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P40958

- MAD2_YEAST

UniProt

P40958 - MAD2_YEAST

Protein

Mitotic spindle checkpoint component MAD2

Gene

MAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. chromosome decondensation Source: SGD
    2. mitotic DNA integrity checkpoint Source: SGD
    3. mitotic nuclear division Source: UniProtKB-KW
    4. mitotic spindle assembly checkpoint Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31499-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitotic spindle checkpoint component MAD2
    Short name:
    Mitotic MAD2 protein
    Gene namesi
    Name:MAD2
    Ordered Locus Names:YJL030W
    ORF Names:J1256
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL030w.
    SGDiS000003567. MAD2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitotic checkpoint complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196Mitotic spindle checkpoint component MAD2PRO_0000126115Add
    BLAST

    Proteomic databases

    MaxQBiP40958.
    PaxDbiP40958.

    Expressioni

    Gene expression databases

    GenevestigatoriP40958.

    Interactioni

    Subunit structurei

    Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. Interacts with CDC20 and BUB3.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUB3P264494EBI-10362,EBI-3830
    CDC20P263096EBI-10362,EBI-4212
    MAD1P409576EBI-10362,EBI-10354
    MAD3P470743EBI-10362,EBI-10369

    Protein-protein interaction databases

    BioGridi33729. 245 interactions.
    DIPiDIP-815N.
    IntActiP40958. 30 interactions.
    MINTiMINT-401796.
    STRINGi4932.YJL030W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40958.
    SMRiP40958. Positions 8-195.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 192185HORMAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MAD2 family.Curated
    Contains 1 HORMA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG263853.
    GeneTreeiENSGT00390000007908.
    HOGENOMiHOG000199586.
    KOiK02537.
    OMAiFSTDVHR.
    OrthoDBiEOG7FZ09D.

    Family and domain databases

    Gene3Di3.30.900.10. 1 hit.
    InterProiIPR003511. HORMA_DNA-bd.
    IPR027097. Mad2.
    [Graphical view]
    PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
    PfamiPF02301. HORMA. 1 hit.
    [Graphical view]
    SUPFAMiSSF56019. SSF56019. 1 hit.
    PROSITEiPS50815. HORMA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40958-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT    50
    HDDELKDYIR KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH 100
    ISGNSNGQDD VVDLNTTQSQ IRALIRQITS SVTFLPELTK EGGYTFTVLA 150
    YTDADAKVPL EWADSNSKEI PDGEVVQFKT FSTNDHKVGA QVSYKY 196
    Length:196
    Mass (Da):22,284
    Last modified:February 1, 1995 - v1
    Checksum:iEFE59916C5720644
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14132 Genomic DNA. Translation: AAA21385.1.
    Z49305 Genomic DNA. Translation: CAA89321.1.
    AY558243 Genomic DNA. Translation: AAS56569.1.
    BK006943 Genomic DNA. Translation: DAA08768.1.
    PIRiS48302.
    RefSeqiNP_012504.3. NM_001181464.3.

    Genome annotation databases

    EnsemblFungiiYJL030W; YJL030W; YJL030W.
    GeneIDi853422.
    KEGGisce:YJL030W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14132 Genomic DNA. Translation: AAA21385.1 .
    Z49305 Genomic DNA. Translation: CAA89321.1 .
    AY558243 Genomic DNA. Translation: AAS56569.1 .
    BK006943 Genomic DNA. Translation: DAA08768.1 .
    PIRi S48302.
    RefSeqi NP_012504.3. NM_001181464.3.

    3D structure databases

    ProteinModelPortali P40958.
    SMRi P40958. Positions 8-195.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33729. 245 interactions.
    DIPi DIP-815N.
    IntActi P40958. 30 interactions.
    MINTi MINT-401796.
    STRINGi 4932.YJL030W.

    Proteomic databases

    MaxQBi P40958.
    PaxDbi P40958.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL030W ; YJL030W ; YJL030W .
    GeneIDi 853422.
    KEGGi sce:YJL030W.

    Organism-specific databases

    CYGDi YJL030w.
    SGDi S000003567. MAD2.

    Phylogenomic databases

    eggNOGi NOG263853.
    GeneTreei ENSGT00390000007908.
    HOGENOMi HOG000199586.
    KOi K02537.
    OMAi FSTDVHR.
    OrthoDBi EOG7FZ09D.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31499-MONOMER.

    Miscellaneous databases

    NextBioi 973948.
    PROi P40958.

    Gene expression databases

    Genevestigatori P40958.

    Family and domain databases

    Gene3Di 3.30.900.10. 1 hit.
    InterProi IPR003511. HORMA_DNA-bd.
    IPR027097. Mad2.
    [Graphical view ]
    PANTHERi PTHR11842:SF11. PTHR11842:SF11. 1 hit.
    Pfami PF02301. HORMA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56019. SSF56019. 1 hit.
    PROSITEi PS50815. HORMA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase."
      Li R., Havel C., Watson J.A., Murray A.W.
      Nature 366:82-84(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Feedback control of mitosis in budding yeast."
      Li R., Murray A.W.
      Cell 66:519-531(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: INTERACTION WITH CDC20.
    7. "The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins."
      Chen R.H., Brady D.M., Smith D., Murray A.W., Hardwick K.G.
      Mol. Biol. Cell 10:2607-2618(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAD1.
    8. "Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores."
      Fraschini R., Beretta A., Sironi L., Musacchio A., Lucchini G., Piatti S.
      EMBO J. 20:6648-6659(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BUB3, IDENTIFICATION IN THE MCC COMPLEX.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae."
      Poddar A., Stukenberg P.T., Burke D.J.
      Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.

    Entry informationi

    Entry nameiMAD2_YEAST
    AccessioniPrimary (citable) accession number: P40958
    Secondary accession number(s): D6VWF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1110 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3