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Protein

Oligo-1,6-glucosidase IMA5

Gene

IMA5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Alpha-glucosidase with specificity for isomaltose, maltose, and palatinose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei210NucleophileBy similarity1
Active sitei272Proton donorBy similarity1
Sitei347Transition state stabilizerBy similarity1

GO - Molecular functioni

  • oligo-1,6-glucosidase activity Source: SGD

GO - Biological processi

  • disaccharide catabolic process Source: SGD
  • maltose metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processMaltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YJL216C-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.
R-SCE-71240. Tryptophan catabolism.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA5 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 5
Gene namesi
Name:IMA5
Ordered Locus Names:YJL216C
ORF Names:HRF581, J0228
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL216C.
SGDiS000003752. IMA5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543311 – 581Oligo-1,6-glucosidase IMA5Add BLAST581

Proteomic databases

PRIDEiP40884.

Expressioni

Inductioni

Transcriptionally regulated by PDR8. Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi33544. 97 interactors.
STRINGi4932.YJL216C.

Structurei

3D structure databases

ProteinModelPortaliP40884.
SMRiP40884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000140965.
InParanoidiP40884.
KOiK01182.
OMAiMNNHDVP.
OrthoDBiEOG092C12JX.

Family and domain databases

InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P40884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIIHNPKWW KEATVYQIYP ASFKDSNNDG WGDLAGITSK LDYVKELGVD
60 70 80 90 100
AIWVCPFYDS PQEDMGYDIA NYEKVWPRYG TNEDCFQMIE EAHKRGIKVI
110 120 130 140 150
VDLVINHCSE EHEWFKESRS SKANPKRDWF FWRPPKGYDE KGNPIPPNNW
160 170 180 190 200
RSFFGGSAWR YDEKTGEFFL HVFALGQPDF NWENEECRKA IYDSSVGYWL
210 220 230 240 250
RHNVDGFRID VGSMYSKVEG LPDAPITDPT VPYQKGTEFF INGPRIHEYH
260 270 280 290 300
KEMHNYMLSQ VPEGKEIMTV GEVGIGNEDD FRVYTSAKEG ELNMMFNFKH
310 320 330 340 350
TSVGENPKCK YELIPFTLKD FKLALAESFL FIENTDCWST IYLENHDQPR
360 370 380 390 400
SVSRFGSDSP KWREISSKML ATLIISLTGT VFIYQGQELG MPNFKNRKIE
410 420 430 440 450
QIKCVEGTGT YAAIKRDYGE DSEKMKKFFE ALALISRDHG RTPFPWSADE
460 470 480 490 500
PSAGFSKDAK PWIDMNESFR DGINAEAELK DKNSVFFFWK KALQVRKEHK
510 520 530 540 550
DILVYGHNFQ FIDLDNDKLF MFTKDTDNKK MFAVFNFSSD NTDFSVPDNE
560 570 580
ASYTMFFGNY ANSNGDSRTL QPWEGRLYLL K
Length:581
Mass (Da):67,557
Last modified:February 1, 1995 - v1
Checksum:i7B65BB7BA583ADF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83995.1.
Z49491 Genomic DNA. Translation: CAA89513.1.
BK006943 Genomic DNA. Translation: DAA08596.1.
PIRiS50769.
RefSeqiNP_012319.1. NM_001181649.1.

Genome annotation databases

EnsemblFungiiYJL216C; YJL216C; YJL216C.
GeneIDi853214.
KEGGisce:YJL216C.

Similar proteinsi

Entry informationi

Entry nameiIMA5_YEAST
AccessioniPrimary (citable) accession number: P40884
Secondary accession number(s): D6VVY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names