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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

USP14

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114NucleophilePROSITE-ProRule annotation1
Active sitei434Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:USP14
Synonyms:TGT
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Chromosome 9

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806391 – 493Ubiquitin carboxyl-terminal hydrolase 14Add BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphothreonineBy similarity1
Modified residuei143PhosphoserineBy similarity1
Modified residuei148PhosphoserineBy similarity1
Modified residuei236PhosphoserineBy similarity1
Modified residuei301PhosphoserineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei448N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP40826.

Interactioni

Subunit structurei

Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1 (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000015687.

Structurei

3D structure databases

ProteinModelPortaliP40826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 80Ubiquitin-likePROSITE-ProRule annotationAdd BLAST77
Domaini105 – 482USPAdd BLAST378

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1872. Eukaryota.
ENOG410XP96. LUCA.
GeneTreeiENSGT00390000009615.
HOGENOMiHOG000202292.
HOVERGENiHBG054185.
InParanoidiP40826.
KOiK11843.
OMAiFKVKVKW.
OrthoDBiEOG091G06MR.
TreeFamiTF314494.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40826-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLYSVTVKW GKEKFGGVEL NTDEPPMVFK AQLFALTGVQ PARQRVMVKG
60 70 80 90 100
GTLKDDDWGN IKIKNGMTIL MMGSADALPE EPSAKTVFVE DMTEEQLASA
110 120 130 140 150
MELPCGLTNL GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ
160 170 180 190 200
YITAALRDLF DSMDKTSSSI PPIILLQFLH MAFPQFAEKG EQGQYLQQDA
210 220 230 240 250
NECWIQMMRV LQQKLEAIED DSVKETDSSA AAVAPSKKKS LIDQFFGVEF
260 270 280 290 300
ETTMKCTESE EEEVTKGKEN QLQLSCFINQ EVKYLFTGLK LRLQEEITKQ
310 320 330 340 350
SPTLQRNALY IKSSKISRLP AYLTIQMVRF FYKEKESVNA KVLKDVKFPL
360 370 380 390 400
MLDVYELCTP ELQEKMVSFR SKFKDIEDKK VNQQPNASDK KSSPQKEVRY
410 420 430 440 450
EPFSFADDIG SNNCGYYDLQ AVLTHQGRSS SSGHYVSWVK RKHDEWIKFD
460 470 480 490
DDKVSIVTPE DILRLSGGGD WHIAYVLLYG PRRVEIMEEE NEQ
Length:493
Mass (Da):55,922
Last modified:January 23, 2007 - v3
Checksum:i598553BF435E6495
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37420 mRNA. Translation: AAA96133.1.
PIRiS68430.
RefSeqiNP_001075726.1. NM_001082257.1.
UniGeneiOcu.2060.

Genome annotation databases

EnsembliENSOCUT00000029186; ENSOCUP00000015687; ENSOCUG00000011595.
GeneIDi100009078.
KEGGiocu:100009078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37420 mRNA. Translation: AAA96133.1.
PIRiS68430.
RefSeqiNP_001075726.1. NM_001082257.1.
UniGeneiOcu.2060.

3D structure databases

ProteinModelPortaliP40826.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000015687.

Protein family/group databases

MEROPSiC19.015.

Proteomic databases

PRIDEiP40826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSOCUT00000029186; ENSOCUP00000015687; ENSOCUG00000011595.
GeneIDi100009078.
KEGGiocu:100009078.

Organism-specific databases

CTDi9097.

Phylogenomic databases

eggNOGiKOG1872. Eukaryota.
ENOG410XP96. LUCA.
GeneTreeiENSGT00390000009615.
HOGENOMiHOG000202292.
HOVERGENiHBG054185.
InParanoidiP40826.
KOiK11843.
OMAiFKVKVKW.
OrthoDBiEOG091G06MR.
TreeFamiTF314494.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP14_RABIT
AccessioniPrimary (citable) accession number: P40826
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a guanine tRNA-ribosyltransferase.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.