P40798 (STC_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein shuttle craft

Gene names

Name:	stc
ORF Names:	CG3647

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an essential role during the late stages of embryonic neurogenesis. May either fine-tune the guidance or the spatial maintenance of the migrating SNB and in nerve roots, which are composed of axons originating from distinct groups of motor neurons and may be required to either guide or maintain the position of these nerves along a direct and straight path to their ultimate targets in particular muscle fields. May play a role in egg chamber development and/or may confer essential maternal contributions to the early embryo.
Subcellular location	Nucleus.
Tissue specificity	Ovaries and embryonic central nervous system.
Developmental stage	Major expression is seen in the ovaries while moderate levels of expression are observed during embryogenesis and throughout subsequent stages of fly development.
Sequence similarities	Belongs to the NFX1 family. Contains 8 NF-X1-type zinc fingers. Contains 1 R3H domain. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding RNA-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from sequence or structural similarity Ref.2. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.1. Source: FlyBase
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P40798-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P40798-2)

The sequence of this isoform differs from the canonical sequence as follows:
     109-115: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1106	1106	Protein shuttle craft		PRO_0000056190
Regions
	Domain	1006 – 1071	66	R3H
	Zinc finger	386 – 433	48	RING-type; atypical
	Zinc finger	474 – 492	19	NF-X1-type 1
	Zinc finger	527 – 546	20	NF-X1-type 2
	Zinc finger	585 – 604	20	NF-X1-type 3
	Zinc finger	644 – 667	24	NF-X1-type 4
	Zinc finger	706 – 725	20	NF-X1-type 5
	Zinc finger	733 – 752	20	NF-X1-type 6
	Zinc finger	844 – 867	24	NF-X1-type 7
	Zinc finger	876 – 896	21	NF-X1-type 8
Amino acid modifications
	Modified residue	335	1	Phosphothreonine Ref.6
	Modified residue	336	1	Phosphoserine Ref.6
	Modified residue	339	1	Phosphoserine Ref.6
	Modified residue	343	1	Phosphoserine Ref.6
	Modified residue	354	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	109 – 115	7	Missing in isoform B.		VSP_005757
Experimental info
	Sequence conflict	12	1	A → P in AAB60255. Ref.1
	Sequence conflict	25	1	A → V in AAB60255. Ref.1
	Sequence conflict	617	1	M → I in AAB60255. Ref.1
	Sequence conflict	784	1	C → S in AAB60255. Ref.1
	Sequence conflict	820 – 821	2	EL → DW in AAB60255. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified February 2, 2004. Version 2. Checksum: 9502C6185994700D	FASTA	1,106	123,183
	10 20 30 40 50 60 MAEYWQQLTN GAGEAGPGNE SSAMADCNGG HESAAVGGSC NRHSNNNYVN FNQFIMQHNL 70 80 90 100 110 120 GGGAPSNATS TMQHPVGSSY TNFSLGGGGG AFGLNPPVAS ASTSHFANVS HQSPNFYSQA 130 140 150 160 170 180 MIPTYQNGDG IARVTVTSSY GSVNPSNSNF SSFYTPFGNN PFDFSASKLQ ASAPEFVPNF 190 200 210 220 230 240 AKLSLEETPA AATTNGNSTA SLETAINETR PRTLRAQEPA ERGANNQCSN HNYERERERE 250 260 270 280 290 300 RDRDRDRERD RDRDRDRDRD RDRDRDRDSR PGNTRQQRRS DYRDDREDRY ERSDRRRPQK 310 320 330 340 350 360 QQRYDNHRSN KRRDDWNRNR DRINGFPRAV DDLDTSNESA HPSPEKQSQL QQISPRRGPP 370 380 390 400 410 420 LPPADNEKLS QREKLVRDIE QRRLECLVCV EAIKSHQPTW SCRNCYHMLH LKCTITWASS 430 440 450 460 470 480 SKSEVGWRCP ACQNVLQDLP RDYLCFCGKL KNPPVSRTEL AHSCGEVCCR IEGCSHACTL 490 500 510 520 530 540 LCHPGPCPPC QANVVRSCGC GRSTKTMQCA MKEEVLCGEI CDKLLNCGEH RCQAECHSGK 550 560 570 580 590 600 CAACSEQVVQ QCHCGKQERK VPCTRESQDK RTYSCKDSCG QPLPCGHHKC KDSCHAGSCR 610 620 630 640 650 660 PCKLSPEQIT SCPCGKMPVP AGQRSSCLDP IPTCEGICSR TLRCGKPAHP HQCGSKCHLG 670 680 690 700 710 720 QCPPCPKQTG VKCRCGHMDQ MIKCRQLCNR ADDARCKRRC TKKRSCGKHK CNVECCIDID 730 740 750 760 770 780 HDCPLPCNRT LSCGKHKCDQ PCHRGNCPPC YRSSFEELYC ECGAEVIYPP VPCGTKKPIC 790 800 810 820 830 840 KLPCSRIHPC DHPPQHNCHS GPTCPPCMIF TTKLCHGNHE LRKTIPCSQP NFSCGMACGK 850 860 870 880 890 900 PLPCGGHKCI KPCHEGPCQS AGEICRQSCT KPRPTCGHKC AAACHEGACP ETPCKELVEV 910 920 930 940 950 960 QCECGNRKQN RSCQELAREH SRIATIQLAS SMAEMSRGNY MELSEILAPA KKSNKTLDCN 970 980 990 1000 1010 1020 DECRLLERNR RLAAALSSGN SDTKQKCLTK YSEFVRGFAK KNPALTKSVY ETLTDLVKLA 1030 1040 1050 1060 1070 1080 KESKQRSRSH SFPTMNREKR QLVHELCEVF GIESVSYDKE PNRNVVATAH KDRCWFPATS 1090 1100 IMEVLARESG QRRVPVPSNN AWGLKK
	Isoform B [UniParc]. Checksum: FC62621A04AB44BB	FASTA	1,099	122,433
	10 20 30 40 50 60 MAEYWQQLTN GAGEAGPGNE SSAMADCNGG HESAAVGGSC NRHSNNNYVN FNQFIMQHNL 70 80 90 100 110 120 GGGAPSNATS TMQHPVGSSY TNFSLGGGGG AFGLNPPVAS ASTSHFANFY SQAMIPTYQN 130 140 150 160 170 180 GDGIARVTVT SSYGSVNPSN SNFSSFYTPF GNNPFDFSAS KLQASAPEFV PNFAKLSLEE 190 200 210 220 230 240 TPAAATTNGN STASLETAIN ETRPRTLRAQ EPAERGANNQ CSNHNYERER ERERDRDRDR 250 260 270 280 290 300 ERDRDRDRDR DRDRDRDRDR DSRPGNTRQQ RRSDYRDDRE DRYERSDRRR PQKQQRYDNH 310 320 330 340 350 360 RSNKRRDDWN RNRDRINGFP RAVDDLDTSN ESAHPSPEKQ SQLQQISPRR GPPLPPADNE 370 380 390 400 410 420 KLSQREKLVR DIEQRRLECL VCVEAIKSHQ PTWSCRNCYH MLHLKCTITW ASSSKSEVGW 430 440 450 460 470 480 RCPACQNVLQ DLPRDYLCFC GKLKNPPVSR TELAHSCGEV CCRIEGCSHA CTLLCHPGPC 490 500 510 520 530 540 PPCQANVVRS CGCGRSTKTM QCAMKEEVLC GEICDKLLNC GEHRCQAECH SGKCAACSEQ 550 560 570 580 590 600 VVQQCHCGKQ ERKVPCTRES QDKRTYSCKD SCGQPLPCGH HKCKDSCHAG SCRPCKLSPE 610 620 630 640 650 660 QITSCPCGKM PVPAGQRSSC LDPIPTCEGI CSRTLRCGKP AHPHQCGSKC HLGQCPPCPK 670 680 690 700 710 720 QTGVKCRCGH MDQMIKCRQL CNRADDARCK RRCTKKRSCG KHKCNVECCI DIDHDCPLPC 730 740 750 760 770 780 NRTLSCGKHK CDQPCHRGNC PPCYRSSFEE LYCECGAEVI YPPVPCGTKK PICKLPCSRI 790 800 810 820 830 840 HPCDHPPQHN CHSGPTCPPC MIFTTKLCHG NHELRKTIPC SQPNFSCGMA CGKPLPCGGH 850 860 870 880 890 900 KCIKPCHEGP CQSAGEICRQ SCTKPRPTCG HKCAAACHEG ACPETPCKEL VEVQCECGNR 910 920 930 940 950 960 KQNRSCQELA REHSRIATIQ LASSMAEMSR GNYMELSEIL APAKKSNKTL DCNDECRLLE 970 980 990 1000 1010 1020 RNRRLAAALS SGNSDTKQKC LTKYSEFVRG FAKKNPALTK SVYETLTDLV KLAKESKQRS 1030 1040 1050 1060 1070 1080 RSHSFPTMNR EKRQLVHELC EVFGIESVSY DKEPNRNVVA TAHKDRCWFP ATSIMEVLAR 1090 ESGQRRVPVP SNNAWGLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A homolog of human transcription factor NF-X1 encoded by the Drosophila shuttle craft gene is required in the embryonic central nervous system." Stroumbakis N.D., Li Z., Tolias P.P. Mol. Cell. Biol. 16:192-201(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B). Tissue: Ovary.
[2]	"An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region." Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T., Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R. , Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M. Genetics 153:179-219(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila complementary DNA resource." Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M., Harvey D.A. Science 287:2222-2224(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-335; SER-336; SER-339; SER-343 AND SER-354, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09306 mRNA. Translation: AAB60255.1. AE014134 Genomic DNA. Translation: AAF53441.2. AE014134 Genomic DNA. Translation: AAN10891.1. AF145679 mRNA. Translation: AAD38654.1.
PIR	T13938. T44598.
RefSeq	NP_476598.1. NM_057250.4. NP_476599.1. NM_057251.4.
UniGene	Dm.7818.
3D structure databases
ProteinModelPortal	P40798.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40798. 2 interactions.
MINT	MINT-279983.
Proteomic databases
PaxDb	P40798.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0080705; FBpp0080265; FBgn0001978.
GeneID	34888.
KEGG	dme:Dmel_CG3647.
Organism-specific databases
CTD	34888.
FlyBase	FBgn0001978. stc.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00400000022083.
InParanoid	P40798.
KO	K12236.
OMA	LVRYCPC.
OrthoDB	EOG47D7X1.
PhylomeDB	P40798.
Gene expression databases
Bgee	P40798.
GermOnline	CG3647. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001374. R3H_ss-bd. IPR019786. Zinc_finger_PHD-type_CS. IPR000967. Znf_NFX1. IPR001841. Znf_RING. [Graphical view]
Pfam	PF01424. R3H. 1 hit. PF01422. zf-NF-X1. 2 hits. [Graphical view]
SMART	SM00393. R3H. 1 hit. SM00184. RING. 1 hit. SM00438. ZnF_NFX. 9 hits. [Graphical view]
PROSITE	PS51061. R3H. 1 hit. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	34888.
NextBio	790717.

Entry information

Entry name

STC_DROME

Accession

Primary (citable) accession number: P40798
Secondary accession number(s): Q8IP49, Q9VJQ1, Q9Y0Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 2, 2004
Last modified:	May 1, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project