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Reviewed, UniProtKB/Swiss-Prot P40972 (PEL_TOBAC)

Last modified September 22, 2009. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pectate lyase
    EC=4.2.2.2
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

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Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion. Required for its activity By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Developmental stage

Maximally expressed late in pollen development.

Sequence similarities

Belongs to the polysaccharide lyase 1 family.

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Ontologies

Keywords
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMGlycoprotein
Gene Ontology (GO)
   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 397372Pectate lyase
PRO_0000024892

Sites

Active site2721 Potential
Metal binding1911Calcium By similarity
Metal binding2161Calcium By similarity
Metal binding2201Calcium By similarity

Amino acid modifications

Glycosylation1341N-linked (GlcNAc...) Potential
Glycosylation2271N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1561S → C in CAA47631. Ref.1
Sequence conflict189 – 1902GS → SG in CAA47631. Ref.1
Sequence conflict200 – 2023SSH → DSR in CAA47631. Ref.1
Sequence conflict2491H → N in CAA47631. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P40972-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: EF0A82CE5DA7643F

FASTA39744,352
        10         20         30         40         50         60 
MDVYRIRISV FFLLVLLTFA ALTTATNIPR RQLSNKKYKG PCRAENAIDK CWRCDPNWAE 

        70         80         90        100        110        120 
NRQKMADCAL GFGSNAIGGK LGRIYVVTDN SDDDVVDPKP GTLRYGVIQK EPLWIIFGKN 

       130        140        150        160        170        180 
MKIKLSRELI VTSNKTIDGR GFNVHIQNGA GIKIQSASNI IISNLRIHNI VPTPGGLLRE 

       190        200        210        220        230        240 
SEDHVGLRGS DEGDGISIFS SHDIWIDHIS MSRATDGLID AVAASTNITI SNCHFTDHEK 

       250        260        270        280        290        300 
VMLFGANDHY VLDKDMKITL AYNHFGKRLD QRMPRCRFGF FHLVNNDYTH WERYAIGGSS 

       310        320        330        340        350        360 
GATIISQGNR FIAEDELLVK EVTYREKLTA SVAEWMKWTW ISDGDDMENG ATFTPSGDQN 

       370        380        390 
LLDKIDHLNL IKPEPSSKVG ILTKFSGALS CVKGRPC

« Hide

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References

[1]"Isolation and characterization of a tobacco gene with homology to pectate lyase which is specifically expressed during microsporogenesis."
Rogers H.J., Harvey A., Lonsdale D.M.
Plant Mol. Biol. 20:493-502(1992) [PubMed: 1421152] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Samsun.
Tissue: Pollen.

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Cross-references

Sequence databases

X67158 Genomic DNA. Translation: CAA47630.1.
X67159 mRNA. Translation: CAA47631.1.
X61102 Genomic DNA. Translation: CAA43414.1.
PIRS26211.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyPL1. Polysaccharide Lyase Family 1.

Enzyme and pathway databases

BRENDA4.2.2.2. 298.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR018082. AmbAllergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
SM00710. PbH1. 3 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePEL_TOBAC
AccessionPrimary (citable) accession number: P40972
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 22, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents