Reviewed, UniProtKB/Swiss-Prot P40814 (T3MO_SALTY)

Names and origin

Protein names

Recommended name:
    Type III restriction-modification system StyLTI enzyme mod
      Short name=M.StyLTI
    EC=2.1.1.72
Alternative name(s):
    StyLTI methyltransferase

Gene names

Name:	mod
Ordered Locus Names:	STM0357

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Binds the system-specific DNA recognition site 5'-CAGAG-3'. Necessary for restriction and for methylation of A-4.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Contains two different subunits: res and mod. Mod is a homotetramer By similarity.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	Type III restriction-modification system StyLTI enzyme mod		PRO_0000088032
Regions
	Region	135 – 138	4	Binding of S-adenosyl methionine Potential
Experimental info
	Sequence conflict	55	1	Missing Ref.1
	Sequence conflict	126	1	A → S Ref.1
	Sequence conflict	587 – 588	2	DN → ND Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40814-1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 385DE4851545C84E	FASTA	652	73,336
	10 20 30 40 50 60 MLKDNQKHNE SVAPNSAFLS ELQRALPEFF TADRYNEQGE LIAKGGFDLA RFERALKARN 70 80 90 100 110 120 IDELTSGYQI DFIGKDYAKK QAGEKSVTVI VPDVEHNTLA ENKNSHNLFL TGDNLDVLRH 130 140 150 160 170 180 LQNNYADTVD MIYIDPPYNT GSDGFVYPDH FEYSDRALQD MFGLNDTELA RLKSIQGKST 190 200 210 220 230 240 HSAWLSFMYP RLFLARKLLK DTGFIFISID DNEYANLKLM MDEIFGEGGF VTNVMWKRKK 250 260 270 280 290 300 EISNDSDNVS IQGEYILVYA KTGQGALRLE PLSKEYIQKS YKEPTEQFPE GKWRPVPLTV 310 320 330 340 350 360 SKGLSGGGYT YKITTPNGTV HERLWAYPEA SYQKLVADNL VYFGKDNGGI PQRVMYAHHS 370 380 390 400 410 420 KGQPTTNYWD NVASNKEGKK EILDLFGDNV FDTPKPTALL KKIIKLAIDK DGVVLDFFAG 430 440 450 460 470 480 SGTTAHAVMA LNEEDGGQRT FILCTIDQAL SNNTIAKKAG YNTIDEISRE RITRVAAKIR 490 500 510 520 530 540 ANNPATNSDL GFKHYRFATP TQQTLDDLDS FDIATGHFIN TSGQLAAFTE SGFTDMINPF 550 560 570 580 590 600 SARGLGVPGG ASGEETLLTT WLVADGYKMD IDVQTVDFSG YCARYVDNTR LYLIDERWGT 610 620 630 640 650 EQTRDLLNHI GTHQLPVQTI VIYGYSFDLE SIRELEIGLK QLDQKVNLVK RY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the Salmonella typhimurium StyLT1 restriction-modification genes: homologies with EcoP1 and EcoP15 type-III R-M systems and presence of helicase domains." Dartois V., de Backer O., Colson C. Gene 127:105-110(1993) [PubMed: 8387444] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT7.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90544 Genomic DNA. No translation available. AE006468 Genomic DNA. Translation: AAL19311.1.
PIR	JN0657.
RefSeq	NP_459352.1.
3D structure databases
SMR	P40814. Positions 105-478.
ModBase	Search...
Protein family/group databases
REBASE	5598. M.StyLT2ORF357P.
Proteomic databases
PRIDE	P40814.
Genome annotation databases
GeneID	1251876.
GenomeReviews	Gene locus STM0357 in contig AE006468_GR.
KEGG	stm:STM0357.
NMPDR	fig|99287.1.peg.343.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734529.
OMA	IDVQTID.
Enzyme and pathway databases
BioCyc	STYP99287:STM0357-MONOMER.
BRENDA	2.1.1.72. 2.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T3MO_SALTY

Accession

Primary (citable) accession number: P40814

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2002
Last modified:	January 19, 2010
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)