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Protein

Indolethylamine N-methyltransferase

Gene

Inmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of tryptamine and structurally related compounds (By similarity). Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds.By similarity1 Publication

Caution

Was originally thought to be a thioether S-methyltransferase but appears to be the ortholog of human INMT.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine.1 Publication
S-adenosyl-L-methionine + dimethyl sulfide = S-adenosyl-L-homocysteine + trimethylsulfonium.1 Publication

Enzyme regulationi

Inhibited by the S-adenosyl-L-methionine analog sinefungin and by the product S-adenosyl-L-homocysteine.1 Publication

Kineticsi

  1. KM=0.4 µM for dimethyl selenide
  2. KM=1.0 µM for dimethyl sulfide
  3. KM=1.0 µM for S-adenosyl-L-methionine

    pH dependencei

    Optimum pH is 6.3.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei21S-adenosyl-L-methionineBy similarity1
    Binding sitei26S-adenosyl-L-methionineBy similarity1
    Binding sitei70S-adenosyl-L-methionineBy similarity1
    Binding sitei86S-adenosyl-L-methionineBy similarity1
    Binding sitei91S-adenosyl-L-methionineBy similarity1
    Binding sitei164S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMethyltransferase, Transferase
    Biological processDetoxification
    LigandS-adenosyl-L-methionine

    Enzyme and pathway databases

    BRENDAi2.1.1.96 3474

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Indolethylamine N-methyltransferase (EC:2.1.1.49, EC:2.1.1.96)
    Short name:
    Indolamine N-methyltransferase
    Alternative name(s):
    Aromatic alkylamine N-methyltransferase
    Short name:
    Amine N-methyltransferase
    Short name:
    Arylamine N-methyltransferase
    Thioether S-methyltransferase
    Short name:
    TEMT
    Gene namesi
    Name:Inmt
    Synonyms:Temt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 6

    Organism-specific databases

    MGIiMGI:102963 Inmt

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001597131 – 264Indolethylamine N-methyltransferaseAdd BLAST264

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei14N6-succinyllysineCombined sources1
    Modified residuei97N6-succinyllysineCombined sources1

    Proteomic databases

    MaxQBiP40936
    PaxDbiP40936
    PeptideAtlasiP40936
    PRIDEiP40936

    PTM databases

    iPTMnetiP40936
    PhosphoSitePlusiP40936
    SwissPalmiP40936

    Expressioni

    Tissue specificityi

    Detected in lung and liver (at protein level).1 Publication

    Gene expression databases

    BgeeiENSMUSG00000003477
    CleanExiMM_INMT
    GenevisibleiP40936 MM

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi204112, 1 interactor
    IntActiP40936, 3 interactors
    MINTiP40936
    STRINGi10090.ENSMUSP00000003569

    Structurei

    3D structure databases

    ProteinModelPortaliP40936
    SMRiP40936
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni64 – 65S-adenosyl-L-methionine bindingBy similarity2
    Regioni143 – 144S-adenosyl-L-methionine bindingBy similarity2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410IFTZ Eukaryota
    ENOG41128ZR LUCA
    GeneTreeiENSGT00390000011708
    HOGENOMiHOG000013229
    HOVERGENiHBG000797
    InParanoidiP40936
    KOiK00562
    OMAiYSALCFR
    OrthoDBiEOG091G0EF7
    PhylomeDBiP40936
    TreeFamiTF313114

    Family and domain databases

    InterProiView protein in InterPro
    IPR025820 NNMT/PNMT/TEMT_CS
    IPR000940 NNMT_TEMT_trans
    IPR029063 SAM-dependent_MTases
    PANTHERiPTHR10867 PTHR10867, 1 hit
    PfamiView protein in Pfam
    PF01234 NNMT_PNMT_TEMT, 1 hit
    PIRSFiPIRSF000384 PNMTase, 1 hit
    SUPFAMiSSF53335 SSF53335, 1 hit
    PROSITEiView protein in PROSITE
    PS01100 NNMT_PNMT_TEMT, 1 hit
    PS51681 SAM_MT_NNMT_PNMT_TEMT, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P40936-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEGKVYIGGE DYEKEFTPKD YLTTYYSFHS GPVAEQEIVK FSLQNLYQTF
    60 70 80 90 100
    STGGVGGDVL IDIGSGPTIY QLLSACEVFR EIIVTDYTPQ NLQELQKWLK
    110 120 130 140 150
    KEPGAYDWSS IVQHACELEG DRSRWQEKEA KLRRTVTRVL RCDVTKTPPL
    160 170 180 190 200
    GSAQVPLADC VLTFLAMECA CPDIDTYRAA LRRLAGLLKP GGHLVTLVTL
    210 220 230 240 250
    RFQHYMVGPK KFSGVYLEKE VVEKAIQDAG CQVLKCNCVS LSYSEAYCSH
    260
    DGLCFVVARK GPSA
    Length:264
    Mass (Da):29,460
    Last modified:February 1, 1995 - v1
    Checksum:i58AC5BA580AFB2EE
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti92L → M in BAB28594 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M88694 mRNA Translation: AAA62365.1
    AK002281 mRNA Translation: BAB21985.1
    AK013010 mRNA Translation: BAB28594.1
    BC013518 mRNA Translation: AAH13518.1
    CCDSiCCDS20163.1
    PIRiS52102
    RefSeqiNP_033375.1, NM_009349.3
    UniGeneiMm.299

    Genome annotation databases

    EnsembliENSMUST00000003569; ENSMUSP00000003569; ENSMUSG00000003477
    GeneIDi21743
    KEGGimmu:21743
    UCSCiuc009can.1 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiINMT_MOUSE
    AccessioniPrimary (citable) accession number: P40936
    Secondary accession number(s): Q9CZ50
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: May 23, 2018
    This is version 129 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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