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Protein

Mitotic spindle checkpoint component MAD2

Gene

MAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.2 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome decondensation Source: SGD
  • mitotic DNA integrity checkpoint Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31499-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint component MAD2
Short name:
Mitotic MAD2 protein
Gene namesi
Name:MAD2
Ordered Locus Names:YJL030W
ORF Names:J1256
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL030w.
EuPathDBiFungiDB:YJL030W.
SGDiS000003567. MAD2.

Subcellular locationi

GO - Cellular componenti

  • mitotic checkpoint complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Mitotic spindle checkpoint component MAD2PRO_0000126115Add
BLAST

Proteomic databases

MaxQBiP40958.
PaxDbiP40958.

Expressioni

Gene expression databases

GenevestigatoriP40958.

Interactioni

Subunit structurei

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. Interacts with CDC20 and BUB3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB3P264494EBI-10362,EBI-3830
CDC20P263096EBI-10362,EBI-4212
MAD1P409576EBI-10362,EBI-10354
MAD3P470743EBI-10362,EBI-10369

Protein-protein interaction databases

BioGridi33729. 245 interactions.
DIPiDIP-815N.
IntActiP40958. 30 interactions.
MINTiMINT-401796.
STRINGi4932.YJL030W.

Structurei

3D structure databases

ProteinModelPortaliP40958.
SMRiP40958. Positions 8-195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 192185HORMAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MAD2 family.Curated
Contains 1 HORMA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG263853.
GeneTreeiENSGT00390000007908.
HOGENOMiHOG000199586.
InParanoidiP40958.
KOiK02537.
OMAiVHRIEAM.
OrthoDBiEOG7FZ09D.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_DNA-bd.
IPR027097. Mad2.
[Graphical view]
PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT
60 70 80 90 100
HDDELKDYIR KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH
110 120 130 140 150
ISGNSNGQDD VVDLNTTQSQ IRALIRQITS SVTFLPELTK EGGYTFTVLA
160 170 180 190
YTDADAKVPL EWADSNSKEI PDGEVVQFKT FSTNDHKVGA QVSYKY
Length:196
Mass (Da):22,284
Last modified:February 1, 1995 - v1
Checksum:iEFE59916C5720644
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14132 Genomic DNA. Translation: AAA21385.1.
Z49305 Genomic DNA. Translation: CAA89321.1.
AY558243 Genomic DNA. Translation: AAS56569.1.
BK006943 Genomic DNA. Translation: DAA08768.1.
PIRiS48302.
RefSeqiNP_012504.3. NM_001181464.3.

Genome annotation databases

EnsemblFungiiYJL030W; YJL030W; YJL030W.
GeneIDi853422.
KEGGisce:YJL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14132 Genomic DNA. Translation: AAA21385.1.
Z49305 Genomic DNA. Translation: CAA89321.1.
AY558243 Genomic DNA. Translation: AAS56569.1.
BK006943 Genomic DNA. Translation: DAA08768.1.
PIRiS48302.
RefSeqiNP_012504.3. NM_001181464.3.

3D structure databases

ProteinModelPortaliP40958.
SMRiP40958. Positions 8-195.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33729. 245 interactions.
DIPiDIP-815N.
IntActiP40958. 30 interactions.
MINTiMINT-401796.
STRINGi4932.YJL030W.

Proteomic databases

MaxQBiP40958.
PaxDbiP40958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL030W; YJL030W; YJL030W.
GeneIDi853422.
KEGGisce:YJL030W.

Organism-specific databases

CYGDiYJL030w.
EuPathDBiFungiDB:YJL030W.
SGDiS000003567. MAD2.

Phylogenomic databases

eggNOGiNOG263853.
GeneTreeiENSGT00390000007908.
HOGENOMiHOG000199586.
InParanoidiP40958.
KOiK02537.
OMAiVHRIEAM.
OrthoDBiEOG7FZ09D.

Enzyme and pathway databases

BioCyciYEAST:G3O-31499-MONOMER.

Miscellaneous databases

NextBioi973948.
PROiP40958.

Gene expression databases

GenevestigatoriP40958.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_DNA-bd.
IPR027097. Mad2.
[Graphical view]
PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase."
    Li R., Havel C., Watson J.A., Murray A.W.
    Nature 366:82-84(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Feedback control of mitosis in budding yeast."
    Li R., Murray A.W.
    Cell 66:519-531(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: INTERACTION WITH CDC20.
  7. "The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins."
    Chen R.H., Brady D.M., Smith D., Murray A.W., Hardwick K.G.
    Mol. Biol. Cell 10:2607-2618(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAD1.
  8. "Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores."
    Fraschini R., Beretta A., Sironi L., Musacchio A., Lucchini G., Piatti S.
    EMBO J. 20:6648-6659(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BUB3, IDENTIFICATION IN THE MCC COMPLEX.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae."
    Poddar A., Stukenberg P.T., Burke D.J.
    Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.

Entry informationi

Entry nameiMAD2_YEAST
AccessioniPrimary (citable) accession number: P40958
Secondary accession number(s): D6VWF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 29, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1110 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.