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Protein

SIT4-associating protein SAP185

Gene

SAP185

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Associates with the SIT4 phosphatase in a cell cycle dependent manner. May be directly or indirectly involved in SIT4-dependent functions in budding and in normal G1 cyclin expression.

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • G1/S transition of mitotic cell cycle Source: SGD
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-31553-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SIT4-associating protein SAP185
Gene namesi
Name:SAP185
Ordered Locus Names:YJL098W
ORF Names:J0840
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL098W.
SGDiS000003634. SAP185.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058SIT4-associating protein SAP185PRO_0000046106Add
BLAST

Post-translational modificationi

Hyperphosphorylated in the absence of SIT4.

Proteomic databases

MaxQBiP40856.
PeptideAtlasiP40856.
PRIDEiP40856.

PTM databases

iPTMnetiP40856.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SIT4P206048EBI-16384,EBI-13707

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi33659. 132 interactions.
DIPiDIP-5851N.
IntActiP40856. 53 interactions.
MINTiMINT-643271.

Structurei

3D structure databases

ProteinModelPortaliP40856.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SAPS family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009899.
HOGENOMiHOG000248046.
InParanoidiP40856.
KOiK15458.
OMAiHIDNPPL.
OrthoDBiEOG7647CP.

Family and domain databases

InterProiIPR007587. SAPS.
[Graphical view]
PANTHERiPTHR12634. PTHR12634. 4 hits.
PfamiPF04499. SAPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSFWKFGQ DFGSQSPLAK LLNRAFIKID DKPTSTEAGK IDSNSTDESL
60 70 80 90 100
ESNSFKSEDE EEEYELPNRE EDYKAYKPNL SLLNDLLDDE ELYTELMCSN
110 120 130 140 150
FKLLVYLKYP EVLSKLIDYV RNSTILESNI DRVTSEDRDL VRGEDKDTTE
160 170 180 190 200
DFENAKADKK NIDGTFEEKE RTRSGEEEEL ENEENDSASE DTRVTLPHEL
210 220 230 240 250
EEHDDTRRAR IAAEILSADV WPISSALIEN EGLLAKLWSI LRLPSPLSIE
260 270 280 290 300
ASTYFMKINE RLLDMNMDGI IEFILKKEHI VDDFLAHIDN PPLMDFLLKV
310 320 330 340 350
ISTDKPEISN GVIQLFKKQN LVPKLIHLLD PVFDSCTQSA AGDFLKALVT
360 370 380 390 400
ISGNCPNEIT SSIGPNELTR QLVSPNMMKQ LMDIMLKGGN SLNNGVGIII
410 420 430 440 450
ELIRKNNSDY DTIQTNYTTI ESHPPTDRDP IYLGYLVKMF SEHMADFNKI
460 470 480 490 500
LTEKKIPLLQ TSYGTIEPLG FERFKICELI AELLHCSNMT LLNEPSAYDI
510 520 530 540 550
VRERDAERER IFNSQNYVDS NDRSELKENE DDNTGDADDE VEDDTNQVES
560 570 580 590 600
ANTSIDGEEV IDKLNSLQIE TNKVNQNMNN EEQHSLMPDF NNGDFKDEED
610 620 630 640 650
ENPFEPQYSD VILDSSDIEK NFRVSPNVGD QLKISLQDTR VIDTMLEMFF
660 670 680 690 700
HFQWNNFLHN VVYDVVQQIF NGPLKIGYNR FLLDDLLINI RLTDMIINGN
710 720 730 740 750
NECIEYEKGH DTRLGYMGHL TLIAEEVTKF TAYIEEMNIT FENTEVMSSL
760 770 780 790 800
FESKWIAYTE DVLEDLKEKY NAILGDIAEE GDMLQDEEED AVYDKGERTM
810 820 830 840 850
GTVDDYINDI MQMDNVRCQE EEEDEGEGYV SFDEDEPQEY RNGDSVRSKE
860 870 880 890 900
SNSSEGKRDQ EQLYYEYVNE DGTKTRLNFN PDSDATEQVP GEVNRDHKIP
910 920 930 940 950
LKLKRSFTDA CKSETIPNNT VNAKEESVFQ FSNELSDGWE SSPSNSIPKR
960 970 980 990 1000
ASPSKNGMNS PMFQHQFELH SPTDEFGGHK DEILSAEGHD YDIDEYDELS
1010 1020 1030 1040 1050
DDSDEEYDNC EDEDSLDYAD SAAYALCRSK SKDKISWDEE EQARLMGVVK

FNSEHYRD
Length:1,058
Mass (Da):121,403
Last modified:February 1, 1995 - v1
Checksum:iDD44DD53DDD87438
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77923 Genomic DNA. Translation: CAA54892.1.
X85021 Genomic DNA. Translation: CAA59396.1.
Z49373 Genomic DNA. Translation: CAA89392.1.
BK006943 Genomic DNA. Translation: DAA08702.1.
PIRiS50295.
RefSeqiNP_012437.1. NM_001181531.1.

Genome annotation databases

EnsemblFungiiYJL098W; YJL098W; YJL098W.
GeneIDi853347.
KEGGisce:YJL098W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77923 Genomic DNA. Translation: CAA54892.1.
X85021 Genomic DNA. Translation: CAA59396.1.
Z49373 Genomic DNA. Translation: CAA89392.1.
BK006943 Genomic DNA. Translation: DAA08702.1.
PIRiS50295.
RefSeqiNP_012437.1. NM_001181531.1.

3D structure databases

ProteinModelPortaliP40856.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33659. 132 interactions.
DIPiDIP-5851N.
IntActiP40856. 53 interactions.
MINTiMINT-643271.

PTM databases

iPTMnetiP40856.

Proteomic databases

MaxQBiP40856.
PeptideAtlasiP40856.
PRIDEiP40856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL098W; YJL098W; YJL098W.
GeneIDi853347.
KEGGisce:YJL098W.

Organism-specific databases

EuPathDBiFungiDB:YJL098W.
SGDiS000003634. SAP185.

Phylogenomic databases

GeneTreeiENSGT00390000009899.
HOGENOMiHOG000248046.
InParanoidiP40856.
KOiK15458.
OMAiHIDNPPL.
OrthoDBiEOG7647CP.

Enzyme and pathway databases

BioCyciYEAST:G3O-31553-MONOMER.

Miscellaneous databases

NextBioi973744.
PROiP40856.

Family and domain databases

InterProiIPR007587. SAPS.
[Graphical view]
PANTHERiPTHR12634. PTHR12634. 4 hits.
PfamiPF04499. SAPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The SAPs, a new family of proteins, associate and function positively with the SIT4 phosphatase."
    Luke M.M., della Seta F., di Como C.J., Sugimoto H., Kobayashi R., Arndt K.T.
    Mol. Cell. Biol. 16:2744-2755(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene."
    Miosga T., Boles E., Schaaff-Gerstenschlaeger I., Schmitt S., Zimmermann F.K.
    Yeast 10:1481-1488(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
    Rasmussen S.W.
    Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-853.
    Strain: ATCC 96604 / S288c / FY1679.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSA185_YEAST
AccessioniPrimary (citable) accession number: P40856
Secondary accession number(s): D6VW86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.