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Protein

Oligo-1,6-glucosidase IMA5

Gene

IMA5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Alpha-glucosidase with specificity for isomaltose, maltose, and palatinose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei210 – 2101NucleophileBy similarity
Active sitei272 – 2721Proton donorBy similarity
Sitei347 – 3471Transition state stabilizerBy similarity

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: SGD

GO - Biological processi

  1. disaccharide catabolic process Source: SGD
  2. maltose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YJL216C-MONOMER.
ReactomeiREACT_341203. Amino acid transport across the plasma membrane.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA5 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 5
Gene namesi
Name:IMA5
Ordered Locus Names:YJL216C
ORF Names:HRF581, J0228
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL216c.
SGDiS000003752. IMA5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Oligo-1,6-glucosidase IMA5PRO_0000054331Add
BLAST

Expressioni

Inductioni

Transcriptionally regulated by PDR8. Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside.2 Publications

Gene expression databases

ExpressionAtlasiP40884. differential.
GenevestigatoriP40884.

Interactioni

Protein-protein interaction databases

BioGridi33544. 6 interactions.
STRINGi4932.YJL216C.

Structurei

3D structure databases

ProteinModelPortaliP40884.
SMRiP40884. Positions 7-579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiCOG0366.
GeneTreeiENSGT00530000063127.
InParanoidiP40884.
KOiK01187.
OMAiWERARWA.
OrthoDBiEOG7M3J7X.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P40884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIIHNPKWW KEATVYQIYP ASFKDSNNDG WGDLAGITSK LDYVKELGVD
60 70 80 90 100
AIWVCPFYDS PQEDMGYDIA NYEKVWPRYG TNEDCFQMIE EAHKRGIKVI
110 120 130 140 150
VDLVINHCSE EHEWFKESRS SKANPKRDWF FWRPPKGYDE KGNPIPPNNW
160 170 180 190 200
RSFFGGSAWR YDEKTGEFFL HVFALGQPDF NWENEECRKA IYDSSVGYWL
210 220 230 240 250
RHNVDGFRID VGSMYSKVEG LPDAPITDPT VPYQKGTEFF INGPRIHEYH
260 270 280 290 300
KEMHNYMLSQ VPEGKEIMTV GEVGIGNEDD FRVYTSAKEG ELNMMFNFKH
310 320 330 340 350
TSVGENPKCK YELIPFTLKD FKLALAESFL FIENTDCWST IYLENHDQPR
360 370 380 390 400
SVSRFGSDSP KWREISSKML ATLIISLTGT VFIYQGQELG MPNFKNRKIE
410 420 430 440 450
QIKCVEGTGT YAAIKRDYGE DSEKMKKFFE ALALISRDHG RTPFPWSADE
460 470 480 490 500
PSAGFSKDAK PWIDMNESFR DGINAEAELK DKNSVFFFWK KALQVRKEHK
510 520 530 540 550
DILVYGHNFQ FIDLDNDKLF MFTKDTDNKK MFAVFNFSSD NTDFSVPDNE
560 570 580
ASYTMFFGNY ANSNGDSRTL QPWEGRLYLL K
Length:581
Mass (Da):67,557
Last modified:January 31, 1995 - v1
Checksum:i7B65BB7BA583ADF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83995.1.
Z49491 Genomic DNA. Translation: CAA89513.1.
BK006943 Genomic DNA. Translation: DAA08596.1.
PIRiS50769.
RefSeqiNP_012319.1. NM_001181649.1.

Genome annotation databases

EnsemblFungiiYJL216C; YJL216C; YJL216C.
GeneIDi853214.
KEGGisce:YJL216C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34098 Genomic DNA. Translation: CAA83995.1.
Z49491 Genomic DNA. Translation: CAA89513.1.
BK006943 Genomic DNA. Translation: DAA08596.1.
PIRiS50769.
RefSeqiNP_012319.1. NM_001181649.1.

3D structure databases

ProteinModelPortaliP40884.
SMRiP40884. Positions 7-579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33544. 6 interactions.
STRINGi4932.YJL216C.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL216C; YJL216C; YJL216C.
GeneIDi853214.
KEGGisce:YJL216C.

Organism-specific databases

CYGDiYJL216c.
SGDiS000003752. IMA5.

Phylogenomic databases

eggNOGiCOG0366.
GeneTreeiENSGT00530000063127.
InParanoidiP40884.
KOiK01187.
OMAiWERARWA.
OrthoDBiEOG7M3J7X.

Enzyme and pathway databases

BioCyciYEAST:YJL216C-MONOMER.
ReactomeiREACT_341203. Amino acid transport across the plasma membrane.

Miscellaneous databases

NextBioi973404.

Gene expression databases

ExpressionAtlasiP40884. differential.
GenevestigatoriP40884.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X."
    Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:1657-1662(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast."
    Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C.
    J. Biol. Chem. 278:11427-11432(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family."
    Teste M.A., Francois J.M., Parrou J.L.
    J. Biol. Chem. 285:26815-26824(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiIMA5_YEAST
AccessioniPrimary (citable) accession number: P40884
Secondary accession number(s): D6VVY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1995
Last sequence update: January 31, 1995
Last modified: March 31, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.