P40941 (ADT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP,ATP carrier protein 2, mitochondrial

Alternative name(s):
ADP/ATP translocase 2
Adenine nucleotide translocator 2
Short name=ANT 2

Gene names

Name:	AAC2
Synonyms:	ANT2
Ordered Locus Names:	At5g13490
ORF Names:	T6I14_20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Ref.5.
Miscellaneous	The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. At least 2 of the odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue By similarity.
Sequence similarities	Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification] Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transit peptide Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide transport Inferred from direct assay. Source: TAIR
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP:ADP antiporter activity Inferred from direct assay. Source: TAIR copper ion binding Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 74	74	Mitochondrion Potential
	Chain	75 – 385	311	ADP,ATP carrier protein 2, mitochondrial		PRO_0000019246
Regions
	Transmembrane	81 – 115	35	Helical; Name=1; By similarity
	Transmembrane	152 – 176	25	Helical; Name=2; By similarity
	Transmembrane	185 – 219	35	Helical; Name=3; By similarity
	Transmembrane	255 – 282	28	Helical; Name=4; By similarity
	Transmembrane	287 – 321	35	Helical; Name=5; By similarity
	Transmembrane	350 – 375	26	Helical; Name=6; By similarity
	Repeat	82 – 175	94	Solcar 1
	Repeat	187 – 280	94	Solcar 2
	Repeat	288 – 374	87	Solcar 3
	Motif	315 – 320	6	Substrate recognition By similarity
Sites
	Binding site	157	1	Nucleotide By similarity
Experimental info
	Sequence conflict	228	1	G → R in CAA48579. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40941 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 87ADF762213207BA	FASTA	385	41,746
	10 20 30 40 50 60 MVEQTQHPTI LQKVSGQLLS SSVSQDIRGY ASASKRPATY QKHAAYGNYS NAAFQYPLVA 70 80 90 100 110 120 ASQIATTTSP VFVQAPGEKG FTNFAIDFMM GGVSAAVSKT AAAPIERVKL LIQNQDEMLK 130 140 150 160 170 180 AGRLTEPYKG IRDCFGRTIR DEGIGSLWRG NTANVIRYFP TQALNFAFKD YFKRLFNFKK 190 200 210 220 230 240 DKDGYWKWFA GNLASGGAAG ASSLLFVYSL DYARTRLAND SKSAKKGGGE RQFNGLVDVY 250 260 270 280 290 300 KKTLKSDGIA GLYRGFNISC AGIIVYRGLY FGLYDSVKPV LLTGDLQDSF FASFALGWLI 310 320 330 340 350 360 TNGAGLASYP IDTVRRRMMM TSGEAVKYKS SFDAFSQIVK KEGAKSLFKG AGANILRAVA 370 380 GAGVLAGYDK LQLIVFGKKY GSGGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An adenine nucleotide translocator gene from Arabidopsis thaliana." Schuster W., Kloska S., Brennicke A. Biochim. Biophys. Acta 1172:205-208(1993) [PubMed: 8439563] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
[6]	"The growing family of mitochondrial carriers in Arabidopsis." Picault N., Hodges M., Palmieri L., Palmieri F. Trends Plant Sci. 9:138-146(2004) [PubMed: 15003237] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68592 mRNA. Translation: CAA48579.1. AL391710 Genomic DNA. Translation: CAC05426.1. CP002688 Genomic DNA. Translation: AED91901.1. CP002688 Genomic DNA. Translation: AED91902.1. AY050857 mRNA. Translation: AAK92794.1. AY079407 mRNA. Translation: AAL85138.1.
IPI	IPI00537792.
PIR	S29852.
RefSeq	NP_001031876.1. NM_001036799.1. NP_196853.1. NM_121352.3.
UniGene	At.127.
3D structure databases
ProteinModelPortal	P40941.
SMR	P40941. Positions 82-371.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40941. 1 interaction.
STRING	P40941.
Proteomic databases
PRIDE	P40941.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G13490.1; AT5G13490.1; AT5G13490. AT5G13490.2; AT5G13490.2; AT5G13490.
GeneID	831193.
GenomeReviews	Gene locus AT5G13490 in contig BA000015_GR.
KEGG	ath:AT5G13490.
NMPDR	fig|3702.1.peg.23467.
Organism-specific databases
GeneFarm	3732. 374.
TAIR	At5g13490.
Phylogenomic databases
eggNOG	KOG0749.
GeneTree	EPGT00050000009109.
HOGENOM	HBG610399.
InParanoid	P40941.
OMA	YQHEGIN.
PhylomeDB	P40941.
ProtClustDB	CLSN2684949.
Gene expression databases
ArrayExpress	P40941.
Genevestigator	P40941.
GermOnline	AT5G13490. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002113. Aden_trnslctor. IPR002067. Mit_carrier. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Gene3D	G3DSA:1.50.40.10. G3DSA:1.50.40.10. 1 hit.
KO	K05863.
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00927. ADPTRNSLCASE. PR00926. MITOCARRIER.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADT2_ARATH

Accession

Primary (citable) accession number: P40941
Secondary accession number(s): Q9FY52

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	June 6, 2002
Last modified:	November 16, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program