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Protein

Mitotic spindle checkpoint component MAD2

Gene

MAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.2 Publications

Miscellaneous

Present with 1110 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome decondensation Source: SGD
  • mitotic DNA integrity checkpoint Source: SGD
  • mitotic spindle assembly checkpoint Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31499-MONOMER
ReactomeiR-SCE-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
R-SCE-141430 Inactivation of APC/C via direct inhibition of the APC/C complex

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint component MAD2
Short name:
Mitotic MAD2 protein
Gene namesi
Name:MAD2
Ordered Locus Names:YJL030W
ORF Names:J1256
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL030W
SGDiS000003567 MAD2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001261151 – 196Mitotic spindle checkpoint component MAD2Add BLAST196

Proteomic databases

MaxQBiP40958
PaxDbiP40958
PRIDEiP40958

Interactioni

Subunit structurei

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. Interacts with CDC20 and BUB3.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33729, 300 interactors
DIPiDIP-815N
IntActiP40958, 33 interactors
MINTiP40958
STRINGi4932.YJL030W

Structurei

3D structure databases

ProteinModelPortaliP40958
SMRiP40958
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 192HORMAPROSITE-ProRule annotationAdd BLAST185

Sequence similaritiesi

Belongs to the MAD2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007908
HOGENOMiHOG000199586
InParanoidiP40958
KOiK02537
OMAiSEVPVEW
OrthoDBiEOG092C4F48

Family and domain databases

Gene3Di3.30.900.10, 1 hit
InterProiView protein in InterPro
IPR003511 HORMA_dom
IPR036570 HORMA_dom_sf
IPR027097 Mad2
PANTHERiPTHR11842:SF11 PTHR11842:SF11, 1 hit
PfamiView protein in Pfam
PF02301 HORMA, 1 hit
SUPFAMiSSF56019 SSF56019, 1 hit
PROSITEiView protein in PROSITE
PS50815 HORMA, 1 hit

Sequencei

Sequence statusi: Complete.

P40958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT
60 70 80 90 100
HDDELKDYIR KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH
110 120 130 140 150
ISGNSNGQDD VVDLNTTQSQ IRALIRQITS SVTFLPELTK EGGYTFTVLA
160 170 180 190
YTDADAKVPL EWADSNSKEI PDGEVVQFKT FSTNDHKVGA QVSYKY
Length:196
Mass (Da):22,284
Last modified:February 1, 1995 - v1
Checksum:iEFE59916C5720644
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14132 Genomic DNA Translation: AAA21385.1
Z49305 Genomic DNA Translation: CAA89321.1
AY558243 Genomic DNA Translation: AAS56569.1
BK006943 Genomic DNA Translation: DAA08768.1
PIRiS48302
RefSeqiNP_012504.3, NM_001181464.3

Genome annotation databases

EnsemblFungiiYJL030W; YJL030W; YJL030W
GeneIDi853422
KEGGisce:YJL030W

Similar proteinsi

Entry informationi

Entry nameiMAD2_YEAST
AccessioniPrimary (citable) accession number: P40958
Secondary accession number(s): D6VWF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 25, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health