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Protein

Holliday junction ATP-dependent DNA helicase RuvA

Gene

ruvA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvAUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvAUniRule annotation
Ordered Locus Names:ML0482
ORF Names:B1177_C2_188
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0482.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203Holliday junction ATP-dependent DNA helicase RuvAPRO_0000094649Add
BLAST

Interactioni

Subunit structurei

Forms a complex with RuvB.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0482.

Structurei

Secondary structure

1
203
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54Combined sources
Beta strandi7 – 126Combined sources
Beta strandi17 – 215Combined sources
Beta strandi24 – 263Combined sources
Helixi32 – 354Combined sources
Beta strandi48 – 536Combined sources
Beta strandi56 – 616Combined sources
Helixi65 – 7511Combined sources
Beta strandi77 – 793Combined sources
Helixi82 – 9110Combined sources
Helixi94 – 1007Combined sources
Turni101 – 1044Combined sources
Helixi106 – 1105Combined sources
Helixi117 – 12610Combined sources
Helixi129 – 1313Combined sources
Helixi150 – 1534Combined sources
Helixi156 – 1616Combined sources
Helixi165 – 17713Combined sources
Helixi188 – 1936Combined sources
Turni197 – 1993Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BVSX-ray3.00A/B/C/D/E/F/G/H1-203[»]
ProteinModelPortaliP40832.
SMRiP40832. Positions 1-202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40832.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KA4. Bacteria.
COG0632. LUCA.
HOGENOMiHOG000057115.
KOiK03550.
OMAiRNQETIF.
OrthoDBiPOG091H02EF.

Family and domain databases

CDDicd14332. UBA_RuvA_C. 1 hit.
Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA. 1 hit.
InterProiIPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
IPR011114. RuvA_C.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.

Sequencei

Sequence statusi: Complete.

P40832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFSVRGEVL EVALDHAVIE AAGIGYRVNA TPSALATLRQ GSQARLVTAM
60 70 80 90 100
VVREDSMTLY GFSDAENRDL FLALLSVSGV GPRLAMATLA VHDAAALRQA
110 120 130 140 150
LADSDVASLT RVPGIGKRGA ERIVLELRDK VGPVGASGLT VGTAADGNAV
160 170 180 190 200
RGSVVEALVG LGFAAKQAEE ATDQVLDGEL GKDGAVATSS ALRAALSLLG

KTR
Length:203
Mass (Da):20,751
Last modified:February 1, 1995 - v1
Checksum:i2C76B0144DB95006
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00011 Genomic DNA. Translation: AAA17095.1.
AL583918 Genomic DNA. Translation: CAC29990.1.
PIRiS72731.
RefSeqiNP_301422.1. NC_002677.1.
WP_010907746.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29990; CAC29990; CAC29990.
GeneIDi909231.
KEGGimle:ML0482.
PATRICi18051796. VBIMycLep78757_0845.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00011 Genomic DNA. Translation: AAA17095.1.
AL583918 Genomic DNA. Translation: CAC29990.1.
PIRiS72731.
RefSeqiNP_301422.1. NC_002677.1.
WP_010907746.1. NC_002677.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BVSX-ray3.00A/B/C/D/E/F/G/H1-203[»]
ProteinModelPortaliP40832.
SMRiP40832. Positions 1-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC29990; CAC29990; CAC29990.
GeneIDi909231.
KEGGimle:ML0482.
PATRICi18051796. VBIMycLep78757_0845.

Organism-specific databases

LepromaiML0482.

Phylogenomic databases

eggNOGiENOG4105KA4. Bacteria.
COG0632. LUCA.
HOGENOMiHOG000057115.
KOiK03550.
OMAiRNQETIF.
OrthoDBiPOG091H02EF.

Miscellaneous databases

EvolutionaryTraceiP40832.

Family and domain databases

CDDicd14332. UBA_RuvA_C. 1 hit.
Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA. 1 hit.
InterProiIPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
IPR011114. RuvA_C.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVA_MYCLE
AccessioniPrimary (citable) accession number: P40832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.