Reviewed, UniProtKB/Swiss-Prot P40854 (MSOX_STRSB)

Names and origin

Protein names

Recommended name:
    Monomeric sarcosine oxidase
      Short name=MSOX
    EC=1.5.3.1

Gene names

Name:

soxA

Organism

Streptomyces sp. (strain KB210-8SY)

Taxonomic identifier

72592 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidative demethylation of sarcosine By similarity. HAMAP MF_00516
Catalytic activity	Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2. HAMAP MF_00516
Cofactor	Binds 1 FAD per subunit By similarity. HAMAP MF_00516
Subunit structure	Monomer By similarity. HAMAP MF_00516
Subcellular location	Cytoplasm By similarity HAMAP MF_00516.
Sequence similarities	Belongs to the MSOX/MTOX family. MSOX subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro sarcosine oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 389	388	Monomeric sarcosine oxidase HAMAP MF_00516		PRO_0000213764
Regions
	Nucleotide binding	6 – 36	31	FAD Potential
Amino acid modifications
	Modified residue	315	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40854-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: EFE480AF4C1B73E6	FASTA	389	42,263
	10 20 30 40 50 60 MSPTYDVIVI GLGGMGSAAA HHLSARGARV LGLEKFGPVH NRGSSHGGSR ITRQSYFEDP 70 80 90 100 110 120 AYVPLLLRAY ELYEELERAT GRNVATLCGG VMAGPPDSRT VSGSLRSATE WDLAHEMLDA 130 140 150 160 170 180 KEIRRRFPTL APDDDEVALF EAKAGLLRPE NMVAAHLQLA TRQGAELRFE EPVLRWEPYR 190 200 210 220 230 240 DGVRVHTGEN TYTAGQLVIC PGAWAPQLLA DIGVPITVER QIMYWFQPKG GTGPFVPERH 250 260 270 280 290 300 PVYIWEDADG VQVYGFPAID GPEKGAKVAF FRKGQHTTPE TIDRTVHAHE VRAMADHMSA 310 320 330 340 350 360 LIPDLPGTFL KAATCMYSNT PDEHFVIARH PAHPESVTVA CGFSGHGFKF VPVVGEILAD 370 380 LALTGATAHP IGLFDPARLT APAARGVQP

References

[1]

"Molecular cloning and expression of a Streptomyces sarcosine oxidase gene in Streptomyces lividans." Suzuki K., Ogishima M., Sugiyama M., Inouye Y., Nakamura S., Imamura S. Biosci. Biotechnol. Biochem. 56:432-436(1992) [PubMed: 1368326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31; 122-143; 230-259 AND 268-283.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S39464 Genomic DNA. Translation: AAC60412.2. D10623 Genomic DNA. Translation: BAA01473.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_00516. MSOX. [Tree]
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSOX_STRSB

Accession

Primary (citable) accession number: P40854

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)