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P40814 (T3MO_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type III restriction-modification system StyLTI enzyme mod

Short name=M.StyLTI
EC=2.1.1.72
Alternative name(s):
StyLTI methyltransferase
Gene names
Name:mod
Ordered Locus Names:STM0357
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length652 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Binds the system-specific DNA recognition site 5'-CAGAG-3'. Necessary for restriction and for methylation of A-4.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Contains two different subunits: res and mod. Mod is a homotetramer By similarity.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 652652Type III restriction-modification system StyLTI enzyme mod
PRO_0000088032

Regions

Region135 – 1384Binding of S-adenosyl methionine Potential

Experimental info

Sequence conflict551Missing Ref.1
Sequence conflict1261A → S Ref.1
Sequence conflict587 – 5882DN → ND Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40814 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 385DE4851545C84E

FASTA65273,336
        10         20         30         40         50         60 
MLKDNQKHNE SVAPNSAFLS ELQRALPEFF TADRYNEQGE LIAKGGFDLA RFERALKARN 

        70         80         90        100        110        120 
IDELTSGYQI DFIGKDYAKK QAGEKSVTVI VPDVEHNTLA ENKNSHNLFL TGDNLDVLRH 

       130        140        150        160        170        180 
LQNNYADTVD MIYIDPPYNT GSDGFVYPDH FEYSDRALQD MFGLNDTELA RLKSIQGKST 

       190        200        210        220        230        240 
HSAWLSFMYP RLFLARKLLK DTGFIFISID DNEYANLKLM MDEIFGEGGF VTNVMWKRKK 

       250        260        270        280        290        300 
EISNDSDNVS IQGEYILVYA KTGQGALRLE PLSKEYIQKS YKEPTEQFPE GKWRPVPLTV 

       310        320        330        340        350        360 
SKGLSGGGYT YKITTPNGTV HERLWAYPEA SYQKLVADNL VYFGKDNGGI PQRVMYAHHS 

       370        380        390        400        410        420 
KGQPTTNYWD NVASNKEGKK EILDLFGDNV FDTPKPTALL KKIIKLAIDK DGVVLDFFAG 

       430        440        450        460        470        480 
SGTTAHAVMA LNEEDGGQRT FILCTIDQAL SNNTIAKKAG YNTIDEISRE RITRVAAKIR 

       490        500        510        520        530        540 
ANNPATNSDL GFKHYRFATP TQQTLDDLDS FDIATGHFIN TSGQLAAFTE SGFTDMINPF 

       550        560        570        580        590        600 
SARGLGVPGG ASGEETLLTT WLVADGYKMD IDVQTVDFSG YCARYVDNTR LYLIDERWGT 

       610        620        630        640        650 
EQTRDLLNHI GTHQLPVQTI VIYGYSFDLE SIRELEIGLK QLDQKVNLVK RY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the Salmonella typhimurium StyLT1 restriction-modification genes: homologies with EcoP1 and EcoP15 type-III R-M systems and presence of helicase domains."
Dartois V., de Backer O., Colson C.
Gene 127:105-110(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT7.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M90544 Genomic DNA. No translation available.
AE006468 Genomic DNA. Translation: AAL19311.1.
PIRJN0657.
RefSeqNP_459352.1. NC_003197.1.

3D structure databases

ProteinModelPortalP40814.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM0357.

Protein family/group databases

REBASE5598. M.StyLT2ORF357P.

Proteomic databases

PaxDbP40814.
PRIDEP40814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL19311; AAL19311; STM0357.
GeneID1251876.
KEGGstm:STM0357.
PATRIC32379033. VBISalEnt20916_0378.

Phylogenomic databases

eggNOGCOG2189.
HOGENOMHOG000253523.
KOK07316.
OMAYLANCHE.
OrthoDBEOG6W9X8K.

Enzyme and pathway databases

BioCycSENT99287:GCTI-358-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 3 hits.
InterProIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSPR00506. D21N6MTFRASE.
SUPFAMSSF53335. SSF53335. 3 hits.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT3MO_SALTY
AccessionPrimary (citable) accession number: P40814
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2002
Last modified: June 11, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries