P40890 (VTH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
VPS10 homolog 2

Alternative name(s):
Sortilin VTH2

Gene names

Name:	VTH2
Ordered Locus Names:	YJL222W
ORF Names:	J0213, HRC1549

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. Ref.4 Ref.5
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Miscellaneous	Present with 279 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the VPS10-related sortilin family. Contains 12 BNR repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Nucleotide-binding
Molecular function	Receptor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Inferred from genetic interaction Ref.4. Source: SGD protein targeting to vacuole Inferred from genetic interaction Ref.5. Source: SGD
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Inferred from electronic annotation. Source: UniProtKB-KW signal sequence binding Inferred from genetic interaction Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YHR080C	P38800	1	EBI-26271,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 1549	1528	VPS10 homolog 2		PRO_0000014331
Regions
	Topological domain	22 – 1369	1348	Lumenal Potential
	Transmembrane	1370 – 1390	21	Helical; Potential
	Topological domain	1391 – 1549	159	Cytoplasmic Potential
	Repeat	57 – 68	12	BNR 1
	Repeat	101 – 112	12	BNR 2
	Repeat	159 – 170	12	BNR 3
	Repeat	228 – 239	12	BNR 4
	Repeat	393 – 404	12	BNR 5
	Repeat	465 – 476	12	BNR 6
	Repeat	511 – 522	12	BNR 7
	Repeat	740 – 751	12	BNR 8
	Repeat	837 – 848	12	BNR 9
	Repeat	1040 – 1051	12	BNR 10
	Repeat	1119 – 1130	12	BNR 11
	Repeat	1160 – 1171	12	BNR 12
Amino acid modifications
	Glycosylation	479	1	N-linked (GlcNAc...) Potential
	Glycosylation	769	1	N-linked (GlcNAc...) Potential
	Glycosylation	986	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P40890 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: B260CF7C927B4E4F	FASTA	1,549	174,402
	10 20 30 40 50 60 MALFRALYII WVFLLIPLSN AEEFTPKVTR TLSRYVFDIV NFDDSNTLIR AEEDSVEISF 70 80 90 100 110 120 DAGENWKTID EIEEPIESFV VDPFRGHDRA FAFVKTAPKF YVTDDQGKSW RPLTIPISEK 130 140 150 160 170 180 ASNYFCDVTT HPIKKKHLII RCDLLTIKNS GLMYVGREIY TTNDGVSFSQ VKPSFGKIDG 190 200 210 220 230 240 HISTARCDFI KSSEDSDLGG NDASILCLFR NTEYIESTGS TIDKSELILS ADGGETFKEL 250 260 270 280 290 300 VQFKDKVVSR YEILKHHVIV LTQDDMYNEM SSTNIWISND VSTFQVARTP TKIRHVNMGQ 310 320 330 340 350 360 IHEDSIGRIV LPVSRERDDE DSNQPGAAEV LISDSEGLKF LPINWIPNNQ FGYINVAYPG 370 380 390 400 410 420 FLKGTFFGSF HPFIEYSDRK RKYSRQKVRE ETKVSVDNGL TWTNLKVVDR ENVDLFGCDV 430 440 450 460 470 480 TKPERCSLQT HFYDLRNLNP SAGIMMISGI VGDGSAYNWK EEKTFISRDS GLTWRLVHNS 490 500 510 520 530 540 TGLYTTGDLG NIIMYIPYRS NENGDVPSKF YYSLDQGKTW GEYDLIMPIY PYRLVSTISD 550 560 570 580 590 600 GSGSKFILTG TSITEDPIFI TYSIDFSAVF DYKSCEEGDF EDWNLADGKC VNGAKYKYRR 610 620 630 640 650 660 RKQDAQCLVK KAFKDLSLDE TPCNSCTGSD YECSFEFVRD AKGDCIPDYN LIALSDICDK 670 680 690 700 710 720 SKGKSVLVKP LQLIKGDKCK TPMKIESVDI PCDEIPKEGS SDKEIVTTEN KFDFEIKFYQ 730 740 750 760 770 780 YFDTVADESL VMLNSIGDAY ISHDGGQTIK RFDTDGEKIV EIVFNPYFNS SAYLFGSKGN 790 800 810 820 830 840 IFLTHDRGYS FMIAKLPEAR QLGMPLDFSA KAQDTFIYYG GKNCESILSP ECHAVAYLTK 850 860 870 880 890 900 DGGETFTEML DNAIHCEFAG TLFKYPSNDD MVMCQVKEKF SQTRSLVSST DFFQDDRKTV 910 920 930 940 950 960 FENIIGYLST GGYIIVAVPH EDNELRAYVT NDGAEFTEAK FPYDEDIGKQ DAFTILGSEE 970 980 990 1000 1010 1020 GSIFLHLATN LESGHDFGNL LKSNSNGTSF VTLEHAVNRN TFGYVDFEKV QGLEGIIITN 1030 1040 1050 1060 1070 1080 IVSNSEKVGE NKEDEQLKTK ITFNDGSDWN FLKPPKKDSE GKKFPCDSVS LDKCSLHLHG 1090 1100 1110 1120 1130 1140 YTERKDIRDT YSSGSALGMM FGVGNVGDRL LPYEECSTFL TTDGGETWTE VKKGPHQWEY 1150 1160 1170 1180 1190 1200 GDHGGVLVLV PENAETDSIS YSTDFGKTWK DYKFCGDKVL VKDIITVPRD SALRFLLFGE 1210 1220 1230 1240 1250 1260 AKNMGSGSFR TYTIDFRNIF ERQCEFDITG RKRADFKYSP LGSRTGCLFG HKTEFLRKTD 1270 1280 1290 1300 1310 1320 EKCFIGNIPL SEFSRNVKNC PCTRQDFECD YNFYKASDGT CKLVKGLSSA NGADICKKEP 1330 1340 1350 1360 1370 1380 DLIEYYDSSG YRKIPLSTCK GGLKLDAHLA PHPCPGKEKA FREKYSINTG AYALVFVTIL 1390 1400 1410 1420 1430 1440 LVIFFVAWFV YDRGIRRNGG FSRFEEIRLG DDGLIENNRT DRVVNIIVRL GLCISLITKS 1450 1460 1470 1480 1490 1500 AFQRAKAGTA QLSSKFRARF GNKKGATYSS LLHDQLSDEP DGFHEDSNDL SSFRGQGSNS 1510 1520 1530 1540 EIEQEDVDTS QQEHTSRTDL LGASNIPDAL PARSASHESD LAAARSEDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X." Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:1657-1662(1994) [PubMed: 7725802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Multiple pathways for vacuolar sorting of yeast proteinase A." Westphal V., Marcusson E.G., Winther J.R., Emr S.D., van den Hazel H.B. J. Biol. Chem. 271:11865-11870(1996) [PubMed: 8662642] [Abstract] Cited for: FUNCTION.
[5]	"Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases." Cooper A.A., Stevens T.H. J. Cell Biol. 133:529-541(1996) [PubMed: 8636229] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z34098 Genomic DNA. Translation: CAA83988.1. Z49497 Genomic DNA. Translation: CAA89519.1. BK006943 Genomic DNA. Translation: DAA08591.1.
PIR	S50705.
RefSeq	NP_012313.1. NM_001181655.1.
3D structure databases
ProteinModelPortal	P40890.
SMR	P40890. Positions 44-113, 736-795.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6665N.
IntAct	P40890. 2 interactions.
MINT	MINT-636859.
STRING	P40890.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL222W; YJL222W; YJL222W.
GeneID	853233.
KEGG	sce:YJL222W.
Organism-specific databases
CYGD	YJL222w.
SGD	S000003758. VTH2.
Phylogenomic databases
eggNOG	fuNOG04761.
GeneTree	EFGT00050000004688.
HOGENOM	HBG395571.
OrthoDB	EOG46T68P.
Gene expression databases
Genevestigator	P40890.
GermOnline	YJL222W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002860. BNR_rpt. IPR011040. Neuraminidase. IPR006581. VPS10. [Graphical view]
Gene3D	G3DSA:2.120.10.10. Neuraminidase. 4 hits.
Pfam	PF02012. BNR. 10 hits. [Graphical view]
SMART	SM00602. VPS10. 2 hits. [Graphical view]
SUPFAM	SSF50939. Sialidase. 2 hits.
ProtoNet	Search...
Other
NextBio	973452.

Entry information

Entry name

VTH2_YEAST

Accession

Primary (citable) accession number: P40890
Secondary accession number(s): D6VVX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program