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Protein

Polyketide biosynthesis malonyl-ACP decarboxylase PksF

Gene

pksF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It decarboxylates selectively the malonyl group attached on the acyl-carrier-protein AcpK (Mal-AcpK).2 Publications

Catalytic activityi

A malonyl-[acyl-carrier protein] + H+ = an acetyl-[acyl-carrier protein] + CO2.1 Publication

Pathwayi: bacillaene biosynthesis

This protein is involved in the pathway bacillaene biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway bacillaene biosynthesis and in Antibiotic biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Lyase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU17140-MONOMER.
UniPathwayiUPA01003.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyketide biosynthesis malonyl-ACP decarboxylase PksF (EC:4.1.1.87)
Alternative name(s):
Malonyl acyl-carrier-protein decarboxylase
Gene namesi
Name:pksF
Ordered Locus Names:BSU17140
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001803501 – 415Polyketide biosynthesis malonyl-ACP decarboxylase PksFAdd BLAST415

Proteomic databases

PaxDbiP40804.
PRIDEiP40804.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009431.

Structurei

3D structure databases

ProteinModelPortaliP40804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Phylogenomic databases

eggNOGiENOG4108MH3. Bacteria.
COG0304. LUCA.
HOGENOMiHOG000060166.
InParanoidiP40804.
KOiK00646.
OMAiPGRQKDS.
PhylomeDBiP40804.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
SMARTiSM00825. PKS_KS. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.

Sequencei

Sequence statusi: Complete.

P40804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKCNLPEVV VTGVGVTASI GQGKEDFASS LLSGRHAFDV MKRSGRQKDS
60 70 80 90 100
RFIGAEIASL SYPDRLSKKM LRKASFSSRA ALVTLTEAWE EAELDDADSS
110 120 130 140 150
RIGLVVGGSN FQQRENFEVY ERYQDRSGFI SPAYGLSFMD SDLCGICTDQ
160 170 180 190 200
FGITGLAYTV GGASASGQLA VIHAIQQVLS GEVDTCIALG ALMDLSYMEC
210 220 230 240 250
EALRALGAMG TDKYADEPEN ACRPFDQNRD GFIYGESCGA LVIERKETAL
260 270 280 290 300
RRGLKPYAAL SGWSIKLDGN RNPDPSLEGE IHVIQKALER ARLLPEDIDY
310 320 330 340 350
INPHGTGSFI GDEIELKALR ACRLSHAYIN ATKSITGHGL SAAGIVEIIS
360 370 380 390 400
VLLQMKKSAL HPSRNLDHPI DDSFHWVNEK SISYRIKNAL SLSMGFGGMN
410
TAVCIQNIEK CGGES
Length:415
Mass (Da):45,009
Last modified:June 16, 2009 - v3
Checksum:iF7956D94DCEF7740
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168Q → QQ in AAA85139 (PubMed:7704258).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11039 Genomic DNA. Translation: AAA85139.1.
AL009126 Genomic DNA. Translation: CAB13585.2.
PIRiD69678.
RefSeqiNP_389594.2. NC_000964.3.
WP_003245287.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13585; CAB13585; BSU17140.
GeneIDi939500.
KEGGibsu:BSU17140.
PATRICi18975237. VBIBacSub10457_1810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11039 Genomic DNA. Translation: AAA85139.1.
AL009126 Genomic DNA. Translation: CAB13585.2.
PIRiD69678.
RefSeqiNP_389594.2. NC_000964.3.
WP_003245287.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP40804.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009431.

Proteomic databases

PaxDbiP40804.
PRIDEiP40804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13585; CAB13585; BSU17140.
GeneIDi939500.
KEGGibsu:BSU17140.
PATRICi18975237. VBIBacSub10457_1810.

Phylogenomic databases

eggNOGiENOG4108MH3. Bacteria.
COG0304. LUCA.
HOGENOMiHOG000060166.
InParanoidiP40804.
KOiK00646.
OMAiPGRQKDS.
PhylomeDBiP40804.

Enzyme and pathway databases

UniPathwayiUPA01003.
BioCyciBSUB:BSU17140-MONOMER.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
SMARTiSM00825. PKS_KS. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPKSF_BACSU
AccessioniPrimary (citable) accession number: P40804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 16, 2009
Last modified: October 5, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.