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Protein

VPS10 homolog 2

Gene

VTH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A.2 Publications

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. signal sequence binding Source: SGD

GO - Biological processi

  1. Golgi to vacuole transport Source: SGD
  2. protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31644-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
VPS10 homolog 2
Alternative name(s):
Sortilin VTH2
Gene namesi
Name:VTH2
Ordered Locus Names:YJL222W
ORF Names:HRC1549, J0213
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL222w.
SGDiS000003758. VTH2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 13691348LumenalSequence AnalysisAdd
BLAST
Transmembranei1370 – 139021HelicalSequence AnalysisAdd
BLAST
Topological domaini1391 – 1549159CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 15491528VPS10 homolog 2PRO_0000014331Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi479 – 4791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi769 – 7691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi986 – 9861N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP40890.
PaxDbiP40890.

Expressioni

Gene expression databases

GenevestigatoriP40890.

Interactioni

Protein-protein interaction databases

BioGridi33560. 12 interactions.
DIPiDIP-6665N.
IntActiP40890. 1 interaction.
MINTiMINT-636859.
STRINGi4932.YJL222W.

Structurei

3D structure databases

ProteinModelPortaliP40890.
SMRiP40890. Positions 509-541, 898-1343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati57 – 6812BNR 1Add
BLAST
Repeati101 – 11212BNR 2Add
BLAST
Repeati159 – 17012BNR 3Add
BLAST
Repeati228 – 23912BNR 4Add
BLAST
Repeati393 – 40412BNR 5Add
BLAST
Repeati465 – 47612BNR 6Add
BLAST
Repeati511 – 52212BNR 7Add
BLAST
Repeati740 – 75112BNR 8Add
BLAST
Repeati837 – 84812BNR 9Add
BLAST
Repeati1040 – 105112BNR 10Add
BLAST
Repeati1119 – 113012BNR 11Add
BLAST
Repeati1160 – 117112BNR 12Add
BLAST

Sequence similaritiesi

Belongs to the VPS10-related sortilin family.Curated
Contains 12 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG281049.
GeneTreeiENSGT00510000046443.
HOGENOMiHOG000169063.
InParanoidiP40890.
OrthoDBiEOG7DVDKD.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR011040. Sialidases.
IPR006581. VPS10.
[Graphical view]
SMARTiSM00602. VPS10. 2 hits.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40890-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALFRALYII WVFLLIPLSN AEEFTPKVTR TLSRYVFDIV NFDDSNTLIR
60 70 80 90 100
AEEDSVEISF DAGENWKTID EIEEPIESFV VDPFRGHDRA FAFVKTAPKF
110 120 130 140 150
YVTDDQGKSW RPLTIPISEK ASNYFCDVTT HPIKKKHLII RCDLLTIKNS
160 170 180 190 200
GLMYVGREIY TTNDGVSFSQ VKPSFGKIDG HISTARCDFI KSSEDSDLGG
210 220 230 240 250
NDASILCLFR NTEYIESTGS TIDKSELILS ADGGETFKEL VQFKDKVVSR
260 270 280 290 300
YEILKHHVIV LTQDDMYNEM SSTNIWISND VSTFQVARTP TKIRHVNMGQ
310 320 330 340 350
IHEDSIGRIV LPVSRERDDE DSNQPGAAEV LISDSEGLKF LPINWIPNNQ
360 370 380 390 400
FGYINVAYPG FLKGTFFGSF HPFIEYSDRK RKYSRQKVRE ETKVSVDNGL
410 420 430 440 450
TWTNLKVVDR ENVDLFGCDV TKPERCSLQT HFYDLRNLNP SAGIMMISGI
460 470 480 490 500
VGDGSAYNWK EEKTFISRDS GLTWRLVHNS TGLYTTGDLG NIIMYIPYRS
510 520 530 540 550
NENGDVPSKF YYSLDQGKTW GEYDLIMPIY PYRLVSTISD GSGSKFILTG
560 570 580 590 600
TSITEDPIFI TYSIDFSAVF DYKSCEEGDF EDWNLADGKC VNGAKYKYRR
610 620 630 640 650
RKQDAQCLVK KAFKDLSLDE TPCNSCTGSD YECSFEFVRD AKGDCIPDYN
660 670 680 690 700
LIALSDICDK SKGKSVLVKP LQLIKGDKCK TPMKIESVDI PCDEIPKEGS
710 720 730 740 750
SDKEIVTTEN KFDFEIKFYQ YFDTVADESL VMLNSIGDAY ISHDGGQTIK
760 770 780 790 800
RFDTDGEKIV EIVFNPYFNS SAYLFGSKGN IFLTHDRGYS FMIAKLPEAR
810 820 830 840 850
QLGMPLDFSA KAQDTFIYYG GKNCESILSP ECHAVAYLTK DGGETFTEML
860 870 880 890 900
DNAIHCEFAG TLFKYPSNDD MVMCQVKEKF SQTRSLVSST DFFQDDRKTV
910 920 930 940 950
FENIIGYLST GGYIIVAVPH EDNELRAYVT NDGAEFTEAK FPYDEDIGKQ
960 970 980 990 1000
DAFTILGSEE GSIFLHLATN LESGHDFGNL LKSNSNGTSF VTLEHAVNRN
1010 1020 1030 1040 1050
TFGYVDFEKV QGLEGIIITN IVSNSEKVGE NKEDEQLKTK ITFNDGSDWN
1060 1070 1080 1090 1100
FLKPPKKDSE GKKFPCDSVS LDKCSLHLHG YTERKDIRDT YSSGSALGMM
1110 1120 1130 1140 1150
FGVGNVGDRL LPYEECSTFL TTDGGETWTE VKKGPHQWEY GDHGGVLVLV
1160 1170 1180 1190 1200
PENAETDSIS YSTDFGKTWK DYKFCGDKVL VKDIITVPRD SALRFLLFGE
1210 1220 1230 1240 1250
AKNMGSGSFR TYTIDFRNIF ERQCEFDITG RKRADFKYSP LGSRTGCLFG
1260 1270 1280 1290 1300
HKTEFLRKTD EKCFIGNIPL SEFSRNVKNC PCTRQDFECD YNFYKASDGT
1310 1320 1330 1340 1350
CKLVKGLSSA NGADICKKEP DLIEYYDSSG YRKIPLSTCK GGLKLDAHLA
1360 1370 1380 1390 1400
PHPCPGKEKA FREKYSINTG AYALVFVTIL LVIFFVAWFV YDRGIRRNGG
1410 1420 1430 1440 1450
FSRFEEIRLG DDGLIENNRT DRVVNIIVRL GLCISLITKS AFQRAKAGTA
1460 1470 1480 1490 1500
QLSSKFRARF GNKKGATYSS LLHDQLSDEP DGFHEDSNDL SSFRGQGSNS
1510 1520 1530 1540
EIEQEDVDTS QQEHTSRTDL LGASNIPDAL PARSASHESD LAAARSEDK
Length:1,549
Mass (Da):174,402
Last modified:February 1, 1995 - v1
Checksum:iB260CF7C927B4E4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83988.1.
Z49497 Genomic DNA. Translation: CAA89519.1.
BK006943 Genomic DNA. Translation: DAA08591.1.
PIRiS50705.
RefSeqiNP_012313.1. NM_001181655.1.

Genome annotation databases

EnsemblFungiiYJL222W; YJL222W; YJL222W.
GeneIDi853233.
KEGGisce:YJL222W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83988.1.
Z49497 Genomic DNA. Translation: CAA89519.1.
BK006943 Genomic DNA. Translation: DAA08591.1.
PIRiS50705.
RefSeqiNP_012313.1. NM_001181655.1.

3D structure databases

ProteinModelPortaliP40890.
SMRiP40890. Positions 509-541, 898-1343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33560. 12 interactions.
DIPiDIP-6665N.
IntActiP40890. 1 interaction.
MINTiMINT-636859.
STRINGi4932.YJL222W.

Proteomic databases

MaxQBiP40890.
PaxDbiP40890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL222W; YJL222W; YJL222W.
GeneIDi853233.
KEGGisce:YJL222W.

Organism-specific databases

CYGDiYJL222w.
SGDiS000003758. VTH2.

Phylogenomic databases

eggNOGiNOG281049.
GeneTreeiENSGT00510000046443.
HOGENOMiHOG000169063.
InParanoidiP40890.
OrthoDBiEOG7DVDKD.

Enzyme and pathway databases

BioCyciYEAST:G3O-31644-MONOMER.

Miscellaneous databases

NextBioi973452.

Gene expression databases

GenevestigatoriP40890.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR011040. Sialidases.
IPR006581. VPS10.
[Graphical view]
SMARTiSM00602. VPS10. 2 hits.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
    Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION.
  5. "Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases."
    Cooper A.A., Stevens T.H.
    J. Cell Biol. 133:529-541(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVTH2_YEAST
AccessioniPrimary (citable) accession number: P40890
Secondary accession number(s): D6VVX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 4, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.