Reviewed, UniProtKB/Swiss-Prot P40938 (RFC3_HUMAN)

Names and origin

Protein names

Recommended name:
    Replication factor C subunit 3
Alternative name(s):
    Activator 1 subunit 3
    Replication factor C 38 kDa subunit
      Short name=RF-C 38 kDa subunit
      Short name=RFC38
    Activator 1 38 kDa subunit
      Short name=A1 38 kDa subunit

Gene names

Name:

RFC3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
Subunit structure	Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.
Subcellular location	NucleusProbable.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the activator 1 small subunits family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA strand elongation during DNA replication Traceable author statement. Source: ProtInc DNA synthesis during DNA repair Traceable author statement. Source: UniProtKB nucleotide-excision repair, DNA gap filling Inferred from Experiment. Source: Reactome
Cellular component	DNA replication factor C complex Inferred from direct assay. Source: UniProtKB nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	DNA clamp loader activity Traceable author statement. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RFC4	P35249	1	EBI-1169853,EBI-476655

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Replication factor C subunit 3		PRO_0000121761
Amino acid modifications
	Modified residue	125	1	Phosphoserine
Natural variations
	Natural variant	16	1	L → V: dbSNP rs3135533.		VAR_018750

Sequences

	Sequence		Length	Mass (Da)
	P40938-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 820C11675A2C63A5	FASTA	356	40,556
	10 20 30 40 50 60 MSLWVDKYRP CSLGRLDYHK EQAAQLRNLV QCGDFPHLLV YGPSGAGKKT RIMCILRELY 70 80 90 100 110 120 GVGVEKLRIE HQTITTPSKK KIEISTIASN YHLEVNPSDA GNSDRVVIQE MLKTVAQSQQ 130 140 150 160 170 180 LETNSQRDFK VVLLTEVDKL TKDAQHALRR TMEKYMSTCR LILCCNSTSK VIPPIRSRCL 190 200 210 220 230 240 AVRVPAPSIE DICHVLSTVC KKEGLNLPSQ LAHRLAEKSC RNLRKALLMC EACRVQQYPF 250 260 270 280 290 300 TADQEIPETD WEVYLRETAN AIVSQQTPQR LLEVRGRLYE LLTHCIPPEI IMKGLLSELL 310 320 330 340 350 HNCDGQLKGE VAQMAAYYEH RLQLGSKAIY HLEAFVAKFM ALYKKFMEDG LEGMMF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homology in accessory proteins of replicative polymerases -- E. coli to humans." O'Donnell M., Onrust R., Dean F.B., Chen M., Hurwitz J. Nucleic Acids Res. 21:1-3(1993) [PubMed: 8441605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Rieder M.J., Braun A.C., Montoya M.A., Chung M.-W., Nguyen C.P., Nguyen D.A., Livingston R.J., Poel C.L., Robertson P.D., Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-16.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9." Rauen M., Burtelow M.A., Dufault V.M., Karnitz L.M. J. Biol. Chem. 275:29767-29771(2000) [PubMed: 10884395] [Abstract] Cited for: INTERACTION WITH RAD17.
[5]	"Purification and characterization of human DNA damage checkpoint Rad complexes." Lindsey-Boltz L.A., Bermudez V.P., Hurwitz J., Sancar A. Proc. Natl. Acad. Sci. U.S.A. 98:11236-11241(2001) [PubMed: 11572977] [Abstract] Cited for: INTERACTION WITH RAD17.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07541 mRNA. Translation: AAB07268.1. AF484446 Genomic DNA. Translation: AAL82505.1. BC000149 mRNA. Translation: AAH00149.1.
PIR	T09573.
RefSeq	NP_002906.1.
UniGene	Hs.115474
3D structure databases
HSSP	HSSP built from PDB template 1IQP based on UniProtKB Q9P9H2.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40938.
PTM databases
PhosphoSite	P40938.
Polymorphism databases
NIEHS-SNPs	Search...
Proteomic databases
PeptideAtlas	P40938.
Genome annotation databases
Ensembl	ENSG00000133119. Homo sapiens. [Contig view]
GeneID	5983.
KEGG	hsa:5983.
Organism-specific databases
H-InvDB	HIX0011228.
HGNC	HGNC:9971. RFC3.
MIM	600405. gene.
PharmGKB	PA34340.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P40938.
HOVERGEN	P40938.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_216. DNA Repair. REACT_383. DNA Replication. REACT_7970. Telomere Maintenance.
Gene expression databases
ArrayExpress	P40938.
CleanEx	HS_RFC3.
GermOnline	ENSG00000133119. Homo sapiens.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase_core. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P40938.
NextBio	23291.
SOURCE	Search...

Entry information

Entry name

RFC3_HUMAN

Accession

Primary (citable) accession number: P40938
Secondary accession number(s): O15252

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 15, 1998
Last modified:	November 25, 2008
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)