P40937 (RFC5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication factor C subunit 5

Alternative name(s):
Activator 1 36 kDa subunit
Short name=A1 36 kDa subunit
Activator 1 subunit 5
Replication factor C 36 kDa subunit
Short name=RF-C 36 kDa subunit
Short name=RFC36

Gene names

Name:

RFC5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Ref.6
Subunit structure	Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA. Ref.6 Ref.7
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the activator 1 small subunits family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA strand elongation involved in DNA replication Traceable author statement. Source: Reactome S phase of mitotic cell cycle Traceable author statement. Source: Reactome cell cycle checkpoint Traceable author statement. Source: Reactome nucleotide-excision repair, DNA gap filling Traceable author statement. Source: Reactome telomere maintenance via recombination Traceable author statement. Source: Reactome telomere maintenance via semi-conservative replication Traceable author statement. Source: Reactome transcription-coupled nucleotide-excision repair Traceable author statement. Source: Reactome
Cellular_component	DNA replication factor C complex Inferred from direct assay PubMed 9488738. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro enzyme binding Non-traceable author statement Ref.6. Source: UniProtKB nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RFC4	P35249	6	EBI-712376,EBI-476655

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P40937-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P40937-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: METSALKQQEQPAATKIRNLPW → M

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 340

340

Replication factor C subunit 5

PRO_0000121751

Regions

Nucleotide binding

60 – 67

8

ATP Potential

Natural variations

Alternative sequence

1 – 22

22

METSA…RNLPW → M in isoform 2.

VSP_043067

Natural variant

13

1

A → T. Ref.2
Corresponds to variant rs5745796 [ dbSNP | Ensembl ].

VAR_018749

Secondary structure

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340

Helix Strand Turn

Details...

Beta strand

6 – 8

3

Helix

13 – 19

7

Helix

22 – 26

5

Turn

31 – 33

3

Helix

38 – 50

13

Beta strand

54 – 60

7

Helix

67 – 87

21

Beta strand

88 – 94

7

Helix

96 – 100

5

Helix

103 – 111

9

Helix

115 – 117

3

Beta strand

121 – 126

6

Helix

128 – 130

3

Helix

133 – 145

13

Turn

146 – 149

4

Beta strand

150 – 155

6

Helix

159 – 161

3

Helix

164 – 168

5

Beta strand

170 – 174

5

Helix

182 – 194

13

Helix

200 – 210

11

Helix

214 – 227

14

Beta strand

229 – 231

3

Helix

233 – 240

8

Helix

245 – 257

13

Helix

260 – 273

14

Helix

278 – 290

13

Beta strand

292 – 294

3

Helix

296 – 313

18

Turn

314 – 316

3

Helix

319 – 334

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: E31E7B8C79933BCE	FASTA	340	38,497
	10 20 30 40 50 60 METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE DRLPHLLLYG 70 80 90 100 110 120 PPGTGKTSTI LACAKQLYKD KEFGSMVLEL NASDDRGIDI IRGPILSFAS TRTIFKKGFK 130 140 150 160 170 180 LVILDEADAM TQDAQNALRR VIEKFTENTR FCLICNYLSK IIPALQSRCT RFRFGPLTPE 190 200 210 220 230 240 LMVPRLEHVV EEEKVDISED GMKALVTLSS GDMRRALNIL QSTNMAFGKV TEETVYTCTG 250 260 270 280 290 300 HPLKSDIANI LDWMLNQDFT TAYRNITELK TLKGLALHDI LTEIHLFVHR VDFPSSVRIH 310 320 330 340 LLTKMADIEY RLSVGTNEKI QLSSLIAAFQ VTRDLIVAEA
	Isoform 2 [UniParc]. Checksum: 6F284482080E75DB	FASTA	319	36,105
	10 20 30 40 50 60 MVEKYRPQTL NDLISHQDIL STIQKFINED RLPHLLLYGP PGTGKTSTIL ACAKQLYKDK 70 80 90 100 110 120 EFGSMVLELN ASDDRGIDII RGPILSFAST RTIFKKGFKL VILDEADAMT QDAQNALRRV 130 140 150 160 170 180 IEKFTENTRF CLICNYLSKI IPALQSRCTR FRFGPLTPEL MVPRLEHVVE EEKVDISEDG 190 200 210 220 230 240 MKALVTLSSG DMRRALNILQ STNMAFGKVT EETVYTCTGH PLKSDIANIL DWMLNQDFTT 250 260 270 280 290 300 AYRNITELKT LKGLALHDIL TEIHLFVHRV DFPSSVRIHL LTKMADIEYR LSVGTNEKIQ 310 LSSLIAAFQV TRDLIVAEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homology in accessory proteins of replicative polymerases -- E. coli to humans." O'Donnell M., Onrust R., Dean F.B., Chen M., Hurwitz J. Nucleic Acids Res. 21:1-3(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	NIEHS SNPs program Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-13.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Amygdala.
[4]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle and Skin.
[6]	"Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen." Mossi R., Jonsson Z.O., Allen B.L., Hardin S.H., Huebscher U. J. Biol. Chem. 272:1769-1776(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PCNA.
[7]	"Purification and characterization of human DNA damage checkpoint Rad complexes." Lindsey-Boltz L.A., Bermudez V.P., Hurwitz J., Sancar A. Proc. Natl. Acad. Sci. U.S.A. 98:11236-11241(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RAD17.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L07540 mRNA. Translation: AAB09784.1.
AY254323 Genomic DNA. Translation: AAO63493.1.
AK094575 mRNA. Translation: BAG52890.1.
AC131159 Genomic DNA. No translation available.
BC001866 mRNA. Translation: AAH01866.1.
BC013961 mRNA. Translation: AAH13961.1.

IPI

IPI00031514.
IPI00925306.

RefSeq

NP_001123584.1. NM_001130112.2.
NP_031396.1. NM_007370.5.
NP_853556.2. NM_181578.3.

UniGene

Hs.731908.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LFS	model	-	A	1-340	[»]

ProteinModelPortal

P40937.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36433N.

IntAct

P40937. 12 interactions.

MINT

MINT-1372754.

STRING

9606.ENSP00000408295.

PTM databases

PhosphoSite

P40937.

Polymorphism databases

DMDM

728777.

Proteomic databases

PaxDb

P40937.

PRIDE

P40937.

Protocols and materials databases

DNASU

5985.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000392542; ENSP00000376325; ENSG00000111445.
ENST00000454402; ENSP00000408295; ENSG00000111445.

GeneID

5985.

KEGG

hsa:5985.

UCSC

uc001twq.3. human.
uc010syx.2. human.

Organism-specific databases

CTD

5985.

GeneCards

GC12P118454.

HGNC

HGNC:9973. RFC5.

HPA

HPA041037.

MIM

600407. gene.

neXtProt

NX_P40937.

PharmGKB

PA34342.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0470.

HOGENOM

HOG000224152.

HOVERGEN

HBG002053.

InParanoid

P40937.

KO

K10756.

OMA

ILACARQ.

PhylomeDB

P40937.

Enzyme and pathway databases

Reactome

REACT_115566. Cell Cycle.
REACT_216. DNA Repair.
REACT_383. DNA Replication.

Gene expression databases

ArrayExpress

P40937.

Bgee

P40937.

CleanEx

HS_RFC5.

Genevestigator

P40937.

GermOnline

ENSG00000111445. Homo sapiens.

Family and domain databases

InterPro

IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view]

Pfam

PF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
[Graphical view]

SUPFAM

SSF48019. Pol_clamp_load_C. 1 hit.
SSF52540. SSF52540. 1 hit.

ProtoNet

Search...

Other

GenomeRNAi

5985.

NextBio

23303.

SOURCE

Search...

Entry information

Entry name

RFC5_HUMAN

Accession

Primary (citable) accession number: P40937
Secondary accession number(s): A8MZ62, B3KSX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.