	1	Calcium By similarity
	Metal binding	260	1	Calcium By similarity
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P40973-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C1F3E30AD2BBD064	FASTA	434	48,457
	10 20 30 40 50 60 MKAAQFFLYS LLFFASAALS SANIAEFDEY WQKKSKVAQA KAKKAYTPHP EEVTNHFNKA 70 80 90 100 110 120 VHSSFEGNST RRNLRTNKLG QCLATNPIDR CWRCKKNWSA NRKDLVKCVK GFGRKTTGGA 130 140 150 160 170 180 AGEIYVVTDP SDDSLTDPKF GTLRWGVIQD RPLWIIFGKS MVIRLKQELI INNDKTIDGR 190 200 210 220 230 240 GANVQIAGGA QLTVQFVHNV IIHGIHIHDI KPGEGGLIRD SEKHSGIRTR SDGDGISIIG 250 260 270 280 290 300 SSNIWIDHVS LARCSDGLID VILGSTAITI SNCHLTEHDD VMLLGASDTY TQDEIMQVTV 310 320 330 340 350 360 AFNHFGRGLV QRMPRCRYGF VHVVNNDYTH WIMYAVGGSQ HPTIISQGNR YIAPHIEAAK 370 380 390 400 410 420 EVTKRDYAEP AEWSKWTWKS QGDLFVSGAF FVESGGPFEN KYSKKDLIKA KPGTFVQRLT 430 RFSGALNCKE NMEC

References

[1]	Kim S.R., Finkel D.J., An G. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nellie white. Tissue: Pollen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z17328 mRNA. Translation: CAA78976.1. L18911 mRNA. Translation: AAA33398.1.
PIR	S29612.
3D structure databases
HSSP	HSSP built from PDB template 1JRG based on UniProtKB P29155.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.2. 1959.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR007524. Pec_lyase_N. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. PF04431. Pec_lyase_N. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL_LILLO

Accession

Primary (citable) accession number: P40973

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 22, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)