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P40826

- UBP14_RABIT

UniProt

P40826 - UBP14_RABIT

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Protein
Ubiquitin carboxyl-terminal hydrolase 14
Gene
USP14, TGT
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs) By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141Nucleophile By similarity
Active sitei434 – 4341Proton acceptor By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. endopeptidase inhibitor activity Source: Ensembl
  3. ubiquitin thiolesterase activity Source: Ensembl

GO - Biological processi

  1. regulation of chemotaxis Source: Ensembl
  2. regulation of proteasomal protein catabolic process Source: Ensembl
  3. synaptic transmission Source: Ensembl
  4. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:USP14
Synonyms:TGT
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Chromosome 9

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. cytoplasmic membrane-bounded vesicle Source: Ensembl
  3. plasma membrane Source: UniProtKB-SubCell
  4. proteasome complex Source: UniProtKB-KW
  5. synapse Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Ubiquitin carboxyl-terminal hydrolase 14
PRO_0000080639Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei143 – 1431Phosphoserine By similarity
Modified residuei448 – 4481N6-acetyllysine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP40826.

Interactioni

Subunit structurei

Homodimer Reviewed prediction. Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1 By similarity.

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000015687.

Structurei

3D structure databases

ProteinModelPortaliP40826.
SMRiP40826. Positions 6-86, 98-482.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8077Ubiquitin-like
Add
BLAST
Domaini105 – 482378USP
Add
BLAST

Sequence similaritiesi

Contains 1 USP domain.

Phylogenomic databases

eggNOGiNOG286607.
GeneTreeiENSGT00390000009615.
HOGENOMiHOG000202292.
HOVERGENiHBG054185.
OMAiDWGNLKI.
TreeFamiTF314494.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40826-1 [UniParc]FASTAAdd to Basket

« Hide

MPLYSVTVKW GKEKFGGVEL NTDEPPMVFK AQLFALTGVQ PARQRVMVKG    50
GTLKDDDWGN IKIKNGMTIL MMGSADALPE EPSAKTVFVE DMTEEQLASA 100
MELPCGLTNL GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ 150
YITAALRDLF DSMDKTSSSI PPIILLQFLH MAFPQFAEKG EQGQYLQQDA 200
NECWIQMMRV LQQKLEAIED DSVKETDSSA AAVAPSKKKS LIDQFFGVEF 250
ETTMKCTESE EEEVTKGKEN QLQLSCFINQ EVKYLFTGLK LRLQEEITKQ 300
SPTLQRNALY IKSSKISRLP AYLTIQMVRF FYKEKESVNA KVLKDVKFPL 350
MLDVYELCTP ELQEKMVSFR SKFKDIEDKK VNQQPNASDK KSSPQKEVRY 400
EPFSFADDIG SNNCGYYDLQ AVLTHQGRSS SSGHYVSWVK RKHDEWIKFD 450
DDKVSIVTPE DILRLSGGGD WHIAYVLLYG PRRVEIMEEE NEQ 493
Length:493
Mass (Da):55,922
Last modified:January 23, 2007 - v3
Checksum:i598553BF435E6495
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L37420 mRNA. Translation: AAA96133.1.
PIRiS68430.
RefSeqiNP_001075726.1. NM_001082257.1.
UniGeneiOcu.2060.

Genome annotation databases

EnsembliENSOCUT00000029186; ENSOCUP00000015687; ENSOCUG00000011595.
GeneIDi100009078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L37420 mRNA. Translation: AAA96133.1 .
PIRi S68430.
RefSeqi NP_001075726.1. NM_001082257.1.
UniGenei Ocu.2060.

3D structure databases

ProteinModelPortali P40826.
SMRi P40826. Positions 6-86, 98-482.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000015687.

Protein family/group databases

MEROPSi C19.015.

Proteomic databases

PRIDEi P40826.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSOCUT00000029186 ; ENSOCUP00000015687 ; ENSOCUG00000011595 .
GeneIDi 100009078.

Organism-specific databases

CTDi 9097.

Phylogenomic databases

eggNOGi NOG286607.
GeneTreei ENSGT00390000009615.
HOGENOMi HOG000202292.
HOVERGENi HBG054185.
OMAi DWGNLKI.
TreeFami TF314494.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit."
    Deshpande K.L., Seubert P.H., Tillman D.M., Farkas W.R., Katze J.R.
    Arch. Biochem. Biophys. 326:1-7(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: New Zealand white.
    Tissue: Liver.

Entry informationi

Entry nameiUBP14_RABIT
AccessioniPrimary (citable) accession number: P40826
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) thought to be a guanine tRNA-ribosyltransferase.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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