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Protein

Pre-mRNA-processing factor 17

Gene

CDC40

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function in the second step of pre-mRNA splicing. Regulatory protein involved in replication and mitotic spindle formation and/or maintenance. Required for initiation and completion of S-phase and for initiation and completion of DNA replication. Might be required for the maintenance of microtubules. Essential only at elevated temperatures.

GO - Biological processi

  • generation of catalytic spliceosome for second transesterification step Source: SGD
  • mRNA 3'-splice site recognition Source: SGD
  • mRNA branch site recognition Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-29914-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 17
Alternative name(s):
Cell division control protein 40
Gene namesi
Name:CDC40
Synonyms:PRP17, SLU4, XRS2
Ordered Locus Names:YDR364C
ORF Names:D9481.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR364C.
SGDiS000002772. CDC40.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: GO_Central
  • nuclear periphery Source: SGD
  • spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511441 – 455Pre-mRNA-processing factor 17Add BLAST455

Proteomic databases

MaxQBiP40968.
PRIDEiP40968.

PTM databases

iPTMnetiP40968.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP19P325237EBI-13834,EBI-493

Protein-protein interaction databases

BioGridi32421. 69 interactors.
DIPiDIP-1111N.
IntActiP40968. 7 interactors.
MINTiMINT-617580.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50n1-455[»]
5GMKelectron microscopy3.40n1-455[»]
ProteinModelPortaliP40968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati160 – 200WD 1Add BLAST41
Repeati204 – 243WD 2Add BLAST40
Repeati291 – 330WD 3Add BLAST40
Repeati334 – 373WD 4Add BLAST40
Repeati379 – 422WD 5Add BLAST44
Repeati424 – 454WD 6Add BLAST31

Sequence similaritiesi

Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00530000063583.
HOGENOMiHOG000214672.
InParanoidiP40968.
KOiK12816.
OMAiDVAFNND.
OrthoDBiEOG092C2BKC.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR032847. PRPF17.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR22847:SF474. PTHR22847:SF474. 2 hits.
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH
60 70 80 90 100
FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKEDQ DIFVHALAED
110 120 130 140 150
NLDSEQIEVE EVSHFYGKSE KDYQGRGYLY PPNDVDVDLR EERISFRCYL
160 170 180 190 200
PKKVIRNYPG HPEGTTALKF LPKTGHLILS GGNDHTIKIW DFYHDYECLR
210 220 230 240 250
DFQGHNKPIK ALRFTEDCQS FLSSSFDRSV KIWDTETGKV KTRLHLNSTP
260 270 280 290 300
ADVESRPTNP HEFIVGLSNS KILHYDDRVS ENQGLVQTYD HHLSSILALK
310 320 330 340 350
YFPDGSKFIS SSEDKTVRIW ENQINVPIKQ ISDTAQHSMP FLNVHPSQNY
360 370 380 390 400
FCAQSMDNRI YSFSLKPKYK RHPKKIFKGH SSAGYGISLA FSGDGRYICS
410 420 430 440 450
GDSKSRLFTW DWNTSRLLNN IKIPGNKPIT QVDWHPQETS KVICSGAAGK

IYVCD
Length:455
Mass (Da):52,048
Last modified:July 15, 1998 - v2
Checksum:i40AED5DB35319147
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti390A → T in AAA86875 (PubMed:7753021).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17018 Genomic DNA. Translation: AAA86875.1.
U28373 Genomic DNA. Translation: AAB64800.1.
BK006938 Genomic DNA. Translation: DAA12203.1.
PIRiS61159.
RefSeqiNP_010652.3. NM_001180672.3.

Genome annotation databases

EnsemblFungiiYDR364C; YDR364C; YDR364C.
GeneIDi851968.
KEGGisce:YDR364C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17018 Genomic DNA. Translation: AAA86875.1.
U28373 Genomic DNA. Translation: AAB64800.1.
BK006938 Genomic DNA. Translation: DAA12203.1.
PIRiS61159.
RefSeqiNP_010652.3. NM_001180672.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50n1-455[»]
5GMKelectron microscopy3.40n1-455[»]
ProteinModelPortaliP40968.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32421. 69 interactors.
DIPiDIP-1111N.
IntActiP40968. 7 interactors.
MINTiMINT-617580.

PTM databases

iPTMnetiP40968.

Proteomic databases

MaxQBiP40968.
PRIDEiP40968.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR364C; YDR364C; YDR364C.
GeneIDi851968.
KEGGisce:YDR364C.

Organism-specific databases

EuPathDBiFungiDB:YDR364C.
SGDiS000002772. CDC40.

Phylogenomic databases

GeneTreeiENSGT00530000063583.
HOGENOMiHOG000214672.
InParanoidiP40968.
KOiK12816.
OMAiDVAFNND.
OrthoDBiEOG092C2BKC.

Enzyme and pathway databases

BioCyciYEAST:G3O-29914-MONOMER.

Miscellaneous databases

PROiP40968.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR032847. PRPF17.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR22847:SF474. PTHR22847:SF474. 2 hits.
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP17_YEAST
AccessioniPrimary (citable) accession number: P40968
Secondary accession number(s): D6VSZ3, Q06341
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.