Reviewed,
UniProtKB/Swiss-Prot P40825 (SYAC_YEAST)
Last modified
November 25, 2008.
Version 72.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alanyl-tRNA synthetase, cytoplasmic EC=6.1.1.7 Alternative name(s): Alanine--tRNA ligase Short name=AlaRS | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 958 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). |
| Subcellular location | |
| Miscellaneous | Present with 33800 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | mitochondrion Inferred from direct assay. Source: SGD |
| Molecular function | ATP binding Inferred from electronic annotation. Source: InterPro alanine-tRNA ligase activityInferred from electronic annotation. Source: InterPro nucleic acid bindingInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| RSP5 | P39940 | 1 | EBI-18648,EBI-16219 | |
| SSM4 | P40318 | 1 | EBI-18648,EBI-18208 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 958 | 958 | Alanyl-tRNA synthetase, cytoplasmic | PRO_0000075287 | |||||
Amino acid modifications | |||||||||
| Modified residue | 479 | 1 | Phosphoserine | ||||||
| Modified residue | 950 | 1 | Phosphoserine | ||||||
Experimental info | |||||||||
| Sequence conflict | 136 | 1 | R → S in AAC49007. Ref.1 | ||||||
| Sequence conflict | 465 – 467 | 3 | KDQ → RTK in AAC49007. Ref.1 | ||||||
| Sequence conflict | 840 – 841 | 2 | FE → LQ in AAC49007. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Ripmaster T.L., Schimmel P. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I." Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H. Yeast 12:999-1004(1996) [PubMed: 8896263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K.Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-950, MASS SPECTROMETRY. |
| [6] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479 AND SER-950, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U18672 Genomic DNA. Translation: AAC49007.1. Z49821 Genomic DNA. Translation: CAA89980.1. Z75243 Genomic DNA. Translation: CAA99658.1. | |
| PIR | S62065. |
| RefSeq | NP_014980.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:6286N. |
| IntAct | P40825. |
Proteomic databases | |
| PeptideAtlas | P40825. |
Genome annotation databases | |
| Ensembl | YOR335C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 854513. |
| GenomeReviews | Gene locus YOR335C in contig Y13140_GR. |
| KEGG | sce:YOR335C. |
| NMPDR | fig|4932.3.peg.6096. |
Organism-specific databases | |
| CYGD | YOR335c. |
| SGD | S000005862. ALA1. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P40825. |
Gene expression databases | |
| ArrayExpress | P40825. |
| GermOnline | YOR335C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view] |
| Pfam | PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view] |
| PRINTS | PR00980. TRNASYNTHALA. |
| TIGRFAMs | TIGR00344. alaS. 1 hit. |
| PROSITE | PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P40825. |
| NextBio | 976870. |
Entry information
| Entry name | SYAC_YEAST | ||||||||
| Accession | Primary (citable) accession number: P40825 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


