P40850 (MKT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MKT1

Gene names

Name:	MKT1
Ordered Locus Names:	YNL085W
ORF Names:	N2302

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for propagation of M2 dsRNA satellite at temperature above 30 degrees Celsius if an L-A RNA virus carrying NEX (N) is present in the cells.
Miscellaneous	Present with 3430 molecules/cell in log phase SD medium.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: InterPro interspecies interaction between organisms Inferred from mutant phenotype Ref.1. Source: SGD response to DNA damage stimulus Inferred from mutant phenotype PubMed 18617998. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095PubMed 22842922. Source: SGD polysome Inferred from direct assay PubMed 15082763. Source: SGD
Molecular_function	nuclease activity Inferred from sequence or structural similarity PubMed 15082763. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 830	830	Protein MKT1		PRO_0000096495
Amino acid modifications
	Modified residue	358	1	Phosphoserine Ref.9
	Modified residue	362	1	Phosphoserine Ref.8 Ref.9 Ref.10
	Modified residue	371	1	Phosphoserine Ref.9
Natural variations
	Natural variant	30	1	D → G in strain: 2574, ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.
	Natural variant	453	1	K → R in strain: ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.
Experimental info
	Sequence conflict	809 – 830	22	IDENV…TALSS → TMKTCLNYH in AAC49470. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40850 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: BD976D8387E4F708	FASTA	830	94,495
	10 20 30 40 50 60 MAIKSLESFL FERGLVGSYA IEALNNCTLD IDVNHYVSRL LTNKREQYLD AIGGFPTSLK 70 80 90 100 110 120 MYLESDLKIF KDFNITPIFV FNGGLTYNQL EASGHFTAAS ASASISSTTT SSSGTNATTR 130 140 150 160 170 180 SNTESVLLQR SRGWTQWNNL ISSNQNSYID QPIQPQEPFR HNTTIDSKAY QNDLIAYFIE 190 200 210 220 230 240 HGYMYQVAPY SSWFQLAYLL NSAYIDAIYG PTDCLMLDCV DRFILGMEFP NKEFRFIDRS 250 260 270 280 290 300 RVMKDLGCTH EEFIDIAMAV GNDLQPTTLP PLQIYPVPQL FDIALEMVLN TGTNFYAYQL 310 320 330 340 350 360 STTLQNDSKE NIQNYQRGIS ALRYMPVLKD TGKVELFVQE IVVSEEDSEK NNKDGKKSNL 370 380 390 400 410 420 SSPSSASSSA SPATTVTKNA SEKLTYEKSS TKEVRKPRDI PNDVHDFIGQ MLPHEYYFYR 430 440 450 460 470 480 SIGLVTGKLF DAIVTGVYPE EPPLGGGSST SYKKLVSKSV EIFKNKEINL LTQPINRYYQ 490 500 510 520 530 540 IKQIKQVKWY AANEPTTLTN RMSPSMFETI NHLIVKTETS DEKEFSISEF ITTINGSSNM 550 560 570 580 590 600 AKDFISEKVI FPNSVPIESK LNSPFNLLST NFLRLLVLLE FFTFDFKEKL LEPTRWGEVF 610 620 630 640 650 660 LKLNELNIDS KYHESVIIFL VFLKCDVLKL DEEVQPPAPS ALSQATLRSY PEESLYVLLI 670 680 690 700 710 720 TRVLTLFQVD QKPSNYHGPI DKKTLIFRDH LSFIKENLNE LFEAVLISSL TSGEFNRLSL 730 740 750 760 770 780 DNFGWARKIV RYLPFKLDSP NTIMAMMWEF FLQKYLHNGN AKNDALSLVA TEFNTYKSTP 790 800 810 820 830 NLDEQFVESH RFLLEISKVM QELNAAKLID ENVFKLFTKA VEFTTTALSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of MKT1, needed for propagation of M2 satellite dsRNA of the L-A virus of Saccharomyces cerevisiae." Vermut M., Widner W.R., Dinman J.D., Wickner R.B. Yeast 10:1477-1479(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2574.
[2]	"Dissecting the architecture of a quantitative trait locus in yeast." Steinmetz L.M., Sinha H., Richards D.R., Spiegelman J.I., Oefner P.J., McCusker J.H., Davis R.W. Nature 416:326-330(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200060 / W303, S96, YJM 1129, YJM 270, YJM 627 and YJM 789.
[3]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c, ATCC 204626 / S288c / A364A, ATCC 24657 / D273-10B and Sigma 1278B.
[4]	"The sequence of a 17,933 bp segment of Saccharomyces cerevisiae chromosome XIV contains the RHO2, TOP2, MKT1 and END3 genes and five new open reading frames." Soler-Mira A., Saiz J.E., Ballesta J.P.G., Remacha M.A. Yeast 12:485-491(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-362 AND SER-371, MASS SPECTROMETRY. Strain: ADR376.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09129 Genomic DNA. Translation: AAC49470.1. AF458969 Genomic DNA. Translation: AAM00519.1. AF458975 Genomic DNA. Translation: AAM00555.1. AF458977 Genomic DNA. Translation: AAM00567.1. AF458978 Genomic DNA. Translation: AAM00573.1. AF458980 Genomic DNA. Translation: AAM00585.1. AF458981 Genomic DNA. Translation: AAM00591.1. DQ116825 Genomic DNA. Translation: AAZ23277.1. DQ116826 Genomic DNA. Translation: AAZ23278.1. DQ116827 Genomic DNA. Translation: AAZ23279.1. DQ116828 Genomic DNA. Translation: AAZ23280.1. X89016 Genomic DNA. Translation: CAA61425.1. Z71361 Genomic DNA. Translation: CAA95961.1. Z71360 Genomic DNA. Translation: CAA95960.1. BK006947 Genomic DNA. Translation: DAA10460.1.
PIR	S57537.
RefSeq	NP_014314.3. NM_001182923.3.
3D structure databases
ProteinModelPortal	P40850.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6530N.
IntAct	P40850. 25 interactions.
MINT	MINT-642141.
Proteomic databases
PaxDb	P40850.
PeptideAtlas	P40850.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL085W; YNL085W; YNL085W.
GeneID	855639.
KEGG	sce:YNL078W. sce:YNL085W.
Organism-specific databases
CYGD	YNL085w.
SGD	S000005029. MKT1.
Phylogenomic databases
eggNOG	COG5366.
HOGENOM	HOG000113598.
OMA	ESYLFER.
OrthoDB	EOG43XZBR.
Gene expression databases
ArrayExpress	P40850.
Genevestigator	P40850.
GermOnline	YNL085W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022039. MKT1_C. IPR022040. MKT1_N. IPR006086. XPG-I_dom. IPR006085. XPG_DNA_repair_N. [Graphical view]
Pfam	PF12246. MKT1_C. 1 hit. PF12247. MKT1_N. 1 hit. PF00867. XPG_I. 1 hit. PF00752. XPG_N. 1 hit. [Graphical view]
SMART	SM00484. XPGI. 1 hit. SM00485. XPGN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979863.

Entry information

Entry name

MKT1_YEAST

Accession

Primary (citable) accession number: P40850
Secondary accession number(s): D6W194, Q45T81, Q8TF89

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program