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Protein

Phenylethanolamine N-methyltransferase

Gene

Pnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Converts noradrenaline to adrenaline.

Catalytic activityi

S-adenosyl-L-methionine + phenylethanolamine = S-adenosyl-L-homocysteine + N-methylphenylethanolamine.

Pathwayi: (R)-adrenaline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-adrenaline from (R)-noradrenaline.
Proteins known to be involved in this subpathway in this organism are:
  1. Phenylethanolamine N-methyltransferase (Pnmt)
This subpathway is part of the pathway (R)-adrenaline biosynthesis, which is itself part of Catecholamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-adrenaline from (R)-noradrenaline, the pathway (R)-adrenaline biosynthesis and in Catecholamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46S-adenosyl-L-methionineBy similarity1
Binding sitei51S-adenosyl-L-methionineBy similarity1
Binding sitei96S-adenosyl-L-methionineBy similarity1
Binding sitei112S-adenosyl-L-methionineBy similarity1
Binding sitei117S-adenosyl-L-methionineBy similarity1
Binding sitei192S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei230SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processCatecholamine biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.28. 3474.
ReactomeiR-MMU-209905. Catecholamine biosynthesis.
UniPathwayiUPA00749; UER00736.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylethanolamine N-methyltransferase (EC:2.1.1.28)
Short name:
PNMTase
Alternative name(s):
Noradrenaline N-methyltransferase
Gene namesi
Name:Pnmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97724. Pnmt.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4874.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597101 – 295Phenylethanolamine N-methyltransferaseAdd BLAST295

Proteomic databases

PaxDbiP40935.
PRIDEiP40935.

PTM databases

iPTMnetiP40935.
PhosphoSitePlusiP40935.

Expressioni

Tissue specificityi

Brain (pons and medulla oblongata), adrenal gland and retina.

Gene expression databases

BgeeiENSMUSG00000038216.
CleanExiMM_PNMT.
ExpressionAtlasiP40935. baseline and differential.
GenevisibleiP40935. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035549.

Structurei

3D structure databases

ProteinModelPortaliP40935.
SMRiP40935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 1017
Repeati14 – 2027

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 202 X 7 AA repeats of G-S-D-[LP]-K-H-AAdd BLAST17
Regioni90 – 91S-adenosyl-L-methionine bindingBy similarity2
Regioni169 – 170S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IJGY. Eukaryota.
ENOG4111F9R. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiP40935.
KOiK00553.
OMAiSACAHFE.
OrthoDBiEOG091G0EF7.
PhylomeDBiP40935.
TreeFamiTF313114.

Family and domain databases

InterProiView protein in InterPro
IPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiView protein in Pfam
PF01234. NNMT_PNMT_TEMT. 1 hit.
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.

Sequencei

Sequence statusi: Complete.

P40935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGGSDLKHA TGSGSDPKHA AEMDPDSDAG QVAVALAYQR FEPRAYLRNN
60 70 80 90 100
YAPPRGDLSN PDGVGPWKLR CMAQVFATGE VSGRVLIDIG SGPTIYQLLS
110 120 130 140 150
ACAHFEDITM TDFLEVNRQE LGLWLREEPG AFDWSVYSQH ACLIEDKGES
160 170 180 190 200
WQEKERQLRA RVKRVLPIDV HKPQPLGTPS LVPLPADALV SAFCLEAVSP
210 220 230 240 250
DLTSFQRALH HITTLLRPGG HLLLIGALEE SWYLAGEARL SVVPVSEEEV
260 270 280 290
REALVLGGYE VRELRTYIMP AHLCTGVDDV KGIFFAWAQK MEVQV
Length:295
Mass (Da):32,544
Last modified:February 1, 1995 - v1
Checksum:iED392D6D2244069A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12687 Genomic DNA. Translation: AAA68934.1.
CCDSiCCDS25347.1.
RefSeqiNP_032916.1. NM_008890.1.
UniGeneiMm.57030.

Genome annotation databases

EnsembliENSMUST00000041301; ENSMUSP00000035549; ENSMUSG00000038216.
GeneIDi18948.
KEGGimmu:18948.
UCSCiuc007lgg.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNMT_MOUSE
AccessioniPrimary (citable) accession number: P40935
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 7, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families