P40813 (T1M_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type I restriction enzyme StySJI M protein
Short name=M.StySJI
EC=2.1.1.72

Gene names

Name:	hsdM
Synonyms:	hsdT
Ordered Locus Names:	STM4525

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylation of specific adenine residues; required for both restriction and modification activities By similarity. The StySJI enzyme recognizes 5'-GAGN6GTRC-3'.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S.
Miscellaneous	Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg2+ as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Type I restriction enzyme StySJI M protein		PRO_0000088026
Regions
	Region	148 – 153	6	S-adenosyl-L-methionine binding By similarity
	Region	178 – 180	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	216	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40813 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D5EF542069B8A88A	FASTA	529	59,311
	10 20 30 40 50 60 MNNNDLVAKL WKLCDNLRDG GVSYQNYVNE LASLLFLKMC KETGQEADYL PEGYRWDDLK 70 80 90 100 110 120 SRIGQDQMQF YRNLLVQLGS DEKKLVQAVF HNVSTTIEQP KQLTELVSYM DALDWYNGNH 130 140 150 160 170 180 GKSRDDFGDM YEGLLQKNAN ETKSGAGQYF TPRPLIKTII HLLKPQPREV VQDPAAGTAG 190 200 210 220 230 240 FLIEADRYVK SQTNDLDDLD GDTQDFQIHR AFIGLELVPG TRRLALMNCL LHDIEGNLDH 250 260 270 280 290 300 GGAIRLGNTL GSDGENLPKA HIVATNPPFG SAAGTNITRT FVHPTSNKQL CFMQHIIETL 310 320 330 340 350 360 HPGGRAAVVV PDNVLFEGGK GTDIRRDLMD KCHLHTILRL PTGIFYAQGV KTNVLFFTKG 370 380 390 400 410 420 TVTNPHQDKN CTDDVWVYDL RTNMPSFGKR TPFTEQHLQP FETVYGEDPH GLSPREEGEW 430 440 450 460 470 480 SFNAEESEVA DSEENKNTDQ HQATSRWRKF SREWIRSAKS DSLDISWLKD KDSIDADSLP 490 500 510 520 EPDVLAAEAM GELVQALGEL DALMRELGAG DEADAQRQLL NEAFGEVKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Roles of selection and recombination in the evolution of type I restriction-modification systems in enterobacteria." Sharp P.M., Kelleher J.E., Daniel A.S., Cowan G.M., Murray N.E. Proc. Natl. Acad. Sci. U.S.A. 89:9836-9840(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02506 Unassigned DNA. Translation: AAA19429.1. AE006468 Genomic DNA. Translation: AAL23343.1.
RefSeq	NP_463384.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P40813.
SMR	P40813. Positions 4-525.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM4525.
Protein family/group databases
REBASE	5596. M.StyLT2ORF4525P.
Proteomic databases
PaxDb	P40813.
PRIDE	P40813.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL23343; AAL23343; STM4525.
GeneID	1256051.
KEGG	stm:STM4525.
PATRIC	32388011. VBISalEnt20916_4768.
Phylogenomic databases
eggNOG	COG0286.
HOGENOM	HOG000295041.
KO	K03427.
OMA	IVQKLWN.
ProtClustDB	CLSK891832.
Enzyme and pathway databases
BioCyc	SENT99287:GCTI-4557-MONOMER.
Family and domain databases
InterPro	IPR022749. D12N6_MeTrfase_N. IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
Pfam	PF12161. HsdM_N. 1 hit. PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1M_SALTY

Accession

Primary (citable) accession number: P40813

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program