Oligopeptide transporter 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40897 (OPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Oligopeptide transporter 1

Alternative name(s):
High affinity glutathione transporter 1

Gene names

Name:	OPT1
Synonyms:	GSH11, HGT1
Ordered Locus Names:	YJL212C
ORF Names:	J0236, HRD799

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	799 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High affinity transporter for glutathione. Also transports tetra- and pentapeptides like the opioids leucine enkephalin (Tyr-Gly-Gly-Phe-Leu) and methionine enkephalin (Tyr-Gly-Gly_Phe-Met) across the cell membrane. Ref.4 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein.
Miscellaneous	Present with 2870 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the oligopeptide OPT transporter family.
Biophysicochemical properties	Kinetic parameters: K_M=53.9 µM for glutathione Ref.4 Ref.5 K_M=310 µM for leucine enkephalin V_max=10 nmol/min/mg enzyme for glutathione pH dependence: Optimum pH is 5.5.

Ontologies

Keywords
Biological process	Peptide transport Protein transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW sulfur compound metabolic process Inferred from expression pattern. Source: SGD
Cellular component	integral to plasma membrane Inferred from direct assay. Source: SGD
Molecular function	proton-dependent oligopeptide secondary active transmembrane transporter activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 799

799

Oligopeptide transporter 1

PRO_0000213788

Regions

Topological domain

1 – 108

108

Extracellular Potential

Transmembrane

109 – 129

Helical; Potential

Topological domain

130 – 135

Cytoplasmic Potential

Transmembrane

136 – 156

Helical; Potential

Topological domain

157 – 177

Extracellular Potential

Transmembrane

178 – 198

Helical; Potential

Topological domain

199 – 210

Cytoplasmic Potential

Transmembrane

211 – 231

Helical; Potential

Topological domain

232 – 276

Extracellular Potential

Transmembrane

277 – 297

Helical; Potential

Topological domain

298 – 313

Cytoplasmic Potential

Transmembrane

314 – 334

Helical; Potential

Topological domain

335 – 359

Extracellular Potential

Transmembrane

360 – 380

Helical; Potential

Topological domain

381 – 428

Cytoplasmic Potential

Transmembrane

429 – 449

Helical; Potential

Topological domain

450 – 482

Extracellular Potential

Transmembrane

483 – 503

Helical; Potential

Topological domain

504 – 508

Cytoplasmic Potential

Transmembrane

509 – 529

Helical; Potential

Topological domain

530 – 540

Extracellular Potential

Transmembrane

541 – 561

Helical; Potential

Topological domain

562 – 590

Cytoplasmic Potential

Transmembrane

591 – 611

Helical; Potential

Topological domain

612 – 659

Extracellular Potential

Transmembrane

660 – 680

Helical; Potential

Topological domain

681 – 736

Cytoplasmic Potential

Transmembrane

737 – 757

Helical; Potential

Topological domain

758 – 799

Extracellular Potential

Amino acid modifications

Modified residue

Phosphothreonine Ref.8

Modified residue

Phosphothreonine Ref.8

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

640

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P40897 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: AF81676CC9B30759
Show »

FASTA

799

91,616

        10         20         30         40         50         60 
MSTIYRESDS LESEPSPTPT TIPIQINMEE EKKDAFVKNI DEDVNNLTAT TDEEDRDPES 

        70         80         90        100        110        120 
QKFDRHSIQE EGLVWKGDPT YLPNSPYPEV RSAVSIEDDP TIRLNHWRTW FLTTVFVVVF 

       130        140        150        160        170        180 
AGVNQFFSLR YPSLEINFLV AQVVCYPIGR ILALLPDWKC SKVPFFDLNP GPFTKKEHAV 

       190        200        210        220        230        240 
VTIAVALTSS TAYAMYILNA QGSFYNMKLN VGYQFLLVWT SQMIGYGAAG LTRRWVVNPA 

       250        260        270        280        290        300 
SSIWPQTLIS VSLFDSLHSR KVEKTVANGW TMPRYRFFLI VLIGSFIWYW VPGFLFTGLS 

       310        320        330        340        350        360 
YFNVILWGSK TRHNFIANTI FGTQSGLGAL PITFDYTQVS QAMSGSVFAT PFYVSANTYA 

       370        380        390        400        410        420 
SVLIFFVIVL PCLYFTNTWY AKYMPVISGS TYDNTQNKYN VTKILNEDYS INLEKYKEYS 

       430        440        450        460        470        480 
PVFVPFSYLL SYALNFAAVI AVFVHCILYH GKDIVAKFKD RKNGGTDIHM RIYSKNYKDC 

       490        500        510        520        530        540 
PDWWYLLLQI VMIGLGFVAV CCFDTKFPAW AFVIAILISL VNFIPQGILE AMTNQHVGLN 

       550        560        570        580        590        600 
IITELICGYM LPLRPMANLL FKLYGFIVMR QGLNLSRDLK LAMYMKVSPR LIFAVQIYAT 

       610        620        630        640        650        660 
IISGMVNVGV QEWMMHNIDG LCTTDQPNGF TCANGRTVFN ASIIWSLPKY LFSSGRIYNP 

       670        680        690        700        710        720 
LMWFFLIGLL FPLAVYAVQW KFPKFKFAKH IHTPVFFTGP GNIPPSTPYN YSLFFAMSFC 

       730        740        750        760        770        780 
LNLIRKRWRA WFNKYNFVMG AGVEAGVAIS VVIIFLCVQY PGGKLSWWGN NVWKRTYDND 

       790 
YKKFYTLKKG ETFGYDKWW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X." Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:1657-1662(1994) [PubMed: 7725802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Enkephalins are transported by a novel eukaryotic peptide uptake system." Hauser M., Donhardt A.M., Barnes D., Naider F., Becker J.M. J. Biol. Chem. 275:3037-3041(2000) [PubMed: 10652283] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[5]	"Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae." Bourbouloux A., Shahi P., Chakladar A., Delrot S., Bachhawat A.K. J. Biol. Chem. 275:13259-13265(2000) [PubMed: 10788431] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-50 AND THR-51, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z34098 Genomic DNA. Translation: CAA83999.1. Z49487 Genomic DNA. Translation: CAA89509.1. BK006943 Genomic DNA. Translation: DAA08599.1.
PIR	S50773.
RefSeq	NP_012323.1. NM_001181645.1.
3D structure databases
ProteinModelPortal	P40897.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5691N.
MINT	MINT-532906.
STRING	P40897.
Protein family/group databases
TCDB	2.A.67.1.3. oligopeptide transporter (OPT) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL212C; YJL212C; YJL212C.
GeneID	853218.
KEGG	sce:YJL212C.
NMPDR	fig\|4932.3.peg.3286.
Organism-specific databases
CYGD	YJL212c.
SGD	S000003748. OPT1.
Phylogenomic databases
eggNOG	fuNOG07375.
GeneTree	EFGT00050000003548.
HOGENOM	HBG590918.
OMA	IIPGYLF.
OrthoDB	EOG4H49BZ.
Gene expression databases
ArrayExpress	P40897.
Genevestigator	P40897.
GermOnline	YJL212C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004648. Oligpept_transpt. IPR004813. Tetrpept_transpt. [Graphical view]
Pfam	PF03169. OPT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00727. ISP4_OPT. 1 hit. TIGR00728. OPT_sfam. 1 hit.
ProtoNet	Search...
Other
NextBio	973413.

Entry information

Entry name

OPT1_YEAST

Accession

Primary (citable) accession number: P40897
Secondary accession number(s): D6VVY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents