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P40826

- UBP14_RABIT

UniProt

P40826 - UBP14_RABIT

Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

USP14

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs) By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141NucleophilePROSITE-ProRule annotation
    Active sitei434 – 4341Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. endopeptidase inhibitor activity Source: Ensembl
    3. ubiquitin thiolesterase activity Source: Ensembl

    GO - Biological processi

    1. regulation of chemotaxis Source: Ensembl
    2. regulation of proteasomal protein catabolic process Source: Ensembl
    3. synaptic transmission Source: Ensembl
    4. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.015.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 14
    Ubiquitin thioesterase 14
    Ubiquitin-specific-processing protease 14
    Gene namesi
    Name:USP14
    Synonyms:TGT
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Chromosome 9

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. cytoplasmic membrane-bounded vesicle Source: Ensembl
    3. plasma membrane Source: UniProtKB-SubCell
    4. proteasome complex Source: UniProtKB-KW
    5. synapse Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493Ubiquitin carboxyl-terminal hydrolase 14PRO_0000080639Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei143 – 1431PhosphoserineBy similarity
    Modified residuei448 – 4481N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP40826.

    Interactioni

    Subunit structurei

    Homodimer Potential. Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1 By similarity.By similarityCurated

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000015687.

    Structurei

    3D structure databases

    ProteinModelPortaliP40826.
    SMRiP40826. Positions 6-86, 98-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 8077Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini105 – 482378USPAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiNOG286607.
    GeneTreeiENSGT00390000009615.
    HOGENOMiHOG000202292.
    HOVERGENiHBG054185.
    OMAiDWGNLKI.
    TreeFamiTF314494.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40826-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLYSVTVKW GKEKFGGVEL NTDEPPMVFK AQLFALTGVQ PARQRVMVKG    50
    GTLKDDDWGN IKIKNGMTIL MMGSADALPE EPSAKTVFVE DMTEEQLASA 100
    MELPCGLTNL GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ 150
    YITAALRDLF DSMDKTSSSI PPIILLQFLH MAFPQFAEKG EQGQYLQQDA 200
    NECWIQMMRV LQQKLEAIED DSVKETDSSA AAVAPSKKKS LIDQFFGVEF 250
    ETTMKCTESE EEEVTKGKEN QLQLSCFINQ EVKYLFTGLK LRLQEEITKQ 300
    SPTLQRNALY IKSSKISRLP AYLTIQMVRF FYKEKESVNA KVLKDVKFPL 350
    MLDVYELCTP ELQEKMVSFR SKFKDIEDKK VNQQPNASDK KSSPQKEVRY 400
    EPFSFADDIG SNNCGYYDLQ AVLTHQGRSS SSGHYVSWVK RKHDEWIKFD 450
    DDKVSIVTPE DILRLSGGGD WHIAYVLLYG PRRVEIMEEE NEQ 493
    Length:493
    Mass (Da):55,922
    Last modified:January 23, 2007 - v3
    Checksum:i598553BF435E6495
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L37420 mRNA. Translation: AAA96133.1.
    PIRiS68430.
    RefSeqiNP_001075726.1. NM_001082257.1.
    UniGeneiOcu.2060.

    Genome annotation databases

    EnsembliENSOCUT00000029186; ENSOCUP00000015687; ENSOCUG00000011595.
    GeneIDi100009078.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L37420 mRNA. Translation: AAA96133.1 .
    PIRi S68430.
    RefSeqi NP_001075726.1. NM_001082257.1.
    UniGenei Ocu.2060.

    3D structure databases

    ProteinModelPortali P40826.
    SMRi P40826. Positions 6-86, 98-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000015687.

    Protein family/group databases

    MEROPSi C19.015.

    Proteomic databases

    PRIDEi P40826.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSOCUT00000029186 ; ENSOCUP00000015687 ; ENSOCUG00000011595 .
    GeneIDi 100009078.

    Organism-specific databases

    CTDi 9097.

    Phylogenomic databases

    eggNOGi NOG286607.
    GeneTreei ENSGT00390000009615.
    HOGENOMi HOG000202292.
    HOVERGENi HBG054185.
    OMAi DWGNLKI.
    TreeFami TF314494.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit."
      Deshpande K.L., Seubert P.H., Tillman D.M., Farkas W.R., Katze J.R.
      Arch. Biochem. Biophys. 326:1-7(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: New Zealand white.
      Tissue: Liver.

    Entry informationi

    Entry nameiUBP14_RABIT
    AccessioniPrimary (citable) accession number: P40826
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a guanine tRNA-ribosyltransferase.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3