Reviewed, UniProtKB/Swiss-Prot P40797 (PNUT_DROME)

Names and origin

Protein names

Recommended name:
    Protein peanut

Gene names

Name:	pnut
ORF Names:	CG8705

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cytokinesis and possibly cellularization. Also acts as an enhancer of the sina gene, thus having a role in photoreceptor development. Ref.1
Subunit structure	May well assemble into a multicomponent structure.
Subcellular location	Note: Localized to the cleavage furrow of dividing cells during cytokinesis and to the intercellular bridge connecting postmitotic daughter cells. Equally found on the cell surfaces of the embryonic central nervous system and on the apical membranes of developing photoreceptor cells in the eye imaginal disk. Ref.1
Developmental stage	Maternal gene products found in the early embryo prior to zygotic transcription. Ref.1
Sequence similarities	Belongs to the septin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	Coiled coil
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from genetic interaction. Source: FlyBase cellularization Traceable author statement. Source: FlyBase cytokinesis, contractile ring formation Non-traceable author statement. Source: FlyBase positive regulation of apoptosis Inferred from direct assay. Source: FlyBase vesicle docking during exocytosis Traceable author statement. Source: FlyBase vesicle targeting Traceable author statement. Source: FlyBase
Cellular component	germline ring canal Non-traceable author statement. Source: FlyBase
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW actin binding Inferred from direct assay. Source: FlyBase microtubule binding Inferred from direct assay. Source: FlyBase ubiquitin protein ligase binding Inferred from physical interaction. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VHB1	1	EBI-171301,EBI-89809

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Protein peanut		PRO_0000173510
Regions
	Nucleotide binding	149 – 156	8	GTP By similarity
	Nucleotide binding	290 – 296	7	GTP By similarity
	Coiled coil	420 – 516	97	Potential
Sites
	Binding site	345	1	GTP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	362	1	GTP By similarity
Amino acid modifications
	Modified residue	6	1	Phosphoserine Ref.5
	Modified residue	13	1	Phosphoserine Ref.5
	Modified residue	517	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	79	1	G → S in AAA19603. Ref.1
	Sequence conflict	249	1	S → N in AAA19603. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40797-1 [UniParc]. Last modified November 8, 2005. Version 2. Checksum: A8009F7E6F331A32	FASTA	539	60,143
	10 20 30 40 50 60 MNSPRSNAVN GGSGGAISAL PSTLAQLALR DKQQAASASA SSATNGSSGS ESLVGVGGRP 70 80 90 100 110 120 PNQPPSVPVA ASGKLDTSGG GASNGDSNKL THDLQEKEHQ QAQKPQKPPL PVRQKPMEIA 130 140 150 160 170 180 GYVGFANLPN QVYRKAVKRG FEFTLMVVGA SGLGKSTLIN SMFLSDIYNA EQYPGPSLRK 190 200 210 220 230 240 KKTVAVEATK VMLKENGVNL TLTVVDTPGF GDAVDNSNCW VPILEYVDSK YEEYLTAESR 250 260 270 280 290 300 VYRKTISDSR VHCCLYFIAP SGHGLLPLDI ACMQSLSDKV NLVPVIAKAD TMTPDEVHLF 310 320 330 340 350 360 KKQILNEIAQ HKIKIYDFPA TLEDAAEEAK TTQNLRSRVP FAVVGANTII EQDGKKVRGR 370 380 390 400 410 420 RYPWGLVEVE NLTHCDFIAL RNMVIRTHLQ DLKDVTNNVH YENYRCRKLS ELGLVDGKAR 430 440 450 460 470 480 LSNKNPLTQM EEEKREHEQK MKKMEAEMEQ VFDMKVKEKM QKLRDSELEL ARRHEERKKA 490 500 510 520 530 LELQIRELEE KRREFEREKK EWEDVNHVTL EELKRRSLGA NSSTDNVDGK KEKKKKGLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins." Neufeld T.P., Rubin G.M. Cell 77:371-379(1994) [PubMed: 8181057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6 AND SER-13, MASS SPECTROMETRY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U08103 mRNA. Translation: AAA19603.1. AE013599 Genomic DNA. Translation: AAM68857.1. AY058663 mRNA. Translation: AAL13892.1.
PIR	A54294.
RefSeq	NP_477064.1. NP_724659.1.
UniGene	Dm.4132
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P40797. 8 interactions.
Genome annotation databases
Ensembl	FBgn0013726. Drosophila melanogaster. [Contig view]
GeneID	35801.
KEGG	dme:Dmel_CG8705.
Organism-specific databases
FlyBase	FBgn0013726. pnut.
Phylogenomic databases
HOGENOM	P40797.
OMA	P40797. INSMFLS.
Gene expression databases
ArrayExpress	P40797.
GermOnline	CG8705. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000038. Cell_Div_GTP_bd. [Graphical view]
PANTHER	PTHR18884. Cell_Div_GTP_bd. 1 hit.
Pfam	PF00735. Septin. 1 hit. [Graphical view]
ProDom	PD002565. GTP_Cell_Div. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	795276.

Entry information

Entry name

PNUT_DROME

Accession

Primary (citable) accession number: P40797
Secondary accession number(s): Q0E9F5, Q9V385

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project