Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histone acetyltransferase SAS2

Gene

SAS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone acetyltransferase (HAT) subunit of the SAS complex, a multiprotein complex that acetylates 'Lys-16' of histone H4 and 'Lys-14' of histone H3. The SAS complex is however unable to acetylate nucleosomal histones. The complex is involved in transcriptional silencing at telomeres and at HML locus. Also involved in rDNA silencing and G0 control.2 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei242Proton donor/acceptorBy similarity1
Binding sitei246Acetyl-CoABy similarity1
Binding sitei323Acetyl-CoABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri100 – 126C2HC MYST-typePROSITE-ProRule annotationAdd BLAST27

GO - Molecular functioni

  • acetyltransferase activity Source: SGD
  • histone acetyltransferase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chromatin silencing at telomere Source: SGD
  • histone acetylation Source: SGD
  • histone exchange Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Chromatin regulator, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32820-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase SAS2 (EC:2.3.1.481 Publication)
Alternative name(s):
Something about silencing protein 2
Gene namesi
Name:SAS2
Synonyms:ESO1
Ordered Locus Names:YMR127C
ORF Names:YM9553.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR127C.
SGDiS000004734. SAS2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear chromatin Source: SGD
  • nuclear chromosome, telomeric region Source: SGD
  • SAS acetyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106C → L: Loss of function. 2 Publications1
Mutagenesisi168K → A, Q or R: Abolishes catalytic activity. 1
Mutagenesisi213 – 214PP → AV: Loss of function. 1 Publication2
Mutagenesisi216 – 217QR → AA: Abolishes silencing activity. 1 Publication2
Mutagenesisi219 – 221GLG → AAA: Does not affect silencing activity. 1 Publication3
Mutagenesisi224 – 225LI → AA: Does not affect silencing activity. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000515781 – 338Histone acetyltransferase SAS2Add BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei168N6-acetyllysine; by autocatalysis1 Publication1

Post-translational modificationi

Autoacetylation at Lys-168 is required for proper function.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP40963.

PTM databases

iPTMnetiP40963.

Interactioni

Subunit structurei

Interacts with CAC1. Component of the SAS complex, at least composed of SAS2, SAS4 and SAS5. These three proteins constitute the core of the complex and are sufficient to acetylate histones. SAS4 is essential for HAT activity of the complex, while SAS5 is required for maxiaml HAT activity.1 Publication1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASF1P324474EBI-16476,EBI-3003
RLF2Q124954EBI-16476,EBI-3913
SAS4Q040037EBI-16476,EBI-38500

Protein-protein interaction databases

BioGridi35304. 87 interactors.
DIPiDIP-4601N.
IntActiP40963. 7 interactors.
MINTiMINT-497812.

Structurei

3D structure databases

ProteinModelPortaliP40963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 338MYST-type HATPROSITE-ProRule annotationAdd BLAST294

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 211Acetyl-CoA bindingBy similarity3
Regioni216 – 222Acetyl-CoA bindingBy similarity7

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated
Contains 1 C2HC MYST-type zinc finger.PROSITE-ProRule annotation
Contains 1 MYST-type HAT (histone acetyltransferase) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri100 – 126C2HC MYST-typePROSITE-ProRule annotationAdd BLAST27

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00730000114398.
HOGENOMiHOG000066050.
InParanoidiP40963.
KOiK11401.
OMAiPLSPYGK.
OrthoDBiEOG092C3GJL.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR002717. HAT_MYST-type.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSLSQSLT ATTQKLKGKK NGGKGKNKPS AKIKKTQKEM LYGILNERNI
60 70 80 90 100
RQIQFGLNKK FSTWYGSAVY FDPETKRLGC SETKGQLSSV SNSQYWLDTL
110 120 130 140 150
FVCEYCFKYT DDQTRFVGHV ASCPFQYRVP GKIKYKSPEY TIRRVKGSKY
160 170 180 190 200
QLFCQCLCLF TKLYLDNKSM YFKVDHYEFY IVYETGSTKP MGFFSKDLVS
210 220 230 240 250
YQQNNLACIL IFPPYQRRGL GLLLIEFSYK LSQLEGVISG PEVPLSPFGL
260 270 280 290 300
IGYLKYWSQI LCWHLIEGDL AHYDKVTLED LSIVTGMRVN DVILTLKHLN
310 320 330
CIGENNQIYL QSLNSWLKLH GTKRNWFKLK DEYLLIDD
Length:338
Mass (Da):39,206
Last modified:February 1, 1995 - v1
Checksum:i22CBDC0D1B62A947
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14548 Genomic DNA. Translation: AAA21555.1.
Z48622 Genomic DNA. Translation: CAA88552.1.
BK006946 Genomic DNA. Translation: DAA10024.1.
PIRiS48299.
RefSeqiNP_013846.1. NM_001182628.1.

Genome annotation databases

EnsemblFungiiYMR127C; YMR127C; YMR127C.
GeneIDi855157.
KEGGisce:YMR127C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14548 Genomic DNA. Translation: AAA21555.1.
Z48622 Genomic DNA. Translation: CAA88552.1.
BK006946 Genomic DNA. Translation: DAA10024.1.
PIRiS48299.
RefSeqiNP_013846.1. NM_001182628.1.

3D structure databases

ProteinModelPortaliP40963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35304. 87 interactors.
DIPiDIP-4601N.
IntActiP40963. 7 interactors.
MINTiMINT-497812.

PTM databases

iPTMnetiP40963.

Proteomic databases

PRIDEiP40963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR127C; YMR127C; YMR127C.
GeneIDi855157.
KEGGisce:YMR127C.

Organism-specific databases

EuPathDBiFungiDB:YMR127C.
SGDiS000004734. SAS2.

Phylogenomic databases

GeneTreeiENSGT00730000114398.
HOGENOMiHOG000066050.
InParanoidiP40963.
KOiK11401.
OMAiPLSPYGK.
OrthoDBiEOG092C3GJL.

Enzyme and pathway databases

BioCyciYEAST:G3O-32820-MONOMER.

Miscellaneous databases

PROiP40963.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR002717. HAT_MYST-type.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAS2_YEAST
AccessioniPrimary (citable) accession number: P40963
Secondary accession number(s): D6VZV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.