AP-1-like transcription factor YAP4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40917 (YAP4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
AP-1-like transcription factor YAP4

Alternative name(s):
Chromosome instability protein 5
Transcription activator CIN5

Gene names

Name:	CIN5
Synonyms:	HAL6, SDS15, YAP4
Ordered Locus Names:	YOR028C
ORF Names:	OR26.18

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription activator involved in the regulation of genes expressed in response to environmental changes and metabolic requirements. According to genome-wide promoter binding and gene expression studies it regulates, among others, genes involved in ribosome biogenesis, and protein synthesis. It may also be involved in pleiotropic drug resistance. When overexpressed it confers increased resistance to cisplatin, the DNA-alkylating agents methylmethanosulfonate, and mitomycin C, the antimalarial drugs quinidine, mefloquine, and chloroquine, and increases cellular tolerance to sodium and lithium. Preferentially binds 5'-TTACTAA-3'. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.10.
Miscellaneous	One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities.
Sequence similarities	Belongs to the bZIP family. YAP subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hyperosmotic salinity response Inferred from mutant phenotype Ref.6. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from sequence alignment Ref.7. Source: SGD response to drug Inferred from mutant phenotype Ref.7 PubMed 15821130 Ref.5. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 19029946. Source: SGD nucleus Inferred from direct assay Ref.7 PubMed 19029946. Source: SGD
Molecular_function	repressing transcription factor binding Inferred from direct assay PubMed 21552514. Source: SGD sequence-specific DNA binding Inferred from direct assay PubMed 19111667 PubMed 20802496 Ref.4. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

AP-1-like transcription factor YAP4

PRO_0000076524

Regions

Domain

237 – 295

bZIP

Region

239 – 260

Basic motif By similarity

Region

262 – 271

Leucine-zipper By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

196

Phosphoserine Ref.11

Modified residue

198

Phosphothreonine Ref.11

Sequences

Sequence

Length

Mass (Da)

Tools

P40917 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: D80BF21505267548
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FASTA

295

32,975

        10         20         30         40         50         60 
MLMQIKMDNH PFNFQPILAS HSMTRDSTKP KKMTDTAFVP SPPVGFIKEE NKADLHTISV 

        70         80         90        100        110        120 
VASNVTLPQI QLPKIATLEE PGYESRTGSL TDLSGRRNSV NIGALCEDVP NTAGPHIARP 

       130        140        150        160        170        180 
VTINNLIPPS LPRLNTYQLR PQLSDTHLNC HFNSNPYTTA SHAPFESSYT TASTFTSQPA 

       190        200        210        220        230        240 
ASYFPSNSTP ATRKNSATTN LPSEERRRVS VSLSEQVFNE GERYNNDGQL IGKTGKPLRN 

       250        260        270        280        290 
TKRAAQNRSA QKAFRQRREK YIKNLEEKSK LFDGLMKENS ELKKMIESLK SKLKE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A putative transcriptional activator required for normal microtubule function." Hoyt M. Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions." Fernandes L., Rodrigues-Pousada C., Struhl K. Mol. Cell. Biol. 17:6982-6993(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ISOLATION OF YAP FAMILY PROTEINS.
[5]	"Identification of Saccharomyces cerevisiae genes conferring resistance to quinoline ring-containing antimalarial drugs." Delling U., Raymond M., Schurr E. Antimicrob. Agents Chemother. 42:1034-1041(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RESISTANCE TO ANTIMALARIAL DRUGS.
[6]	"Yeast putative transcription factors involved in salt tolerance." Mendizabal I., Rios G., Mulet J.M., Serrano R., de Larrinoa I.F. FEBS Lett. 425:323-328(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SALT TOLERANCE.
[7]	"Two nuclear proteins, Cin5 and Ydr259c, confer resistance to cisplatin in Saccharomyces cerevisiae." Furuchi T., Ishikawa H., Miura N., Ishizuka M., Kajiya K., Kuge S., Naganuma A. Mol. Pharmacol. 59:470-474(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PLEIOTROPIC DRUG-RESISTANCE.
[8]	"Transcriptional regulatory networks in Saccharomyces cerevisiae." Lee T.I., Rinaldi N.J., Robert F., Odom D.T., Bar-Joseph Z., Gerber G.K., Hannett N.M., Harbison C.T., Thompson C.M., Simon I., Zeitlinger J., Jennings E.G., Murray H.L., Gordon D.B., Ren B., Wyrick J.J., Tagne J.B., Volkert T.L. , Fraenkel E., Gifford D.K., Young R.A. Science 298:799-804(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PROMOTER BINDING.
[9]	"Computational discovery of gene modules and regulatory networks." Bar-Joseph Z., Gerber G.K., Lee T.I., Rinaldi N.J., Yoo J.Y., Robert F., Gordon D.B., Fraenkel E., Jaakkola T.S., Young R.A., Gifford D.K. Nat. Biotechnol. 21:1337-1342(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, REGULATORY TRANSCRIPTION MODULES.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85; SER-89; SER-196 AND THR-198, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16780 Genomic DNA. Translation: AAA52752.1. X87331 Genomic DNA. Translation: CAA60744.1. Z74936 Genomic DNA. Translation: CAA99218.1. BK006948 Genomic DNA. Translation: DAA10810.1.
PIR	S50316.
RefSeq	NP_014671.1. NM_001183447.1.
3D structure databases
ProteinModelPortal	P40917.
SMR	P40917. Positions 243-291.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4351N.
IntAct	P40917. 6 interactions.
MINT	MINT-527155.
STRING	4932.YOR028C.
Proteomic databases
PaxDb	P40917.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR028C; YOR028C; YOR028C.
GeneID	854193.
KEGG	sce:YOR028C.
Organism-specific databases
CYGD	YOR028c.
SGD	S000005554. CIN5.
Phylogenomic databases
eggNOG	NOG76641.
GeneTree	ENSGT00530000068609.
OrthoDB	EOG4TXG2M.
Gene expression databases
Genevestigator	P40917.
GermOnline	YOR028C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004827. bZIP. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. False negative. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976017.

Entry information

Entry name

YAP4_YEAST

Accession

Primary (citable) accession number: P40917
Secondary accession number(s): D6W294

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents