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Protein

Thermostable celloxylanase

Gene

xynB

Organism
Clostridium stercorarium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Active toward xylan, carboxymethylcellulose, P-nitrophenyl-beta-D-xylopyranoside and P-nitrophenyl-beta-D-cellobioside.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

pH dependencei

Optimum pH is 7.0.

Temperature dependencei

Optimum temperature is 80 degrees Celsius.

Pathwayi: xylan degradation

This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185Proton donorBy similarity1
Active sitei293NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiGH10. Glycoside Hydrolase Family 10.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermostable celloxylanase (EC:3.2.1.4, EC:3.2.1.8)
Gene namesi
Name:xynB
OrganismiClostridium stercorarium
Taxonomic identifieri1510 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001840611 – 387Thermostable celloxylanaseAdd BLAST387

Structurei

Secondary structure

1387
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 50Combined sources4
Turni51 – 54Combined sources4
Beta strandi57 – 61Combined sources5
Helixi63 – 65Combined sources3
Helixi68 – 77Combined sources10
Beta strandi79 – 85Combined sources7
Helixi89 – 92Combined sources4
Helixi102 – 113Combined sources12
Beta strandi117 – 128Combined sources12
Helixi131 – 134Combined sources4
Beta strandi139 – 141Combined sources3
Helixi142 – 144Combined sources3
Helixi148 – 173Combined sources26
Turni174 – 176Combined sources3
Beta strandi179 – 184Combined sources6
Helixi192 – 194Combined sources3
Helixi199 – 204Combined sources6
Helixi207 – 220Combined sources14
Beta strandi222 – 231Combined sources10
Helixi236 – 251Combined sources16
Beta strandi258 – 261Combined sources4
Beta strandi264 – 268Combined sources5
Helixi272 – 283Combined sources12
Turni284 – 286Combined sources3
Beta strandi288 – 299Combined sources12
Helixi313 – 331Combined sources19
Helixi332 – 336Combined sources5
Beta strandi337 – 343Combined sources7
Helixi351 – 353Combined sources3
Beta strandi355 – 359Combined sources5
Beta strandi364 – 366Combined sources3
Helixi374 – 380Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DEPX-ray1.80A/B41-387[»]
ProteinModelPortaliP40942.
SMRiP40942.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40942.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 382GH10PROSITE-ProRule annotationAdd BLAST342

Sequence similaritiesi

Contains 1 GH10 (glycosyl hydrolase family 10) domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001000. GH10.
IPR031158. GH10_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00591. GH10_1. 1 hit.
PS51760. GH10_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKFLNKKWS LILTMGGIFL MATLSLIFAT GKKAFNDQTS AEDIPSLAEA
60 70 80 90 100
FRDYFPIGAA IEPGYTTGQI AELYKKHVNM LVAENAMKPA SLQPTEGNFQ
110 120 130 140 150
WADADRIVQF AKENGMELRF HTLVWHNQTP TGFSLDKEGK PMVEETDPQK
160 170 180 190 200
REENRKLLLQ RLENYIRAVV LRYKDDIKSW DVVNEVIEPN DPGGMRNSPW
210 220 230 240 250
YQITGTEYIE VAFRATREAG GSDIKLYIND YNTDDPVKRD ILYELVKNLL
260 270 280 290 300
EKGVPIDGVG HQTHIDIYNP PVERIIESIK KFAGLGLDNI ITELDMSIYS
310 320 330 340 350
WNDRSDYGDS IPDYILTLQA KRYQELFDAL KENKDIVSAV VFWGISDKYS
360 370 380
WLNGFPVKRT NAPLLFDRNF MPKPAFWAIV DPSRLRE
Length:387
Mass (Da):44,378
Last modified:February 1, 1995 - v1
Checksum:iC7221BD5E32C8E48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12504 Genomic DNA. Translation: BAA02069.1.
PIRiJC2484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12504 Genomic DNA. Translation: BAA02069.1.
PIRiJC2484.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DEPX-ray1.80A/B41-387[»]
ProteinModelPortaliP40942.
SMRiP40942.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH10. Glycoside Hydrolase Family 10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00114.

Miscellaneous databases

EvolutionaryTraceiP40942.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001000. GH10.
IPR031158. GH10_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00591. GH10_1. 1 hit.
PS51760. GH10_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEXY_CLOSR
AccessioniPrimary (citable) accession number: P40942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.