Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P40815 (T3RE_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type III restriction-modification system StyLTI enzyme res

EC=3.1.21.5
Gene names
Name:res
Ordered Locus Names:STM0358
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length990 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves DNA some 25 base-pairs downstream from the recognition site. May also act as a helicase involved in unwinding DNA at the cleavage site. Protein only required for restriction but needs the presence of the modification enzyme.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Contains two different subunits: res and mod.

Sequence similarities

Belongs to the type III restriction-modification system res protein family.

Contains 1 VRR-NUC domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 990990Type III restriction-modification system StyLTI enzyme res
PRO_0000077375

Regions

Domain884 – 97087VRR-NUC

Experimental info

Sequence conflict171A → R in M90544. Ref.1
Sequence conflict293 – 2986KLILRR → EINPPT in M90544. Ref.1
Sequence conflict3501S → T in M90544. Ref.1
Sequence conflict4201L → V in M90544. Ref.1
Sequence conflict464 – 4652SG → R in M90544. Ref.1
Sequence conflict536 – 5372GL → AV in M90544. Ref.1
Sequence conflict945 – 99046DQVIL…EGKAN → IRLFLMRNVNSSICCVGKIS MSSLRKDQRAGGIFYDQWLD in M90544. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40815 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 8E821E02A895D16E

FASTA990113,388
        10         20         30         40         50         60 
MMNILLEELP HQEQALAAIL ASFTGIDHAQ ADHNHYANPL IKERYDDKAN IDVKMETGTG 

        70         80         90        100        110        120 
KTYVYTRLMY ELHQKYGLFK FVLVVPTPAI KEGARNFITS DYARQHFSQF YENTRMELCT 

       130        140        150        160        170        180 
INAGDFKVKS GRKNFPAQLL SFTDASRRDS HTIQVLLINA QMLNSASMTR DDYDQTLLGG 

       190        200        210        220        230        240 
LTSPVKGLQM TRPVVIIDEP HRFARDNKFY RAIQAIQPQM IVRFGATFPD IVEGKGKNKC 

       250        260        270        280        290        300 
VRKDYYRRQP QFDLNAVDSF NDGLVKGIDI YYPNLPEEQA NNRYIVDSVT AKKLILRRGS 

       310        320        330        340        350        360 
KIAEVGVGEN LADVDAGFEG SIEYAGSKML SNDLELEAGM ALVPGTFGAS YQELIIQDAI 

       370        380        390        400        410        420 
DKHFDTEQAN FLRSNEPENN APRIKTLSLF FIDSIKSYRD DEGWLKVTFE RLLKKKLTQL 

       430        440        450        460        470        480 
IDDYQRKTLP REVEYLSFLQ ATLASLHSDN QNVHAGYFGE DRGSGDEAIQ AEVDDILKNK 

       490        500        510        520        530        540 
EKLLSFSDHH GNWETRRFLF SKWTLREGWD NPNVFVIAKL RSSGSESSKI QEVGRGLRLP 

       550        560        570        580        590        600 
VDENGHRVHQ EEWPSRLSFL IGYDEKAFAS MLVDEINRDS KVQLNEQKLD EAMITLIVTE 

       610        620        630        640        650        660 
RQKVDPAFTE LRLLEDLDDK KLINRSNEFK PSVTLNGETK SGFAWLLEFY PELTQARVRA 

       670        680        690        700        710        720 
DRIRDNKPAS RLRVRLRKEN WEQLSSIWEQ FSRRYMLQFE RSGASLEQIA AEVLRDPALY 

       730        740        750        760        770        780 
IRQKPSQVQQ RLVSNEDNGR FEVAQREGEL AASEFMAGMK YGHFLKQLAL RTSLPVNVLH 

       790        800        810        820        830        840 
PVLMAMLRDV LHGDSRYLSE ISLDNMTRAL QTRINAHFAQ RHDYLPLDFQ ASTSVFDSTA 

       850        860        870        880        890        900 
RQFREEISAE IVGKNVDENA IDDPRSLYQI PPLRYDSVDP ELPLLKYDYP QQVSVFGKLP 

       910        920        930        940        950        960 
KRAIQIPKYT GGSTTPDFVY RIERQDADSV YLLVETKAEN MRVGDQVILD AQRKFFDMLR 

       970        980        990 
RQNINVEFAE ATSAPAVFST INGLIEGKAN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the Salmonella typhimurium StyLT1 restriction-modification genes: homologies with EcoP1 and EcoP15 type-III R-M systems and presence of helicase domains."
Dartois V., de Backer O., Colson C.
Gene 127:105-110(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT7.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M90544 Genomic DNA. No translation available.
AE006468 Genomic DNA. Translation: AAL19312.1.
PIRJN0658.
RefSeqNP_459353.2. NC_003197.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM0358.S.

Protein family/group databases

REBASE5599. StyLT2ORF357P.

Proteomic databases

PaxDbP40815.
PRIDEP40815.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL19312; AAL19312; STM0358.
GeneID1251877.
KEGGstm:STM0358.S.
PATRIC32379035. VBISalEnt20916_0379.

Phylogenomic databases

eggNOGCOG3587.
KOK01156.
OMARESAKTW.
OrthoDBEOG6MPWQ7.

Enzyme and pathway databases

BioCycSENT99287:GCTI-359-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006935. Helicase/UvrB_dom.
IPR027417. P-loop_NTPase.
IPR014883. VRR_NUC.
[Graphical view]
PfamPF04851. ResIII. 1 hit.
PF08774. VRR_NUC. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 4 hits.
ProtoNetSearch...

Entry information

Entry nameT3RE_SALTY
AccessionPrimary (citable) accession number: P40815
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2002
Last modified: May 14, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries