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P40811

- ILVI_SALTY

UniProt

P40811 - ILVI_SALTY

Protein

Acetolactate synthase isozyme 3 large subunit

Gene

ilvI

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (19 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 pyruvate = 2-acetolactate + CO2.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 thiamine pyrophosphate per subunit.By similarity

    Enzyme regulationi

    Sensitive to valine inhibition.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511Thiamine pyrophosphateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Metal bindingi448 – 4481MagnesiumBy similarity
    Metal bindingi475 – 4751MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi261 – 28222FADBy similarityAdd
    BLAST
    Nucleotide bindingi304 – 32320FADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. acetolactate synthase activity Source: UniProtKB-EC
    2. flavin adenine dinucleotide binding Source: InterPro
    3. magnesium ion binding Source: InterPro
    4. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. isoleucine biosynthetic process Source: UniProtKB-UniPathway
    2. valine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-114-MONOMER.
    UniPathwayiUPA00047; UER00055.
    UPA00049; UER00059.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetolactate synthase isozyme 3 large subunit (EC:2.2.1.6)
    Alternative name(s):
    AHAS-III
    ALS-III
    Acetohydroxy-acid synthase III large subunit
    Gene namesi
    Name:ilvI
    Ordered Locus Names:STM0116
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Acetolactate synthase isozyme 3 large subunitPRO_0000090790Add
    BLAST

    Proteomic databases

    PaxDbiP40811.
    PRIDEiP40811.

    Interactioni

    Subunit structurei

    Dimer of chain H and chain I.

    Protein-protein interaction databases

    STRINGi99287.STM0116.

    Structurei

    3D structure databases

    ProteinModelPortaliP40811.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni397 – 47781Thiamine pyrophosphate bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TPP enzyme family.Curated

    Phylogenomic databases

    eggNOGiCOG0028.
    HOGENOMiHOG000258448.
    KOiK01652.
    OMAiEETVICV.
    OrthoDBiEOG6KT2NW.

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
    PROSITEiPS00187. TPP_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40811-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEMLSGAEMV VRSLIDQGVK QVFGYPGGAV LDIYDALHTV GGIDHVLVRH    50
    EQAAVHMADG LARATGDVGV VLVTSGPGAT NAITGIATAY MDSIPLVILS 100
    GQVATSLIGY DAFQECDMVG ISRPVVKHSF LVKQTEDIPL VLKKAFWLAA 150
    SGRPGPVVVD LPKDILNPAK KMPYAWPETV SMRSYNPTTS GHKGQIKRAL 200
    QTLASAKKPV VYVGGGAISA ACYAPLRHII ETFNLPVVSS LMGLGAFPAT 250
    HRQSLGMLGM HGTYEANMTM HNADVIFAVG VRFDDRTTNN LAKYCPNATV 300
    LHIDIDPTSI SKTVNADIPV VGDARLVLEQ MLELLAQDAP SQPQDDIRDW 350
    WQQIESWRAR QCLKYDAESE SIKPQAVIET LWRLTKGDAY VTSDVGQHQM 400
    FAALYYPFDK PRRWINSGGL GTMGFGLPAA LGVKMALPKE MVVCVTGDGS 450
    IQMNIQELST ALQYELPVLV LNLNNRYLGM VKQWQDMIYS GRHSQSYMQS 500
    LPDFVRLAEA YGHVGLQINR PDELESKLSE ALEHVRNNRL VFVDVTVDGS 550
    EHVYPMQIRG GGMDEMWLSK TERT 574
    Length:574
    Mass (Da):62,896
    Last modified:December 19, 2001 - v3
    Checksum:i72EB67FA2667B398
    GO

    Sequence cautioni

    The sequence AAL19080.1 differs from that shown. Reason: Erroneous termination at position 12. Translated as Arg.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19080.1. Sequence problems.
    X55456 Genomic DNA. Translation: CAA39101.1.
    PIRiS15939.
    RefSeqiNP_459121.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL19080; AAL19080; STM0116.
    GeneIDi1251634.
    KEGGistm:STM0116.
    PATRICi32378503. VBISalEnt20916_0121.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19080.1 . Sequence problems.
    X55456 Genomic DNA. Translation: CAA39101.1 .
    PIRi S15939.
    RefSeqi NP_459121.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P40811.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM0116.

    Proteomic databases

    PaxDbi P40811.
    PRIDEi P40811.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL19080 ; AAL19080 ; STM0116 .
    GeneIDi 1251634.
    KEGGi stm:STM0116.
    PATRICi 32378503. VBISalEnt20916_0121.

    Phylogenomic databases

    eggNOGi COG0028.
    HOGENOMi HOG000258448.
    KOi K01652.
    OMAi EETVICV.
    OrthoDBi EOG6KT2NW.

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00055 .
    UPA00049 ; UER00059 .
    BioCyci SENT99287:GCTI-114-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProi IPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view ]
    Pfami PF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00118. acolac_lg. 1 hit.
    PROSITEi PS00187. TPP_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2."
      Jahreis K., Postma P.W., Lengeler J.W.
      Mol. Gen. Genet. 226:332-336(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 516-574.
      Strain: LT2.

    Entry informationi

    Entry nameiILVI_SALTY
    AccessioniPrimary (citable) accession number: P40811
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: December 19, 2001
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    S.typhimurium contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.
    Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3