Reviewed, UniProtKB/Swiss-Prot P40873 (MSOX_ARTST)

Names and origin

Protein names

Recommended name:
    Monomeric sarcosine oxidase
      Short name=MSOX
    EC=1.5.3.1

Gene names

Name:

soxA

Organism

Arthrobacter sp. (strain TE1826)

Taxonomic identifier

68999 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidative demethylation of sarcosine.
Catalytic activity	Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2).
Cofactor	Binds 1 FAD per subunit.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Miscellaneous	Decreases in function by replacement in the G-X-G-X-X-G motif are suppressed by chloride or bromide ion.
Sequence similarities	Belongs to the MSOX/MTOX family. MSOX subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	sarcosine oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 389	388	Monomeric sarcosine oxidase		PRO_0000213760
Regions
	Nucleotide binding	8 – 38	31	FAD Potential
Amino acid modifications
	Modified residue	318	1	S-8alpha-FAD cysteine Probable
Experimental info
	Mutagenesis	13	1	G → A: Loss of activity
	Mutagenesis	14	1	A → D or K: Loss of activity
	Mutagenesis	14	1	A → I or V: Decrease in activity
	Mutagenesis	14	1	A → L, W or Y: Weak activity
	Mutagenesis	15	1	G → A: Loss of activity
	Mutagenesis	16	1	S → P: Weak activity. Loss of activity; when associated with G-17
	Mutagenesis	17	1	M → G: Weak activity. Loss of activity; when associated with P-16
	Mutagenesis	18	1	G → A: Weak activity
	Mutagenesis	36	1	D → A: Loss of activity
	Mutagenesis	36	1	D → E: Almost no change in activity
	Mutagenesis	36	1	D → N: 100-fold decrease in activity
	Mutagenesis	265	1	C → A or D: Decrease in activity. Stabilizes soxA against chemicals and metal ions
	Mutagenesis	265	1	C → R or S: Almost no change in activity. Stabilizes soxA against chemicals and metal ions
	Mutagenesis	318	1	C → S: Weak activity
	Sequence conflict	39	1	H → D in BAA09716 and BAA25926. Ref.2
	Sequence conflict	316	1	D → A in BAA09716 and BAA25926. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P40873-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 77437C4F12B4AC7F	FASTA	389	43,294
	10 20 30 40 50 60 MSIKKDYDVI VVGAGSMGMA AGYYLSKQGV KTLLVDSFHP PHTNGSHHGD TRIIRHAYGE 70 80 90 100 110 120 GREYVPFALR AQELWYELEK ETHHKIFTKT GVLVFGPKGE APFVAETMEA AKEHSLDVDL 130 140 150 160 170 180 LEGSEINKRW PGVTVPENYN AIFEKNSGVL FSENCIRAYR ELAEANGAKV LTYTPVEDFE 190 200 210 220 230 240 IAEDFVKIQT AYGSFTASKL IVSMGAWNSK LLSKLNIEIP LQPYRQVVGF FECDEKKYSN 250 260 270 280 290 300 THGYPAFMVE VPTGIYYGFP SFGGCGLKIG YHTYGQKIDP DTINREFGIY PEDEGNIRKF 310 320 330 340 350 360 LETYMPGATG ELKSGDVCMY TKTPDEHFVI DLHPQFSNVA IAAGFSGHGF KFSSVVGETL 370 380 SQLAVTGKTE HDISIFSINR PALKQKETI

References

[1]	"Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826." Nishiya Y., Imanaka T. J. Ferment. Bioeng. 75:239-244(1993) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-6.
[2]	"Gene cluster for creatinine degradation in Arthrobacter sp. TE1826." Nishiya Y., Toda A., Imanaka T. Mol. Gen. Genet. 257:581-586(1998) [PubMed: 9563845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Active site analysis and stabilization of sarcosine oxidase by the substitution of cysteine residues." Nishiya Y., Zuihara S., Imanaka T. Appl. Environ. Microbiol. 61:367-370(1995) [PubMed: 7887617] [Abstract] Cited for: MUTAGENESIS OF CYS-265 AND CYS-318.
[4]	"Analysis of interaction between the Arthrobacter sarcosine oxidase and the coenzyme flavin adenine dinucleotide by site-directed mutagenesis." Nishiya Y., Imanaka T. Appl. Environ. Microbiol. 62:2405-2410(1996) [PubMed: 8779579] [Abstract] Cited for: MUTAGENESIS OF GLY-13; ALA-14; GLY-15; SER-16; MET-17; GLY-18 AND ASP-36.
[5]	"A mutant sarcosine oxidase in which activity depends on flavin adenine dinucleotide." Nishiya Y. Protein Expr. Purif. 20:95-97(2000) [PubMed: 11035956] [Abstract] Cited for: MUTAGENESIS OF CYS-318.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D63413 Genomic DNA. Translation: BAA09716.1. AB007122 Genomic DNA. Translation: BAA25926.1.
PIR	T44248.
3D structure databases
HSSP	HSSP built from PDB template 1L9F based on UniProtKB P40859.
SMR	P40873. Positions 7-385.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11002.
Family and domain databases
HAMAP	MF_00516. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR006281. SoxA_mon. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR01377. soxA_mon. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSOX_ARTST

Accession

Primary (citable) accession number: P40873
Secondary accession number(s): O66180

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)