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Protein

Oligopeptide transporter 1

Gene

OPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High affinity transporter for glutathione. Also transports tetra- and pentapeptides like the opioids leucine enkephalin (Tyr-Gly-Gly-Phe-Leu) and methionine enkephalin (Tyr-Gly-Gly_Phe-Met) across the cell membrane.2 Publications

Kineticsi

  1. KM=53.9 µM for glutathione2 Publications
  2. KM=310 µM for leucine enkephalin2 Publications
  1. Vmax=10 nmol/min/mg enzyme for glutathione2 Publications

pH dependencei

Optimum pH is 5.5.2 Publications

GO - Molecular functioni

  • oligopeptide transporter activity Source: SGD
  • proton-dependent oligopeptide secondary active transmembrane transporter activity Source: SGD

GO - Biological processi

  • oligopeptide transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • sulfur compound metabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31639-MONOMER.

Protein family/group databases

TCDBi2.A.67.1.3. the oligopeptide transporter (opt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligopeptide transporter 1
Alternative name(s):
High affinity glutathione transporter 1
Gene namesi
Name:OPT1
Synonyms:GSH11, HGT1
Ordered Locus Names:YJL212C
ORF Names:HRD799, J0236
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL212C.
SGDiS000003748. OPT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 108108ExtracellularSequence analysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence analysisAdd
BLAST
Topological domaini130 – 1356CytoplasmicSequence analysis
Transmembranei136 – 15621HelicalSequence analysisAdd
BLAST
Topological domaini157 – 17721ExtracellularSequence analysisAdd
BLAST
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Topological domaini199 – 21012CytoplasmicSequence analysisAdd
BLAST
Transmembranei211 – 23121HelicalSequence analysisAdd
BLAST
Topological domaini232 – 27645ExtracellularSequence analysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence analysisAdd
BLAST
Topological domaini298 – 31316CytoplasmicSequence analysisAdd
BLAST
Transmembranei314 – 33421HelicalSequence analysisAdd
BLAST
Topological domaini335 – 35925ExtracellularSequence analysisAdd
BLAST
Transmembranei360 – 38021HelicalSequence analysisAdd
BLAST
Topological domaini381 – 42848CytoplasmicSequence analysisAdd
BLAST
Transmembranei429 – 44921HelicalSequence analysisAdd
BLAST
Topological domaini450 – 48233ExtracellularSequence analysisAdd
BLAST
Transmembranei483 – 50321HelicalSequence analysisAdd
BLAST
Topological domaini504 – 5085CytoplasmicSequence analysis
Transmembranei509 – 52921HelicalSequence analysisAdd
BLAST
Topological domaini530 – 54011ExtracellularSequence analysisAdd
BLAST
Transmembranei541 – 56121HelicalSequence analysisAdd
BLAST
Topological domaini562 – 59029CytoplasmicSequence analysisAdd
BLAST
Transmembranei591 – 61121HelicalSequence analysisAdd
BLAST
Topological domaini612 – 65948ExtracellularSequence analysisAdd
BLAST
Transmembranei660 – 68021HelicalSequence analysisAdd
BLAST
Topological domaini681 – 73656CytoplasmicSequence analysisAdd
BLAST
Transmembranei737 – 75721HelicalSequence analysisAdd
BLAST
Topological domaini758 – 79942ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 799799Oligopeptide transporter 1PRO_0000213788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence analysis
Modified residuei48 – 481PhosphothreonineCombined sources
Modified residuei50 – 501PhosphothreonineCombined sources
Modified residuei51 – 511PhosphothreonineCombined sources
Glycosylationi640 – 6401N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP40897.

PTM databases

iPTMnetiP40897.

Interactioni

Protein-protein interaction databases

BioGridi33547. 35 interactions.
DIPiDIP-5691N.
MINTiMINT-532906.

Structurei

3D structure databases

ProteinModelPortaliP40897.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000068053.
HOGENOMiHOG000166970.
InParanoidiP40897.
OMAiNEDYSIN.
OrthoDBiEOG70KGZ1.

Family and domain databases

InterProiIPR004648. Oligpept_transpt.
IPR004813. OPT.
[Graphical view]
PfamiPF03169. OPT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00727. ISP4_OPT. 1 hit.
TIGR00728. OPT_sfam. 1 hit.

Sequencei

Sequence statusi: Complete.

P40897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIYRESDS LESEPSPTPT TIPIQINMEE EKKDAFVKNI DEDVNNLTAT
60 70 80 90 100
TDEEDRDPES QKFDRHSIQE EGLVWKGDPT YLPNSPYPEV RSAVSIEDDP
110 120 130 140 150
TIRLNHWRTW FLTTVFVVVF AGVNQFFSLR YPSLEINFLV AQVVCYPIGR
160 170 180 190 200
ILALLPDWKC SKVPFFDLNP GPFTKKEHAV VTIAVALTSS TAYAMYILNA
210 220 230 240 250
QGSFYNMKLN VGYQFLLVWT SQMIGYGAAG LTRRWVVNPA SSIWPQTLIS
260 270 280 290 300
VSLFDSLHSR KVEKTVANGW TMPRYRFFLI VLIGSFIWYW VPGFLFTGLS
310 320 330 340 350
YFNVILWGSK TRHNFIANTI FGTQSGLGAL PITFDYTQVS QAMSGSVFAT
360 370 380 390 400
PFYVSANTYA SVLIFFVIVL PCLYFTNTWY AKYMPVISGS TYDNTQNKYN
410 420 430 440 450
VTKILNEDYS INLEKYKEYS PVFVPFSYLL SYALNFAAVI AVFVHCILYH
460 470 480 490 500
GKDIVAKFKD RKNGGTDIHM RIYSKNYKDC PDWWYLLLQI VMIGLGFVAV
510 520 530 540 550
CCFDTKFPAW AFVIAILISL VNFIPQGILE AMTNQHVGLN IITELICGYM
560 570 580 590 600
LPLRPMANLL FKLYGFIVMR QGLNLSRDLK LAMYMKVSPR LIFAVQIYAT
610 620 630 640 650
IISGMVNVGV QEWMMHNIDG LCTTDQPNGF TCANGRTVFN ASIIWSLPKY
660 670 680 690 700
LFSSGRIYNP LMWFFLIGLL FPLAVYAVQW KFPKFKFAKH IHTPVFFTGP
710 720 730 740 750
GNIPPSTPYN YSLFFAMSFC LNLIRKRWRA WFNKYNFVMG AGVEAGVAIS
760 770 780 790
VVIIFLCVQY PGGKLSWWGN NVWKRTYDND YKKFYTLKKG ETFGYDKWW
Length:799
Mass (Da):91,616
Last modified:February 1, 1995 - v1
Checksum:iAF81676CC9B30759
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83999.1.
Z49487 Genomic DNA. Translation: CAA89509.1.
BK006943 Genomic DNA. Translation: DAA08599.1.
PIRiS50773.
RefSeqiNP_012323.1. NM_001181645.1.

Genome annotation databases

EnsemblFungiiYJL212C; YJL212C; YJL212C.
GeneIDi853218.
KEGGisce:YJL212C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83999.1.
Z49487 Genomic DNA. Translation: CAA89509.1.
BK006943 Genomic DNA. Translation: DAA08599.1.
PIRiS50773.
RefSeqiNP_012323.1. NM_001181645.1.

3D structure databases

ProteinModelPortaliP40897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33547. 35 interactions.
DIPiDIP-5691N.
MINTiMINT-532906.

Protein family/group databases

TCDBi2.A.67.1.3. the oligopeptide transporter (opt) family.

PTM databases

iPTMnetiP40897.

Proteomic databases

MaxQBiP40897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL212C; YJL212C; YJL212C.
GeneIDi853218.
KEGGisce:YJL212C.

Organism-specific databases

EuPathDBiFungiDB:YJL212C.
SGDiS000003748. OPT1.

Phylogenomic databases

GeneTreeiENSGT00530000068053.
HOGENOMiHOG000166970.
InParanoidiP40897.
OMAiNEDYSIN.
OrthoDBiEOG70KGZ1.

Enzyme and pathway databases

BioCyciYEAST:G3O-31639-MONOMER.

Miscellaneous databases

PROiP40897.

Family and domain databases

InterProiIPR004648. Oligpept_transpt.
IPR004813. OPT.
[Graphical view]
PfamiPF03169. OPT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00727. ISP4_OPT. 1 hit.
TIGR00728. OPT_sfam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
    Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Enkephalins are transported by a novel eukaryotic peptide uptake system."
    Hauser M., Donhardt A.M., Barnes D., Naider F., Becker J.M.
    J. Biol. Chem. 275:3037-3041(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  5. "Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae."
    Bourbouloux A., Shahi P., Chakladar A., Delrot S., Bachhawat A.K.
    J. Biol. Chem. 275:13259-13265(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-48; THR-50 AND THR-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOPT1_YEAST
AccessioniPrimary (citable) accession number: P40897
Secondary accession number(s): D6VVY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.