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Protein

Centromere DNA-binding protein complex CBF3 subunit B

Gene

CEP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi14 – 4229Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • centromeric DNA binding Source: SGD
  • DNA binding, bending Source: SGD
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mitotic spindle assembly checkpoint Source: SGD
  • septin ring assembly Source: SGD
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere DNA-binding protein complex CBF3 subunit B
Short name:
Centromere protein 3
Gene namesi
Name:CEP3
Synonyms:CBF3, CBF3B, CSL1
Ordered Locus Names:YMR168C
ORF Names:YM8520.17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR168C.
SGDiS000004778. CEP3.

Subcellular locationi

GO - Cellular componenti

  • CBF3 complex Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Centromere DNA-binding protein complex CBF3 subunit BPRO_0000114941Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei575 – 5751PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40969.

PTM databases

iPTMnetiP40969.

Interactioni

Subunit structurei

Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D.

Binary interactionsi

WithEntry#Exp.IntActNotes
CTF13P352032EBI-4077,EBI-4085

Protein-protein interaction databases

BioGridi35346. 78 interactions.
DIPiDIP-5956N.
IntActiP40969. 10 interactions.
MINTiMINT-416717.

Structurei

Secondary structure

1
608
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi58 – 7114Combined sources
Turni72 – 743Combined sources
Helixi75 – 795Combined sources
Helixi85 – 873Combined sources
Turni92 – 954Combined sources
Helixi96 – 10510Combined sources
Helixi108 – 12114Combined sources
Helixi123 – 1308Combined sources
Helixi134 – 14613Combined sources
Helixi154 – 17320Combined sources
Helixi176 – 1827Combined sources
Helixi186 – 1927Combined sources
Helixi200 – 2023Combined sources
Helixi204 – 22219Combined sources
Turni223 – 2275Combined sources
Helixi231 – 24010Combined sources
Turni241 – 2444Combined sources
Helixi245 – 2484Combined sources
Helixi250 – 26516Combined sources
Helixi280 – 30223Combined sources
Helixi313 – 3153Combined sources
Helixi338 – 35619Combined sources
Turni357 – 3604Combined sources
Helixi367 – 38519Combined sources
Helixi394 – 42229Combined sources
Helixi427 – 44317Combined sources
Helixi444 – 4463Combined sources
Turni447 – 4493Combined sources
Helixi451 – 4555Combined sources
Helixi457 – 47620Combined sources
Helixi480 – 49516Combined sources
Helixi498 – 5003Combined sources
Helixi501 – 52222Combined sources
Beta strandi530 – 5345Combined sources
Helixi536 – 55015Combined sources
Helixi555 – 5628Combined sources
Helixi589 – 60012Combined sources
Helixi603 – 6064Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QUQX-ray2.80A47-608[»]
2VEQX-ray2.49A48-608[»]
ProteinModelPortaliP40969.
SMRiP40969. Positions 49-608.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40969.

Family & Domainsi

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000111345.
InParanoidiP40969.
KOiK11575.
OMAiHAAYLED.
OrthoDBiEOG092C1C0B.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR031760. Cep3_C.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF16846. Cep3. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNRTTQLKS KHPCSVCTRR KVKCDRMIPC GNCRKRGQDS ECMKSTKLIT
60 70 80 90 100
ASSSKEYLPD LLLFWQNYEY WITNIGLYKT KQRDLTRTPA NLDTDTEECM
110 120 130 140 150
FWMNYLQKDQ SFQLMNFAME NLGALYFGSI GDISELYLRV EQYWDRRADK
160 170 180 190 200
NHSVDGKYWD ALIWSVFTMC IYYMPVEKLA EIFSVYPLHE YLGSNKRLNW
210 220 230 240 250
EDGMQLVMCQ NFARCSLFQL KQCDFMAHPD IRLVQAYLIL ATTTFPYDEP
260 270 280 290 300
LLANSLLTQC IHTFKNFHVD DFRPLLNDDP VESIAKVTLG RIFYRLCGCD
310 320 330 340 350
YLQSGPRKPI ALHTEVSSLL QHAAYLQDLP NVDVYREENS TEVLYWKIIS
360 370 380 390 400
LDRDLDQYLN KSSKPPLKTL DAIRRELDIF QYKVDSLEED FRSNNSRFQK
410 420 430 440 450
FIALFQISTV SWKLFKMYLI YYDTADSLLK VIHYSKVIIS LIVNNFHAKS
460 470 480 490 500
EFFNRHPMVM QTITRVVSFI SFYQIFVESA AVKQLLVDLT ELTANLPTIF
510 520 530 540 550
GSKLDKLVYL TERLSKLKLL WDKVQLLDSG DSFYHPVFKI LQNDIKIIEL
560 570 580 590 600
KNDEMFSLIK GLGSLVPLNK LRQESLLEEE DENNTEPSDF RTIVEEFQSE

YNISDILS
Length:608
Mass (Da):71,358
Last modified:February 1, 1995 - v1
Checksum:i2E24A0508080A09B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81396 Genomic DNA. Translation: CAA57159.1.
U12339 Genomic DNA. Translation: AAA57074.1.
Z49705 Genomic DNA. Translation: CAA89804.1.
BK006946 Genomic DNA. Translation: DAA10064.1.
PIRiS51790.
RefSeqiNP_013891.1. NM_001182672.1.

Genome annotation databases

EnsemblFungiiYMR168C; YMR168C; YMR168C.
GeneIDi855204.
KEGGisce:YMR168C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81396 Genomic DNA. Translation: CAA57159.1.
U12339 Genomic DNA. Translation: AAA57074.1.
Z49705 Genomic DNA. Translation: CAA89804.1.
BK006946 Genomic DNA. Translation: DAA10064.1.
PIRiS51790.
RefSeqiNP_013891.1. NM_001182672.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QUQX-ray2.80A47-608[»]
2VEQX-ray2.49A48-608[»]
ProteinModelPortaliP40969.
SMRiP40969. Positions 49-608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35346. 78 interactions.
DIPiDIP-5956N.
IntActiP40969. 10 interactions.
MINTiMINT-416717.

PTM databases

iPTMnetiP40969.

Proteomic databases

MaxQBiP40969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR168C; YMR168C; YMR168C.
GeneIDi855204.
KEGGisce:YMR168C.

Organism-specific databases

EuPathDBiFungiDB:YMR168C.
SGDiS000004778. CEP3.

Phylogenomic databases

HOGENOMiHOG000111345.
InParanoidiP40969.
KOiK11575.
OMAiHAAYLED.
OrthoDBiEOG092C1C0B.

Enzyme and pathway databases

BioCyciYEAST:G3O-32858-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40969.
PROiP40969.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR031760. Cep3_C.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF16846. Cep3. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBF3B_YEAST
AccessioniPrimary (citable) accession number: P40969
Secondary accession number(s): D6VZZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.