Mitotic spindle checkpoint component MAD2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P40958 (MAD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitotic spindle checkpoint component MAD2
Short name=Mitotic MAD2 protein

Gene names

Name:	MAD2
Ordered Locus Names:	YJL030W
ORF Names:	J1256

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. Ref.1 Ref.10
Subunit structure	Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. Interacts with CDC20 and BUB3. Ref.6 Ref.7 Ref.8 Ref.10
Subcellular location	Nucleus.
Miscellaneous	Present with 1110 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the MAD2 family. Contains 1 HORMA domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome decondensation Inferred from mutant phenotype PubMed 17151360. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic DNA integrity checkpoint Inferred from genetic interaction PubMed 16651657. Source: SGD mitotic spindle assembly checkpoint Inferred from mutant phenotype PubMed 10704439. Source: SGD
Cellular_component	mitotic checkpoint complex Inferred from direct assay Ref.8. Source: SGD nuclear pore Inferred from direct assay PubMed 12473689. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
BUB3	P26449	4	EBI-10362,EBI-3830
CDC20	P26309	6	EBI-10362,EBI-4212
MAD1	P40957	6	EBI-10362,EBI-10354
MAD3	P47074	3	EBI-10362,EBI-10369

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 196

196

Mitotic spindle checkpoint component MAD2

PRO_0000126115

Regions

Domain

8 – 192

185

HORMA

Sequences

Sequence

Length

Mass (Da)

Tools

P40958 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: EFE59916C5720644
Show »

FASTA

196

22,284

        10         20         30         40         50         60 
MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT HDDELKDYIR 

        70         80         90        100        110        120 
KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH ISGNSNGQDD VVDLNTTQSQ 

       130        140        150        160        170        180 
IRALIRQITS SVTFLPELTK EGGYTFTVLA YTDADAKVPL EWADSNSKEI PDGEVVQFKT 

       190 
FSTNDHKVGA QVSYKY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase." Li R., Havel C., Watson J.A., Murray A.W. Nature 366:82-84(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Feedback control of mitosis in budding yeast." Li R., Murray A.W. Cell 66:519-531(1991) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Budding yeast Cdc20: a target of the spindle checkpoint." Hwang L.H., Lau L.F., Smith D.L., Mistrot C.A., Hardwick K.G., Hwang E.S., Amon A., Murray A.W. Science 279:1041-1044(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDC20.
[7]	"The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins." Chen R.H., Brady D.M., Smith D., Murray A.W., Hardwick K.G. Mol. Biol. Cell 10:2607-2618(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAD1.
[8]	"Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores." Fraschini R., Beretta A., Sironi L., Musacchio A., Lucchini G., Piatti S. EMBO J. 20:6648-6659(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BUB3, IDENTIFICATION IN THE MCC COMPLEX.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae." Poddar A., Stukenberg P.T., Burke D.J. Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14132 Genomic DNA. Translation: AAA21385.1. Z49305 Genomic DNA. Translation: CAA89321.1. AY558243 Genomic DNA. Translation: AAS56569.1. BK006943 Genomic DNA. Translation: DAA08768.1.
PIR	S48302.
RefSeq	NP_012504.3. NM_001181464.3.
3D structure databases
ProteinModelPortal	P40958.
SMR	P40958. Positions 8-195.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-815N.
IntAct	P40958. 30 interactions.
MINT	MINT-401796.
STRING	4932.YJL030W.
Proteomic databases
PaxDb	P40958.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL030W; YJL030W; YJL030W.
GeneID	853422.
KEGG	sce:YJL027C. sce:YJL030W.
Organism-specific databases
CYGD	YJL030w.
SGD	S000003567. MAD2.
Phylogenomic databases
eggNOG	NOG263853.
GeneTree	ENSGT00390000007908.
HOGENOM	HOG000199586.
KO	K02537.
OMA	EIRDVMR.
OrthoDB	EOG4BK8D8.
Gene expression databases
Genevestigator	P40958.
GermOnline	YJL030W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.900.10. 1 hit.
InterPro	IPR003511. HORMA_DNA-bd. IPR027097. Mad2. [Graphical view]
PANTHER	PTHR11842:SF11. PTHR11842:SF11. 1 hit.
Pfam	PF02301. HORMA. 1 hit. [Graphical view]
SUPFAM	SSF56019. HORMA_DNA_bd. 1 hit.
PROSITE	PS50815. HORMA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973948.

Entry information

Entry name

MAD2_YEAST

Accession

Primary (citable) accession number: P40958
Secondary accession number(s): D6VWF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents