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P40857

- PHS1_YEAST

UniProt

P40857 - PHS1_YEAST

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Protein
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PHS1
Gene
PHS1, YJL097W, J0902
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for the dehydration step in very long-chain fatty acid (VLCFA) synthesis.2 Publications

Catalytic activityi

A very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain trans-2,3-dehydroacyl-CoA + H2O.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491 Inferred
Active sitei156 – 1561 Inferred

GO - Molecular functioni

  1. enoyl-CoA hydratase activity Source: SGD

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
  2. sphingolipid biosynthetic process Source: SGD
  3. vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17327.
YEAST:G3O-31552-MONOMER.
YEAST:MONOMER3O-85.

Names & Taxonomyi

Protein namesi
Recommended name:
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PHS1 (EC:4.2.1.134)
Alternative name(s):
3-hydroxyacyl-CoA dehydratase PHS1
Short name:
HACD
PTPLA homolog involved in sphingolipid biosynthesis protein 1
Gene namesi
Name:PHS1
Ordered Locus Names:YJL097W
ORF Names:J0902
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL097w.
SGDiS000003633. PHS1.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei12 – 2918Helical; Reviewed prediction
Add
BLAST
Topological domaini30 – 4718Lumenal Reviewed prediction
Add
BLAST
Transmembranei48 – 6619Helical; Reviewed prediction
Add
BLAST
Topological domaini67 – 7610Cytoplasmic Reviewed prediction
Transmembranei77 – 9418Helical; Reviewed prediction
Add
BLAST
Topological domaini95 – 995Lumenal Reviewed prediction
Transmembranei100 – 11718Helical; Reviewed prediction
Add
BLAST
Topological domaini118 – 14225Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei143 – 16018Helical; Reviewed prediction
Add
BLAST
Topological domaini161 – 17818Lumenal Reviewed prediction
Add
BLAST
Transmembranei179 – 19618Helical; Reviewed prediction
Add
BLAST
Topological domaini197 – 21721Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
  2. integral component of endoplasmic reticulum membrane Source: SGD
  3. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi149 – 1491Y → A: No catalytic activity. 1 Publication
Mutagenesisi156 – 1561E → A: No catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PHS1
PRO_0000203047Add
BLAST

Proteomic databases

PaxDbiP40857.

Expressioni

Gene expression databases

GenevestigatoriP40857.

Interactioni

Protein-protein interaction databases

BioGridi33660. 27 interactions.
DIPiDIP-2104N.
IntActiP40857. 31 interactions.
MINTiMINT-534987.
STRINGi4932.YJL097W.

Structurei

3D structure databases

ProteinModelPortaliP40857.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi214 – 2174Endoplasmic reticulum retention signal

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5198.
GeneTreeiENSGT00530000062962.
HOGENOMiHOG000190538.
KOiK10703.
OMAiGEFTKWT.
OrthoDBiEOG7NKKXC.

Family and domain databases

InterProiIPR007482. Tyr_Pase-like_PTPLA.
[Graphical view]
PANTHERiPTHR11035. PTHR11035. 1 hit.
PfamiPF04387. PTPLA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40857-1 [UniParc]FASTAAdd to Basket

« Hide

MSKKLASPLS FLPLYNLLSA VGWSYLLYLV ISLYPKVGQP AFFYQTKNVA    50
TLVQCGAIIE IINSFLGVVR SPLLTTVAQV SSRLLVVLGI FQLLPNTSGV 100
QSVVYISLLL AWSITEIVRY LYYFFMLVFK NGAPKILILL RYNLFWILYP 150
TGVASELRII YCALNAAESQ YSLLYKRILI AAMLAYIPGF PMLFLHMVAQ 200
RKKVMKSLRS SFGKKLI 217
Length:217
Mass (Da):24,511
Last modified:February 1, 1995 - v1
Checksum:i5C87BC9563FB83AA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77923 Genomic DNA. Translation: CAA54893.1.
Z49372 Genomic DNA. Translation: CAA89391.1.
AY557855 Genomic DNA. Translation: AAS56181.1.
BK006943 Genomic DNA. Translation: DAA08703.1.
PIRiS50296.
RefSeqiNP_012438.1. NM_001181530.1.

Genome annotation databases

EnsemblFungiiYJL097W; YJL097W; YJL097W.
GeneIDi853348.
KEGGisce:YJL097W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77923 Genomic DNA. Translation: CAA54893.1 .
Z49372 Genomic DNA. Translation: CAA89391.1 .
AY557855 Genomic DNA. Translation: AAS56181.1 .
BK006943 Genomic DNA. Translation: DAA08703.1 .
PIRi S50296.
RefSeqi NP_012438.1. NM_001181530.1.

3D structure databases

ProteinModelPortali P40857.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33660. 27 interactions.
DIPi DIP-2104N.
IntActi P40857. 31 interactions.
MINTi MINT-534987.
STRINGi 4932.YJL097W.

Proteomic databases

PaxDbi P40857.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL097W ; YJL097W ; YJL097W .
GeneIDi 853348.
KEGGi sce:YJL097W.

Organism-specific databases

CYGDi YJL097w.
SGDi S000003633. PHS1.

Phylogenomic databases

eggNOGi COG5198.
GeneTreei ENSGT00530000062962.
HOGENOMi HOG000190538.
KOi K10703.
OMAi GEFTKWT.
OrthoDBi EOG7NKKXC.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17327.
YEAST:G3O-31552-MONOMER.
YEAST:MONOMER3O-85.

Miscellaneous databases

NextBioi 973747.

Gene expression databases

Genevestigatori P40857.

Family and domain databases

InterProi IPR007482. Tyr_Pase-like_PTPLA.
[Graphical view ]
PANTHERi PTHR11035. PTHR11035. 1 hit.
Pfami PF04387. PTPLA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene."
    Miosga T., Boles E., Schaaff-Gerstenschlaeger I., Schmitt S., Zimmermann F.K.
    Yeast 10:1481-1488(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "A molecular caliper mechanism for determining very long-chain fatty acid length."
    Denic V., Weissman J.S.
    Cell 130:663-677(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  8. "Membrane topology and essential amino acid residues of Phs1, a 3-hydroxyacyl-CoA dehydratase involved in very long-chain fatty acid elongation."
    Kihara A., Sakuraba H., Ikeda M., Denpoh A., Igarashi Y.
    J. Biol. Chem. 283:11199-11209(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF TYR-149 AND GLU-156, ACTIVE SITE, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiPHS1_YEAST
AccessioniPrimary (citable) accession number: P40857
Secondary accession number(s): D6VW87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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