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P40813

- T1M_SALTY

UniProt

P40813 - T1M_SALTY

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Protein
Type I restriction enzyme StySJI M protein
Gene
hsdM, hsdT, STM4525
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylation of specific adenine residues; required for both restriction and modification activities By similarity. The StySJI enzyme recognizes 5'-GAGN6GTRC-3'.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei216 – 2161S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSENT99287:GCTI-4557-MONOMER.

Protein family/group databases

REBASEi5596. M.StyLT2ORF4525P.

Names & Taxonomyi

Protein namesi
Recommended name:
Type I restriction enzyme StySJI M protein (EC:2.1.1.72)
Short name:
M.StySJI
Gene namesi
Name:hsdM
Synonyms:hsdT
Ordered Locus Names:STM4525
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Type I restriction enzyme StySJI M protein
PRO_0000088026Add
BLAST

Proteomic databases

PaxDbiP40813.
PRIDEiP40813.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Protein-protein interaction databases

STRINGi99287.STM4525.

Structurei

3D structure databases

ProteinModelPortaliP40813.
SMRiP40813. Positions 4-525.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1536S-adenosyl-L-methionine binding By similarity
Regioni178 – 1803S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0286.
HOGENOMiHOG000295041.
KOiK03427.
OMAiCDVLRDD.
OrthoDBiEOG686NCV.
PhylomeDBiP40813.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40813-1 [UniParc]FASTAAdd to Basket

« Hide

MNNNDLVAKL WKLCDNLRDG GVSYQNYVNE LASLLFLKMC KETGQEADYL    50
PEGYRWDDLK SRIGQDQMQF YRNLLVQLGS DEKKLVQAVF HNVSTTIEQP 100
KQLTELVSYM DALDWYNGNH GKSRDDFGDM YEGLLQKNAN ETKSGAGQYF 150
TPRPLIKTII HLLKPQPREV VQDPAAGTAG FLIEADRYVK SQTNDLDDLD 200
GDTQDFQIHR AFIGLELVPG TRRLALMNCL LHDIEGNLDH GGAIRLGNTL 250
GSDGENLPKA HIVATNPPFG SAAGTNITRT FVHPTSNKQL CFMQHIIETL 300
HPGGRAAVVV PDNVLFEGGK GTDIRRDLMD KCHLHTILRL PTGIFYAQGV 350
KTNVLFFTKG TVTNPHQDKN CTDDVWVYDL RTNMPSFGKR TPFTEQHLQP 400
FETVYGEDPH GLSPREEGEW SFNAEESEVA DSEENKNTDQ HQATSRWRKF 450
SREWIRSAKS DSLDISWLKD KDSIDADSLP EPDVLAAEAM GELVQALGEL 500
DALMRELGAG DEADAQRQLL NEAFGEVKA 529
Length:529
Mass (Da):59,311
Last modified:February 1, 1995 - v1
Checksum:iD5EF542069B8A88A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L02506 Unassigned DNA. Translation: AAA19429.1.
AE006468 Genomic DNA. Translation: AAL23343.1.
RefSeqiNP_463384.1. NC_003197.1.
WP_001063190.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL23343; AAL23343; STM4525.
GeneIDi1256051.
KEGGistm:STM4525.
PATRICi32388011. VBISalEnt20916_4768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L02506 Unassigned DNA. Translation: AAA19429.1 .
AE006468 Genomic DNA. Translation: AAL23343.1 .
RefSeqi NP_463384.1. NC_003197.1.
WP_001063190.1. NC_003197.1.

3D structure databases

ProteinModelPortali P40813.
SMRi P40813. Positions 4-525.
ModBasei Search...

Protein-protein interaction databases

STRINGi 99287.STM4525.

Protein family/group databases

REBASEi 5596. M.StyLT2ORF4525P.

Proteomic databases

PaxDbi P40813.
PRIDEi P40813.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL23343 ; AAL23343 ; STM4525 .
GeneIDi 1256051.
KEGGi stm:STM4525.
PATRICi 32388011. VBISalEnt20916_4768.

Phylogenomic databases

eggNOGi COG0286.
HOGENOMi HOG000295041.
KOi K03427.
OMAi CDVLRDD.
OrthoDBi EOG686NCV.
PhylomeDBi P40813.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-4557-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Roles of selection and recombination in the evolution of type I restriction-modification systems in enterobacteria."
    Sharp P.M., Kelleher J.E., Daniel A.S., Cowan G.M., Murray N.E.
    Proc. Natl. Acad. Sci. U.S.A. 89:9836-9840(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiT1M_SALTY
AccessioniPrimary (citable) accession number: P40813
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg2+ as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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