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Protein

N-methyl-L-tryptophan oxidase

Gene

solA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative demethylation of N-methyl-L-tryptophan. Can also use other N-methyl amino acids, including sarcosine, which, however, is a poor substrate.

Catalytic activityi

N-methyl-L-tryptophan + H2O + O2 = L-tryptophan + formaldehyde + H2O2.

Cofactori

FADNote: Binds 1 FAD per subunit.

Enzyme regulationi

Aromatic carboxylates are competitive inhibitors.

pH dependencei

Optimum pH is 8.0.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi4 – 34FADSequence analysisAdd BLAST31

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: EcoCyc
  • N-methyl-L-amino-acid oxidase activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:SARCOX-MONOMER
MetaCyc:SARCOX-MONOMER
SABIO-RKiP40874

Names & Taxonomyi

Protein namesi
Recommended name:
N-methyl-L-tryptophan oxidase (EC:1.5.3.-)
Short name:
MTOX
Gene namesi
Name:solA
Ordered Locus Names:b1059, JW1046
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12669 solA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002137651 – 372N-methyl-L-tryptophan oxidaseAdd BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei308S-8alpha-FAD cysteine1

Proteomic databases

EPDiP40874
PaxDbiP40874
PRIDEiP40874

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi426175481 interactors.
IntActiP40874 9 interactors.
STRINGi316385.ECDH10B_1130

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 23Combined sources12
Beta strandi28 – 31Combined sources4
Beta strandi36 – 42Combined sources7
Beta strandi45 – 50Combined sources6
Helixi58 – 60Combined sources3
Helixi61 – 76Combined sources16
Beta strandi79 – 81Combined sources3
Beta strandi83 – 85Combined sources3
Beta strandi89 – 94Combined sources6
Helixi98 – 109Combined sources12
Beta strandi114 – 118Combined sources5
Helixi119 – 125Combined sources7
Beta strandi135 – 142Combined sources8
Beta strandi144 – 147Combined sources4
Helixi148 – 161Combined sources14
Beta strandi165 – 167Combined sources3
Beta strandi172 – 177Combined sources6
Beta strandi179 – 188Combined sources10
Beta strandi190 – 198Combined sources9
Helixi201 – 206Combined sources6
Beta strandi214 – 217Combined sources4
Beta strandi220 – 223Combined sources4
Helixi227 – 229Combined sources3
Turni231 – 234Combined sources4
Beta strandi237 – 241Combined sources5
Beta strandi247 – 251Combined sources5
Beta strandi254 – 256Combined sources3
Beta strandi258 – 264Combined sources7
Helixi272 – 274Combined sources3
Helixi284 – 286Combined sources3
Helixi289 – 295Combined sources7
Beta strandi302 – 305Combined sources4
Beta strandi309 – 312Combined sources4
Beta strandi320 – 323Combined sources4
Beta strandi326 – 332Combined sources7
Helixi340 – 342Combined sources3
Helixi343 – 354Combined sources12
Helixi364 – 366Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UZZX-ray3.20A/B/C/D1-372[»]
ProteinModelPortaliP40874
SMRiP40874
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40874

Family & Domainsi

Sequence similaritiesi

Belongs to the MSOX/MTOX family. MTOX subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105F76 Bacteria
COG0665 LUCA
HOGENOMiHOG000236098
InParanoidiP40874
KOiK02846
OMAiRIIRHAY
PhylomeDBiP40874

Family and domain databases

Gene3Di3.50.50.603 hits
HAMAPiMF_00515 MTOX, 1 hit
InterProiView protein in InterPro
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR023493 Me_Trp_Oxase_MTOX
PfamiView protein in Pfam
PF01266 DAO, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

P40874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYDLIIIGS GSVGAAAGYY ATRAGLNVLM TDAHMPPHQH GSHHGDTRLI
60 70 80 90 100
RHAYGEGEKY VPLVLRAQTL WDELSRHNEE DPIFVRSGVI NLGPADSTFL
110 120 130 140 150
ANVAHSAEQW QLNVEKLDAQ GIMARWPEIR VPDNYIGLFE TDSGFLRSEL
160 170 180 190 200
AIKTWIQLAK EAGCAQLFNC PVTAIRHDDD GVTIETADGE YQAKKAIVCA
210 220 230 240 250
GTWVKDLLPE LPVQPVRKVF AWYQADGRYS VKNKFPAFTG ELPNGDQYYG
260 270 280 290 300
FPAENDALKI GKHNGGQVIH SADERVPFAE VASDGSEAFP FLRNVLPGIG
310 320 330 340 350
CCLYGAACTY DNSPDEDFII DTLPGHDNTL LITGLSGHGF KFASVLGEIA
360 370
ADFAQDKKSD FDLTPFRLSR FQ
Length:372
Mass (Da):40,902
Last modified:November 1, 1997 - v1
Checksum:iDF5F4411D71610FD
GO

Mass spectrometryi

Molecular mass is 41684 Da from positions 1 - 372. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31709 Genomic DNA Translation: BAA06516.1
U00096 Genomic DNA Translation: AAC74143.1
AP009048 Genomic DNA Translation: BAA35856.1
PIRiJC5371
RefSeqiNP_415577.1, NC_000913.3
WP_000872833.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74143; AAC74143; b1059
BAA35856; BAA35856; BAA35856
GeneIDi944983
KEGGiecj:JW1046
eco:b1059
PATRICifig|1411691.4.peg.1209

Similar proteinsi

Entry informationi

Entry nameiMTOX_ECOLI
AccessioniPrimary (citable) accession number: P40874
Secondary accession number(s): Q47144
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome