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P40942 (CEXY_CLOSR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thermostable celloxylanase
EC=3.2.1.4
EC=3.2.1.8
Gene names
Name:xynB
OrganismClostridium stercorarium
Taxonomic identifier1510 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › unclassified Ruminococcaceae

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Active toward xylan, carboxymethylcellulose, P-nitrophenyl-beta-D-xylopyranoside and P-nitrophenyl-beta-D-cellobioside.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Pathway

Glycan degradation; xylan degradation.

Sequence similarities

Belongs to the glycosyl hydrolase 10 (cellulase F) family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0.

Temperature dependence:

Optimum temperature is 80 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Thermostable celloxylanase
PRO_0000184061

Sites

Active site1851Proton donor By similarity
Active site2931Nucleophile By similarity

Secondary structure

1 ........................................................... 387
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40942 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: C7221BD5E32C8E48

FASTA38744,378
        10         20         30         40         50         60 
MNKFLNKKWS LILTMGGIFL MATLSLIFAT GKKAFNDQTS AEDIPSLAEA FRDYFPIGAA 

        70         80         90        100        110        120 
IEPGYTTGQI AELYKKHVNM LVAENAMKPA SLQPTEGNFQ WADADRIVQF AKENGMELRF 

       130        140        150        160        170        180 
HTLVWHNQTP TGFSLDKEGK PMVEETDPQK REENRKLLLQ RLENYIRAVV LRYKDDIKSW 

       190        200        210        220        230        240 
DVVNEVIEPN DPGGMRNSPW YQITGTEYIE VAFRATREAG GSDIKLYIND YNTDDPVKRD 

       250        260        270        280        290        300 
ILYELVKNLL EKGVPIDGVG HQTHIDIYNP PVERIIESIK KFAGLGLDNI ITELDMSIYS 

       310        320        330        340        350        360 
WNDRSDYGDS IPDYILTLQA KRYQELFDAL KENKDIVSAV VFWGISDKYS WLNGFPVKRT 

       370        380 
NAPLLFDRNF MPKPAFWAIV DPSRLRE

« Hide

References

[1]"Nucleotide sequence of the Clostridium stercorarium xynB gene encoding an extremely thermostable xylanase, and characterization of the translated product."
Fukumura M., Sakka K., Shimada K., Ohmiya K.
Biosci. Biotechnol. Biochem. 59:40-46(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: F-9.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D12504 Genomic DNA. Translation: BAA02069.1.
PIRJC2484.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DEPX-ray1.80A/B41-387[»]
ProteinModelPortalP40942.
SMRP40942. Positions 43-382.
ModBaseSearch...

Protein family/group databases

CAZyGH10. Glycoside Hydrolase Family 10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00114.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSPR00134. GLHYDRLASE10.
SMARTSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00591. GLYCOSYL_HYDROL_F10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP40942.

Entry information

Entry nameCEXY_CLOSR
AccessionPrimary (citable) accession number: P40942
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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