Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P40859

- MSOX_BACB0

UniProt

P40859 - MSOX_BACB0

Protein

Monomeric sarcosine oxidase

Gene

soxA

Organism
Bacillus sp. (strain B-0618)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the oxidative demethylation of sarcosine. Can also oxidize other secondary amino acids such as N-methyl-L-alanine.

    Catalytic activityi

    Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.

    Cofactori

    Binds 1 FAD per subunit.

    Enzyme regulationi

    Pyrrole-2-carboxylate is a competitive inhibitor. N-(cyclopropyl)glycine (CPG) is a mechanism-based inhibitor and inactivates the enzyme by covalently modifying the flavin.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi6 – 3631FADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. sarcosine oxidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tetrahydrofolate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monomeric sarcosine oxidase (EC:1.5.3.1)
    Short name:
    MSOX
    Gene namesi
    Name:soxA
    Synonyms:sox
    OrganismiBacillus sp. (strain B-0618)
    Taxonomic identifieri69000 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Monomeric sarcosine oxidasePRO_0000213762Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei316 – 3161S-8alpha-FAD cysteine

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    390
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 106
    Helixi14 – 2512
    Beta strandi30 – 334
    Beta strandi41 – 455
    Beta strandi47 – 526
    Helixi60 – 623
    Helixi63 – 7917
    Beta strandi90 – 956
    Turni96 – 983
    Helixi100 – 11112
    Beta strandi117 – 1204
    Helixi122 – 1276
    Beta strandi137 – 1426
    Beta strandi146 – 1494
    Helixi150 – 16314
    Beta strandi167 – 1693
    Beta strandi174 – 1796
    Beta strandi184 – 1885
    Beta strandi191 – 20010
    Helixi203 – 2053
    Helixi206 – 2094
    Helixi210 – 2134
    Beta strandi220 – 22910
    Helixi233 – 2364
    Helixi238 – 2403
    Beta strandi244 – 2496
    Beta strandi252 – 2576
    Beta strandi265 – 2717
    Turni278 – 2803
    Beta strandi287 – 2893
    Helixi290 – 30213
    Helixi304 – 3063
    Beta strandi310 – 32011
    Beta strandi322 – 3243
    Beta strandi327 – 3315
    Beta strandi334 – 3418
    Helixi348 – 3503
    Helixi351 – 36414
    Helixi372 – 3743
    Helixi379 – 3813
    Beta strandi384 – 3863

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EL5X-ray1.80A/B2-390[»]
    1EL7X-ray1.90A/B2-390[»]
    1EL8X-ray1.90A/B2-390[»]
    1EL9X-ray2.00A/B2-390[»]
    1ELIX-ray2.00A/B2-390[»]
    1L9CX-ray1.90A/B2-390[»]
    1L9DX-ray1.95A/B2-390[»]
    1L9EX-ray1.85A/B2-390[»]
    2A89X-ray1.85A/B2-390[»]
    2GB0X-ray1.85A/B2-390[»]
    2GF3X-ray1.30A/B2-390[»]
    3BHFX-ray2.10A/B1-390[»]
    3BHKX-ray1.71A/B1-390[»]
    3M0OX-ray1.60A/B2-390[»]
    3M12X-ray1.60A/B2-390[»]
    3M13X-ray2.10A/B/C/D2-382[»]
    3QSEX-ray1.75A/B2-390[»]
    3QSMX-ray1.90A/B2-390[»]
    3QSSX-ray1.85A/B2-390[»]
    ProteinModelPortaliP40859.
    SMRiP40859. Positions 2-390.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40859.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MSOX/MTOX family. MSOX subfamily.Curated

    Family and domain databases

    HAMAPiMF_00516. MSOX.
    InterProiIPR006076. FAD-dep_OxRdtase.
    IPR006281. SoxA_mon.
    [Graphical view]
    PfamiPF01266. DAO. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01377. soxA_mon. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40859-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTHFDVIVV GAGSMGMAAG YQLAKQGVKT LLVDAFDPPH TNGSHHGDTR    50
    IIRHAYGEGR EYVPLALRSQ ELWYELEKET HHKIFTKTGV LVFGPKGESA 100
    FVAETMEAAK EHSLTVDLLE GDEINKRWPG ITVPENYNAI FEPNSGVLFS 150
    ENCIRAYREL AEARGAKVLT HTRVEDFDIS PDSVKIETAN GSYTADKLIV 200
    SMGAWNSKLL SKLNLDIPLQ PYRQVVGFFE SDESKYSNDI DFPGFMVEVP 250
    NGIYYGFPSF GGCGLKLGYH TFGQKIDPDT INREFGVYPE DESNLRAFLE 300
    EYMPGANGEL KRGAVCMYTK TLDEHFIIDL HPEHSNVVIA AGFSGHGFKF 350
    SSGVGEVLSQ LALTGKTEHD ISIFSINRPA LKESLQKTTI 390
    Length:390
    Mass (Da):43,182
    Last modified:February 1, 1996 - v2
    Checksum:iD830491B80230BCF
    GO

    Mass spectrometryi

    Molecular mass is 43839 Da from positions 1 - 390. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16521 Genomic DNA. Translation: BAA03967.1.
    PIRiI39975.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16521 Genomic DNA. Translation: BAA03967.1 .
    PIRi I39975.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EL5 X-ray 1.80 A/B 2-390 [» ]
    1EL7 X-ray 1.90 A/B 2-390 [» ]
    1EL8 X-ray 1.90 A/B 2-390 [» ]
    1EL9 X-ray 2.00 A/B 2-390 [» ]
    1ELI X-ray 2.00 A/B 2-390 [» ]
    1L9C X-ray 1.90 A/B 2-390 [» ]
    1L9D X-ray 1.95 A/B 2-390 [» ]
    1L9E X-ray 1.85 A/B 2-390 [» ]
    2A89 X-ray 1.85 A/B 2-390 [» ]
    2GB0 X-ray 1.85 A/B 2-390 [» ]
    2GF3 X-ray 1.30 A/B 2-390 [» ]
    3BHF X-ray 2.10 A/B 1-390 [» ]
    3BHK X-ray 1.71 A/B 1-390 [» ]
    3M0O X-ray 1.60 A/B 2-390 [» ]
    3M12 X-ray 1.60 A/B 2-390 [» ]
    3M13 X-ray 2.10 A/B/C/D 2-382 [» ]
    3QSE X-ray 1.75 A/B 2-390 [» ]
    3QSM X-ray 1.90 A/B 2-390 [» ]
    3QSS X-ray 1.85 A/B 2-390 [» ]
    ProteinModelPortali P40859.
    SMRi P40859. Positions 2-390.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P40859.

    Family and domain databases

    HAMAPi MF_00516. MSOX.
    InterProi IPR006076. FAD-dep_OxRdtase.
    IPR006281. SoxA_mon.
    [Graphical view ]
    Pfami PF01266. DAO. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01377. soxA_mon. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, overexpression in Escherichia coil of a sarcosine oxidase-encoding gene linked to the Bacillus creatinase gene."
      Suzuki K., Sagai H., Imamura S., Sugiyama M.
      J. Ferment. Bioeng. 77:231-234(1994)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Suzuki K.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 117.
    3. "Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase."
      Wagner M.A., Khanna P., Jorns M.S.
      Biochemistry 38:5588-5595(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 313-319, MASS SPECTROMETRY.
    4. "Monomeric sarcosine oxidase: 2. Kinetic studies with sarcosine, alternate substrates, and a substrate analogue."
      Wagner M.A., Jorns M.S.
      Biochemistry 39:8825-8829(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Inactivation of monomeric sarcosine oxidase by reaction with N-(cyclopropyl)glycine."
      Zhao G., Qu J., Davis F.A., Jorns M.S.
      Biochemistry 39:14341-14347(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme."
      Trickey P., Wagner M.A., Jorns M.S., Mathews F.S.
      Structure 7:331-345(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiMSOX_BACB0
    AccessioniPrimary (citable) accession number: P40859
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3