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Protein

Replication factor C subunit 5

Gene

RFC5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 67ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • enzyme binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-110312. Translesion synthesis by REV1.
R-HSA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-HSA-110320. Translesion Synthesis by POLH.
R-HSA-174411. Polymerase switching on the C-strand of the telomere.
R-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-HSA-5655862. Translesion synthesis by POLK.
R-HSA-5656121. Translesion synthesis by POLI.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-5685938. HDR through Single Strand Annealing (SSA).
R-HSA-5685942. HDR through Homologous Recombination (HRR).
R-HSA-5693607. Processing of DNA double-strand break ends.
R-HSA-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-69091. Polymerase switching.
R-HSA-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 5
Alternative name(s):
Activator 1 36 kDa subunit
Short name:
A1 36 kDa subunit
Activator 1 subunit 5
Replication factor C 36 kDa subunit
Short name:
RF-C 36 kDa subunit
Short name:
RFC36
Gene namesi
Name:RFC5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111445.13.
HGNCiHGNC:9973. RFC5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5985.
OpenTargetsiENSG00000111445.
PharmGKBiPA34342.

Polymorphism and mutation databases

BioMutaiRFC5.
DMDMi728777.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217511 – 340Replication factor C subunit 5Add BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP40937.
MaxQBiP40937.
PaxDbiP40937.
PeptideAtlasiP40937.
PRIDEiP40937.

PTM databases

iPTMnetiP40937.
PhosphoSitePlusiP40937.

Expressioni

Gene expression databases

BgeeiENSG00000111445.
CleanExiHS_RFC5.
ExpressionAtlasiP40937. baseline and differential.
GenevisibleiP40937. HS.

Organism-specific databases

HPAiHPA041037.

Interactioni

Subunit structurei

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • enzyme binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111917. 66 interactors.
CORUMiP40937.
DIPiDIP-36433N.
IntActiP40937. 52 interactors.
MINTiMINT-1372754.
STRINGi9606.ENSP00000408295.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LFSmodel-A1-340[»]
ProteinModelPortaliP40937.
SMRiP40937.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

eggNOGiKOG0990. Eukaryota.
COG0470. LUCA.
GeneTreeiENSGT00550000075072.
HOGENOMiHOG000224152.
HOVERGENiHBG002053.
InParanoidiP40937.
KOiK10756.
OMAiCTGNPHP.
OrthoDBiEOG091G0CKT.
PhylomeDBiP40937.
TreeFamiTF300810.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P40937-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE
60 70 80 90 100
DRLPHLLLYG PPGTGKTSTI LACAKQLYKD KEFGSMVLEL NASDDRGIDI
110 120 130 140 150
IRGPILSFAS TRTIFKKGFK LVILDEADAM TQDAQNALRR VIEKFTENTR
160 170 180 190 200
FCLICNYLSK IIPALQSRCT RFRFGPLTPE LMVPRLEHVV EEEKVDISED
210 220 230 240 250
GMKALVTLSS GDMRRALNIL QSTNMAFGKV TEETVYTCTG HPLKSDIANI
260 270 280 290 300
LDWMLNQDFT TAYRNITELK TLKGLALHDI LTEIHLFVHR VDFPSSVRIH
310 320 330 340
LLTKMADIEY RLSVGTNEKI QLSSLIAAFQ VTRDLIVAEA
Length:340
Mass (Da):38,497
Last modified:February 1, 1995 - v1
Checksum:iE31E7B8C79933BCE
GO
Isoform 2 (identifier: P40937-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: METSALKQQEQPAATKIRNLPW → M

Note: No experimental confirmation available.
Show »
Length:319
Mass (Da):36,105
Checksum:i6F284482080E75DB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01874913A → T1 PublicationCorresponds to variant dbSNP:rs5745796Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0430671 – 22METSA…RNLPW → M in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07540 mRNA. Translation: AAB09784.1.
AY254323 Genomic DNA. Translation: AAO63493.1.
AK094575 mRNA. Translation: BAG52890.1.
AC131159 Genomic DNA. No translation available.
BC001866 mRNA. Translation: AAH01866.1.
BC013961 mRNA. Translation: AAH13961.1.
CCDSiCCDS41843.2. [P40937-2]
CCDS9185.1. [P40937-1]
RefSeqiNP_001123584.1. NM_001130112.3.
NP_001123585.1. NM_001130113.2. [P40937-2]
NP_031396.1. NM_007370.6. [P40937-1]
NP_853556.2. NM_181578.4. [P40937-2]
XP_011536945.1. XM_011538643.2. [P40937-1]
XP_011536947.1. XM_011538645.2. [P40937-1]
UniGeneiHs.731908.

Genome annotation databases

EnsembliENST00000392542; ENSP00000376325; ENSG00000111445. [P40937-2]
ENST00000454402; ENSP00000408295; ENSG00000111445. [P40937-1]
GeneIDi5985.
KEGGihsa:5985.
UCSCiuc001twq.4. human. [P40937-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRFC5_HUMAN
AccessioniPrimary (citable) accession number: P40937
Secondary accession number(s): A8MZ62, B3KSX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families