ID   XYNA_CALSR              Reviewed;         684 AA.
AC   P40944;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 100.
DE   RecName: Full=Endo-1,4-beta-xylanase A;
DE            Short=Xylanase A;
DE            EC=3.2.1.8;
DE   AltName: Full=1,4-beta-D-xylan xylanohydrolase A;
DE   Flags: Precursor;
GN   Name=xynA;
OS   Caldicellulosiruptor sp. (strain Rt8B.4).
OC   Bacteria; Bacillota; Clostridia; Caldicellulosiruptorales;
OC   Caldicellulosiruptoraceae; Caldicellulosiruptor.
OX   NCBI_TaxID=28238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8920183; DOI=10.1007/s002530050653;
RA   Dwivedi P.P., Gibbs M.D., Saul D.J., Bergquist P.L.;
RT   "Cloning, sequencing and overexpression in Escherichia coli of a xylanase
RT   gene, xynA from the thermophilic bacterium Rt8B.4 genus
RT   Caldicellulosiruptor.";
RL   Appl. Microbiol. Biotechnol. 45:86-93(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.;
CC         EC=3.2.1.8;
CC   -!- PATHWAY: Glycan degradation; xylan degradation.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 10 (cellulase F) family.
CC       {ECO:0000305}.
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DR   EMBL; L18965; AAB42044.1; -; Genomic_DNA.
DR   PIR; S41788; S41788.
DR   AlphaFoldDB; P40944; -.
DR   SMR; P40944; -.
DR   CAZy; CBM22; Carbohydrate-Binding Module Family 22.
DR   CAZy; GH10; Glycoside Hydrolase Family 10.
DR   UniPathway; UPA00114; -.
DR   GO; GO:0031176; F:endo-1,4-beta-xylanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR003305; CenC_carb-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR044846; GH10.
DR   InterPro; IPR031158; GH10_AS.
DR   InterPro; IPR001000; GH10_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR31490:SF88; BETA-XYLANASE; 1.
DR   PANTHER; PTHR31490; GLYCOSYL HYDROLASE; 1.
DR   Pfam; PF02018; CBM_4_9; 2.
DR   Pfam; PF00331; Glyco_hydro_10; 1.
DR   PRINTS; PR00134; GLHYDRLASE10.
DR   SMART; SM00633; Glyco_10; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR   PROSITE; PS00591; GH10_1; 1.
DR   PROSITE; PS51760; GH10_2; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Repeat; Signal; Xylan degradation.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000255"
FT   CHAIN           35..684
FT                   /note="Endo-1,4-beta-xylanase A"
FT                   /id="PRO_0000007981"
FT   DOMAIN          40..190
FT                   /note="CBM-cenC 1"
FT   DOMAIN          193..342
FT                   /note="CBM-cenC 2"
FT   DOMAIN          350..678
FT                   /note="GH10"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01096"
FT   ACT_SITE        490
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        598
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10061"
SQ   SEQUENCE   684 AA;  78354 MW;  0AE575F4FB4BA5E6 CRC64;
     MMRSLKSRKL VFILAMLFLI NAIVSLKFIT YSSANQLASK SKYIEYNFEN KSVSPLAKYP
     SNVVLKVTNS TCAEGTFSVL VAGRKNANDG VIVDITKFLD FSREYEVSFY VLQTTKKLQR
     ISVTLEILDS NDKNQVIAAE KVLLPNIWTK VSAKVQASNY KKAKRINLIV NMPTSKSDSF
     YIDLFTIKDL ENAYVLKQEN FENKNTGGFL PEDKNCKITL AKDRAYSSAY SLKVQPSQKT
     KNGKILFPIK GLLQKGGTYD FSLLVYQDSS KPVNFSAGIK LNDGKSTKEI VLAKQNVAPK
     KWTQLFATLD LDTRFSAKDV SFFVKPAAAI SYYLDLYSIS DENWGQPVPD YNLPSLCEKY
     KNYFKIGVAV PYRALTNPVD VEVIKRHFNS ITPENEMKPE SLQPYEGGFS FSIADEYVDF
     CKKDNISLRG HTLVWHQQTP SWFFTNPETG EKLTNSEKDK EILLDRLKKH IQTVVGRYKG
     KVYAWDVVNE AIDENQPDGY RRSDWYNILG PEYIEKAFIW AHEADPKAKL FYNDYSTEDP
     YKREFIYKLI KNLKAKGVPV HGVGLQCHIS LDWPDVSEIE ETVKLFSRIP GLEIHFTEID
     ISIAKNMTDD DAYNRYLLVQ QAQKLKAIFD VLKKYRNVVT SVTFWGLKDD YSWLRGDMPL
     LSDKDYQPKF AFWSLIDPSV VPKE
//