Reviewed, UniProtKB/Swiss-Prot P40944 (XYNA_CALSR)

Names and origin

Protein names

Recommended name:
    Endo-1,4-beta-xylanase A
      Short name=Xylanase A
    EC=3.2.1.8
Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase A

Gene names

Name:

xynA

Organism

Caldicellulosiruptor sp. (strain Rt8B.4)

Taxonomic identifier

28238 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 10 (cellulase F) family. Contains 2 CBM-cenC (cenC-type cellulose-binding) domains.

Ontologies

Keywords
Biological process	Xylan degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 684	650	Endo-1,4-beta-xylanase A		PRO_0000007981
Regions
	Domain	40 – 190	151	CBM-cenC 1
	Domain	193 – 342	150	CBM-cenC 2
Sites
	Active site	490	1	Proton donor By similarity
	Active site	598	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40944-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 0AE575F4FB4BA5E6	FASTA	684	78,354
	10 20 30 40 50 60 MMRSLKSRKL VFILAMLFLI NAIVSLKFIT YSSANQLASK SKYIEYNFEN KSVSPLAKYP 70 80 90 100 110 120 SNVVLKVTNS TCAEGTFSVL VAGRKNANDG VIVDITKFLD FSREYEVSFY VLQTTKKLQR 130 140 150 160 170 180 ISVTLEILDS NDKNQVIAAE KVLLPNIWTK VSAKVQASNY KKAKRINLIV NMPTSKSDSF 190 200 210 220 230 240 YIDLFTIKDL ENAYVLKQEN FENKNTGGFL PEDKNCKITL AKDRAYSSAY SLKVQPSQKT 250 260 270 280 290 300 KNGKILFPIK GLLQKGGTYD FSLLVYQDSS KPVNFSAGIK LNDGKSTKEI VLAKQNVAPK 310 320 330 340 350 360 KWTQLFATLD LDTRFSAKDV SFFVKPAAAI SYYLDLYSIS DENWGQPVPD YNLPSLCEKY 370 380 390 400 410 420 KNYFKIGVAV PYRALTNPVD VEVIKRHFNS ITPENEMKPE SLQPYEGGFS FSIADEYVDF 430 440 450 460 470 480 CKKDNISLRG HTLVWHQQTP SWFFTNPETG EKLTNSEKDK EILLDRLKKH IQTVVGRYKG 490 500 510 520 530 540 KVYAWDVVNE AIDENQPDGY RRSDWYNILG PEYIEKAFIW AHEADPKAKL FYNDYSTEDP 550 560 570 580 590 600 YKREFIYKLI KNLKAKGVPV HGVGLQCHIS LDWPDVSEIE ETVKLFSRIP GLEIHFTEID 610 620 630 640 650 660 ISIAKNMTDD DAYNRYLLVQ QAQKLKAIFD VLKKYRNVVT SVTFWGLKDD YSWLRGDMPL 670 680 LSDKDYQPKF AFWSLIDPSV VPKE

References

[1]

"Cloning, sequencing and overexpression in Escherichia coli of a xylanase gene, xynA from the thermophilic bacterium Rt8B.4 genus Caldicellulosiruptor." Dwivedi P.P., Gibbs M.D., Saul D.J., Bergquist P.L. Appl. Microbiol. Biotechnol. 45:86-93(1996) [PubMed: 8920183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18965 Genomic DNA. Translation: AAB42044.1.
PIR	S41788.
3D structure databases
HSSP	HSSP built from PDB template 1HIZ based on UniProtKB P40943.
ModBase	Search...
Protein family/group databases
CAZy	CBM22. Carbohydrate-Binding Module Family 22. GH10. Glycoside Hydrolase Family 10.
Family and domain databases
InterPro	IPR003305. CenC_carb_bd. IPR001000. Glyco_hydro_10. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02018. CBM_4_9. 2 hits. PF00331. Glyco_hydro_10. 1 hit. [Graphical view]
PRINTS	PR00134. GLHYDRLASE10.
SMART	SM00633. Glyco_10. 1 hit. [Graphical view]
PROSITE	PS00591. GLYCOSYL_HYDROL_F10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNA_CALSR

Accession

Primary (citable) accession number: P40944

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)