Reviewed, UniProtKB/Swiss-Prot P40925 (MDHC_HUMAN)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37
Alternative name(s):
    Cytosolic malate dehydrogenase

Gene names

Name:	MDH1
Synonyms:	MDHA

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.1 Traceable author statement. Source: ProtInc
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro malic enzyme activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 334	333	Malate dehydrogenase, cytoplasmic		PRO_0000113409
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	NAD By similarity
	Binding site	112	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate
Amino acid modifications
	Modified residue	2	1	N-acetylserine
	Modified residue	210	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40925-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 5F7ED9789CA1DB55	FASTA	334	36,426
	10 20 30 40 50 60 MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC 70 80 90 100 110 120 ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA 130 140 150 160 170 180 KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN 190 200 210 220 230 240 VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL 250 260 270 280 290 300 SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV 310 320 330 EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and mapping of a human cDNA for cytosolic malate dehydrogenase (MDH1)." Tanaka T., Inazawa J., Nakamura Y. Genomics 32:128-130(1996) [PubMed: 8786100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	Lo A.S.Y., Waye M.M.Y. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	Bienvenut W.V., Kanor S., Tissot J.-D., Quadroni M. Submitted (MAY-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-18; 205-213 AND 324-334, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: T-cell.
[7]	"The human myocardial two-dimensional gel protein database: update 1994." Corbett J.M., Wheeler C.H., Baker C.S., Yacoub M.H., Dunn M.J. Electrophoresis 15:1459-1465(1994) [PubMed: 7895732] [Abstract] Cited for: PROTEIN SEQUENCE OF 168-181. Tissue: Heart.
[8]	Lubec G., Vishwanath V. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 180-201 AND 299-310, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
+	Additional computationally mapped references.

Web resources

Wikipedia

Malate dehydrogenase entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	D55654 mRNA. Translation: BAA09513.1. U20352 mRNA. Translation: AAC16436.1. CR457405 mRNA. Translation: CAG33686.1. AC016734 Genomic DNA. Translation: AAY14893.1. BC001484 mRNA. Translation: AAH01484.1.
PIR	G01650.
RefSeq	NP_005908.1.
UniGene	Hs.526521
3D structure databases
HSSP	HSSP built from PDB template 4MDH based on UniProtKB P11708.
SMR	P40925. Positions 2-334.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40925.
PTM databases
PhosphoSite	P40925.
2-D gel databases
HSC-2DPAGE	P40925.
REPRODUCTION-2DPAGE	IPI00291005.
Proteomic databases
PeptideAtlas	P40925.
Genome annotation databases
Ensembl	ENSG00000014641. Homo sapiens. [Contig view]
GeneID	4190.
KEGG	hsa:4190.
Organism-specific databases
H-InvDB	HIX0002092.
HGNC	HGNC:6970. MDH1.
MIM	154200. gene.
PharmGKB	PA30714.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P40925.
HOVERGEN	P40925.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13034.
Reactome	REACT_1709. Metabolism of small molecules.
Gene expression databases
ArrayExpress	P40925.
CleanEx	HS_MDH1.
GermOnline	ENSG00000014641. Homo sapiens.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR011274. Malate_DHase_NAD-dep_euk. IPR010945. Malate_DHase_SF1. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00157. NADH.
LinkHub	P40925.
NextBio	16510.
SOURCE	Search...

Entry information

Entry name

MDHC_HUMAN

Accession

Primary (citable) accession number: P40925
Secondary accession number(s): Q6I9V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 91 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)