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Protein

MutS protein homolog 4

Gene

MSH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in meiotic recombination. Facilitate crossovers between homologs during meiosis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi634 – 641ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: SGD
  • mismatched DNA binding Source: GO_Central

GO - Biological processi

  • mismatch repair Source: GO_Central
  • reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30451-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
MutS protein homolog 4
Gene namesi
Name:MSH4
Ordered Locus Names:YFL003C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL003C.
SGDiS000001891. MSH4.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001152011 – 878MutS protein homolog 4Add BLAST878

Proteomic databases

PRIDEiP40965.

Interactioni

Subunit structurei

Heterooligomer of MSH4 and MSH5.

Binary interactionsi

WithEntry#Exp.IntActNotes
MSH5Q121753EBI-11371,EBI-11377

Protein-protein interaction databases

BioGridi31143. 88 interactors.
DIPiDIP-2426N.
IntActiP40965. 5 interactors.
MINTiMINT-565232.

Structurei

3D structure databases

ProteinModelPortaliP40965.
SMRiP40965.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074897.
HOGENOMiHOG000094365.
InParanoidiP40965.
KOiK08740.
OMAiWTPQMGY.
OrthoDBiEOG092C1JVU.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 2 hits.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL
60 70 80 90 100
KKKTLQIAET TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP
110 120 130 140 150
SIDCVLCCIY EVPRDISTRI GLCIINCNTG QMYLSDFMDS QIYIRVVHKL
160 170 180 190 200
QIYQPTEILI PSSSLAPTVS KLATMIKFNV AETVKIEEGS RKCFNSQDGL
210 220 230 240 250
AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI ISKSSRNLNA
260 270 280 290 300
FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM
310 320 330 340 350
GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD
360 370 380 390 400
KLFSRLLCIN HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI
410 420 430 440 450
SETKKIFNND AIMEIEKLIN SCINEDCVWA SSAIQLLNQR SYAVKSDSNG
460 470 480 490 500
LLDVSRQIYK EVKEEFFREV EDLTAKNKIN LDHNYDSARG FYLRIKRQEF
510 520 530 540 550
TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME EILLLSEETV
560 570 580 590 600
DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI
610 620 630 640 650
RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI
660 670 680 690 700
CIMAQMGSGI PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL
710 720 730 740 750
DDINTETLLI LDELGRGSSI ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI
760 770 780 790 800
PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ LTQKSVAIEN SGIRVVKKIF
810 820 830 840 850
NPDIIAEAYN IHSLLKIAKA RTENEDSNGV VDQKTINQMK RIHNLVAILK
860 870
ECAGNEKEPL TLGKLKEINS DFIENFEE
Length:878
Mass (Da):99,225
Last modified:October 5, 2010 - v2
Checksum:iBBA1CABF1A08954C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti811 – 812IH → MD in AAA65234 (PubMed:8001134).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13999 Genomic DNA. Translation: AAA65234.1.
D50617 Genomic DNA. Translation: BAA09235.1.
BK006940 Genomic DNA. Translation: DAA12436.1.
PIRiA55201.
RefSeqiNP_116652.1. NM_001179963.1.

Genome annotation databases

EnsemblFungiiBAA09235; BAA09235; BAA09235.
YFL003C; YFL003C; YFL003C.
GeneIDi850545.
KEGGisce:YFL003C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13999 Genomic DNA. Translation: AAA65234.1.
D50617 Genomic DNA. Translation: BAA09235.1.
BK006940 Genomic DNA. Translation: DAA12436.1.
PIRiA55201.
RefSeqiNP_116652.1. NM_001179963.1.

3D structure databases

ProteinModelPortaliP40965.
SMRiP40965.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31143. 88 interactors.
DIPiDIP-2426N.
IntActiP40965. 5 interactors.
MINTiMINT-565232.

Proteomic databases

PRIDEiP40965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09235; BAA09235; BAA09235.
YFL003C; YFL003C; YFL003C.
GeneIDi850545.
KEGGisce:YFL003C.

Organism-specific databases

EuPathDBiFungiDB:YFL003C.
SGDiS000001891. MSH4.

Phylogenomic databases

GeneTreeiENSGT00550000074897.
HOGENOMiHOG000094365.
InParanoidiP40965.
KOiK08740.
OMAiWTPQMGY.
OrthoDBiEOG092C1JVU.

Enzyme and pathway databases

BioCyciYEAST:G3O-30451-MONOMER.

Miscellaneous databases

PROiP40965.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 2 hits.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSH4_YEAST
AccessioniPrimary (citable) accession number: P40965
Secondary accession number(s): D6VTM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Two distinct classes of crossovers have been demonstrated in budding yeast. Class I is MSH4/MSH5 dependent and exhibits crossover interference. Class II is MUS81/MMS4 dependent and exhibits no interference.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.