Very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase PHS1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40857 (PHS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase PHS1
EC=4.2.1.134

Alternative name(s):
3-hydroxyacyl-CoA dehydratase PHS1
Short name=HACD
PTPLA homolog involved in sphingolipid biosynthesis protein 1

Gene names

Name:	PHS1
Ordered Locus Names:	YJL097W
ORF Names:	J0902

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the dehydration step in very long-chain fatty acid (VLCFA) synthesis. Ref.7 Ref.8
Catalytic activity	A very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] = a very-long-chain trans-2,3-dehydroacyl-[acyl-carrier protein] + H₂O. Ref.7 Ref.8
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein Ref.5 Ref.8.
Sequence similarities	Belongs to the very long-chain fatty acids dehydratase HACD family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW sphingolipid biosynthetic process Inferred from mutant phenotype Ref.8. Source: SGD vacuolar transport Inferred from mutant phenotype PubMed 16943325. Source: SGD
Cellular_component	fungal-type vacuole membrane Inferred from direct assay PubMed 14690591. Source: SGD integral to endoplasmic reticulum membrane Inferred from direct assay Ref.8. Source: SGD
Molecular_function	enoyl-CoA hydratase activity Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 217

217

Very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase PHS1

PRO_0000203047

Regions

Topological domain

1 – 11

Cytoplasmic Potential

Transmembrane

12 – 29

Helical; Potential

Topological domain

30 – 47

Lumenal Potential

Transmembrane

48 – 66

Helical; Potential

Topological domain

67 – 76

Cytoplasmic Potential

Transmembrane

77 – 94

Helical; Potential

Topological domain

95 – 99

Lumenal Potential

Transmembrane

100 – 117

Helical; Potential

Topological domain

118 – 142

Cytoplasmic Potential

Transmembrane

143 – 160

Helical; Potential

Topological domain

161 – 178

Lumenal Potential

Transmembrane

179 – 196

Helical; Potential

Topological domain

197 – 217

Cytoplasmic Potential

Motif

214 – 217

Endoplasmic reticulum retention signal

Sites

Active site

149

Probable

Active site

156

Probable

Experimental info

Mutagenesis

149

Y → A: No catalytic activity. Ref.8

Mutagenesis

156

E → A: No catalytic activity. Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P40857 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5C87BC9563FB83AA
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FASTA

217

24,511

        10         20         30         40         50         60 
MSKKLASPLS FLPLYNLLSA VGWSYLLYLV ISLYPKVGQP AFFYQTKNVA TLVQCGAIIE 

        70         80         90        100        110        120 
IINSFLGVVR SPLLTTVAQV SSRLLVVLGI FQLLPNTSGV QSVVYISLLL AWSITEIVRY 

       130        140        150        160        170        180 
LYYFFMLVFK NGAPKILILL RYNLFWILYP TGVASELRII YCALNAAESQ YSLLYKRILI 

       190        200        210 
AAMLAYIPGF PMLFLHMVAQ RKKVMKSLRS SFGKKLI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene." Miosga T., Boles E., Schaaff-Gerstenschlaeger I., Schmitt S., Zimmermann F.K. Yeast 10:1481-1488(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"A molecular caliper mechanism for determining very long-chain fatty acid length." Denic V., Weissman J.S. Cell 130:663-677(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[8]	"Membrane topology and essential amino acid residues of Phs1, a 3-hydroxyacyl-CoA dehydratase involved in very long-chain fatty acid elongation." Kihara A., Sakuraba H., Ikeda M., Denpoh A., Igarashi Y. J. Biol. Chem. 283:11199-11209(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF TYR-149 AND GLU-156, ACTIVE SITE, CATALYTIC ACTIVITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77923 Genomic DNA. Translation: CAA54893.1. Z49372 Genomic DNA. Translation: CAA89391.1. AY557855 Genomic DNA. Translation: AAS56181.1. BK006943 Genomic DNA. Translation: DAA08703.1.
PIR	S50296.
RefSeq	NP_012438.1. NM_001181530.1.
3D structure databases
ProteinModelPortal	P40857.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2104N.
IntAct	P40857. 31 interactions.
MINT	MINT-534987.
STRING	4932.YJL097W.
Proteomic databases
PaxDb	P40857.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL097W; YJL097W; YJL097W.
GeneID	853348.
KEGG	sce:YJL097W.
Organism-specific databases
CYGD	YJL097w.
SGD	S000003633. PHS1.
Phylogenomic databases
eggNOG	COG5198.
GeneTree	ENSGT00530000062962.
HOGENOM	HOG000190538.
KO	K10703.
OMA	WAITEII.
OrthoDB	EOG49CTJ4.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-17327.
Gene expression databases
Genevestigator	P40857.
GermOnline	YJL097W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007482. Tyr_Pase-like_PTPLA. [Graphical view]
PANTHER	PTHR11035. PTHR11035. 1 hit.
Pfam	PF04387. PTPLA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973747.

Entry information

Entry name

PHS1_YEAST

Accession

Primary (citable) accession number: P40857
Secondary accession number(s): D6VW87

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents