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P40850

- MKT1_YEAST

UniProt

P40850 - MKT1_YEAST

Protein

Protein MKT1

Gene

MKT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Required for propagation of M2 dsRNA satellite at temperature above 30 degrees Celsius if an L-A RNA virus carrying NEX (N) is present in the cells.

    GO - Molecular functioni

    1. nuclease activity Source: SGD

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: SGD
    2. DNA repair Source: InterPro
    3. interspecies interaction between organisms Source: SGD
    4. nucleic acid phosphodiester bond hydrolysis Source: GOC

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33114-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein MKT1
    Gene namesi
    Name:MKT1
    Ordered Locus Names:YNL085W
    ORF Names:N2302
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL085w.
    SGDiS000005029. MKT1.

    Subcellular locationi

    GO - Cellular componenti

    1. polysome Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 830830Protein MKT1PRO_0000096495Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei358 – 3581Phosphoserine1 Publication
    Modified residuei362 – 3621Phosphoserine1 Publication
    Modified residuei371 – 3711Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40850.
    PaxDbiP40850.
    PeptideAtlasiP40850.

    Expressioni

    Gene expression databases

    GenevestigatoriP40850.

    Interactioni

    Protein-protein interaction databases

    BioGridi35738. 73 interactions.
    DIPiDIP-6530N.
    IntActiP40850. 14 interactions.
    MINTiMINT-642141.

    Structurei

    3D structure databases

    ProteinModelPortaliP40850.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG5366.
    HOGENOMiHOG000113598.
    OMAiARYYQVK.
    OrthoDBiEOG722JJ1.

    Family and domain databases

    Gene3Di3.40.50.1010. 2 hits.
    InterProiIPR022039. MKT1_C.
    IPR022040. MKT1_N.
    IPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    IPR006085. XPG_DNA_repair_N.
    [Graphical view]
    PfamiPF12246. MKT1_C. 1 hit.
    PF12247. MKT1_N. 1 hit.
    PF00867. XPG_I. 1 hit.
    PF00752. XPG_N. 1 hit.
    [Graphical view]
    SMARTiSM00484. XPGI. 1 hit.
    SM00485. XPGN. 1 hit.
    [Graphical view]
    SUPFAMiSSF88723. SSF88723. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P40850-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIKSLESFL FERGLVGSYA IEALNNCTLD IDVNHYVSRL LTNKREQYLD    50
    AIGGFPTSLK MYLESDLKIF KDFNITPIFV FNGGLTYNQL EASGHFTAAS 100
    ASASISSTTT SSSGTNATTR SNTESVLLQR SRGWTQWNNL ISSNQNSYID 150
    QPIQPQEPFR HNTTIDSKAY QNDLIAYFIE HGYMYQVAPY SSWFQLAYLL 200
    NSAYIDAIYG PTDCLMLDCV DRFILGMEFP NKEFRFIDRS RVMKDLGCTH 250
    EEFIDIAMAV GNDLQPTTLP PLQIYPVPQL FDIALEMVLN TGTNFYAYQL 300
    STTLQNDSKE NIQNYQRGIS ALRYMPVLKD TGKVELFVQE IVVSEEDSEK 350
    NNKDGKKSNL SSPSSASSSA SPATTVTKNA SEKLTYEKSS TKEVRKPRDI 400
    PNDVHDFIGQ MLPHEYYFYR SIGLVTGKLF DAIVTGVYPE EPPLGGGSST 450
    SYKKLVSKSV EIFKNKEINL LTQPINRYYQ IKQIKQVKWY AANEPTTLTN 500
    RMSPSMFETI NHLIVKTETS DEKEFSISEF ITTINGSSNM AKDFISEKVI 550
    FPNSVPIESK LNSPFNLLST NFLRLLVLLE FFTFDFKEKL LEPTRWGEVF 600
    LKLNELNIDS KYHESVIIFL VFLKCDVLKL DEEVQPPAPS ALSQATLRSY 650
    PEESLYVLLI TRVLTLFQVD QKPSNYHGPI DKKTLIFRDH LSFIKENLNE 700
    LFEAVLISSL TSGEFNRLSL DNFGWARKIV RYLPFKLDSP NTIMAMMWEF 750
    FLQKYLHNGN AKNDALSLVA TEFNTYKSTP NLDEQFVESH RFLLEISKVM 800
    QELNAAKLID ENVFKLFTKA VEFTTTALSS 830
    Length:830
    Mass (Da):94,495
    Last modified:February 1, 1996 - v2
    Checksum:iBD976D8387E4F708
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti809 – 83022IDENV…TALSS → TMKTCLNYH in AAC49470. (PubMed:7532890)CuratedAdd
    BLAST

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti30 – 301D → G in strain: 2574, ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.
    Natural varianti453 – 4531K → R in strain: ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09129 Genomic DNA. Translation: AAC49470.1.
    AF458969 Genomic DNA. Translation: AAM00519.1.
    AF458975 Genomic DNA. Translation: AAM00555.1.
    AF458977 Genomic DNA. Translation: AAM00567.1.
    AF458978 Genomic DNA. Translation: AAM00573.1.
    AF458980 Genomic DNA. Translation: AAM00585.1.
    AF458981 Genomic DNA. Translation: AAM00591.1.
    DQ116825 Genomic DNA. Translation: AAZ23277.1.
    DQ116826 Genomic DNA. Translation: AAZ23278.1.
    DQ116827 Genomic DNA. Translation: AAZ23279.1.
    DQ116828 Genomic DNA. Translation: AAZ23280.1.
    X89016 Genomic DNA. Translation: CAA61425.1.
    Z71361 Genomic DNA. Translation: CAA95961.1.
    Z71360 Genomic DNA. Translation: CAA95960.1.
    BK006947 Genomic DNA. Translation: DAA10460.1.
    PIRiS57537.
    RefSeqiNP_014314.3. NM_001182923.3.

    Genome annotation databases

    EnsemblFungiiYNL085W; YNL085W; YNL085W.
    GeneIDi855639.
    KEGGisce:YNL085W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09129 Genomic DNA. Translation: AAC49470.1 .
    AF458969 Genomic DNA. Translation: AAM00519.1 .
    AF458975 Genomic DNA. Translation: AAM00555.1 .
    AF458977 Genomic DNA. Translation: AAM00567.1 .
    AF458978 Genomic DNA. Translation: AAM00573.1 .
    AF458980 Genomic DNA. Translation: AAM00585.1 .
    AF458981 Genomic DNA. Translation: AAM00591.1 .
    DQ116825 Genomic DNA. Translation: AAZ23277.1 .
    DQ116826 Genomic DNA. Translation: AAZ23278.1 .
    DQ116827 Genomic DNA. Translation: AAZ23279.1 .
    DQ116828 Genomic DNA. Translation: AAZ23280.1 .
    X89016 Genomic DNA. Translation: CAA61425.1 .
    Z71361 Genomic DNA. Translation: CAA95961.1 .
    Z71360 Genomic DNA. Translation: CAA95960.1 .
    BK006947 Genomic DNA. Translation: DAA10460.1 .
    PIRi S57537.
    RefSeqi NP_014314.3. NM_001182923.3.

    3D structure databases

    ProteinModelPortali P40850.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35738. 73 interactions.
    DIPi DIP-6530N.
    IntActi P40850. 14 interactions.
    MINTi MINT-642141.

    Proteomic databases

    MaxQBi P40850.
    PaxDbi P40850.
    PeptideAtlasi P40850.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL085W ; YNL085W ; YNL085W .
    GeneIDi 855639.
    KEGGi sce:YNL085W.

    Organism-specific databases

    CYGDi YNL085w.
    SGDi S000005029. MKT1.

    Phylogenomic databases

    eggNOGi COG5366.
    HOGENOMi HOG000113598.
    OMAi ARYYQVK.
    OrthoDBi EOG722JJ1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33114-MONOMER.

    Miscellaneous databases

    NextBioi 979863.

    Gene expression databases

    Genevestigatori P40850.

    Family and domain databases

    Gene3Di 3.40.50.1010. 2 hits.
    InterProi IPR022039. MKT1_C.
    IPR022040. MKT1_N.
    IPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    IPR006085. XPG_DNA_repair_N.
    [Graphical view ]
    Pfami PF12246. MKT1_C. 1 hit.
    PF12247. MKT1_N. 1 hit.
    PF00867. XPG_I. 1 hit.
    PF00752. XPG_N. 1 hit.
    [Graphical view ]
    SMARTi SM00484. XPGI. 1 hit.
    SM00485. XPGN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF88723. SSF88723. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of MKT1, needed for propagation of M2 satellite dsRNA of the L-A virus of Saccharomyces cerevisiae."
      Vermut M., Widner W.R., Dinman J.D., Wickner R.B.
      Yeast 10:1477-1479(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 2574.
    2. "Dissecting the architecture of a quantitative trait locus in yeast."
      Steinmetz L.M., Sinha H., Richards D.R., Spiegelman J.I., Oefner P.J., McCusker J.H., Davis R.W.
      Nature 416:326-330(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 200060 / W303, S96, YJM 1129, YJM 270, YJM 627 and YJM 789.
    3. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
      Deutschbauer A.M., Davis R.W.
      Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c, ATCC 204626 / S288c / A364A, ATCC 24657 / D273-10B and Sigma 1278B.
    4. "The sequence of a 17,933 bp segment of Saccharomyces cerevisiae chromosome XIV contains the RHO2, TOP2, MKT1 and END3 genes and five new open reading frames."
      Soler-Mira A., Saiz J.E., Ballesta J.P.G., Remacha M.A.
      Yeast 12:485-491(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-362 AND SER-371, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMKT1_YEAST
    AccessioniPrimary (citable) accession number: P40850
    Secondary accession number(s): D6W194, Q45T81, Q8TF89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3430 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3