P40884 (IMA5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligo-1,6-glucosidase IMA5
EC=3.2.1.10

Alternative name(s):
Alpha-glucosidase
Isomaltase 5

Gene names

Name:	IMA5
Ordered Locus Names:	YJL216C
ORF Names:	HRF581, J0228

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Alpha-glucosidase with specificity for isomaltose, maltose, and palatinose. Ref.5
Catalytic activity	Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Induction	Transcriptionally regulated by PDR8. Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside. Ref.4 Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Maltose metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	disaccharide catabolic process Inferred from genetic interaction PubMed 20471265Ref.5. Source: SGD maltose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro oligo-1,6-glucosidase activity Inferred from direct assay PubMed 20471265. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Oligo-1,6-glucosidase IMA5		PRO_0000054331
Sites
	Active site	210	1	Nucleophile By similarity
	Active site	272	1	Proton donor By similarity
	Site	347	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40884 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7B65BB7BA583ADF9	FASTA	581	67,557
	10 20 30 40 50 60 MTIIHNPKWW KEATVYQIYP ASFKDSNNDG WGDLAGITSK LDYVKELGVD AIWVCPFYDS 70 80 90 100 110 120 PQEDMGYDIA NYEKVWPRYG TNEDCFQMIE EAHKRGIKVI VDLVINHCSE EHEWFKESRS 130 140 150 160 170 180 SKANPKRDWF FWRPPKGYDE KGNPIPPNNW RSFFGGSAWR YDEKTGEFFL HVFALGQPDF 190 200 210 220 230 240 NWENEECRKA IYDSSVGYWL RHNVDGFRID VGSMYSKVEG LPDAPITDPT VPYQKGTEFF 250 260 270 280 290 300 INGPRIHEYH KEMHNYMLSQ VPEGKEIMTV GEVGIGNEDD FRVYTSAKEG ELNMMFNFKH 310 320 330 340 350 360 TSVGENPKCK YELIPFTLKD FKLALAESFL FIENTDCWST IYLENHDQPR SVSRFGSDSP 370 380 390 400 410 420 KWREISSKML ATLIISLTGT VFIYQGQELG MPNFKNRKIE QIKCVEGTGT YAAIKRDYGE 430 440 450 460 470 480 DSEKMKKFFE ALALISRDHG RTPFPWSADE PSAGFSKDAK PWIDMNESFR DGINAEAELK 490 500 510 520 530 540 DKNSVFFFWK KALQVRKEHK DILVYGHNFQ FIDLDNDKLF MFTKDTDNKK MFAVFNFSSD 550 560 570 580 NTDFSVPDNE ASYTMFFGNY ANSNGDSRTL QPWEGRLYLL K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X." Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:1657-1662(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast." Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C. J. Biol. Chem. 278:11427-11432(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[5]	"Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family." Teste M.A., Francois J.M., Parrou J.L. J. Biol. Chem. 285:26815-26824(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z34098 Genomic DNA. Translation: CAA83995.1. Z49491 Genomic DNA. Translation: CAA89513.1. BK006943 Genomic DNA. Translation: DAA08596.1.
PIR	S50769.
RefSeq	NP_012319.1. NM_001181649.1.
3D structure databases
ProteinModelPortal	P40884.
SMR	P40884. Positions 7-579.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YJL216C.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
mycoCLAP	OGL13E_YEAST.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL216C; YJL216C; YJL216C.
GeneID	853214.
KEGG	sce:YJL216C.
Organism-specific databases
CYGD	YJL216c.
SGD	S000003752. YJL216C.
Phylogenomic databases
eggNOG	COG0366.
GeneTree	ENSGT00530000063127.
KO	K01187.
OMA	VRNIEIM.
OrthoDB	EOG4K0TWQ.
Gene expression databases
ArrayExpress	P40884.
Genevestigator	P40884.
GermOnline	YJL216C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
NextBio	973404.

Entry information

Entry name

IMA5_YEAST

Accession

Primary (citable) accession number: P40884
Secondary accession number(s): D6VVY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program