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P40884

- IMA5_YEAST

UniProt

P40884 - IMA5_YEAST

Protein

Oligo-1,6-glucosidase IMA5

Gene

IMA5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Alpha-glucosidase with specificity for isomaltose, maltose, and palatinose.1 Publication

    Catalytic activityi

    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei210 – 2101NucleophileBy similarity
    Active sitei272 – 2721Proton donorBy similarity
    Sitei347 – 3471Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. oligo-1,6-glucosidase activity Source: SGD

    GO - Biological processi

    1. disaccharide catabolic process Source: SGD
    2. maltose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Maltose metabolism

    Enzyme and pathway databases

    BioCyciYEAST:YJL216C-MONOMER.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oligo-1,6-glucosidase IMA5 (EC:3.2.1.10)
    Alternative name(s):
    Alpha-glucosidase
    Isomaltase 5
    Gene namesi
    Name:IMA5
    Ordered Locus Names:YJL216C
    ORF Names:HRF581, J0228
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL216c.
    SGDiS000003752. IMA5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 581581Oligo-1,6-glucosidase IMA5PRO_0000054331Add
    BLAST

    Expressioni

    Inductioni

    Transcriptionally regulated by PDR8. Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside.2 Publications

    Gene expression databases

    GenevestigatoriP40884.

    Interactioni

    Protein-protein interaction databases

    BioGridi33544. 6 interactions.
    STRINGi4932.YJL216C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40884.
    SMRiP40884. Positions 7-579.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiCOG0366.
    GeneTreeiENSGT00530000063127.
    KOiK01187.
    OMAiHETESEW.
    OrthoDBiEOG7M3J7X.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40884-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIIHNPKWW KEATVYQIYP ASFKDSNNDG WGDLAGITSK LDYVKELGVD    50
    AIWVCPFYDS PQEDMGYDIA NYEKVWPRYG TNEDCFQMIE EAHKRGIKVI 100
    VDLVINHCSE EHEWFKESRS SKANPKRDWF FWRPPKGYDE KGNPIPPNNW 150
    RSFFGGSAWR YDEKTGEFFL HVFALGQPDF NWENEECRKA IYDSSVGYWL 200
    RHNVDGFRID VGSMYSKVEG LPDAPITDPT VPYQKGTEFF INGPRIHEYH 250
    KEMHNYMLSQ VPEGKEIMTV GEVGIGNEDD FRVYTSAKEG ELNMMFNFKH 300
    TSVGENPKCK YELIPFTLKD FKLALAESFL FIENTDCWST IYLENHDQPR 350
    SVSRFGSDSP KWREISSKML ATLIISLTGT VFIYQGQELG MPNFKNRKIE 400
    QIKCVEGTGT YAAIKRDYGE DSEKMKKFFE ALALISRDHG RTPFPWSADE 450
    PSAGFSKDAK PWIDMNESFR DGINAEAELK DKNSVFFFWK KALQVRKEHK 500
    DILVYGHNFQ FIDLDNDKLF MFTKDTDNKK MFAVFNFSSD NTDFSVPDNE 550
    ASYTMFFGNY ANSNGDSRTL QPWEGRLYLL K 581
    Length:581
    Mass (Da):67,557
    Last modified:February 1, 1995 - v1
    Checksum:i7B65BB7BA583ADF9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34098 Genomic DNA. Translation: CAA83995.1.
    Z49491 Genomic DNA. Translation: CAA89513.1.
    BK006943 Genomic DNA. Translation: DAA08596.1.
    PIRiS50769.
    RefSeqiNP_012319.1. NM_001181649.1.

    Genome annotation databases

    EnsemblFungiiYJL216C; YJL216C; YJL216C.
    GeneIDi853214.
    KEGGisce:YJL216C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34098 Genomic DNA. Translation: CAA83995.1 .
    Z49491 Genomic DNA. Translation: CAA89513.1 .
    BK006943 Genomic DNA. Translation: DAA08596.1 .
    PIRi S50769.
    RefSeqi NP_012319.1. NM_001181649.1.

    3D structure databases

    ProteinModelPortali P40884.
    SMRi P40884. Positions 7-579.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33544. 6 interactions.
    STRINGi 4932.YJL216C.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL216C ; YJL216C ; YJL216C .
    GeneIDi 853214.
    KEGGi sce:YJL216C.

    Organism-specific databases

    CYGDi YJL216c.
    SGDi S000003752. IMA5.

    Phylogenomic databases

    eggNOGi COG0366.
    GeneTreei ENSGT00530000063127.
    KOi K01187.
    OMAi HETESEW.
    OrthoDBi EOG7M3J7X.

    Enzyme and pathway databases

    BioCyci YEAST:YJL216C-MONOMER.

    Miscellaneous databases

    NextBioi 973404.

    Gene expression databases

    Genevestigatori P40884.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
      Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast."
      Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C.
      J. Biol. Chem. 278:11427-11432(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    5. "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family."
      Teste M.A., Francois J.M., Parrou J.L.
      J. Biol. Chem. 285:26815-26824(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiIMA5_YEAST
    AccessioniPrimary (citable) accession number: P40884
    Secondary accession number(s): D6VVY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3