P40851 (AXL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative protease AXL1
EC=3.4.24.-

Gene names

Name:	AXL1
Ordered Locus Names:	YPR122W
ORF Names:	P9642.4

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable protease. Involved in axial budding.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with BUD5. Ref.4
Subcellular location	Bud neck. Note: Mother-bud neck and division site remnants of haploid cells. Ref.4
Miscellaneous	Present with 623 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axial cellular bud site selection Inferred from mutant phenotype Ref.1. Source: SGD peptide pheromone maturation Inferred from mutant phenotype PubMed 7569998. Source: SGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cellular bud neck Inferred from direct assay Ref.4. Source: SGD mating projection tip Inferred from direct assay Ref.4. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from mutant phenotype PubMed 7569998. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1208	1208	Putative protease AXL1		PRO_0000074417
Sites
	Active site	71	1	Proton acceptor By similarity
	Metal binding	68	1	Zinc By similarity
	Metal binding	72	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
Amino acid modifications
	Modified residue	262	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	113	1	E → G in BAA04613. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40851 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: 4FC2F237CC3FD978	FASTA	1,208	138,332
	10 20 30 40 50 60 MSLREVTNYE VSFYIPLSYS NRTHKVCKLP NGILALIISD PTDTSSSCSL TVCTGSHNDP 70 80 90 100 110 120 KDIAGLAHLC EHMILSAGSK KYPDPGLFHT LIAKNNGSQN AFTTGEQTTF YFELPNTQNN 130 140 150 160 170 180 GEFTFESILD VFASFFKEPL FNPLLISKEI YAIQSEHEGN ISSTTKIFYH AARILANPDH 190 200 210 220 230 240 PFSRFSTGNI HSLSSIPQLK KIKLKSSLNT YFENNFFGEN ITLCIRGPQS VNILTKLALS 250 260 270 280 290 300 KFGDIKPKSA VKERSISIRT RSFRRSKSLK KRQDSSKNDY SDLKTFKILN TTWEKKYKNT 310 320 330 340 350 360 MCFQQFPECN SIFINSNKVP IMRLLFPVSD KNTRFTKDDI KIYSHLWCEL FGDESPGSLS 370 380 390 400 410 420 YYLASKGWLT GCFAFTSEFA IGDIGLILEL ELTNSGWENI KRITTIVLNR LLPSFYVMNI 430 440 450 460 470 480 DYLITFLKEQ NLIDLVSFLY QSSEDLPMEE CSKLSGILQD DLECLTPPNI FKGFKSLIEI 490 500 510 520 530 540 DDPNIEKYEN TKANIQWWTG QAIKFQNFLK SFMNHDNMRL LLLGNIKSGN IFDKMKNKSD 550 560 570 580 590 600 ICTDFFYEFE YYTANVHLAS DNKFHSNSSY EFNFPTGNLF LPDCVSDPLK LQQLFLECSL 610 620 630 640 650 660 KSKFATLRPQ IYSEPTRTKP QLVSENQNYE MWILKEDPNF ASDNKSVVSF EVLGLGIKPS 670 680 690 700 710 720 PEATIHLEVL AQALFIITSS FLYPALRIGY TYEIASSSKG NVTLRFTISG FPEGVFTIVK 730 740 750 760 770 780 TFVDTLKLIA TDPTFLSKDT LRKARILVRN KYKNASSDNC VKLASVGLLI VLEKYIWTLE 790 800 810 820 830 840 DRINALELTE LESFEKFCFL FWRNPKHLVL FMQGSLEYAD AINRYLNNNF TQHLKISNEG 850 860 870 880 890 900 SKPTIRLYPP PSTKDLDQGT NAFISYNGHQ DDPNNSIVYF IQTAQRDDIK NLTLTFLTEY 910 920 930 940 950 960 LFSLTLVPDL RNKKQIGYIV LGGLRVLTDT VGIHITVMSG SSGHNLETRI NEYLSYLQLQ 970 980 990 1000 1010 1020 VLNRFTEFDF RRILLEPFLN LLKQNSTKQF EGSAGPVDLL NEIVANVQNG DNYTLNNKQM 1030 1040 1050 1060 1070 1080 RQHRKVRNKI AEGRLNFQED HEMIDISFLQ KLTLKKYLAF FESKISIYSA QRSKLSIMIT 1090 1100 1110 1120 1130 1140 SPMAEKEIAS RKMFLQLEAF LKINGFAIKN EDLKKIVEHS KGNPILLVKN LFTYFRRRNE 1150 1160 1170 1180 1190 1200 VFKLGTVVLQ EILKIIGMNL KQRYGSILGF SSQDGEGQEI EKFWNNDTSP IVPLQELPEP NFFRKAAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes." Fujita A., Oka C., Arikawa Y., Katagai T., Tonouchi A., Kuhara S., Misumi Y. Nature 372:567-570(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44774 / DBY747.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Subcellular localization of Axl1, the cell type-specific regulator of polarity." Lord M., Inose F., Hiroko T., Hata T., Fujita A., Chant J. Curr. Biol. 12:1347-1352(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BUD5, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17787 Genomic DNA. Translation: BAA04613.1. U40828 Genomic DNA. Translation: AAB68063.1. BK006949 Genomic DNA. Translation: DAA11537.1.
PIR	S69015.
RefSeq	NP_015447.1. NM_001184219.1.
3D structure databases
ProteinModelPortal	P40851.
SMR	P40851. Positions 21-411.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4872N.
IntAct	P40851. 3 interactions.
MINT	MINT-501242.
STRING	4932.YPR122W.
Protein family/group databases
MEROPS	M16.007.
Proteomic databases
PaxDb	P40851.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR122W; YPR122W; YPR122W.
GeneID	856240.
KEGG	sce:YPR122W.
Organism-specific databases
SGD	S000006326. AXL1.
Phylogenomic databases
eggNOG	COG1025.
HOGENOM	HOG000001003.
KO	K01422.
OMA	TGSHNDP.
OrthoDB	EOG4ZKNVG.
Gene expression databases
Genevestigator	P40851.
GermOnline	YPR122W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.830.10. 5 hits.
InterPro	IPR011249. Metalloenz_LuxS/M16. IPR011237. Pept_M16_dom. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
SUPFAM	SSF63411. Metalloenz_metal-bd. 4 hits.
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981504.

Entry information

Entry name

AXL1_YEAST

Accession

Primary (citable) accession number: P40851
Secondary accession number(s): D6W4C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program