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Reviewed, UniProtKB/Swiss-Prot P40927 (MAOX_COLLI)

Last modified November 25, 2008. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40
Gene names
Name: ME1
OrganismColumba livia (Domestic pigeon)
Taxonomic identifier8932 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Columbiformes › Columbidae › Columba

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Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.

Cofactor

Divalent metal cations. Prefers magnesium or manganese.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the malic enzymes family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 557557NADP-dependent malic enzyme
PRO_0000160198

Regions

Nucleotide binding290 – 30718NADP

Sites

Active site911Proton donor
Active site1621Proton acceptor
Metal binding2341Divalent metal cation
Metal binding2351Divalent metal cation
Metal binding2581Divalent metal cation
Binding site1441NADP
Binding site2581NADP
Binding site3971NADP
Site2581Important for activity
Site3401Confers specificity for NADP

Experimental info

Mutagenesis1411D → N: Increases Km for manganese 14-fold. Increases Km for malate 5-fold
Mutagenesis1621K → A: Decreases Kcat 235-fold. no effect on Km for NADP
Mutagenesis1941D → N: No effect on Km for manganese. Increases Km for malate 8-fold
Mutagenesis3401K → A: Increases Km for NADP 65-fold. No effect on Kcat
Mutagenesis4641D → N: No effect
Sequence conflict2791N → D AA sequence Ref.2

Secondary structure

1 ............................................................................................ 557
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P40927-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7489A2E6741567C8

FASTA55762,054
        10         20         30         40         50         60 
MKKGYEVLRD PHLNKGMAFT LEERQQLNIH GLLPPCFLGQ DAQVYSILKN FERLTSDLDR 

        70         80         90        100        110        120 
YILLMSLQDR NEKLFYKVLT SDIERFMPIV YTPTVGLACQ HYGLAFRRPR GLFITIHDRG 

       130        140        150        160        170        180 
HIATMLQSWP ESVIKAIVVT DGERILGLGD LGCYGMGIPV GKLALYTACG GVKPHQCLPV 

       190        200        210        220        230        240 
MLDVGTDNET LLKDPLYIGL RHKRIRGQAY DDLLDEFMEA VTSRYGMNCL IQFEDFANAN 

       250        260        270        280        290        300 
AFRLLHKYRN KYCTFNDDIQ GTASVAVAGL LAALRITKNR LSDHTVLFQG AGEAALGIAN 

       310        320        330        340        350        360 
LIVMAMQKEG VSKEEAIKRI WMVDSKGLIV KGRASLTPEK EHFAHEHCEM KNLEDIVKDI 

       370        380        390        400        410        420 
KPTVLIGVAA IGGAFTQQIL QDMAAFNKRP IIFALSNPTS KAECTAEQLY KYTEGRGIFA 

       430        440        450        460        470        480 
SGSPFDPVTL PSGQTLYPGQ GNNSYVFPGV ALGVISCGLK HIGDDVFLTT AEVIAQEVSE 

       490        500        510        520        530        540 
ENLQEGRLYP PLVTIQQVSL KIAVRIAKEA YRNNTASTYP QPEDLEAFIR SQVYSTDYNC 

       550 
FVADSYTWPE EAMKVKL

« Hide

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References

[1]"Cloning and expression of pigeon liver cytosolic NADP(+)-dependent malic enzyme cDNA and some of its abortive mutants."
Chou W.-Y., Huang S.-M., Liu Y.H., Chang G.-G.
Arch. Biochem. Biophys. 310:158-166(1994) [PubMed: 8161199] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Affinity cleavage at the putative metal-binding site of pigeon liver malic enzyme by the Fe(2+)-ascorbate system."
Wei C.H., Chou W.-Y., Huang S.-M., Lin C.C., Chang G.-G.
Biochemistry 33:7931-7936(1994) [PubMed: 8011656] [Abstract]
Cited for: PROTEIN SEQUENCE OF 259-283.
[3]"Lysine residues 162 and 340 are involved in the catalysis and coenzyme binding of NADP(+)-dependent malic enzyme from pigeon."
Kuo C.C., Tsai L.C., Chin T.Y., Chang G.G., Chou W.Y.
Biochem. Biophys. Res. Commun. 270:821-825(2000) [PubMed: 10772909] [Abstract]
Cited for: MUTAGENESIS OF LYS-162 AND LYS-340.
[4]"Characterization of the functional role of Asp141, Asp194, and Asp464 residues in the Mn2+-L-malate binding of pigeon liver malic enzyme."
Chou W.Y., Chang H.P., Huang C.H., Kuo C.C., Tong L., Chang G.G.
Protein Sci. 9:242-251(2000) [PubMed: 10716176] [Abstract]
Cited for: MUTAGENESIS OF ASP-141; ASP-194 AND ASP-464.
[5]"Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme."
Yang Z., Zhang H., Huang H.-C., Kuo C.-C., Tsai L.-C., Yuan H.S., Chou W.-Y., Chang G.-G., Tong L.
Protein Sci. 11:332-341(2002) [PubMed: 11790843] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 2-556 IN COMPLEX WITH NADP; MANGANESE AND SUBSTRATE.

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Cross-references

Sequence databases

L09233 mRNA. Translation: AAA49450.1.
PIRS43231.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1GQ2X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P2-556[»]
ModBaseSearch...

Phylogenomic databases

HOVERGENP40927.

Family and domain databases

InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD_bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PRINTSPR00072. MALOXRDTASE.
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMAOX_COLLI
AccessionPrimary (citable) accession number: P40927
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences