Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative protease AXL1

Gene

AXL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable protease. Involved in axial budding.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681ZincPROSITE-ProRule annotation
Active sitei71 – 711Proton acceptorPROSITE-ProRule annotation
Metal bindingi72 – 721ZincPROSITE-ProRule annotation
Metal bindingi156 – 1561ZincPROSITE-ProRule annotation

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

  • axial cellular bud site selection Source: SGD
  • cytogamy Source: SGD
  • peptide mating pheromone maturation involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34261-MONOMER.

Protein family/group databases

MEROPSiM16.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative protease AXL1 (EC:3.4.24.-)
Gene namesi
Name:AXL1
Ordered Locus Names:YPR122W
ORF Names:P9642.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR122W.
SGDiS000006326. AXL1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • mating projection tip Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12081208Putative protease AXL1PRO_0000074417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei262 – 2621PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40851.

PTM databases

iPTMnetiP40851.

Interactioni

Subunit structurei

Interacts with BUD5.1 Publication

Protein-protein interaction databases

BioGridi36291. 56 interactions.
DIPiDIP-4872N.
IntActiP40851. 2 interactions.
MINTiMINT-501242.

Structurei

3D structure databases

ProteinModelPortaliP40851.
SMRiP40851. Positions 21-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M16 family.Curated

Phylogenomic databases

HOGENOMiHOG000001003.
InParanoidiP40851.
KOiK01422.
OMAiTGSHNDP.
OrthoDBiEOG769ZSZ.

Family and domain databases

Gene3Di3.30.830.10. 5 hits.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
PfamiPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
SUPFAMiSSF63411. SSF63411. 5 hits.
PROSITEiPS00143. INSULINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLREVTNYE VSFYIPLSYS NRTHKVCKLP NGILALIISD PTDTSSSCSL
60 70 80 90 100
TVCTGSHNDP KDIAGLAHLC EHMILSAGSK KYPDPGLFHT LIAKNNGSQN
110 120 130 140 150
AFTTGEQTTF YFELPNTQNN GEFTFESILD VFASFFKEPL FNPLLISKEI
160 170 180 190 200
YAIQSEHEGN ISSTTKIFYH AARILANPDH PFSRFSTGNI HSLSSIPQLK
210 220 230 240 250
KIKLKSSLNT YFENNFFGEN ITLCIRGPQS VNILTKLALS KFGDIKPKSA
260 270 280 290 300
VKERSISIRT RSFRRSKSLK KRQDSSKNDY SDLKTFKILN TTWEKKYKNT
310 320 330 340 350
MCFQQFPECN SIFINSNKVP IMRLLFPVSD KNTRFTKDDI KIYSHLWCEL
360 370 380 390 400
FGDESPGSLS YYLASKGWLT GCFAFTSEFA IGDIGLILEL ELTNSGWENI
410 420 430 440 450
KRITTIVLNR LLPSFYVMNI DYLITFLKEQ NLIDLVSFLY QSSEDLPMEE
460 470 480 490 500
CSKLSGILQD DLECLTPPNI FKGFKSLIEI DDPNIEKYEN TKANIQWWTG
510 520 530 540 550
QAIKFQNFLK SFMNHDNMRL LLLGNIKSGN IFDKMKNKSD ICTDFFYEFE
560 570 580 590 600
YYTANVHLAS DNKFHSNSSY EFNFPTGNLF LPDCVSDPLK LQQLFLECSL
610 620 630 640 650
KSKFATLRPQ IYSEPTRTKP QLVSENQNYE MWILKEDPNF ASDNKSVVSF
660 670 680 690 700
EVLGLGIKPS PEATIHLEVL AQALFIITSS FLYPALRIGY TYEIASSSKG
710 720 730 740 750
NVTLRFTISG FPEGVFTIVK TFVDTLKLIA TDPTFLSKDT LRKARILVRN
760 770 780 790 800
KYKNASSDNC VKLASVGLLI VLEKYIWTLE DRINALELTE LESFEKFCFL
810 820 830 840 850
FWRNPKHLVL FMQGSLEYAD AINRYLNNNF TQHLKISNEG SKPTIRLYPP
860 870 880 890 900
PSTKDLDQGT NAFISYNGHQ DDPNNSIVYF IQTAQRDDIK NLTLTFLTEY
910 920 930 940 950
LFSLTLVPDL RNKKQIGYIV LGGLRVLTDT VGIHITVMSG SSGHNLETRI
960 970 980 990 1000
NEYLSYLQLQ VLNRFTEFDF RRILLEPFLN LLKQNSTKQF EGSAGPVDLL
1010 1020 1030 1040 1050
NEIVANVQNG DNYTLNNKQM RQHRKVRNKI AEGRLNFQED HEMIDISFLQ
1060 1070 1080 1090 1100
KLTLKKYLAF FESKISIYSA QRSKLSIMIT SPMAEKEIAS RKMFLQLEAF
1110 1120 1130 1140 1150
LKINGFAIKN EDLKKIVEHS KGNPILLVKN LFTYFRRRNE VFKLGTVVLQ
1160 1170 1180 1190 1200
EILKIIGMNL KQRYGSILGF SSQDGEGQEI EKFWNNDTSP IVPLQELPEP

NFFRKAAF
Length:1,208
Mass (Da):138,332
Last modified:October 5, 2010 - v2
Checksum:i4FC2F237CC3FD978
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131E → G in BAA04613 (PubMed:7990931).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17787 Genomic DNA. Translation: BAA04613.1.
U40828 Genomic DNA. Translation: AAB68063.1.
BK006949 Genomic DNA. Translation: DAA11537.1.
PIRiS69015.
RefSeqiNP_015447.1. NM_001184219.1.

Genome annotation databases

EnsemblFungiiYPR122W; YPR122W; YPR122W.
GeneIDi856240.
KEGGisce:YPR122W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17787 Genomic DNA. Translation: BAA04613.1.
U40828 Genomic DNA. Translation: AAB68063.1.
BK006949 Genomic DNA. Translation: DAA11537.1.
PIRiS69015.
RefSeqiNP_015447.1. NM_001184219.1.

3D structure databases

ProteinModelPortaliP40851.
SMRiP40851. Positions 21-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36291. 56 interactions.
DIPiDIP-4872N.
IntActiP40851. 2 interactions.
MINTiMINT-501242.

Protein family/group databases

MEROPSiM16.007.

PTM databases

iPTMnetiP40851.

Proteomic databases

MaxQBiP40851.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR122W; YPR122W; YPR122W.
GeneIDi856240.
KEGGisce:YPR122W.

Organism-specific databases

EuPathDBiFungiDB:YPR122W.
SGDiS000006326. AXL1.

Phylogenomic databases

HOGENOMiHOG000001003.
InParanoidiP40851.
KOiK01422.
OMAiTGSHNDP.
OrthoDBiEOG769ZSZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-34261-MONOMER.

Miscellaneous databases

PROiP40851.

Family and domain databases

Gene3Di3.30.830.10. 5 hits.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
PfamiPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
SUPFAMiSSF63411. SSF63411. 5 hits.
PROSITEiPS00143. INSULINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes."
    Fujita A., Oka C., Arikawa Y., Katagai T., Tonouchi A., Kuhara S., Misumi Y.
    Nature 372:567-570(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44774 / DBY747.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Subcellular localization of Axl1, the cell type-specific regulator of polarity."
    Lord M., Inose F., Hiroko T., Hata T., Fujita A., Chant J.
    Curr. Biol. 12:1347-1352(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BUD5, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAXL1_YEAST
AccessioniPrimary (citable) accession number: P40851
Secondary accession number(s): D6W4C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 5, 2010
Last modified: June 8, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.