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P40815

- T3RE_SALTY

UniProt

P40815 - T3RE_SALTY

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Protein

Type III restriction-modification system StyLTI enzyme res

Gene

res

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cleaves DNA some 25 base-pairs downstream from the recognition site. May also act as a helicase involved in unwinding DNA at the cleavage site. Protein only required for restriction but needs the presence of the modification enzyme.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. helicase activity Source: UniProtKB-KW
  4. Type III site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-359-MONOMER.

Protein family/group databases

REBASEi5599. StyLT2ORF357P.

Names & Taxonomyi

Protein namesi
Recommended name:
Type III restriction-modification system StyLTI enzyme res (EC:3.1.21.5)
Gene namesi
Name:res
Ordered Locus Names:STM0358
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 990990Type III restriction-modification system StyLTI enzyme resPRO_0000077375Add
BLAST

Proteomic databases

PaxDbiP40815.
PRIDEiP40815.

Interactioni

Subunit structurei

Contains two different subunits: res and mod.

Protein-protein interaction databases

STRINGi99287.STM0358.S.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini884 – 97087VRR-NUCAdd
BLAST

Sequence similaritiesi

Contains 1 VRR-NUC domain.Curated

Phylogenomic databases

eggNOGiCOG3587.
KOiK01156.
OMAiRESAKTW.
OrthoDBiEOG6MPWQ7.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006935. Helicase/UvrB_dom.
IPR027417. P-loop_NTPase.
IPR014883. VRR_NUC.
[Graphical view]
PfamiPF04851. ResIII. 1 hit.
PF08774. VRR_NUC. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.

Sequencei

Sequence statusi: Complete.

P40815-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMNILLEELP HQEQALAAIL ASFTGIDHAQ ADHNHYANPL IKERYDDKAN
60 70 80 90 100
IDVKMETGTG KTYVYTRLMY ELHQKYGLFK FVLVVPTPAI KEGARNFITS
110 120 130 140 150
DYARQHFSQF YENTRMELCT INAGDFKVKS GRKNFPAQLL SFTDASRRDS
160 170 180 190 200
HTIQVLLINA QMLNSASMTR DDYDQTLLGG LTSPVKGLQM TRPVVIIDEP
210 220 230 240 250
HRFARDNKFY RAIQAIQPQM IVRFGATFPD IVEGKGKNKC VRKDYYRRQP
260 270 280 290 300
QFDLNAVDSF NDGLVKGIDI YYPNLPEEQA NNRYIVDSVT AKKLILRRGS
310 320 330 340 350
KIAEVGVGEN LADVDAGFEG SIEYAGSKML SNDLELEAGM ALVPGTFGAS
360 370 380 390 400
YQELIIQDAI DKHFDTEQAN FLRSNEPENN APRIKTLSLF FIDSIKSYRD
410 420 430 440 450
DEGWLKVTFE RLLKKKLTQL IDDYQRKTLP REVEYLSFLQ ATLASLHSDN
460 470 480 490 500
QNVHAGYFGE DRGSGDEAIQ AEVDDILKNK EKLLSFSDHH GNWETRRFLF
510 520 530 540 550
SKWTLREGWD NPNVFVIAKL RSSGSESSKI QEVGRGLRLP VDENGHRVHQ
560 570 580 590 600
EEWPSRLSFL IGYDEKAFAS MLVDEINRDS KVQLNEQKLD EAMITLIVTE
610 620 630 640 650
RQKVDPAFTE LRLLEDLDDK KLINRSNEFK PSVTLNGETK SGFAWLLEFY
660 670 680 690 700
PELTQARVRA DRIRDNKPAS RLRVRLRKEN WEQLSSIWEQ FSRRYMLQFE
710 720 730 740 750
RSGASLEQIA AEVLRDPALY IRQKPSQVQQ RLVSNEDNGR FEVAQREGEL
760 770 780 790 800
AASEFMAGMK YGHFLKQLAL RTSLPVNVLH PVLMAMLRDV LHGDSRYLSE
810 820 830 840 850
ISLDNMTRAL QTRINAHFAQ RHDYLPLDFQ ASTSVFDSTA RQFREEISAE
860 870 880 890 900
IVGKNVDENA IDDPRSLYQI PPLRYDSVDP ELPLLKYDYP QQVSVFGKLP
910 920 930 940 950
KRAIQIPKYT GGSTTPDFVY RIERQDADSV YLLVETKAEN MRVGDQVILD
960 970 980 990
AQRKFFDMLR RQNINVEFAE ATSAPAVFST INGLIEGKAN
Length:990
Mass (Da):113,388
Last modified:January 23, 2002 - v2
Checksum:i8E821E02A895D16E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171A → R in M90544. (PubMed:8387444)Curated
Sequence conflicti293 – 2986KLILRR → EINPPT in M90544. (PubMed:8387444)Curated
Sequence conflicti350 – 3501S → T in M90544. (PubMed:8387444)Curated
Sequence conflicti420 – 4201L → V in M90544. (PubMed:8387444)Curated
Sequence conflicti464 – 4652SG → R in M90544. (PubMed:8387444)Curated
Sequence conflicti536 – 5372GL → AV in M90544. (PubMed:8387444)Curated
Sequence conflicti945 – 99046DQVIL…EGKAN → IRLFLMRNVNSSICCVGKIS MSSLRKDQRAGGIFYDQWLD in M90544. (PubMed:8387444)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90544 Genomic DNA. No translation available.
AE006468 Genomic DNA. Translation: AAL19312.1.
PIRiJN0658.
RefSeqiNP_459353.2. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19312; AAL19312; STM0358.
GeneIDi1251877.
KEGGistm:STM0358.S.
PATRICi32379035. VBISalEnt20916_0379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90544 Genomic DNA. No translation available.
AE006468 Genomic DNA. Translation: AAL19312.1 .
PIRi JN0658.
RefSeqi NP_459353.2. NC_003197.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM0358.S.

Protein family/group databases

REBASEi 5599. StyLT2ORF357P.

Proteomic databases

PaxDbi P40815.
PRIDEi P40815.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL19312 ; AAL19312 ; STM0358 .
GeneIDi 1251877.
KEGGi stm:STM0358.S.
PATRICi 32379035. VBISalEnt20916_0379.

Phylogenomic databases

eggNOGi COG3587.
KOi K01156.
OMAi RESAKTW.
OrthoDBi EOG6MPWQ7.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-359-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR006935. Helicase/UvrB_dom.
IPR027417. P-loop_NTPase.
IPR014883. VRR_NUC.
[Graphical view ]
Pfami PF04851. ResIII. 1 hit.
PF08774. VRR_NUC. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the Salmonella typhimurium StyLT1 restriction-modification genes: homologies with EcoP1 and EcoP15 type-III R-M systems and presence of helicase domains."
    Dartois V., de Backer O., Colson C.
    Gene 127:105-110(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT7.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiT3RE_SALTY
AccessioniPrimary (citable) accession number: P40815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2002
Last modified: October 1, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3