Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P40815

- T3RE_SALTY

UniProt

P40815 - T3RE_SALTY

Protein

Type III restriction-modification system StyLTI enzyme res

Gene

res

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cleaves DNA some 25 base-pairs downstream from the recognition site. May also act as a helicase involved in unwinding DNA at the cleavage site. Protein only required for restriction but needs the presence of the modification enzyme.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. helicase activity Source: UniProtKB-KW
    4. Type III site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-359-MONOMER.

    Protein family/group databases

    REBASEi5599. StyLT2ORF357P.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type III restriction-modification system StyLTI enzyme res (EC:3.1.21.5)
    Gene namesi
    Name:res
    Ordered Locus Names:STM0358
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 990990Type III restriction-modification system StyLTI enzyme resPRO_0000077375Add
    BLAST

    Proteomic databases

    PaxDbiP40815.
    PRIDEiP40815.

    Interactioni

    Subunit structurei

    Contains two different subunits: res and mod.

    Protein-protein interaction databases

    STRINGi99287.STM0358.S.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini884 – 97087VRR-NUCAdd
    BLAST

    Sequence similaritiesi

    Contains 1 VRR-NUC domain.Curated

    Phylogenomic databases

    eggNOGiCOG3587.
    KOiK01156.
    OMAiRESAKTW.
    OrthoDBiEOG6MPWQ7.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR006935. Helicase/UvrB_dom.
    IPR027417. P-loop_NTPase.
    IPR014883. VRR_NUC.
    [Graphical view]
    PfamiPF04851. ResIII. 1 hit.
    PF08774. VRR_NUC. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    P40815-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNILLEELP HQEQALAAIL ASFTGIDHAQ ADHNHYANPL IKERYDDKAN    50
    IDVKMETGTG KTYVYTRLMY ELHQKYGLFK FVLVVPTPAI KEGARNFITS 100
    DYARQHFSQF YENTRMELCT INAGDFKVKS GRKNFPAQLL SFTDASRRDS 150
    HTIQVLLINA QMLNSASMTR DDYDQTLLGG LTSPVKGLQM TRPVVIIDEP 200
    HRFARDNKFY RAIQAIQPQM IVRFGATFPD IVEGKGKNKC VRKDYYRRQP 250
    QFDLNAVDSF NDGLVKGIDI YYPNLPEEQA NNRYIVDSVT AKKLILRRGS 300
    KIAEVGVGEN LADVDAGFEG SIEYAGSKML SNDLELEAGM ALVPGTFGAS 350
    YQELIIQDAI DKHFDTEQAN FLRSNEPENN APRIKTLSLF FIDSIKSYRD 400
    DEGWLKVTFE RLLKKKLTQL IDDYQRKTLP REVEYLSFLQ ATLASLHSDN 450
    QNVHAGYFGE DRGSGDEAIQ AEVDDILKNK EKLLSFSDHH GNWETRRFLF 500
    SKWTLREGWD NPNVFVIAKL RSSGSESSKI QEVGRGLRLP VDENGHRVHQ 550
    EEWPSRLSFL IGYDEKAFAS MLVDEINRDS KVQLNEQKLD EAMITLIVTE 600
    RQKVDPAFTE LRLLEDLDDK KLINRSNEFK PSVTLNGETK SGFAWLLEFY 650
    PELTQARVRA DRIRDNKPAS RLRVRLRKEN WEQLSSIWEQ FSRRYMLQFE 700
    RSGASLEQIA AEVLRDPALY IRQKPSQVQQ RLVSNEDNGR FEVAQREGEL 750
    AASEFMAGMK YGHFLKQLAL RTSLPVNVLH PVLMAMLRDV LHGDSRYLSE 800
    ISLDNMTRAL QTRINAHFAQ RHDYLPLDFQ ASTSVFDSTA RQFREEISAE 850
    IVGKNVDENA IDDPRSLYQI PPLRYDSVDP ELPLLKYDYP QQVSVFGKLP 900
    KRAIQIPKYT GGSTTPDFVY RIERQDADSV YLLVETKAEN MRVGDQVILD 950
    AQRKFFDMLR RQNINVEFAE ATSAPAVFST INGLIEGKAN 990
    Length:990
    Mass (Da):113,388
    Last modified:January 23, 2002 - v2
    Checksum:i8E821E02A895D16E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171A → R in M90544. (PubMed:8387444)Curated
    Sequence conflicti293 – 2986KLILRR → EINPPT in M90544. (PubMed:8387444)Curated
    Sequence conflicti350 – 3501S → T in M90544. (PubMed:8387444)Curated
    Sequence conflicti420 – 4201L → V in M90544. (PubMed:8387444)Curated
    Sequence conflicti464 – 4652SG → R in M90544. (PubMed:8387444)Curated
    Sequence conflicti536 – 5372GL → AV in M90544. (PubMed:8387444)Curated
    Sequence conflicti945 – 99046DQVIL…EGKAN → IRLFLMRNVNSSICCVGKIS MSSLRKDQRAGGIFYDQWLD in M90544. (PubMed:8387444)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90544 Genomic DNA. No translation available.
    AE006468 Genomic DNA. Translation: AAL19312.1.
    PIRiJN0658.
    RefSeqiNP_459353.2. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL19312; AAL19312; STM0358.
    GeneIDi1251877.
    KEGGistm:STM0358.S.
    PATRICi32379035. VBISalEnt20916_0379.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90544 Genomic DNA. No translation available.
    AE006468 Genomic DNA. Translation: AAL19312.1 .
    PIRi JN0658.
    RefSeqi NP_459353.2. NC_003197.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM0358.S.

    Protein family/group databases

    REBASEi 5599. StyLT2ORF357P.

    Proteomic databases

    PaxDbi P40815.
    PRIDEi P40815.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL19312 ; AAL19312 ; STM0358 .
    GeneIDi 1251877.
    KEGGi stm:STM0358.S.
    PATRICi 32379035. VBISalEnt20916_0379.

    Phylogenomic databases

    eggNOGi COG3587.
    KOi K01156.
    OMAi RESAKTW.
    OrthoDBi EOG6MPWQ7.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-359-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR006935. Helicase/UvrB_dom.
    IPR027417. P-loop_NTPase.
    IPR014883. VRR_NUC.
    [Graphical view ]
    Pfami PF04851. ResIII. 1 hit.
    PF08774. VRR_NUC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the Salmonella typhimurium StyLT1 restriction-modification genes: homologies with EcoP1 and EcoP15 type-III R-M systems and presence of helicase domains."
      Dartois V., de Backer O., Colson C.
      Gene 127:105-110(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT7.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.

    Entry informationi

    Entry nameiT3RE_SALTY
    AccessioniPrimary (citable) accession number: P40815
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3