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Protein

Sarcosine oxidase subunit beta

Gene

soxB

Organism
Corynebacterium sp. (strain P-1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.

Catalytic activityi

Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.

Cofactori

Protein has several cofactor binding sites:
  • FADNote: Binds 1 FAD per subunit.
  • FMNNote: Binds 1 FMN covalently.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, FMN

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8522.

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcosine oxidase subunit beta (EC:1.5.3.1)
Short name:
Sarcosine oxidase subunit B
Gene namesi
Name:soxB
OrganismiCorynebacterium sp. (strain P-1)
Taxonomic identifieri69006 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi175H → A: No effect on FMN binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000720442 – 405Sarcosine oxidase subunit betaAdd BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei173Tele-8alpha-FMN histidine1

Interactioni

Subunit structurei

Heterotetramer (SoxA, SoxB, SoxG and SoxD).

Structurei

3D structure databases

ProteinModelPortaliP40875.
SMRiP40875.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 53ADP bindingSequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the SoxB family.Curated

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiView protein in InterPro
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR006278. SoxB.
PfamiView protein in Pfam
PF01266. DAO. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01373. soxB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40875-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLLPEHPE FLWANPEPKK SYDVVIVGGG GHGLATAYYL AKNHGITNVA
60 70 80 90 100
VLEKGWLAGG NMARNTTIIR SNYLWDESAG IYEKSLKLWE QLPEELDYDF
110 120 130 140 150
LFSQRGVLNL AHTLGDVRES VRRVEANKFN GVDAEWLTPE QVKEVCPIIN
160 170 180 190 200
IGDDIRYPVM GATYQPRAGI AKHDHVAWAF ARKANEMGVD IIQNCEVTGF
210 220 230 240 250
LKDGEKVTGV KTTRGTIHAG KVALAGAGHS SVLAELAGFE LPIQSHPLQA
260 270 280 290 300
LVSELFEPVH PTVVMSNHIH VYVSQAHKGE LVMGAGIDSY NGYGQRGAFH
310 320 330 340 350
VIEEQMAAAV ELFPIFARAH VLRTWGGIVD TTMDASPIIS KTPIQNLYVN
360 370 380 390 400
CGWGTGGFKG TPGAGFTLAH TIANDEAHAL NAPFSLERFE TGHLIDEHGA

AAVAH
Length:405
Mass (Da):43,987
Last modified:November 1, 1997 - v2
Checksum:i401141810BA60CDE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti167R → Y in strain: U-96. 1
Natural varianti172 – 173KH → CK in strain: U-96. 2
Natural varianti178W → T in strain: U-96. 1
Natural varianti182R → N in strain: U-96. 1
Natural varianti359K → FC in strain: U-96. 1
Natural varianti366F → Y in strain: U-96. 1
Natural varianti370H → E in strain: U-96. 1
Natural varianti374N → A in strain: U-96. 1
Natural varianti377 – 379AHA → PKK in strain: U-96. 3
Natural varianti385S → A in strain: U-96. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23955 Genomic DNA. Translation: AAC43459.1.
PIRiPX0049.

Similar proteinsi

Entry informationi

Entry nameiSOXB_CORS1
AccessioniPrimary (citable) accession number: P40875
Secondary accession number(s): Q46335
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: April 12, 2017
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families