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P40871

- DHBE_BACSU

UniProt

P40871 - DHBE_BACSU

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Protein

2,3-dihydroxybenzoate-AMP ligase

Gene

dhbE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Activation of the carboxylate group of 2,3-dihydroxy-benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei240 – 2401Substrate
Binding sitei329 – 3291ATP; via carbonyl oxygen
Binding sitei413 – 4131ATP
Binding sitei428 – 4281ATP
Binding sitei519 – 5191ATP
Binding sitei519 – 5191Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi191 – 1922ATP
Nucleotide bindingi307 – 3093ATP

GO - Molecular functioni

  1. (2,3-dihydroxybenzoyl)adenylate synthase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ligase activity Source: UniProtKB-KW

GO - Biological processi

  1. siderophore biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU31980-MONOMER.
MetaCyc:MONOMER-13920.
SABIO-RKP40871.
UniPathwayiUPA00013.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-dihydroxybenzoate-AMP ligase (EC:6.3.2.-)
Alternative name(s):
Dihydroxybenzoic acid-activating enzyme
Gene namesi
Name:dhbE
Synonyms:entE
Ordered Locus Names:BSU31980
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU31980. [Micado]

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5395392,3-dihydroxybenzoate-AMP ligasePRO_0000193074Add
BLAST

Proteomic databases

PaxDbiP40871.

Interactioni

Protein-protein interaction databases

IntActiP40871. 1 interaction.
MINTiMINT-8366125.
STRINGi224308.BSU31980.

Structurei

Secondary structure

1
539
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 189
Helixi27 – 3812
Beta strandi41 – 466
Beta strandi49 – 524
Helixi53 – 7018
Beta strandi77 – 804
Beta strandi84 – 863
Helixi87 – 9812
Beta strandi101 – 1044
Helixi111 – 12010
Beta strandi124 – 1329
Helixi137 – 14711
Beta strandi154 – 1585
Beta strandi163 – 1653
Helixi166 – 1683
Beta strandi183 – 1897
Beta strandi193 – 1964
Beta strandi199 – 2035
Helixi204 – 21815
Beta strandi225 – 2284
Helixi235 – 2395
Helixi242 – 2487
Beta strandi252 – 2554
Beta strandi257 – 2604
Helixi261 – 27111
Beta strandi274 – 2785
Helixi280 – 29213
Beta strandi302 – 3087
Helixi312 – 3154
Helixi318 – 3214
Beta strandi324 – 3318
Beta strandi337 – 3393
Beta strandi342 – 3443
Helixi346 – 3516
Beta strandi355 – 3584
Beta strandi362 – 3665
Beta strandi379 – 3846
Helixi396 – 4027
Beta strandi409 – 4179
Beta strandi419 – 4213
Beta strandi423 – 4286
Helixi429 – 4313
Beta strandi433 – 4353
Beta strandi438 – 4403
Helixi442 – 4498
Beta strandi455 – 46511
Turni466 – 4683
Beta strandi469 – 48113
Helixi485 – 49410
Helixi499 – 5013
Beta strandi504 – 5085
Helixi522 – 53413

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MD9X-ray2.80A1-539[»]
1MDBX-ray2.15A1-539[»]
1MDFX-ray2.50A1-539[»]
ProteinModelPortaliP40871.
SMRiP40871. Positions 1-536.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40871.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni234 – 2352Substrate binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1021.
HOGENOMiHOG000230011.
InParanoidiP40871.
KOiK02363.
OMAiMENELMG.
OrthoDBiEOG600DJW.
PhylomeDBiP40871.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR011963. DHB_AMP_lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02275. DHB_AMP_lig. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40871-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKGFTPWPD ELAETYRKNG CWAGETFGDL LRDRAAKYGD RIAITCGNTH
60 70 80 90 100
WSYRELDTRA DRLAAGFQKL GIQQMDRVVV QLPNIKEFFE VIFALFRLGA
110 120 130 140 150
LPVFALPSHR SSEITYFCEF AEAAAYIIPD AYSGFDYRSL ARQVQSKLPT
160 170 180 190 200
LKNIIVAGEA EEFLPLEDLH AEPVKLPEVK SSDVAFLQLS GGSTGLSKLI
210 220 230 240 250
PRTHDDYIYS LKRSVEVCWL DHSTVYLAAL PMAHNYPLSS PGVLGVLYAG
260 270 280 290 300
GRVVLSPSPS PDDAFPLIER EKVTITALVP PLAMVWMDAA SSRRDDLSSL
310 320 330 340 350
QVLQVGGAKF SAEAARRVKA VFGCTLQQVF GMAEGLVNYT RLDDPEEIIV
360 370 380 390 400
NTQGKPMSPY DEMRVWDDHD RDVKPGETGH LLTRGPYTIR GYYKAEEHNA
410 420 430 440 450
ASFTEDGFYR TGDIVRLTRD GYIVVEGRAK DQINRGGEKV AAEEVENHLL
460 470 480 490 500
AHPAVHDAAM VSMPDQFLGE RSCVFIIPRD EAPKAAELKA FLRERGLAAY
510 520 530
KIPDRVEFVE SFPQTGVGKV SKKALREAIS EKLLAGFKK
Length:539
Mass (Da):59,928
Last modified:November 1, 1995 - v2
Checksum:i027D286940B192F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti360 – 3601Y → I in AAA79759. (PubMed:8224884)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26444 Genomic DNA. Translation: AAC44632.1.
AL009126 Genomic DNA. Translation: CAB15188.1.
L08645 Genomic DNA. Translation: AAA79759.1.
PIRiD69615.
RefSeqiNP_391078.1. NC_000964.3.
WP_003243083.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB15188; CAB15188; BSU31980.
GeneIDi936509.
KEGGibsu:BSU31980.
PATRICi18978368. VBIBacSub10457_3347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26444 Genomic DNA. Translation: AAC44632.1 .
AL009126 Genomic DNA. Translation: CAB15188.1 .
L08645 Genomic DNA. Translation: AAA79759.1 .
PIRi D69615.
RefSeqi NP_391078.1. NC_000964.3.
WP_003243083.1. NZ_CM000487.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MD9 X-ray 2.80 A 1-539 [» ]
1MDB X-ray 2.15 A 1-539 [» ]
1MDF X-ray 2.50 A 1-539 [» ]
ProteinModelPortali P40871.
SMRi P40871. Positions 1-536.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P40871. 1 interaction.
MINTi MINT-8366125.
STRINGi 224308.BSU31980.

Proteomic databases

PaxDbi P40871.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15188 ; CAB15188 ; BSU31980 .
GeneIDi 936509.
KEGGi bsu:BSU31980.
PATRICi 18978368. VBIBacSub10457_3347.

Organism-specific databases

GenoListi BSU31980. [Micado ]

Phylogenomic databases

eggNOGi COG1021.
HOGENOMi HOG000230011.
InParanoidi P40871.
KOi K02363.
OMAi MENELMG.
OrthoDBi EOG600DJW.
PhylomeDBi P40871.

Enzyme and pathway databases

UniPathwayi UPA00013 .
BioCyci BSUB:BSU31980-MONOMER.
MetaCyc:MONOMER-13920.
SABIO-RK P40871.

Miscellaneous databases

EvolutionaryTracei P40871.

Family and domain databases

InterProi IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR011963. DHB_AMP_lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02275. DHB_AMP_lig. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and genetic organization of a Bacillus subtilis operon encoding 2,3-dihydroxybenzoate biosynthetic enzymes."
    Rowland B.M., Grossman T.H., Osburne M.S., Taber H.W.
    Gene 178:119-123(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Cloning and mapping of the Bacillus subtilis locus homologous to Escherichia coli ent genes."
    Adams R., Schumann W.
    Gene 133:119-121(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 341-533.
    Strain: 168.
  4. "Crystal structure of DhbE, an archetype for aryl acid activating domains of modular nonribosomal peptide synthetases."
    May J.J., Kessler N., Marahiel M.A., Stubbs M.T.
    Proc. Natl. Acad. Sci. U.S.A. 99:12120-12125(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEXES WITH AMP AND 2,3-DIHYDROXYBENZOATE.

Entry informationi

Entry nameiDHBE_BACSU
AccessioniPrimary (citable) accession number: P40871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3