P40815 (T3RE_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type III restriction-modification system StyLTI enzyme res
EC=3.1.21.5

Gene names

Name:	res
Ordered Locus Names:	STM0358

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	990 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves DNA some 25 base-pairs downstream from the recognition site. May also act as a helicase involved in unwinding DNA at the cleavage site. Protein only required for restriction but needs the presence of the modification enzyme.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.
Subunit structure	Contains two different subunits: res and mod.
Sequence similarities	Belongs to the type III restriction-modification system res protein family. Contains 1 VRR-NUC domain.

Ontologies

Keywords
Biological process	Restriction system
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro Type III site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 990	990	Type III restriction-modification system StyLTI enzyme res		PRO_0000077375
Regions
	Domain	884 – 970	87	VRR-NUC
Experimental info
	Sequence conflict	17	1	A → R in M90544. Ref.1
	Sequence conflict	293 – 298	6	KLILRR → EINPPT in M90544. Ref.1
	Sequence conflict	350	1	S → T in M90544. Ref.1
	Sequence conflict	420	1	L → V in M90544. Ref.1
	Sequence conflict	464 – 465	2	SG → R in M90544. Ref.1
	Sequence conflict	536 – 537	2	GL → AV in M90544. Ref.1
	Sequence conflict	945 – 990	46	DQVIL…EGKAN → IRLFLMRNVNSSICCVGKIS MSSLRKDQRAGGIFYDQWLD in M90544. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40815 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 8E821E02A895D16E	FASTA	990	113,388
	10 20 30 40 50 60 MMNILLEELP HQEQALAAIL ASFTGIDHAQ ADHNHYANPL IKERYDDKAN IDVKMETGTG 70 80 90 100 110 120 KTYVYTRLMY ELHQKYGLFK FVLVVPTPAI KEGARNFITS DYARQHFSQF YENTRMELCT 130 140 150 160 170 180 INAGDFKVKS GRKNFPAQLL SFTDASRRDS HTIQVLLINA QMLNSASMTR DDYDQTLLGG 190 200 210 220 230 240 LTSPVKGLQM TRPVVIIDEP HRFARDNKFY RAIQAIQPQM IVRFGATFPD IVEGKGKNKC 250 260 270 280 290 300 VRKDYYRRQP QFDLNAVDSF NDGLVKGIDI YYPNLPEEQA NNRYIVDSVT AKKLILRRGS 310 320 330 340 350 360 KIAEVGVGEN LADVDAGFEG SIEYAGSKML SNDLELEAGM ALVPGTFGAS YQELIIQDAI 370 380 390 400 410 420 DKHFDTEQAN FLRSNEPENN APRIKTLSLF FIDSIKSYRD DEGWLKVTFE RLLKKKLTQL 430 440 450 460 470 480 IDDYQRKTLP REVEYLSFLQ ATLASLHSDN QNVHAGYFGE DRGSGDEAIQ AEVDDILKNK 490 500 510 520 530 540 EKLLSFSDHH GNWETRRFLF SKWTLREGWD NPNVFVIAKL RSSGSESSKI QEVGRGLRLP 550 560 570 580 590 600 VDENGHRVHQ EEWPSRLSFL IGYDEKAFAS MLVDEINRDS KVQLNEQKLD EAMITLIVTE 610 620 630 640 650 660 RQKVDPAFTE LRLLEDLDDK KLINRSNEFK PSVTLNGETK SGFAWLLEFY PELTQARVRA 670 680 690 700 710 720 DRIRDNKPAS RLRVRLRKEN WEQLSSIWEQ FSRRYMLQFE RSGASLEQIA AEVLRDPALY 730 740 750 760 770 780 IRQKPSQVQQ RLVSNEDNGR FEVAQREGEL AASEFMAGMK YGHFLKQLAL RTSLPVNVLH 790 800 810 820 830 840 PVLMAMLRDV LHGDSRYLSE ISLDNMTRAL QTRINAHFAQ RHDYLPLDFQ ASTSVFDSTA 850 860 870 880 890 900 RQFREEISAE IVGKNVDENA IDDPRSLYQI PPLRYDSVDP ELPLLKYDYP QQVSVFGKLP 910 920 930 940 950 960 KRAIQIPKYT GGSTTPDFVY RIERQDADSV YLLVETKAEN MRVGDQVILD AQRKFFDMLR 970 980 990 RQNINVEFAE ATSAPAVFST INGLIEGKAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the Salmonella typhimurium StyLT1 restriction-modification genes: homologies with EcoP1 and EcoP15 type-III R-M systems and presence of helicase domains." Dartois V., de Backer O., Colson C. Gene 127:105-110(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT7.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90544 Genomic DNA. No translation available. AE006468 Genomic DNA. Translation: AAL19312.1.
PIR	JN0658.
RefSeq	NP_459353.2. NC_003197.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM0358.S.
Protein family/group databases
REBASE	5599. StyLT2ORF357P.
Proteomic databases
PaxDb	P40815.
PRIDE	P40815.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL19312; AAL19312; STM0358.
GeneID	1251877.
KEGG	stm:STM0358.S.
PATRIC	32379035. VBISalEnt20916_0379.
Phylogenomic databases
eggNOG	COG3587.
KO	K01156.
OMA	FAWLLEF.
ProtClustDB	PRK15483.
Family and domain databases
InterPro	IPR006935. Helicase/UvrB_dom. IPR014883. VRR_NUC. [Graphical view]
Pfam	PF04851. ResIII. 1 hit. PF08774. VRR_NUC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T3RE_SALTY

Accession

Primary (citable) accession number: P40815

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program