Reviewed, UniProtKB/Swiss-Prot P40935 (PNMT_MOUSE)

Names and origin

Protein names

Recommended name:
    Phenylethanolamine N-methyltransferase
      Short name=PNMTase
    EC=2.1.1.28
Alternative name(s):
    Noradrenaline N-methyltransferase

Gene names

Name:

Pnmt

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts noradrenaline to adrenaline.
Catalytic activity	S-adenosyl-L-methionine + phenylethanolamine = S-adenosyl-L-homocysteine + N-methylphenylethanolamine.
Pathway	Catecholamine biosynthesis; epinephrine biosynthesis; epinephrine from norepinephrine: step 1/1.
Tissue specificity	Brain (pons and medulla oblongata), adrenal gland and retina.
Sequence similarities	Belongs to the NNMT/PNMT/TEMT family.

Ontologies

Keywords
Biological process	Catecholamine biosynthesis
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	catecholamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phenylethanolamine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Phenylethanolamine N-methyltransferase		PRO_0000159710
Regions
	Repeat	4 – 10	7	1
	Repeat	14 – 20	7	2
	Region	4 – 20	17	2 X 7 AA repeats of G-S-D-[LP]-K-H-A
	Region	90 – 91	2	S-adenosyl-L-methionine binding By similarity
	Region	169 – 170	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	46	1	S-adenosyl-L-methionine By similarity
	Binding site	51	1	S-adenosyl-L-methionine By similarity
	Binding site	96	1	S-adenosyl-L-methionine By similarity
	Binding site	112	1	S-adenosyl-L-methionine By similarity
	Binding site	117	1	S-adenosyl-L-methionine By similarity
	Binding site	192	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40935-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: ED392D6D2244069A	FASTA	295	32,544
	10 20 30 40 50 60 MNGGSDLKHA TGSGSDPKHA AEMDPDSDAG QVAVALAYQR FEPRAYLRNN YAPPRGDLSN 70 80 90 100 110 120 PDGVGPWKLR CMAQVFATGE VSGRVLIDIG SGPTIYQLLS ACAHFEDITM TDFLEVNRQE 130 140 150 160 170 180 LGLWLREEPG AFDWSVYSQH ACLIEDKGES WQEKERQLRA RVKRVLPIDV HKPQPLGTPS 190 200 210 220 230 240 LVPLPADALV SAFCLEAVSP DLTSFQRALH HITTLLRPGG HLLLIGALEE SWYLAGEARL 250 260 270 280 290 SVVPVSEEEV REALVLGGYE VRELRTYIMP AHLCTGVDDV KGIFFAWAQK MEVQV

References

[1]	"Visualization and ablation of phenylethanolamine N-methyltransferase producing cells in transgenic mice." Quaife C.J., Hoyle G.W., Froelick G.J., Findley S.D., Baetge E.E., Behringer R.R., Hammang J.P., Brinster R.L., Palmiter R.D. Transgenic Res. 3:388-400(1994) [PubMed: 8000434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Organization and complete nucleotide sequence of the gene encoding mouse phenylethanolamine N-methyltransferase." Morita S., Kobayashi K., Hidaka H., Nagatsu T. Brain Res. Mol. Brain Res. 13:313-319(1992) [PubMed: 1320721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DBA/2J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12687 Genomic DNA. Translation: AAA68934.1.
IPI	IPI00114624.
RefSeq	NP_032916.1.
UniGene	Mm.57030
3D structure databases
HSSP	HSSP built from PDB template 1HNN based on UniProtKB P11086.
SMR	P40935. Positions 25-291.
ModBase	Search...
Proteomic databases
PRIDE	P40935.
Genome annotation databases
Ensembl	ENSMUSG00000038216. Mus musculus. [Contig view]
GeneID	18948.
KEGG	mmu:18948.
Organism-specific databases
MGI	MGI:97724. Pnmt.
Phylogenomic databases
HOGENOM	P40935.
HOVERGEN	P40935.
OMA	P40935. ALEESWY.
Enzyme and pathway databases
BRENDA	2.1.1.28. 244.
Gene expression databases
Bgee	P40935.
CleanEx	MM_PNMT.
GermOnline	ENSMUSG00000038216. Mus musculus.
Family and domain databases
InterPro	IPR000940. NNMT_TEMT_trans. [Graphical view]
PANTHER	PTHR10867. NNMT_TEMT_trans. 1 hit.
Pfam	PF01234. NNMT_PNMT_TEMT. 1 hit. [Graphical view]
PIRSF	PIRSF000384. PNMTase. 1 hit.
PROSITE	PS01100. NNMT_PNMT_TEMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295288.
SOURCE	Search...

Entry information

Entry name

PNMT_MOUSE

Accession

Primary (citable) accession number: P40935

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)