Reviewed, UniProtKB/Swiss-Prot P40875 (SOXB_CORS1)

Names and origin

Protein names

Recommended name:
    Sarcosine oxidase subunit beta
      Short name=Sarcosine oxidase subunit B
    EC=1.5.3.1

Gene names

Name:

soxB

Organism

Corynebacterium sp. (strain P-1)

Taxonomic identifier

69006 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
Catalytic activity	Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2).
Cofactor	Binds 1 FAD per subunit. Binds 1 FMN covalently.
Subunit structure	Heterotetramer (alpha 100 kDa, beta 42 kDa, gamma 20 kDa, and delta 6 kDa).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the soxB family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sarcosine oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Sarcosine oxidase subunit beta		PRO_0000072044
Amino acid modifications
	Modified residue	173	1	Tele-8alpha-FMN histidine
Natural variations
	Natural variant	167	1	R → Y in strain: U-96.
	Natural variant	172 – 173	2	KH → CK in strain: U-96.
	Natural variant	178	1	W → T in strain: U-96.
	Natural variant	182	1	R → N in strain: U-96.
	Natural variant	359	1	K → FC in strain: U-96.
	Natural variant	366	1	F → Y in strain: U-96.
	Natural variant	370	1	H → E in strain: U-96.
	Natural variant	374	1	N → A in strain: U-96.
	Natural variant	377 – 379	3	AHA → PKK in strain: U-96.
	Natural variant	385	1	S → A in strain: U-96.

Sequences

	Sequence		Length	Mass (Da)
	P40875-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 401141810BA60CDE	FASTA	405	43,987
	10 20 30 40 50 60 MADLLPEHPE FLWANPEPKK SYDVVIVGGG GHGLATAYYL AKNHGITNVA VLEKGWLAGG 70 80 90 100 110 120 NMARNTTIIR SNYLWDESAG IYEKSLKLWE QLPEELDYDF LFSQRGVLNL AHTLGDVRES 130 140 150 160 170 180 VRRVEANKFN GVDAEWLTPE QVKEVCPIIN IGDDIRYPVM GATYQPRAGI AKHDHVAWAF 190 200 210 220 230 240 ARKANEMGVD IIQNCEVTGF LKDGEKVTGV KTTRGTIHAG KVALAGAGHS SVLAELAGFE 250 260 270 280 290 300 LPIQSHPLQA LVSELFEPVH PTVVMSNHIH VYVSQAHKGE LVMGAGIDSY NGYGQRGAFH 310 320 330 340 350 360 VIEEQMAAAV ELFPIFARAH VLRTWGGIVD TTMDASPIIS KTPIQNLYVN CGWGTGGFKG 370 380 390 400 TPGAGFTLAH TIANDEAHAL NAPFSLERFE TGHLIDEHGA AAVAH

References

[1]	"Sequence analysis of sarcosine oxidase and nearby genes reveals homologies with key enzymes of folate one-carbon metabolism." Chlumsky L.J., Zhang L., Jorns M.S. J. Biol. Chem. 270:18252-18259(1995) [PubMed: 7543100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cysteine residues in the active site of Corynebacterium sarcosine oxidase." Suzuki H., Kawamura-Konishi Y. J. Biochem. 109:909-917(1991) [PubMed: 1939012] [Abstract] Cited for: PROTEIN SEQUENCE OF 161-187 AND 334-404. Strain: U-96.
[3]	"Presence of AMP binding sequence in subunit B of Corynebacterium sarcosine oxidase." Suzuki H., Kawamura-Konishi Y. Biochem. Int. 17:577-583(1988) [PubMed: 3202887] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: U-96.
[4]	"Preparation and properties of recombinant corynebacterial sarcosine oxidase: evidence for posttranslational modification during turnover with sarcosine." Chlumsky L.J., Zhang L., Ramsey A.J., Jorns M.S. Biochemistry 32:11132-11142(1993) [PubMed: 7692961] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Sarcosine oxidase contains a novel covalently bound FMN." Willie A., Edmondson D.E., Jorns M.S. Biochemistry 35:5292-5299(1996) [PubMed: 8611516] [Abstract] Cited for: FMN-BINDING.
[6]	"Identification of the covalent flavin attachment site in sarcosine oxidase." Chlumsky L.J., Sturgess A.W., Nieves E., Jorns M.S. Biochemistry 37:2089-2095(1998) [PubMed: 9485355] [Abstract] Cited for: PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, MASS SPECTROMETRY.
[7]	"Cofactors in sarcosine oxidase from Corynebacterium sp. U-96." Mukouyama E.B., Ohsawa H., Suzuki H. J. Protein Chem. 21:59-64(2002) [PubMed: 11902668] [Abstract] Cited for: PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23955 Genomic DNA. Translation: AAC43459.1.
PIR	PX0049.
3D structure databases
SMR	P40875. Positions 3-405.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-8522.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR006278. SoxB. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR01373. soxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SOXB_CORS1

Accession

Primary (citable) accession number: P40875
Secondary accession number(s): Q46335

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)