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P40958 (MAD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitotic spindle checkpoint component MAD2

Short name=Mitotic MAD2 protein
Gene names
Name:MAD2
Ordered Locus Names:YJL030W
ORF Names:J1256
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. Ref.1 Ref.10

Subunit structure

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. Interacts with CDC20 and BUB3. Ref.6 Ref.7 Ref.8 Ref.10

Subcellular location

Nucleus.

Miscellaneous

Present with 1110 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the MAD2 family.

Contains 1 HORMA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 196196Mitotic spindle checkpoint component MAD2
PRO_0000126115

Regions

Domain8 – 192185HORMA

Sequences

Sequence LengthMass (Da)Tools
P40958 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: EFE59916C5720644

FASTA19622,284
        10         20         30         40         50         60 
MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT HDDELKDYIR 

        70         80         90        100        110        120 
KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH ISGNSNGQDD VVDLNTTQSQ 

       130        140        150        160        170        180 
IRALIRQITS SVTFLPELTK EGGYTFTVLA YTDADAKVPL EWADSNSKEI PDGEVVQFKT 

       190 
FSTNDHKVGA QVSYKY 

« Hide

References

« Hide 'large scale' references
[1]"The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase."
Li R., Havel C., Watson J.A., Murray A.W.
Nature 366:82-84(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Feedback control of mitosis in budding yeast."
Li R., Murray A.W.
Cell 66:519-531(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Budding yeast Cdc20: a target of the spindle checkpoint."
Hwang L.H., Lau L.F., Smith D.L., Mistrot C.A., Hardwick K.G., Hwang E.S., Amon A., Murray A.W.
Science 279:1041-1044(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDC20.
[7]"The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins."
Chen R.H., Brady D.M., Smith D., Murray A.W., Hardwick K.G.
Mol. Biol. Cell 10:2607-2618(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAD1.
[8]"Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores."
Fraschini R., Beretta A., Sironi L., Musacchio A., Lucchini G., Piatti S.
EMBO J. 20:6648-6659(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BUB3, IDENTIFICATION IN THE MCC COMPLEX.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae."
Poddar A., Stukenberg P.T., Burke D.J.
Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14132 Genomic DNA. Translation: AAA21385.1.
Z49305 Genomic DNA. Translation: CAA89321.1.
AY558243 Genomic DNA. Translation: AAS56569.1.
BK006943 Genomic DNA. Translation: DAA08768.1.
PIRS48302.
RefSeqNP_012504.3. NM_001181464.3.

3D structure databases

ProteinModelPortalP40958.
SMRP40958. Positions 8-195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33729. 244 interactions.
DIPDIP-815N.
IntActP40958. 30 interactions.
MINTMINT-401796.
STRING4932.YJL030W.

Proteomic databases

PaxDbP40958.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL030W; YJL030W; YJL030W.
GeneID853422.
KEGGsce:YJL030W.

Organism-specific databases

CYGDYJL030w.
SGDS000003567. MAD2.

Phylogenomic databases

eggNOGNOG263853.
GeneTreeENSGT00390000007908.
HOGENOMHOG000199586.
KOK02537.
OMAFSTDVHR.
OrthoDBEOG7FZ09D.

Enzyme and pathway databases

BioCycYEAST:G3O-31499-MONOMER.

Gene expression databases

GenevestigatorP40958.

Family and domain databases

Gene3D3.30.900.10. 1 hit.
InterProIPR003511. HORMA_DNA-bd.
IPR027097. Mad2.
[Graphical view]
PANTHERPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMSSF56019. SSF56019. 1 hit.
PROSITEPS50815. HORMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973948.
PROP40958.

Entry information

Entry nameMAD2_YEAST
AccessionPrimary (citable) accession number: P40958
Secondary accession number(s): D6VWF2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 19, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families