Reviewed, UniProtKB/Swiss-Prot P40884 (MALX1_YEAST)

Names and origin

Protein names

Recommended name:
    Probable alpha-glucosidase YJL216C
    EC=3.2.1.20
Alternative name(s):
    Maltase

Gene names

Ordered Locus Names:	YJL216C
ORF Names:	J0228, HRF581

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable alpha-glucosidase involved in maltose metabolism.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Induction	Transcriptionally regulated by PDR8. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Maltose metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	maltose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Probable alpha-glucosidase YJL216C		PRO_0000054331
Sites
	Active site	210	1	Nucleophile By similarity
	Active site	272	1	Proton donor By similarity
	Active site	347	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40884-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7B65BB7BA583ADF9	FASTA	581	67,557
	10 20 30 40 50 60 MTIIHNPKWW KEATVYQIYP ASFKDSNNDG WGDLAGITSK LDYVKELGVD AIWVCPFYDS 70 80 90 100 110 120 PQEDMGYDIA NYEKVWPRYG TNEDCFQMIE EAHKRGIKVI VDLVINHCSE EHEWFKESRS 130 140 150 160 170 180 SKANPKRDWF FWRPPKGYDE KGNPIPPNNW RSFFGGSAWR YDEKTGEFFL HVFALGQPDF 190 200 210 220 230 240 NWENEECRKA IYDSSVGYWL RHNVDGFRID VGSMYSKVEG LPDAPITDPT VPYQKGTEFF 250 260 270 280 290 300 INGPRIHEYH KEMHNYMLSQ VPEGKEIMTV GEVGIGNEDD FRVYTSAKEG ELNMMFNFKH 310 320 330 340 350 360 TSVGENPKCK YELIPFTLKD FKLALAESFL FIENTDCWST IYLENHDQPR SVSRFGSDSP 370 380 390 400 410 420 KWREISSKML ATLIISLTGT VFIYQGQELG MPNFKNRKIE QIKCVEGTGT YAAIKRDYGE 430 440 450 460 470 480 DSEKMKKFFE ALALISRDHG RTPFPWSADE PSAGFSKDAK PWIDMNESFR DGINAEAELK 490 500 510 520 530 540 DKNSVFFFWK KALQVRKEHK DILVYGHNFQ FIDLDNDKLF MFTKDTDNKK MFAVFNFSSD 550 560 570 580 NTDFSVPDNE ASYTMFFGNY ANSNGDSRTL QPWEGRLYLL K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X." Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:1657-1662(1994) [PubMed: 7725802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast." Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C. J. Biol. Chem. 278:11427-11432(2003) [PubMed: 12529331] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z34098 Genomic DNA. Translation: CAA83995.1. Z49491 Genomic DNA. Translation: CAA89513.1.
PIR	S50769.
RefSeq	NP_012319.1.
3D structure databases
HSSP	HSSP built from PDB template 1UOK based on UniProtKB P21332.
ModBase	Search...
Protein-protein interaction databases
STRING	P40884.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
Ensembl	YJL216C; YJL216C; YJL216C; Saccharomyces cerevisiae. [Genome view]
GeneID	853214.
GenomeReviews	Gene locus YJL216C in contig Y13136_GR.
KEGG	sce:YJL216C.
NMPDR	fig|4932.3.peg.3282.
Organism-specific databases
CYGD	YJL216c.
SGD	S000003752. YJL216C.
Phylogenomic databases
HOGENOM	P40884.
Enzyme and pathway databases
BRENDA	3.2.1.20. 250.
Gene expression databases
ArrayExpress	P40884.
Genevestigator	P40884.
GermOnline	YJL216C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973404.

Entry information

Entry name

MALX1_YEAST

Accession

Primary (citable) accession number: P40884

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	October 13, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)