Reviewed, UniProtKB/Swiss-Prot P40934 (CYB5_BRAOB)

Names and origin

Protein names

Recommended name:
    Cytochrome b5

Gene names

Name:

CYB5

Organism

Brassica oleracea var. botrytis (Cauliflower)

Taxonomic identifier

3715 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Cytochrome b5		PRO_0000166023
Regions
	Transmembrane	107 – 127	21	Potential
	Domain	5 – 81	77	Cytochrome b5 heme-binding
Sites
	Metal binding	40	1	Iron (heme axial ligand) By similarity
	Metal binding	64	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	5	1	K → N

Sequences

	Sequence		Length	Mass (Da)
	P40934-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 764DC24A4CDDD591	FASTA	134	15,062
	10 20 30 40 50 60 MASEKKVLGF EEVSQHNKTK DCWLIISGKV YDVTPFMDDH PGGDEVLLSS TGKDATNDFE 70 80 90 100 110 120 DVGHSDTARD MMEKYYIGEI DSSTVPATRT YVAPVQPAYN QDKTPEFMIK ILQFLVPILI 130 LGLALVVRQY TKKE

References

[1]

"Primary structure of cytochrome b5 from cauliflower (Brassica oleracea L.) deduced from peptide and cDNA sequences." Kearns E.V., Keck P., Somerville C.R. Plant Physiol. 99:1254-1257(1992) [PubMed: 16668997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 6-47 AND 75-89. Strain: subvar. cauliflora.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M87514 mRNA. Translation: AAA32990.1.
PIR	T14454.
3D structure databases
HSSP	HSSP built from PDB template 1EHB based on UniProtKB P00171.
ModBase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_BRAOB

Accession

Primary (citable) accession number: P40934

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)