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P40859

- MSOX_BACB0

UniProt

P40859 - MSOX_BACB0

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Protein

Monomeric sarcosine oxidase

Gene

soxA

Organism
Bacillus sp. (strain B-0618)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative demethylation of sarcosine. Can also oxidize other secondary amino acids such as N-methyl-L-alanine.

Catalytic activityi

Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.

Cofactori

FADNote: Binds 1 FAD per subunit.

Enzyme regulationi

Pyrrole-2-carboxylate is a competitive inhibitor. N-(cyclopropyl)glycine (CPG) is a mechanism-based inhibitor and inactivates the enzyme by covalently modifying the flavin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 3631FADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. sarcosine oxidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tetrahydrofolate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Monomeric sarcosine oxidase (EC:1.5.3.1)
Short name:
MSOX
Gene namesi
Name:soxA
Synonyms:sox
OrganismiBacillus sp. (strain B-0618)
Taxonomic identifieri69000 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Monomeric sarcosine oxidasePRO_0000213762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei316 – 3161S-8alpha-FAD cysteine

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
390
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106Combined sources
Helixi14 – 2512Combined sources
Beta strandi30 – 334Combined sources
Beta strandi41 – 455Combined sources
Beta strandi47 – 526Combined sources
Helixi60 – 623Combined sources
Helixi63 – 7917Combined sources
Beta strandi90 – 956Combined sources
Turni96 – 983Combined sources
Helixi100 – 11112Combined sources
Beta strandi117 – 1204Combined sources
Helixi122 – 1276Combined sources
Beta strandi137 – 1426Combined sources
Beta strandi146 – 1494Combined sources
Helixi150 – 16314Combined sources
Beta strandi167 – 1693Combined sources
Beta strandi174 – 1796Combined sources
Beta strandi184 – 1885Combined sources
Beta strandi191 – 20010Combined sources
Helixi203 – 2053Combined sources
Helixi206 – 2094Combined sources
Helixi210 – 2134Combined sources
Beta strandi220 – 22910Combined sources
Helixi233 – 2364Combined sources
Helixi238 – 2403Combined sources
Beta strandi244 – 2496Combined sources
Beta strandi252 – 2576Combined sources
Beta strandi265 – 2717Combined sources
Turni278 – 2803Combined sources
Beta strandi287 – 2893Combined sources
Helixi290 – 30213Combined sources
Helixi304 – 3063Combined sources
Beta strandi310 – 32011Combined sources
Beta strandi322 – 3243Combined sources
Beta strandi327 – 3315Combined sources
Beta strandi334 – 3418Combined sources
Helixi348 – 3503Combined sources
Helixi351 – 36414Combined sources
Helixi372 – 3743Combined sources
Helixi379 – 3813Combined sources
Beta strandi384 – 3863Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EL5X-ray1.80A/B2-390[»]
1EL7X-ray1.90A/B2-390[»]
1EL8X-ray1.90A/B2-390[»]
1EL9X-ray2.00A/B2-390[»]
1ELIX-ray2.00A/B2-390[»]
1L9CX-ray1.90A/B2-390[»]
1L9DX-ray1.95A/B2-390[»]
1L9EX-ray1.85A/B2-390[»]
2A89X-ray1.85A/B2-390[»]
2GB0X-ray1.85A/B2-390[»]
2GF3X-ray1.30A/B2-390[»]
3BHFX-ray2.10A/B1-390[»]
3BHKX-ray1.71A/B1-390[»]
3M0OX-ray1.60A/B2-390[»]
3M12X-ray1.60A/B2-390[»]
3M13X-ray2.10A/B/C/D2-382[»]
3QSEX-ray1.75A/B2-390[»]
3QSMX-ray1.90A/B2-390[»]
3QSSX-ray1.85A/B2-390[»]
ProteinModelPortaliP40859.
SMRiP40859. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40859.

Family & Domainsi

Sequence similaritiesi

Belongs to the MSOX/MTOX family. MSOX subfamily.Curated

Family and domain databases

HAMAPiMF_00516. MSOX.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR006281. SoxA_mon.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01377. soxA_mon. 1 hit.

Sequencei

Sequence statusi: Complete.

P40859-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTHFDVIVV GAGSMGMAAG YQLAKQGVKT LLVDAFDPPH TNGSHHGDTR
60 70 80 90 100
IIRHAYGEGR EYVPLALRSQ ELWYELEKET HHKIFTKTGV LVFGPKGESA
110 120 130 140 150
FVAETMEAAK EHSLTVDLLE GDEINKRWPG ITVPENYNAI FEPNSGVLFS
160 170 180 190 200
ENCIRAYREL AEARGAKVLT HTRVEDFDIS PDSVKIETAN GSYTADKLIV
210 220 230 240 250
SMGAWNSKLL SKLNLDIPLQ PYRQVVGFFE SDESKYSNDI DFPGFMVEVP
260 270 280 290 300
NGIYYGFPSF GGCGLKLGYH TFGQKIDPDT INREFGVYPE DESNLRAFLE
310 320 330 340 350
EYMPGANGEL KRGAVCMYTK TLDEHFIIDL HPEHSNVVIA AGFSGHGFKF
360 370 380 390
SSGVGEVLSQ LALTGKTEHD ISIFSINRPA LKESLQKTTI
Length:390
Mass (Da):43,182
Last modified:February 1, 1996 - v2
Checksum:iD830491B80230BCF
GO

Mass spectrometryi

Molecular mass is 43839 Da from positions 1 - 390. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16521 Genomic DNA. Translation: BAA03967.1.
PIRiI39975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16521 Genomic DNA. Translation: BAA03967.1 .
PIRi I39975.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EL5 X-ray 1.80 A/B 2-390 [» ]
1EL7 X-ray 1.90 A/B 2-390 [» ]
1EL8 X-ray 1.90 A/B 2-390 [» ]
1EL9 X-ray 2.00 A/B 2-390 [» ]
1ELI X-ray 2.00 A/B 2-390 [» ]
1L9C X-ray 1.90 A/B 2-390 [» ]
1L9D X-ray 1.95 A/B 2-390 [» ]
1L9E X-ray 1.85 A/B 2-390 [» ]
2A89 X-ray 1.85 A/B 2-390 [» ]
2GB0 X-ray 1.85 A/B 2-390 [» ]
2GF3 X-ray 1.30 A/B 2-390 [» ]
3BHF X-ray 2.10 A/B 1-390 [» ]
3BHK X-ray 1.71 A/B 1-390 [» ]
3M0O X-ray 1.60 A/B 2-390 [» ]
3M12 X-ray 1.60 A/B 2-390 [» ]
3M13 X-ray 2.10 A/B/C/D 2-382 [» ]
3QSE X-ray 1.75 A/B 2-390 [» ]
3QSM X-ray 1.90 A/B 2-390 [» ]
3QSS X-ray 1.85 A/B 2-390 [» ]
ProteinModelPortali P40859.
SMRi P40859. Positions 2-390.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB03147. Flavin adenine dinucleotide.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P40859.

Family and domain databases

HAMAPi MF_00516. MSOX.
InterProi IPR006076. FAD-dep_OxRdtase.
IPR006281. SoxA_mon.
[Graphical view ]
Pfami PF01266. DAO. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01377. soxA_mon. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequencing, overexpression in Escherichia coil of a sarcosine oxidase-encoding gene linked to the Bacillus creatinase gene."
    Suzuki K., Sagai H., Imamura S., Sugiyama M.
    J. Ferment. Bioeng. 77:231-234(1994)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Suzuki K.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 117.
  3. "Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase."
    Wagner M.A., Khanna P., Jorns M.S.
    Biochemistry 38:5588-5595(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 313-319, MASS SPECTROMETRY.
  4. "Monomeric sarcosine oxidase: 2. Kinetic studies with sarcosine, alternate substrates, and a substrate analogue."
    Wagner M.A., Jorns M.S.
    Biochemistry 39:8825-8829(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Inactivation of monomeric sarcosine oxidase by reaction with N-(cyclopropyl)glycine."
    Zhao G., Qu J., Davis F.A., Jorns M.S.
    Biochemistry 39:14341-14347(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme."
    Trickey P., Wagner M.A., Jorns M.S., Mathews F.S.
    Structure 7:331-345(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiMSOX_BACB0
AccessioniPrimary (citable) accession number: P40859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3