P40811 (ILVI_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase isozyme 3 large subunit
EC=2.2.1.6

Alternative name(s):
AHAS-III
ALS-III
Acetohydroxy-acid synthase III large subunit

Gene names

Name:	ilvI
Ordered Locus Names:	STM0116

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Enzyme regulation	Sensitive to valine inhibition.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of chain H and chain I.
Miscellaneous	S.typhimurium contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH. Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry By similarity.
Sequence similarities	Belongs to the TPP enzyme family.
Sequence caution	The sequence AAL19080.1 differs from that shown. Reason: Erroneous termination at position 12. Translated as Arg.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway valine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Acetolactate synthase isozyme 3 large subunit		PRO_0000090790
Regions
	Nucleotide binding	261 – 282	22	FAD By similarity
	Nucleotide binding	304 – 323	20	FAD By similarity
	Region	397 – 477	81	Thiamine pyrophosphate binding
Sites
	Metal binding	448	1	Magnesium By similarity
	Metal binding	475	1	Magnesium By similarity
	Binding site	51	1	Thiamine pyrophosphate By similarity
	Binding site	153	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40811 [UniParc]. Last modified December 19, 2001. Version 3. Checksum: 72EB67FA2667B398	FASTA	574	62,896
	10 20 30 40 50 60 MEMLSGAEMV VRSLIDQGVK QVFGYPGGAV LDIYDALHTV GGIDHVLVRH EQAAVHMADG 70 80 90 100 110 120 LARATGDVGV VLVTSGPGAT NAITGIATAY MDSIPLVILS GQVATSLIGY DAFQECDMVG 130 140 150 160 170 180 ISRPVVKHSF LVKQTEDIPL VLKKAFWLAA SGRPGPVVVD LPKDILNPAK KMPYAWPETV 190 200 210 220 230 240 SMRSYNPTTS GHKGQIKRAL QTLASAKKPV VYVGGGAISA ACYAPLRHII ETFNLPVVSS 250 260 270 280 290 300 LMGLGAFPAT HRQSLGMLGM HGTYEANMTM HNADVIFAVG VRFDDRTTNN LAKYCPNATV 310 320 330 340 350 360 LHIDIDPTSI SKTVNADIPV VGDARLVLEQ MLELLAQDAP SQPQDDIRDW WQQIESWRAR 370 380 390 400 410 420 QCLKYDAESE SIKPQAVIET LWRLTKGDAY VTSDVGQHQM FAALYYPFDK PRRWINSGGL 430 440 450 460 470 480 GTMGFGLPAA LGVKMALPKE MVVCVTGDGS IQMNIQELST ALQYELPVLV LNLNNRYLGM 490 500 510 520 530 540 VKQWQDMIYS GRHSQSYMQS LPDFVRLAEA YGHVGLQINR PDELESKLSE ALEHVRNNRL 550 560 570 VFVDVTVDGS EHVYPMQIRG GGMDEMWLSK TERT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2." Jahreis K., Postma P.W., Lengeler J.W. Mol. Gen. Genet. 226:332-336(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 516-574. Strain: LT2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL19080.1. Sequence problems. X55456 Genomic DNA. Translation: CAA39101.1.
PIR	S15939.
RefSeq	NP_459121.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P40811.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM0116.
Proteomic databases
PaxDb	P40811.
PRIDE	P40811.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL19080; AAL19080; STM0116.
GeneID	1251634.
KEGG	stm:STM0116.
PATRIC	32378503. VBISalEnt20916_0121.
Phylogenomic databases
eggNOG	COG0028.
HOGENOM	HOG000258448.
KO	K01652.
OMA	MAMQNCD.
ProtClustDB	PRK07979.
Enzyme and pathway databases
UniPathway	UPA00047; UER00055. UPA00049; UER00059.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVI_SALTY

Accession

Primary (citable) accession number: P40811

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 19, 2001
Last modified:	May 1, 2013
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program