P40965 (MSH4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MutS protein homolog 4

Gene names

Name:	MSH4
Ordered Locus Names:	YFL003C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in meiotic recombination. Facilitate crossovers between homologs during meiosis.
Subunit structure	Heterooligomer of MSH4 and MSH5.
Miscellaneous	Two distinct classes of crossovers have been demonstrated in budding yeast. Class I is MSH4/MSH5 dependent and exhibits crossover interference. Class II is MUS81/MMS4 dependent and exhibits no interference.
Sequence similarities	Belongs to the DNA mismatch repair mutS family.

Ontologies

Keywords
Biological process	Meiosis
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chiasma assembly Inferred from Biological aspect of Ancestor. Source: RefGenome homologous chromosome segregation Inferred from Biological aspect of Ancestor. Source: RefGenome mismatch repair Inferred from electronic annotation. Source: InterPro reciprocal meiotic recombination Inferred from mutant phenotype. Source: SGD
Cellular component	synaptonemal complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from Biological aspect of Ancestor. Source: RefGenome mismatched DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MSH5	Q12175	3	EBI-11371,EBI-11377

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	MutS protein homolog 4		PRO_0000115201
Regions
	Nucleotide binding	634 – 641	8	ATP Potential
Experimental info
	Sequence conflict	811 – 812	2	IH → MD in AAA65234. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40965 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: BBA1CABF1A08954C	FASTA	878	99,225
	10 20 30 40 50 60 MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL KKKTLQIAET 70 80 90 100 110 120 TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP SIDCVLCCIY EVPRDISTRI 130 140 150 160 170 180 GLCIINCNTG QMYLSDFMDS QIYIRVVHKL QIYQPTEILI PSSSLAPTVS KLATMIKFNV 190 200 210 220 230 240 AETVKIEEGS RKCFNSQDGL AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI 250 260 270 280 290 300 ISKSSRNLNA FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM 310 320 330 340 350 360 GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD KLFSRLLCIN 370 380 390 400 410 420 HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI SETKKIFNND AIMEIEKLIN 430 440 450 460 470 480 SCINEDCVWA SSAIQLLNQR SYAVKSDSNG LLDVSRQIYK EVKEEFFREV EDLTAKNKIN 490 500 510 520 530 540 LDHNYDSARG FYLRIKRQEF TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME 550 560 570 580 590 600 EILLLSEETV DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI 610 620 630 640 650 660 RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI CIMAQMGSGI 670 680 690 700 710 720 PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL DDINTETLLI LDELGRGSSI 730 740 750 760 770 780 ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ 790 800 810 820 830 840 LTQKSVAIEN SGIRVVKKIF NPDIIAEAYN IHSLLKIAKA RTENEDSNGV VDQKTINQMK 850 860 870 RIHNLVAILK ECAGNEKEPL TLGKLKEINS DFIENFEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction." Ross-Macdonald P., Roeder G.S. Cell 79:1069-1080(1994) [PubMed: 8001134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI." Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T., Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T., Murakami Y. Yeast 12:77-84(1996) [PubMed: 8789262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Conserved properties between functionally distinct MutS homologs in yeast." Pochart P., Woltering D., Hollingsworth N.M. J. Biol. Chem. 272:30345-30349(1997) [PubMed: 9374523] [Abstract] Cited for: INTERACTION WITH MSH5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13999 Genomic DNA. Translation: AAA65234.1. D50617 Genomic DNA. Translation: BAA09235.1. BK006940 Genomic DNA. Translation: DAA12436.1.
PIR	A55201.
RefSeq	NP_116652.1. NM_001179963.1.
3D structure databases
ProteinModelPortal	P40965.
SMR	P40965. Positions 704-755.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2426N.
IntAct	P40965. 5 interactions.
MINT	MINT-565232.
STRING	P40965.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL003C; YFL003C; YFL003C.
GeneID	850545.
KEGG	sce:YFL003C.
Organism-specific databases
SGD	S000001891. MSH4.
Phylogenomic databases
eggNOG	fuNOG06823.
GeneTree	EFGT00050000003033.
HOGENOM	HBG203600.
OMA	CGLATYS.
OrthoDB	EOG4VHPFT.
Gene expression databases
ArrayExpress	P40965.
Genevestigator	P40965.
GermOnline	YFL003C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000432. DNA_mismatch_repair_MutS_C. IPR007861. DNA_mismatch_repair_MutS_clamp. IPR007860. DNA_mismatch_repair_MutS_connt. IPR007696. DNA_mismatch_repair_MutS_core. [Graphical view]
KO	K08740.
Pfam	PF05188. MutS_II. 1 hit. PF05192. MutS_III. 1 hit. PF05190. MutS_IV. 1 hit. PF00488. MutS_V. 1 hit. [Graphical view]
SMART	SM00534. MUTSac. 1 hit. SM00533. MUTSd. 1 hit. [Graphical view]
SUPFAM	SSF53150. DNA_mismatch_repair_MutS_connt. 1 hit. SSF48334. DNA_repair_MutS_domIII. 1 hit.
PROSITE	PS00486. DNA_MISMATCH_REPAIR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSH4_YEAST

Accession

Primary (citable) accession number: P40965
Secondary accession number(s): D6VTM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 5, 2010
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program