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P40859 (MSOX_BACB0) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Monomeric sarcosine oxidase
Short name=MSOX
EC=1.5.3.1
Gene names
Name:soxA
Synonyms:sox
OrganismBacillus sp. (strain B-0618)
Taxonomic identifier69000 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidative demethylation of sarcosine. Can also oxidize other secondary amino acids such as N-methyl-L-alanine. HAMAP-Rule MF_00516

Catalytic activity

Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2. HAMAP-Rule MF_00516

Cofactor

Binds 1 FAD per subunit.

Enzyme regulation

Pyrrole-2-carboxylate is a competitive inhibitor. N-(cyclopropyl)glycine (CPG) is a mechanism-based inhibitor and inactivates the enzyme by covalently modifying the flavin. HAMAP-Rule MF_00516

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00516.

Sequence similarities

Belongs to the MSOX/MTOX family. MSOX subfamily.

Mass spectrometry

Molecular mass is 43839 Da from positions 1 - 390. Determined by ESI. Ref.3

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processtetrahydrofolate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsarcosine oxidase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Monomeric sarcosine oxidase HAMAP-Rule MF_00516
PRO_0000213762

Regions

Nucleotide binding6 – 3631FAD Potential

Amino acid modifications

Modified residue3161S-8alpha-FAD cysteine HAMAP-Rule MF_00516

Secondary structure

1 ............................................................................ 390
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40859 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: D830491B80230BCF

FASTA39043,182
        10         20         30         40         50         60 
MSTHFDVIVV GAGSMGMAAG YQLAKQGVKT LLVDAFDPPH TNGSHHGDTR IIRHAYGEGR 

        70         80         90        100        110        120 
EYVPLALRSQ ELWYELEKET HHKIFTKTGV LVFGPKGESA FVAETMEAAK EHSLTVDLLE 

       130        140        150        160        170        180 
GDEINKRWPG ITVPENYNAI FEPNSGVLFS ENCIRAYREL AEARGAKVLT HTRVEDFDIS 

       190        200        210        220        230        240 
PDSVKIETAN GSYTADKLIV SMGAWNSKLL SKLNLDIPLQ PYRQVVGFFE SDESKYSNDI 

       250        260        270        280        290        300 
DFPGFMVEVP NGIYYGFPSF GGCGLKLGYH TFGQKIDPDT INREFGVYPE DESNLRAFLE 

       310        320        330        340        350        360 
EYMPGANGEL KRGAVCMYTK TLDEHFIIDL HPEHSNVVIA AGFSGHGFKF SSGVGEVLSQ 

       370        380        390 
LALTGKTEHD ISIFSINRPA LKESLQKTTI

« Hide

References

[1]"Cloning, sequencing, overexpression in Escherichia coil of a sarcosine oxidase-encoding gene linked to the Bacillus creatinase gene."
Suzuki K., Sagai H., Imamura S., Sugiyama M.
J. Ferment. Bioeng. 77:231-234(1994)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Suzuki K.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 117.
[3]"Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase."
Wagner M.A., Khanna P., Jorns M.S.
Biochemistry 38:5588-5595(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 313-319, MASS SPECTROMETRY.
[4]"Monomeric sarcosine oxidase: 2. Kinetic studies with sarcosine, alternate substrates, and a substrate analogue."
Wagner M.A., Jorns M.S.
Biochemistry 39:8825-8829(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Inactivation of monomeric sarcosine oxidase by reaction with N-(cyclopropyl)glycine."
Zhao G., Qu J., Davis F.A., Jorns M.S.
Biochemistry 39:14341-14347(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme."
Trickey P., Wagner M.A., Jorns M.S., Mathews F.S.
Structure 7:331-345(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D16521 Genomic DNA. Translation: BAA03967.1.
PIRI39975.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EL5X-ray1.80A/B2-390[»]
1EL7X-ray1.90A/B2-390[»]
1EL8X-ray1.90A/B2-390[»]
1EL9X-ray2.00A/B2-390[»]
1ELIX-ray2.00A/B2-390[»]
1L9CX-ray1.90A/B2-390[»]
1L9DX-ray1.95A/B2-390[»]
1L9EX-ray1.85A/B2-390[»]
2A89X-ray1.85A/B2-390[»]
2GB0X-ray1.85A/B2-390[»]
2GF3X-ray1.30A/B2-390[»]
3BHFX-ray2.10A/B1-390[»]
3BHKX-ray1.71A/B1-390[»]
3M0OX-ray1.60A/B2-390[»]
3M12X-ray1.60A/B2-390[»]
3M13X-ray2.10A/B/C/D2-390[»]
3QSEX-ray1.75A/B2-390[»]
3QSMX-ray1.90A/B2-390[»]
3QSSX-ray1.85A/B2-390[»]
ProteinModelPortalP40859.
SMRP40859. Positions 2-390.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_00516. MSOX.
InterProIPR006076. FAD-dep_OxRdtase.
IPR006281. SoxA_mon.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
TIGRFAMsTIGR01377. soxA_mon. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP40859.

Entry information

Entry nameMSOX_BACB0
AccessionPrimary (citable) accession number: P40859
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: April 3, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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