Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phenylethanolamine N-methyltransferase

Gene

Pnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Converts noradrenaline to adrenaline.

Catalytic activityi

S-adenosyl-L-methionine + phenylethanolamine = S-adenosyl-L-homocysteine + N-methylphenylethanolamine.

Pathwayi: (R)-adrenaline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-adrenaline from (R)-noradrenaline.
Proteins known to be involved in this subpathway in this organism are:
  1. Phenylethanolamine N-methyltransferase (Pnmt)
This subpathway is part of the pathway (R)-adrenaline biosynthesis, which is itself part of Catecholamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-adrenaline from (R)-noradrenaline, the pathway (R)-adrenaline biosynthesis and in Catecholamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461S-adenosyl-L-methionineBy similarity
Binding sitei51 – 511S-adenosyl-L-methionineBy similarity
Binding sitei96 – 961S-adenosyl-L-methionineBy similarity
Binding sitei112 – 1121S-adenosyl-L-methionineBy similarity
Binding sitei117 – 1171S-adenosyl-L-methionineBy similarity
Binding sitei192 – 1921S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei230 – 2301SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.28. 3474.
ReactomeiR-MMU-209905. Catecholamine biosynthesis.
UniPathwayiUPA00749; UER00736.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylethanolamine N-methyltransferase (EC:2.1.1.28)
Short name:
PNMTase
Alternative name(s):
Noradrenaline N-methyltransferase
Gene namesi
Name:Pnmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97724. Pnmt.

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4874.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Phenylethanolamine N-methyltransferasePRO_0000159710Add
BLAST

Proteomic databases

PaxDbiP40935.
PRIDEiP40935.

PTM databases

PhosphoSiteiP40935.

Expressioni

Tissue specificityi

Brain (pons and medulla oblongata), adrenal gland and retina.

Gene expression databases

BgeeiP40935.
CleanExiMM_PNMT.
ExpressionAtlasiP40935. baseline and differential.
GenevisibleiP40935. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035549.

Structurei

3D structure databases

ProteinModelPortaliP40935.
SMRiP40935. Positions 37-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati4 – 1071
Repeati14 – 2072

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 20172 X 7 AA repeats of G-S-D-[LP]-K-H-AAdd
BLAST
Regioni90 – 912S-adenosyl-L-methionine bindingBy similarity
Regioni169 – 1702S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IJGY. Eukaryota.
ENOG4111F9R. LUCA.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiP40935.
KOiK00553.
OMAiFAWAQKK.
OrthoDBiEOG7673B9.
PhylomeDBiP40935.
TreeFamiTF313114.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGGSDLKHA TGSGSDPKHA AEMDPDSDAG QVAVALAYQR FEPRAYLRNN
60 70 80 90 100
YAPPRGDLSN PDGVGPWKLR CMAQVFATGE VSGRVLIDIG SGPTIYQLLS
110 120 130 140 150
ACAHFEDITM TDFLEVNRQE LGLWLREEPG AFDWSVYSQH ACLIEDKGES
160 170 180 190 200
WQEKERQLRA RVKRVLPIDV HKPQPLGTPS LVPLPADALV SAFCLEAVSP
210 220 230 240 250
DLTSFQRALH HITTLLRPGG HLLLIGALEE SWYLAGEARL SVVPVSEEEV
260 270 280 290
REALVLGGYE VRELRTYIMP AHLCTGVDDV KGIFFAWAQK MEVQV
Length:295
Mass (Da):32,544
Last modified:February 1, 1995 - v1
Checksum:iED392D6D2244069A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12687 Genomic DNA. Translation: AAA68934.1.
CCDSiCCDS25347.1.
RefSeqiNP_032916.1. NM_008890.1.
UniGeneiMm.57030.

Genome annotation databases

EnsembliENSMUST00000041301; ENSMUSP00000035549; ENSMUSG00000038216.
GeneIDi18948.
KEGGimmu:18948.
UCSCiuc007lgg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12687 Genomic DNA. Translation: AAA68934.1.
CCDSiCCDS25347.1.
RefSeqiNP_032916.1. NM_008890.1.
UniGeneiMm.57030.

3D structure databases

ProteinModelPortaliP40935.
SMRiP40935. Positions 37-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035549.

Chemistry

ChEMBLiCHEMBL4874.

PTM databases

PhosphoSiteiP40935.

Proteomic databases

PaxDbiP40935.
PRIDEiP40935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041301; ENSMUSP00000035549; ENSMUSG00000038216.
GeneIDi18948.
KEGGimmu:18948.
UCSCiuc007lgg.1. mouse.

Organism-specific databases

CTDi5409.
MGIiMGI:97724. Pnmt.

Phylogenomic databases

eggNOGiENOG410IJGY. Eukaryota.
ENOG4111F9R. LUCA.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiP40935.
KOiK00553.
OMAiFAWAQKK.
OrthoDBiEOG7673B9.
PhylomeDBiP40935.
TreeFamiTF313114.

Enzyme and pathway databases

UniPathwayiUPA00749; UER00736.
BRENDAi2.1.1.28. 3474.
ReactomeiR-MMU-209905. Catecholamine biosynthesis.

Miscellaneous databases

NextBioi295288.
PROiP40935.
SOURCEiSearch...

Gene expression databases

BgeeiP40935.
CleanExiMM_PNMT.
ExpressionAtlasiP40935. baseline and differential.
GenevisibleiP40935. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Visualization and ablation of phenylethanolamine N-methyltransferase producing cells in transgenic mice."
    Quaife C.J., Hoyle G.W., Froelick G.J., Findley S.D., Baetge E.E., Behringer R.R., Hammang J.P., Brinster R.L., Palmiter R.D.
    Transgenic Res. 3:388-400(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Organization and complete nucleotide sequence of the gene encoding mouse phenylethanolamine N-methyltransferase."
    Morita S., Kobayashi K., Hidaka H., Nagatsu T.
    Brain Res. Mol. Brain Res. 13:313-319(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBA/2J.

Entry informationi

Entry nameiPNMT_MOUSE
AccessioniPrimary (citable) accession number: P40935
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 16, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.