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P40826 (UBP14_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 14

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene names
Name:USP14
Synonyms:TGT
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs) By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Homodimer Potential. Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1 By similarity.

Subcellular location

Cytoplasm By similarity. Cell membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

Contains 1 ubiquitin-like domain.

Contains 1 USP domain.

Caution

Was originally (Ref.1) thought to be a guanine tRNA-ribosyltransferase.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Ubiquitin carboxyl-terminal hydrolase 14
PRO_0000080639

Regions

Domain4 – 8077Ubiquitin-like
Domain105 – 482378USP

Sites

Active site1141Nucleophile By similarity
Active site4341Proton acceptor By similarity

Amino acid modifications

Modified residue1431Phosphoserine By similarity
Modified residue4481N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P40826 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 598553BF435E6495

FASTA49355,922
        10         20         30         40         50         60 
MPLYSVTVKW GKEKFGGVEL NTDEPPMVFK AQLFALTGVQ PARQRVMVKG GTLKDDDWGN 

        70         80         90        100        110        120 
IKIKNGMTIL MMGSADALPE EPSAKTVFVE DMTEEQLASA MELPCGLTNL GNTCYMNATV 

       130        140        150        160        170        180 
QCIRSVPELK DALKRYAGAL RASGEMASAQ YITAALRDLF DSMDKTSSSI PPIILLQFLH 

       190        200        210        220        230        240 
MAFPQFAEKG EQGQYLQQDA NECWIQMMRV LQQKLEAIED DSVKETDSSA AAVAPSKKKS 

       250        260        270        280        290        300 
LIDQFFGVEF ETTMKCTESE EEEVTKGKEN QLQLSCFINQ EVKYLFTGLK LRLQEEITKQ 

       310        320        330        340        350        360 
SPTLQRNALY IKSSKISRLP AYLTIQMVRF FYKEKESVNA KVLKDVKFPL MLDVYELCTP 

       370        380        390        400        410        420 
ELQEKMVSFR SKFKDIEDKK VNQQPNASDK KSSPQKEVRY EPFSFADDIG SNNCGYYDLQ 

       430        440        450        460        470        480 
AVLTHQGRSS SSGHYVSWVK RKHDEWIKFD DDKVSIVTPE DILRLSGGGD WHIAYVLLYG 

       490 
PRRVEIMEEE NEQ 

« Hide

References

[1]"Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit."
Deshpande K.L., Seubert P.H., Tillman D.M., Farkas W.R., Katze J.R.
Arch. Biochem. Biophys. 326:1-7(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: New Zealand white.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L37420 mRNA. Translation: AAA96133.1.
PIRS68430.
RefSeqNP_001075726.1. NM_001082257.1.
UniGeneOcu.2060.

3D structure databases

ProteinModelPortalP40826.
SMRP40826. Positions 6-86, 98-482.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000015687.

Protein family/group databases

MEROPSC19.015.

Proteomic databases

PRIDEP40826.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSOCUT00000029186; ENSOCUP00000015687; ENSOCUG00000011595.
GeneID100009078.

Organism-specific databases

CTD9097.

Phylogenomic databases

eggNOGNOG286607.
GeneTreeENSGT00390000009615.
HOGENOMHOG000202292.
HOVERGENHBG054185.
OMADWGNLKI.
TreeFamTF314494.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
PROSITEPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP14_RABIT
AccessionPrimary (citable) accession number: P40826
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries