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Protein

5'-3' exoribonuclease 2

Gene

dhp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the processing of nuclear mRNA and rRNA precursors. May promote the termination of transcription by RNA polymerase II (By similarity). Essential for vegetative cell growth and chromosome segregation. Possesses 5'->3' exoribonuclease activity.By similarity

Catalytic activityi

Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates.

GO - Molecular functioni

  • 5'-3' exoribonuclease activity Source: PomBase
  • nucleic acid binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, Transcription, Transcription regulation, Transcription termination

Names & Taxonomyi

Protein namesi
Recommended name:
5'-3' exoribonuclease 2 (EC:3.1.13.-)
Alternative name(s):
Protein dhp1
Gene namesi
Name:dhp1
ORF Names:SPAC26A3.12c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC26A3.12c.
PomBaseiSPAC26A3.12c. dhp1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9919915'-3' exoribonuclease 2PRO_0000071398Add
BLAST

Proteomic databases

MaxQBiP40848.

Interactioni

Protein-protein interaction databases

BioGridi279127. 18 interactions.
DIPiDIP-59743N.
MINTiMINT-4689803.

Structurei

Secondary structure

1
991
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1311Combined sources
Helixi15 – 173Combined sources
Beta strandi18 – 203Combined sources
Beta strandi49 – 557Combined sources
Helixi57 – 648Combined sources
Beta strandi67 – 693Combined sources
Helixi75 – 9319Combined sources
Beta strandi95 – 1028Combined sources
Helixi109 – 14335Combined sources
Helixi149 – 1524Combined sources
Helixi159 – 1613Combined sources
Helixi167 – 18418Combined sources
Helixi188 – 1903Combined sources
Beta strandi194 – 1985Combined sources
Helixi206 – 21813Combined sources
Beta strandi229 – 2324Combined sources
Helixi238 – 2447Combined sources
Beta strandi248 – 2558Combined sources
Turni269 – 2735Combined sources
Beta strandi292 – 2965Combined sources
Helixi297 – 30812Combined sources
Helixi319 – 32911Combined sources
Helixi330 – 3334Combined sources
Beta strandi336 – 3383Combined sources
Helixi346 – 3483Combined sources
Helixi350 – 36516Combined sources
Beta strandi369 – 3713Combined sources
Helixi377 – 38812Combined sources
Helixi391 – 40212Combined sources
Turni403 – 4053Combined sources
Helixi591 – 5988Combined sources
Helixi608 – 62922Combined sources
Helixi647 – 6493Combined sources
Helixi668 – 6758Combined sources
Helixi678 – 6836Combined sources
Helixi686 – 6938Combined sources
Helixi700 – 7023Combined sources
Beta strandi712 – 7154Combined sources
Beta strandi719 – 7224Combined sources
Helixi729 – 7379Combined sources
Helixi740 – 7423Combined sources
Helixi745 – 7495Combined sources
Beta strandi757 – 7615Combined sources
Helixi767 – 7748Combined sources
Beta strandi776 – 7783Combined sources
Beta strandi780 – 7823Combined sources
Helixi788 – 7914Combined sources
Beta strandi796 – 8005Combined sources
Helixi819 – 8246Combined sources
Beta strandi828 – 8303Combined sources
Beta strandi833 – 8397Combined sources
Helixi864 – 87310Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FQDX-ray2.20A1-885[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi264 – 2685Nuclear localization signalSequence analysis

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000205514.
InParanoidiP40848.
KOiK12619.
OMAiGSEITHQ.
OrthoDBiEOG71RXT2.
PhylomeDBiP40848.

Family and domain databases

InterProiIPR027073. 5_3_exoribonuclease.
IPR017151. 5_3_exoribonuclease_2/3.
IPR004859. Put_53exo.
[Graphical view]
PANTHERiPTHR12341. PTHR12341. 2 hits.
PfamiPF03159. XRN_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037239. Exonuclease_Xrn2. 1 hit.

Sequencei

Sequence statusi: Complete.

P40848-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVPALFRLL SRKFAKVITP VIEAPTEKLP DGTEIEPDLS LPNPNGVECD
60 70 80 90 100
NLYLDMNGIV HPCSHPEDRP APETEDEMMV AVFEYTDRIL AMVRPRQLLF
110 120 130 140 150
IAIDGVAPRA KMNQQRSRRF RSSREAALKE EELQAFIEEA KQQGIPIDEN
160 170 180 190 200
ATKKKSWDSN CITPGTPFMD TLAKSLRYYI INKLNSDPCW RNVRFILSDA
210 220 230 240 250
SVPGEGEHKI MEFIRSQRVK PEYDPNTHHV VYGLDADLIM LGLATHEPHF
260 270 280 290 300
RVLREDVFFQ QGSTKKTKEE RLGIKRLDDV SETNKVPVKK PFIWLNVSIL
310 320 330 340 350
REYLEVELYV PNLPFPFDLE RAIDDWVFFI FFVGNDFLPH LPSLDIRDGA
360 370 380 390 400
VERLTEIWRA SLPHMGGYLT LDGSVNLARA EVILSAVGNQ EDDIFKRLKQ
410 420 430 440 450
QEDRRNENYR RRQQRESNQE SESYVDNVVI QRSVETQSTE VVTSSKSTSV
460 470 480 490 500
DTKPPKKTQK IDAPAPVDLV NLSEKTSNRS LGATNRELIN NRAANRLGLS
510 520 530 540 550
REAAAVSSVN KLAASALKAQ LVSNETLQNV PLEDSIASSS AYEDTDSIES
560 570 580 590 600
STPVVHPIDT KVSNVGQKRK APDSTEENEN TDTVRLYEPG YRERYYEQKF
610 620 630 640 650
HISPDEPEKI REAVKHYVHG LCWVLLYYYQ GCPSWTWYYP YHYAPFAADF
660 670 680 690 700
KDLASIDVKF ELNQPFKPYE QLLGVLPAAS KNNLPEKLQT LMTDENSEII
710 720 730 740 750
DFYPENFTID LNGKKFEWQG VALLPFIDEN RLLNAVSKIY PQLTEEESKR
760 770 780 790 800
NEDGSTLLFI SEHHPMFSEL VKQLYSKKRQ GKPLKLSGKM AHGLFGKVNT
810 820 830 840 850
NDSVIPNVSV QCPIDVTSAD ALQKYGSIDD NQSISLVFEV PKSHFVHKSM
860 870 880 890 900
LLRGVKMPNR VLTPEDINQV RAERSFSSRR NNGNSYRGGH QSYGVRRSYQ
910 920 930 940 950
SQSYSSRQSY TGVTNGFANG GVQPPWSGNG NFPRSNASYN SRGGHEGYGG
960 970 980 990
RSRGGGYSNG PPAGNHYSSN RGKGYGYQRE SYNNNNRNGY Y
Length:991
Mass (Da):112,368
Last modified:February 1, 1995 - v1
Checksum:i83FA34D93DADAD00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17752 Genomic DNA. Translation: BAA04601.1.
CU329670 Genomic DNA. Translation: CAA93235.1.
PIRiS43891.
RefSeqiNP_594155.1. NM_001019579.2.

Genome annotation databases

EnsemblFungiiSPAC26A3.12c.1; SPAC26A3.12c.1:pep; SPAC26A3.12c.
GeneIDi2542674.
KEGGispo:SPAC26A3.12c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17752 Genomic DNA. Translation: BAA04601.1.
CU329670 Genomic DNA. Translation: CAA93235.1.
PIRiS43891.
RefSeqiNP_594155.1. NM_001019579.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FQDX-ray2.20A1-885[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279127. 18 interactions.
DIPiDIP-59743N.
MINTiMINT-4689803.

Proteomic databases

MaxQBiP40848.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC26A3.12c.1; SPAC26A3.12c.1:pep; SPAC26A3.12c.
GeneIDi2542674.
KEGGispo:SPAC26A3.12c.

Organism-specific databases

EuPathDBiFungiDB:SPAC26A3.12c.
PomBaseiSPAC26A3.12c. dhp1.

Phylogenomic databases

HOGENOMiHOG000205514.
InParanoidiP40848.
KOiK12619.
OMAiGSEITHQ.
OrthoDBiEOG71RXT2.
PhylomeDBiP40848.

Miscellaneous databases

NextBioi20803722.
PROiP40848.

Family and domain databases

InterProiIPR027073. 5_3_exoribonuclease.
IPR017151. 5_3_exoribonuclease_2/3.
IPR004859. Put_53exo.
[Graphical view]
PANTHERiPTHR12341. PTHR12341. 2 hits.
PfamiPF03159. XRN_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037239. Exonuclease_Xrn2. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of the dhp1+ gene of Schizosaccharomyces pombe: an essential gene that has homology to the DST2 and RAT1 genes of Saccharomyces cerevisiae."
    Sugano S., Shobuike T., Takeda T., Sugino A., Ikeda H.
    Mol. Gen. Genet. 243:1-8(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-29.
    Strain: ATCC 38365 / 975.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The dhp1+ gene, encoding a putative nuclear 5'3' exoribonuclease, is required for proper chromosome segregation in fission yeast."
    Shobuike T., Tatebayashi K., Tani T., Sugano S., Ikeda H.
    Nucleic Acids Res. 29:1326-1333(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiXRN2_SCHPO
AccessioniPrimary (citable) accession number: P40848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 13, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.