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P40884

- IMA5_YEAST

UniProt

P40884 - IMA5_YEAST

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Protein

Oligo-1,6-glucosidase IMA5

Gene

IMA5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Alpha-glucosidase with specificity for isomaltose, maltose, and palatinose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei210 – 2101NucleophileBy similarity
Active sitei272 – 2721Proton donorBy similarity
Sitei347 – 3471Transition state stabilizerBy similarity

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: SGD

GO - Biological processi

  1. disaccharide catabolic process Source: SGD
  2. maltose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YJL216C-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA5 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 5
Gene namesi
Name:IMA5
Ordered Locus Names:YJL216C
ORF Names:HRF581, J0228
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL216c.
SGDiS000003752. IMA5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Oligo-1,6-glucosidase IMA5PRO_0000054331Add
BLAST

Expressioni

Inductioni

Transcriptionally regulated by PDR8. Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside.2 Publications

Gene expression databases

GenevestigatoriP40884.

Interactioni

Protein-protein interaction databases

BioGridi33544. 6 interactions.
STRINGi4932.YJL216C.

Structurei

3D structure databases

ProteinModelPortaliP40884.
SMRiP40884. Positions 7-579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiCOG0366.
GeneTreeiENSGT00530000063127.
InParanoidiP40884.
KOiK01187.
OMAiHETESEW.
OrthoDBiEOG7M3J7X.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P40884-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTIIHNPKWW KEATVYQIYP ASFKDSNNDG WGDLAGITSK LDYVKELGVD
60 70 80 90 100
AIWVCPFYDS PQEDMGYDIA NYEKVWPRYG TNEDCFQMIE EAHKRGIKVI
110 120 130 140 150
VDLVINHCSE EHEWFKESRS SKANPKRDWF FWRPPKGYDE KGNPIPPNNW
160 170 180 190 200
RSFFGGSAWR YDEKTGEFFL HVFALGQPDF NWENEECRKA IYDSSVGYWL
210 220 230 240 250
RHNVDGFRID VGSMYSKVEG LPDAPITDPT VPYQKGTEFF INGPRIHEYH
260 270 280 290 300
KEMHNYMLSQ VPEGKEIMTV GEVGIGNEDD FRVYTSAKEG ELNMMFNFKH
310 320 330 340 350
TSVGENPKCK YELIPFTLKD FKLALAESFL FIENTDCWST IYLENHDQPR
360 370 380 390 400
SVSRFGSDSP KWREISSKML ATLIISLTGT VFIYQGQELG MPNFKNRKIE
410 420 430 440 450
QIKCVEGTGT YAAIKRDYGE DSEKMKKFFE ALALISRDHG RTPFPWSADE
460 470 480 490 500
PSAGFSKDAK PWIDMNESFR DGINAEAELK DKNSVFFFWK KALQVRKEHK
510 520 530 540 550
DILVYGHNFQ FIDLDNDKLF MFTKDTDNKK MFAVFNFSSD NTDFSVPDNE
560 570 580
ASYTMFFGNY ANSNGDSRTL QPWEGRLYLL K
Length:581
Mass (Da):67,557
Last modified:February 1, 1995 - v1
Checksum:i7B65BB7BA583ADF9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z34098 Genomic DNA. Translation: CAA83995.1.
Z49491 Genomic DNA. Translation: CAA89513.1.
BK006943 Genomic DNA. Translation: DAA08596.1.
PIRiS50769.
RefSeqiNP_012319.1. NM_001181649.1.

Genome annotation databases

EnsemblFungiiYJL216C; YJL216C; YJL216C.
GeneIDi853214.
KEGGisce:YJL216C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z34098 Genomic DNA. Translation: CAA83995.1 .
Z49491 Genomic DNA. Translation: CAA89513.1 .
BK006943 Genomic DNA. Translation: DAA08596.1 .
PIRi S50769.
RefSeqi NP_012319.1. NM_001181649.1.

3D structure databases

ProteinModelPortali P40884.
SMRi P40884. Positions 7-579.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33544. 6 interactions.
STRINGi 4932.YJL216C.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL216C ; YJL216C ; YJL216C .
GeneIDi 853214.
KEGGi sce:YJL216C.

Organism-specific databases

CYGDi YJL216c.
SGDi S000003752. IMA5.

Phylogenomic databases

eggNOGi COG0366.
GeneTreei ENSGT00530000063127.
InParanoidi P40884.
KOi K01187.
OMAi HETESEW.
OrthoDBi EOG7M3J7X.

Enzyme and pathway databases

BioCyci YEAST:YJL216C-MONOMER.

Miscellaneous databases

NextBioi 973404.

Gene expression databases

Genevestigatori P40884.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
    Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast."
    Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C.
    J. Biol. Chem. 278:11427-11432(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family."
    Teste M.A., Francois J.M., Parrou J.L.
    J. Biol. Chem. 285:26815-26824(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiIMA5_YEAST
AccessioniPrimary (citable) accession number: P40884
Secondary accession number(s): D6VVY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3