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P40811

- ILVI_SALTY

UniProt

P40811 - ILVI_SALTY

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Protein
Acetolactate synthase isozyme 3 large subunit
Gene
ilvI, STM0116
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 thiamine pyrophosphate per subunit By similarity.

Enzyme regulationi

Sensitive to valine inhibition.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511Thiamine pyrophosphate By similarity
Binding sitei153 – 1531FAD By similarity
Metal bindingi448 – 4481Magnesium By similarity
Metal bindingi475 – 4751Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi261 – 28222FAD By similarity
Add
BLAST
Nucleotide bindingi304 – 32320FAD By similarity
Add
BLAST

GO - Molecular functioni

  1. acetolactate synthase activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro
  3. magnesium ion binding Source: InterPro
  4. thiamine pyrophosphate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. isoleucine biosynthetic process Source: UniProtKB-UniPathway
  2. valine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciSENT99287:GCTI-114-MONOMER.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 3 large subunit (EC:2.2.1.6)
Alternative name(s):
AHAS-III
ALS-III
Acetohydroxy-acid synthase III large subunit
Gene namesi
Name:ilvI
Ordered Locus Names:STM0116
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Acetolactate synthase isozyme 3 large subunit
PRO_0000090790Add
BLAST

Proteomic databases

PaxDbiP40811.
PRIDEiP40811.

Interactioni

Subunit structurei

Dimer of chain H and chain I.

Protein-protein interaction databases

STRINGi99287.STM0116.

Structurei

3D structure databases

ProteinModelPortaliP40811.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni397 – 47781Thiamine pyrophosphate binding
Add
BLAST

Sequence similaritiesi

Belongs to the TPP enzyme family.

Phylogenomic databases

eggNOGiCOG0028.
HOGENOMiHOG000258448.
KOiK01652.
OMAiEETVICV.
OrthoDBiEOG6KT2NW.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40811-1 [UniParc]FASTAAdd to Basket

« Hide

MEMLSGAEMV VRSLIDQGVK QVFGYPGGAV LDIYDALHTV GGIDHVLVRH    50
EQAAVHMADG LARATGDVGV VLVTSGPGAT NAITGIATAY MDSIPLVILS 100
GQVATSLIGY DAFQECDMVG ISRPVVKHSF LVKQTEDIPL VLKKAFWLAA 150
SGRPGPVVVD LPKDILNPAK KMPYAWPETV SMRSYNPTTS GHKGQIKRAL 200
QTLASAKKPV VYVGGGAISA ACYAPLRHII ETFNLPVVSS LMGLGAFPAT 250
HRQSLGMLGM HGTYEANMTM HNADVIFAVG VRFDDRTTNN LAKYCPNATV 300
LHIDIDPTSI SKTVNADIPV VGDARLVLEQ MLELLAQDAP SQPQDDIRDW 350
WQQIESWRAR QCLKYDAESE SIKPQAVIET LWRLTKGDAY VTSDVGQHQM 400
FAALYYPFDK PRRWINSGGL GTMGFGLPAA LGVKMALPKE MVVCVTGDGS 450
IQMNIQELST ALQYELPVLV LNLNNRYLGM VKQWQDMIYS GRHSQSYMQS 500
LPDFVRLAEA YGHVGLQINR PDELESKLSE ALEHVRNNRL VFVDVTVDGS 550
EHVYPMQIRG GGMDEMWLSK TERT 574
Length:574
Mass (Da):62,896
Last modified:December 19, 2001 - v3
Checksum:i72EB67FA2667B398
GO

Sequence cautioni

The sequence AAL19080.1 differs from that shown. Reason: Erroneous termination at position 12. Translated as Arg.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL19080.1. Sequence problems.
X55456 Genomic DNA. Translation: CAA39101.1.
PIRiS15939.
RefSeqiNP_459121.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19080; AAL19080; STM0116.
GeneIDi1251634.
KEGGistm:STM0116.
PATRICi32378503. VBISalEnt20916_0121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL19080.1 . Sequence problems.
X55456 Genomic DNA. Translation: CAA39101.1 .
PIRi S15939.
RefSeqi NP_459121.1. NC_003197.1.

3D structure databases

ProteinModelPortali P40811.
ModBasei Search...

Protein-protein interaction databases

STRINGi 99287.STM0116.

Proteomic databases

PaxDbi P40811.
PRIDEi P40811.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL19080 ; AAL19080 ; STM0116 .
GeneIDi 1251634.
KEGGi stm:STM0116.
PATRICi 32378503. VBISalEnt20916_0121.

Phylogenomic databases

eggNOGi COG0028.
HOGENOMi HOG000258448.
KOi K01652.
OMAi EETVICV.
OrthoDBi EOG6KT2NW.

Enzyme and pathway databases

UniPathwayi UPA00047 ; UER00055 .
UPA00049 ; UER00059 .
BioCyci SENT99287:GCTI-114-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProi IPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00118. acolac_lg. 1 hit.
PROSITEi PS00187. TPP_ENZYMES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2."
    Jahreis K., Postma P.W., Lengeler J.W.
    Mol. Gen. Genet. 226:332-336(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 516-574.
    Strain: LT2.

Entry informationi

Entry nameiILVI_SALTY
AccessioniPrimary (citable) accession number: P40811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 19, 2001
Last modified: June 11, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

S.typhimurium contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.
Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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