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Protein

Protein MKT1

Gene

MKT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for propagation of M2 dsRNA satellite at temperature above 30 degrees Celsius if an L-A RNA virus carrying NEX (N) is present in the cells.

GO - Molecular functioni

  • endodeoxyribonuclease activity Source: SGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • DNA repair Source: InterPro
  • interspecies interaction between organisms Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33114-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MKT1
Gene namesi
Name:MKT1
Ordered Locus Names:YNL085W
ORF Names:N2302
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL085W.
SGDiS000005029. MKT1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: SGD
  • cytoplasmic stress granule Source: SGD
  • nuclear periphery Source: SGD
  • polysome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000964951 – 830Protein MKT1Add BLAST830

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki4Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei358PhosphoserineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Modified residuei371PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40850.
PRIDEiP40850.

PTM databases

iPTMnetiP40850.

Interactioni

Protein-protein interaction databases

BioGridi35738. 74 interactors.
DIPiDIP-6530N.
IntActiP40850. 16 interactors.
MINTiMINT-642141.

Structurei

3D structure databases

ProteinModelPortaliP40850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000113598.
InParanoidiP40850.
OMAiFNIYLRE.
OrthoDBiEOG092C0WW7.

Family and domain databases

Gene3Di3.40.50.1010. 2 hits.
InterProiIPR022039. MKT1_C.
IPR022040. MKT1_N.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 3 hits.
PfamiPF12246. MKT1_C. 1 hit.
PF12247. MKT1_N. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
SMARTiSM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF88723. SSF88723. 2 hits.

Sequencei

Sequence statusi: Complete.

P40850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKSLESFL FERGLVGSYA IEALNNCTLD IDVNHYVSRL LTNKREQYLD
60 70 80 90 100
AIGGFPTSLK MYLESDLKIF KDFNITPIFV FNGGLTYNQL EASGHFTAAS
110 120 130 140 150
ASASISSTTT SSSGTNATTR SNTESVLLQR SRGWTQWNNL ISSNQNSYID
160 170 180 190 200
QPIQPQEPFR HNTTIDSKAY QNDLIAYFIE HGYMYQVAPY SSWFQLAYLL
210 220 230 240 250
NSAYIDAIYG PTDCLMLDCV DRFILGMEFP NKEFRFIDRS RVMKDLGCTH
260 270 280 290 300
EEFIDIAMAV GNDLQPTTLP PLQIYPVPQL FDIALEMVLN TGTNFYAYQL
310 320 330 340 350
STTLQNDSKE NIQNYQRGIS ALRYMPVLKD TGKVELFVQE IVVSEEDSEK
360 370 380 390 400
NNKDGKKSNL SSPSSASSSA SPATTVTKNA SEKLTYEKSS TKEVRKPRDI
410 420 430 440 450
PNDVHDFIGQ MLPHEYYFYR SIGLVTGKLF DAIVTGVYPE EPPLGGGSST
460 470 480 490 500
SYKKLVSKSV EIFKNKEINL LTQPINRYYQ IKQIKQVKWY AANEPTTLTN
510 520 530 540 550
RMSPSMFETI NHLIVKTETS DEKEFSISEF ITTINGSSNM AKDFISEKVI
560 570 580 590 600
FPNSVPIESK LNSPFNLLST NFLRLLVLLE FFTFDFKEKL LEPTRWGEVF
610 620 630 640 650
LKLNELNIDS KYHESVIIFL VFLKCDVLKL DEEVQPPAPS ALSQATLRSY
660 670 680 690 700
PEESLYVLLI TRVLTLFQVD QKPSNYHGPI DKKTLIFRDH LSFIKENLNE
710 720 730 740 750
LFEAVLISSL TSGEFNRLSL DNFGWARKIV RYLPFKLDSP NTIMAMMWEF
760 770 780 790 800
FLQKYLHNGN AKNDALSLVA TEFNTYKSTP NLDEQFVESH RFLLEISKVM
810 820 830
QELNAAKLID ENVFKLFTKA VEFTTTALSS
Length:830
Mass (Da):94,495
Last modified:February 1, 1996 - v2
Checksum:iBD976D8387E4F708
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti809 – 830IDENV…TALSS → TMKTCLNYH in AAC49470 (PubMed:7532890).CuratedAdd BLAST22

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti30D → G in strain: 2574, ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789. 1
Natural varianti453K → R in strain: ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09129 Genomic DNA. Translation: AAC49470.1.
AF458969 Genomic DNA. Translation: AAM00519.1.
AF458975 Genomic DNA. Translation: AAM00555.1.
AF458977 Genomic DNA. Translation: AAM00567.1.
AF458978 Genomic DNA. Translation: AAM00573.1.
AF458980 Genomic DNA. Translation: AAM00585.1.
AF458981 Genomic DNA. Translation: AAM00591.1.
DQ116825 Genomic DNA. Translation: AAZ23277.1.
DQ116826 Genomic DNA. Translation: AAZ23278.1.
DQ116827 Genomic DNA. Translation: AAZ23279.1.
DQ116828 Genomic DNA. Translation: AAZ23280.1.
X89016 Genomic DNA. Translation: CAA61425.1.
Z71361 Genomic DNA. Translation: CAA95961.1.
Z71360 Genomic DNA. Translation: CAA95960.1.
BK006947 Genomic DNA. Translation: DAA10460.1.
PIRiS57537.
RefSeqiNP_014314.3. NM_001182923.3.

Genome annotation databases

EnsemblFungiiYNL085W; YNL085W; YNL085W.
GeneIDi855639.
KEGGisce:YNL085W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09129 Genomic DNA. Translation: AAC49470.1.
AF458969 Genomic DNA. Translation: AAM00519.1.
AF458975 Genomic DNA. Translation: AAM00555.1.
AF458977 Genomic DNA. Translation: AAM00567.1.
AF458978 Genomic DNA. Translation: AAM00573.1.
AF458980 Genomic DNA. Translation: AAM00585.1.
AF458981 Genomic DNA. Translation: AAM00591.1.
DQ116825 Genomic DNA. Translation: AAZ23277.1.
DQ116826 Genomic DNA. Translation: AAZ23278.1.
DQ116827 Genomic DNA. Translation: AAZ23279.1.
DQ116828 Genomic DNA. Translation: AAZ23280.1.
X89016 Genomic DNA. Translation: CAA61425.1.
Z71361 Genomic DNA. Translation: CAA95961.1.
Z71360 Genomic DNA. Translation: CAA95960.1.
BK006947 Genomic DNA. Translation: DAA10460.1.
PIRiS57537.
RefSeqiNP_014314.3. NM_001182923.3.

3D structure databases

ProteinModelPortaliP40850.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35738. 74 interactors.
DIPiDIP-6530N.
IntActiP40850. 16 interactors.
MINTiMINT-642141.

PTM databases

iPTMnetiP40850.

Proteomic databases

MaxQBiP40850.
PRIDEiP40850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL085W; YNL085W; YNL085W.
GeneIDi855639.
KEGGisce:YNL085W.

Organism-specific databases

EuPathDBiFungiDB:YNL085W.
SGDiS000005029. MKT1.

Phylogenomic databases

HOGENOMiHOG000113598.
InParanoidiP40850.
OMAiFNIYLRE.
OrthoDBiEOG092C0WW7.

Enzyme and pathway databases

BioCyciYEAST:G3O-33114-MONOMER.

Miscellaneous databases

PROiP40850.

Family and domain databases

Gene3Di3.40.50.1010. 2 hits.
InterProiIPR022039. MKT1_C.
IPR022040. MKT1_N.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 3 hits.
PfamiPF12246. MKT1_C. 1 hit.
PF12247. MKT1_N. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
SMARTiSM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF88723. SSF88723. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMKT1_YEAST
AccessioniPrimary (citable) accession number: P40850
Secondary accession number(s): D6W194, Q45T81, Q8TF89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3430 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.