Reviewed, UniProtKB/Swiss-Prot P40794 (BNK_DROME)

Names and origin

Protein names

Recommended name:
    Protein bottleneck

Gene names

Name:	bnk
ORF Names:	CG1480

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts as a regulator of the microfilament network governing cellularization of the embryo. Determines the timing of a key conformational transition in the cortical microfilament network: the proper coordination of membrane invagination and basal closure of the cells. To do this, bnk possibly physically links neighboring contractile units of the early cycle 14 microfilament network in a manner that prevents basal constriction until the proper stage has been reached. Bnk together with nullo and Sry-alpha may provide auxiliary functions, by acting both to stabilize a large and dynamic microfilament structure and regulate its functions.
Subcellular location	Cytoplasm › cytoskeleton. Note: Colocalizes with the structural transitions in the microfilament network during cellularization. Association is observed only when the network assumes an aligned and tightly apposed hexagonal configuration.
Tissue specificity	Restricted to the blastoderm.
Developmental stage	Observed exclusively in the late synctial and cellular blastoderm stages of development. Transcripts are first detected during nuclear division cycle 11. They reach a strong peak at cycle 14 and from then on gradually decline until they are no longer detectable at the end of cellularization.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin filament organization Traceable author statement. Source: FlyBase cellularization Traceable author statement. Source: FlyBase cytokinesis, contractile ring formation Non-traceable author statement. Source: FlyBase membrane organization Non-traceable author statement. Source: FlyBase
Cellular component	actin filament Ref.1 Inferred from direct assay. Source: FlyBase cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Protein bottleneck		PRO_0000064966
Regions
	Compositional bias	108 – 137	30	Gln-rich
Experimental info
	Sequence conflict	115	1	R → Q in AAC46467. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40794-1 [UniParc]. Last modified October 18, 2001. Version 2. Checksum: 46E337ADC566C23B	FASTA	303	33,566
	10 20 30 40 50 60 MSISTFNFQF YNYKLSPSSP GFGSTASRSS SSCISELEMD IDEDMSAQPT ITSTPKPRFT 70 80 90 100 110 120 SQLAVELAKT EPGNGISPLR PKLHTAQKRW SMELREKVLE MSKRNNGQEK PQTARQQEQR 130 140 150 160 170 180 QPQEQPLQQE ELQHQQQEPT VTDKINFFNK LTNTFESGFS KLMPQASSTN NRFIAMLRTA 190 200 210 220 230 240 RPQNVATTTA NSSTANSFLG SDHSLSGSVT GQVPPPKPKR LSATTAQFAT PHVPAMGVGK 250 260 270 280 290 300 GGSQRKCSLR RKPSMDKSRA TISRQSSSAS VRTQNHAIME DLSLVVPVRL RIAEYEQRIS MSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bottleneck acts as a regulator of the microfilament network governing cellularization of the Drosophila embryo." Schejter E.D., Wieschaus E. Cell 75:373-385(1993) [PubMed: 8402919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U01035 mRNA. Translation: AAC46467.1. AE014297 Genomic DNA. Translation: AAF57125.1.
PIR	A49072.
RefSeq	NP_524604.2.
UniGene	Dm.1899
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0004389. Drosophila melanogaster. [Contig view]
GeneID	43687.
KEGG	dme:Dmel_CG1480.
NMPDR	fig|7227.3.peg.15556.
Organism-specific databases
FlyBase	FBgn0004389. bnk.
Phylogenomic databases
HOGENOM	P40794.
OMA	P40794. ELEMDID.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014281-MON.
Gene expression databases
ArrayExpress	P40794.
GermOnline	CG1480. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	835248.

Entry information

Entry name

BNK_DROME

Accession

Primary (citable) accession number: P40794
Secondary accession number(s): Q9VA03

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 18, 2001
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project