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P40850 (MKT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein MKT1
Gene names
Name:MKT1
Ordered Locus Names:YNL085W
ORF Names:N2302
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for propagation of M2 dsRNA satellite at temperature above 30 degrees Celsius if an L-A RNA virus carrying NEX (N) is present in the cells.

Miscellaneous

Present with 3430 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 830830Protein MKT1
PRO_0000096495

Amino acid modifications

Modified residue3581Phosphoserine Ref.9
Modified residue3621Phosphoserine Ref.9
Modified residue3711Phosphoserine Ref.9

Natural variations

Natural variant301D → G in strain: 2574, ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.
Natural variant4531K → R in strain: ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.

Experimental info

Sequence conflict809 – 83022IDENV…TALSS → TMKTCLNYH in AAC49470. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40850 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: BD976D8387E4F708

FASTA83094,495
        10         20         30         40         50         60 
MAIKSLESFL FERGLVGSYA IEALNNCTLD IDVNHYVSRL LTNKREQYLD AIGGFPTSLK 

        70         80         90        100        110        120 
MYLESDLKIF KDFNITPIFV FNGGLTYNQL EASGHFTAAS ASASISSTTT SSSGTNATTR 

       130        140        150        160        170        180 
SNTESVLLQR SRGWTQWNNL ISSNQNSYID QPIQPQEPFR HNTTIDSKAY QNDLIAYFIE 

       190        200        210        220        230        240 
HGYMYQVAPY SSWFQLAYLL NSAYIDAIYG PTDCLMLDCV DRFILGMEFP NKEFRFIDRS 

       250        260        270        280        290        300 
RVMKDLGCTH EEFIDIAMAV GNDLQPTTLP PLQIYPVPQL FDIALEMVLN TGTNFYAYQL 

       310        320        330        340        350        360 
STTLQNDSKE NIQNYQRGIS ALRYMPVLKD TGKVELFVQE IVVSEEDSEK NNKDGKKSNL 

       370        380        390        400        410        420 
SSPSSASSSA SPATTVTKNA SEKLTYEKSS TKEVRKPRDI PNDVHDFIGQ MLPHEYYFYR 

       430        440        450        460        470        480 
SIGLVTGKLF DAIVTGVYPE EPPLGGGSST SYKKLVSKSV EIFKNKEINL LTQPINRYYQ 

       490        500        510        520        530        540 
IKQIKQVKWY AANEPTTLTN RMSPSMFETI NHLIVKTETS DEKEFSISEF ITTINGSSNM 

       550        560        570        580        590        600 
AKDFISEKVI FPNSVPIESK LNSPFNLLST NFLRLLVLLE FFTFDFKEKL LEPTRWGEVF 

       610        620        630        640        650        660 
LKLNELNIDS KYHESVIIFL VFLKCDVLKL DEEVQPPAPS ALSQATLRSY PEESLYVLLI 

       670        680        690        700        710        720 
TRVLTLFQVD QKPSNYHGPI DKKTLIFRDH LSFIKENLNE LFEAVLISSL TSGEFNRLSL 

       730        740        750        760        770        780 
DNFGWARKIV RYLPFKLDSP NTIMAMMWEF FLQKYLHNGN AKNDALSLVA TEFNTYKSTP 

       790        800        810        820        830 
NLDEQFVESH RFLLEISKVM QELNAAKLID ENVFKLFTKA VEFTTTALSS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of MKT1, needed for propagation of M2 satellite dsRNA of the L-A virus of Saccharomyces cerevisiae."
Vermut M., Widner W.R., Dinman J.D., Wickner R.B.
Yeast 10:1477-1479(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 2574.
[2]"Dissecting the architecture of a quantitative trait locus in yeast."
Steinmetz L.M., Sinha H., Richards D.R., Spiegelman J.I., Oefner P.J., McCusker J.H., Davis R.W.
Nature 416:326-330(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 200060 / W303, S96, YJM 1129, YJM 270, YJM 627 and YJM 789.
[3]"Quantitative trait loci mapped to single-nucleotide resolution in yeast."
Deutschbauer A.M., Davis R.W.
Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c, ATCC 204626 / S288c / A364A, ATCC 24657 / D273-10B and Sigma 1278B.
[4]"The sequence of a 17,933 bp segment of Saccharomyces cerevisiae chromosome XIV contains the RHO2, TOP2, MKT1 and END3 genes and five new open reading frames."
Soler-Mira A., Saiz J.E., Ballesta J.P.G., Remacha M.A.
Yeast 12:485-491(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-362 AND SER-371, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09129 Genomic DNA. Translation: AAC49470.1.
AF458969 Genomic DNA. Translation: AAM00519.1.
AF458975 Genomic DNA. Translation: AAM00555.1.
AF458977 Genomic DNA. Translation: AAM00567.1.
AF458978 Genomic DNA. Translation: AAM00573.1.
AF458980 Genomic DNA. Translation: AAM00585.1.
AF458981 Genomic DNA. Translation: AAM00591.1.
DQ116825 Genomic DNA. Translation: AAZ23277.1.
DQ116826 Genomic DNA. Translation: AAZ23278.1.
DQ116827 Genomic DNA. Translation: AAZ23279.1.
DQ116828 Genomic DNA. Translation: AAZ23280.1.
X89016 Genomic DNA. Translation: CAA61425.1.
Z71361 Genomic DNA. Translation: CAA95961.1.
Z71360 Genomic DNA. Translation: CAA95960.1.
BK006947 Genomic DNA. Translation: DAA10460.1.
PIRS57537.
RefSeqNP_014314.3. NM_001182923.3.

3D structure databases

ProteinModelPortalP40850.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35738. 73 interactions.
DIPDIP-6530N.
IntActP40850. 14 interactions.
MINTMINT-642141.

Proteomic databases

MaxQBP40850.
PaxDbP40850.
PeptideAtlasP40850.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL085W; YNL085W; YNL085W.
GeneID855639.
KEGGsce:YNL085W.

Organism-specific databases

CYGDYNL085w.
SGDS000005029. MKT1.

Phylogenomic databases

eggNOGCOG5366.
HOGENOMHOG000113598.
OMAARYYQVK.
OrthoDBEOG722JJ1.

Enzyme and pathway databases

BioCycYEAST:G3O-33114-MONOMER.

Gene expression databases

GenevestigatorP40850.

Family and domain databases

Gene3D3.40.50.1010. 2 hits.
InterProIPR022039. MKT1_C.
IPR022040. MKT1_N.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PfamPF12246. MKT1_C. 1 hit.
PF12247. MKT1_N. 1 hit.
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
SMARTSM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMSSF88723. SSF88723. 2 hits.
ProtoNetSearch...

Other

NextBio979863.

Entry information

Entry nameMKT1_YEAST
AccessionPrimary (citable) accession number: P40850
Secondary accession number(s): D6W194, Q45T81, Q8TF89
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD