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P40850

- MKT1_YEAST

UniProt

P40850 - MKT1_YEAST

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Protein
Protein MKT1
Gene
MKT1, YNL085W, N2302
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Required for propagation of M2 dsRNA satellite at temperature above 30 degrees Celsius if an L-A RNA virus carrying NEX (N) is present in the cells.

GO - Molecular functioni

  1. nuclease activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. DNA repair Source: InterPro
  2. cellular response to DNA damage stimulus Source: SGD
  3. interspecies interaction between organisms Source: SGD
  4. nucleic acid phosphodiester bond hydrolysis Source: GOC
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33114-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MKT1
Gene namesi
Name:MKT1
Ordered Locus Names:YNL085W
ORF Names:N2302
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL085w.
SGDiS000005029. MKT1.

Subcellular locationi

GO - Cellular componenti

  1. polysome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Protein MKT1
PRO_0000096495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei358 – 3581Phosphoserine1 Publication
Modified residuei362 – 3621Phosphoserine1 Publication
Modified residuei371 – 3711Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40850.
PaxDbiP40850.
PeptideAtlasiP40850.

Expressioni

Gene expression databases

GenevestigatoriP40850.

Interactioni

Protein-protein interaction databases

BioGridi35738. 73 interactions.
DIPiDIP-6530N.
IntActiP40850. 14 interactions.
MINTiMINT-642141.

Structurei

3D structure databases

ProteinModelPortaliP40850.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG5366.
HOGENOMiHOG000113598.
OMAiARYYQVK.
OrthoDBiEOG722JJ1.

Family and domain databases

Gene3Di3.40.50.1010. 2 hits.
InterProiIPR022039. MKT1_C.
IPR022040. MKT1_N.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PfamiPF12246. MKT1_C. 1 hit.
PF12247. MKT1_N. 1 hit.
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
SMARTiSM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF88723. SSF88723. 2 hits.

Sequencei

Sequence statusi: Complete.

P40850-1 [UniParc]FASTAAdd to Basket

« Hide

MAIKSLESFL FERGLVGSYA IEALNNCTLD IDVNHYVSRL LTNKREQYLD    50
AIGGFPTSLK MYLESDLKIF KDFNITPIFV FNGGLTYNQL EASGHFTAAS 100
ASASISSTTT SSSGTNATTR SNTESVLLQR SRGWTQWNNL ISSNQNSYID 150
QPIQPQEPFR HNTTIDSKAY QNDLIAYFIE HGYMYQVAPY SSWFQLAYLL 200
NSAYIDAIYG PTDCLMLDCV DRFILGMEFP NKEFRFIDRS RVMKDLGCTH 250
EEFIDIAMAV GNDLQPTTLP PLQIYPVPQL FDIALEMVLN TGTNFYAYQL 300
STTLQNDSKE NIQNYQRGIS ALRYMPVLKD TGKVELFVQE IVVSEEDSEK 350
NNKDGKKSNL SSPSSASSSA SPATTVTKNA SEKLTYEKSS TKEVRKPRDI 400
PNDVHDFIGQ MLPHEYYFYR SIGLVTGKLF DAIVTGVYPE EPPLGGGSST 450
SYKKLVSKSV EIFKNKEINL LTQPINRYYQ IKQIKQVKWY AANEPTTLTN 500
RMSPSMFETI NHLIVKTETS DEKEFSISEF ITTINGSSNM AKDFISEKVI 550
FPNSVPIESK LNSPFNLLST NFLRLLVLLE FFTFDFKEKL LEPTRWGEVF 600
LKLNELNIDS KYHESVIIFL VFLKCDVLKL DEEVQPPAPS ALSQATLRSY 650
PEESLYVLLI TRVLTLFQVD QKPSNYHGPI DKKTLIFRDH LSFIKENLNE 700
LFEAVLISSL TSGEFNRLSL DNFGWARKIV RYLPFKLDSP NTIMAMMWEF 750
FLQKYLHNGN AKNDALSLVA TEFNTYKSTP NLDEQFVESH RFLLEISKVM 800
QELNAAKLID ENVFKLFTKA VEFTTTALSS 830
Length:830
Mass (Da):94,495
Last modified:February 1, 1996 - v2
Checksum:iBD976D8387E4F708
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301D → G in strain: 2574, ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.
Natural varianti453 – 4531K → R in strain: ATCC 24657 / D273-10B, ATCC 200060 / W303, Sigma 1278B, YJM 1129, YJM 270, YJM 627 and YJM 789.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti809 – 83022IDENV…TALSS → TMKTCLNYH in AAC49470. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09129 Genomic DNA. Translation: AAC49470.1.
AF458969 Genomic DNA. Translation: AAM00519.1.
AF458975 Genomic DNA. Translation: AAM00555.1.
AF458977 Genomic DNA. Translation: AAM00567.1.
AF458978 Genomic DNA. Translation: AAM00573.1.
AF458980 Genomic DNA. Translation: AAM00585.1.
AF458981 Genomic DNA. Translation: AAM00591.1.
DQ116825 Genomic DNA. Translation: AAZ23277.1.
DQ116826 Genomic DNA. Translation: AAZ23278.1.
DQ116827 Genomic DNA. Translation: AAZ23279.1.
DQ116828 Genomic DNA. Translation: AAZ23280.1.
X89016 Genomic DNA. Translation: CAA61425.1.
Z71361 Genomic DNA. Translation: CAA95961.1.
Z71360 Genomic DNA. Translation: CAA95960.1.
BK006947 Genomic DNA. Translation: DAA10460.1.
PIRiS57537.
RefSeqiNP_014314.3. NM_001182923.3.

Genome annotation databases

EnsemblFungiiYNL085W; YNL085W; YNL085W.
GeneIDi855639.
KEGGisce:YNL085W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09129 Genomic DNA. Translation: AAC49470.1 .
AF458969 Genomic DNA. Translation: AAM00519.1 .
AF458975 Genomic DNA. Translation: AAM00555.1 .
AF458977 Genomic DNA. Translation: AAM00567.1 .
AF458978 Genomic DNA. Translation: AAM00573.1 .
AF458980 Genomic DNA. Translation: AAM00585.1 .
AF458981 Genomic DNA. Translation: AAM00591.1 .
DQ116825 Genomic DNA. Translation: AAZ23277.1 .
DQ116826 Genomic DNA. Translation: AAZ23278.1 .
DQ116827 Genomic DNA. Translation: AAZ23279.1 .
DQ116828 Genomic DNA. Translation: AAZ23280.1 .
X89016 Genomic DNA. Translation: CAA61425.1 .
Z71361 Genomic DNA. Translation: CAA95961.1 .
Z71360 Genomic DNA. Translation: CAA95960.1 .
BK006947 Genomic DNA. Translation: DAA10460.1 .
PIRi S57537.
RefSeqi NP_014314.3. NM_001182923.3.

3D structure databases

ProteinModelPortali P40850.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35738. 73 interactions.
DIPi DIP-6530N.
IntActi P40850. 14 interactions.
MINTi MINT-642141.

Proteomic databases

MaxQBi P40850.
PaxDbi P40850.
PeptideAtlasi P40850.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL085W ; YNL085W ; YNL085W .
GeneIDi 855639.
KEGGi sce:YNL085W.

Organism-specific databases

CYGDi YNL085w.
SGDi S000005029. MKT1.

Phylogenomic databases

eggNOGi COG5366.
HOGENOMi HOG000113598.
OMAi ARYYQVK.
OrthoDBi EOG722JJ1.

Enzyme and pathway databases

BioCyci YEAST:G3O-33114-MONOMER.

Miscellaneous databases

NextBioi 979863.

Gene expression databases

Genevestigatori P40850.

Family and domain databases

Gene3Di 3.40.50.1010. 2 hits.
InterProi IPR022039. MKT1_C.
IPR022040. MKT1_N.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006085. XPG_DNA_repair_N.
[Graphical view ]
Pfami PF12246. MKT1_C. 1 hit.
PF12247. MKT1_N. 1 hit.
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view ]
SMARTi SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view ]
SUPFAMi SSF88723. SSF88723. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of MKT1, needed for propagation of M2 satellite dsRNA of the L-A virus of Saccharomyces cerevisiae."
    Vermut M., Widner W.R., Dinman J.D., Wickner R.B.
    Yeast 10:1477-1479(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 2574.
  2. "Dissecting the architecture of a quantitative trait locus in yeast."
    Steinmetz L.M., Sinha H., Richards D.R., Spiegelman J.I., Oefner P.J., McCusker J.H., Davis R.W.
    Nature 416:326-330(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 200060 / W303, S96, YJM 1129, YJM 270, YJM 627 and YJM 789.
  3. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c, ATCC 204626 / S288c / A364A, ATCC 24657 / D273-10B and Sigma 1278B.
  4. "The sequence of a 17,933 bp segment of Saccharomyces cerevisiae chromosome XIV contains the RHO2, TOP2, MKT1 and END3 genes and five new open reading frames."
    Soler-Mira A., Saiz J.E., Ballesta J.P.G., Remacha M.A.
    Yeast 12:485-491(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-362 AND SER-371, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMKT1_YEAST
AccessioniPrimary (citable) accession number: P40850
Secondary accession number(s): D6W194, Q45T81, Q8TF89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3430 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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