Reviewed, UniProtKB/Swiss-Prot P40792 (RAC1_DROME)

Names and origin

Protein names

Recommended name:
    Ras-related protein Rac1

Gene names

Name:	Rac1
Synonyms:	RacA
ORF Names:	CG2248

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in axon outgrowth and myoblast fusion. Ref.1
Subunit structure	Interacts with Sra-1. Ref.7
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	JNK cascade Inferred from mutant phenotype. Source: FlyBase actin cytoskeleton organization Inferred from mutant phenotype. Source: FlyBase adherens junction maintenance Inferred from mutant phenotype. Source: FlyBase axon extension Inferred from mutant phenotype. Source: FlyBase axon guidance Ref.7 Inferred from mutant phenotype. Source: FlyBase axonal fasciculation Inferred from mutant phenotype. Source: FlyBase border follicle cell migration Inferred from mutant phenotype. Source: FlyBase cell competition in a multicellular organism Inferred from mutant phenotype. Source: FlyBase dendrite morphogenesis Traceable author statement. Source: FlyBase dorsal appendage formation Inferred from mutant phenotype. Source: FlyBase dorsal closure, amnioserosa morphology change Inferred from mutant phenotype. Source: FlyBase dorsal closure, elongation of leading edge cells Inferred from mutant phenotype. Source: FlyBase establishment of ommatidial polarity Non-traceable author statement. Source: FlyBase germ-band shortening Inferred from mutant phenotype. Source: FlyBase glial cell migration Inferred from mutant phenotype. Source: FlyBase head involution Inferred from mutant phenotype. Source: FlyBase hemocyte development Inferred from mutant phenotype. Source: FlyBase hemocyte migration Inferred from mutant phenotype. Source: FlyBase imaginal disc-derived wing hair site selection Inferred from mutant phenotype. Source: FlyBase lamellipodium assembly Inferred from mutant phenotype. Source: FlyBase microtubule-based process Non-traceable author statement. Source: FlyBase morphogenesis of larval imaginal disc epithelium Traceable author statement. Source: FlyBase myoblast fusion Inferred from mutant phenotype. Source: FlyBase myoblast proliferation Inferred from mutant phenotype. Source: FlyBase peripheral nervous system development Inferred from mutant phenotype. Source: FlyBase phagocytosis triggered by activation of immune response cell surface activating receptor Inferred from direct assay. Source: FlyBase phagocytosis, engulfment Inferred from mutant phenotype. Source: FlyBase regulation of axonogenesis Inferred from genetic interaction. Source: FlyBase regulation of dendrite morphogenesis Inferred from genetic interaction. Source: FlyBase regulation of hemocyte differentiation Inferred from mutant phenotype. Source: FlyBase regulation of synapse organization Ref.7 Inferred from genetic interaction. Source: FlyBase rhabdomere development Inferred from genetic interaction. Source: FlyBase salivary gland morphogenesis Inferred from mutant phenotype. Source: FlyBase small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro tracheal outgrowth, open tracheal system Inferred from genetic interaction. Source: FlyBase ventral cord development Inferred from mutant phenotype. Source: FlyBase
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell rhabdomere Inferred from direct assay. Source: FlyBase
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: FlyBase protein binding Ref.7 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pak	Q24213	2	EBI-74845,EBI-74826
Rac2	P48554	1	EBI-74845,EBI-74869

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Ras-related protein Rac1		PRO_0000198893
	Propeptide	190 – 192	3	Removed in mature form By similarity		PRO_0000281244
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	115 – 118	4	GTP By similarity
	Motif	32 – 40	9	Effector region Potential
Amino acid modifications
	Modified residue	189	1	Cysteine methyl ester By similarity
	Lipidation	189	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Sequence conflict	135	1	A → V in AAA62870. Ref.1
	Sequence conflict	143 – 146	4	LAMA → SGHG in AAA67040. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P40792-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: F910B54BDBD8AFA3	FASTA	192	21,354
	10 20 30 40 50 60 MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDAKP INLGLWDTAG 70 80 90 100 110 120 QEDYDRLRPL SYPQTDVFLI CFSLVNPASF ENVRAKWYPE VRHHCPSTPI ILVGTKLDLR 130 140 150 160 170 180 DDKNTIEKLR DKKLAPITYP QGLAMAKEIG AVKYLECSAL TQKGLKTVFD EAIRSVLCPV 190 LQPKSKRKCA LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distinct morphogenetic functions of similar small GTPases: Drosophila Drac1 is involved in axonal outgrowth and myoblast fusion." Luo L., Liao Y.J., Jan L.Y., Jan Y. Genes Dev. 8:1787-1802(1994) [PubMed: 7958857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: Oregon-R.
[2]	"Characterization of rho GTPase family homologues in Drosophila melanogaster: overexpressing Rho1 in retinal cells causes a late developmental defect." Hariharan I.K., Hu K.-Q., Asha H., Quintanilla A., Ezzell R.M., Settleman J. EMBO J. 14:292-302(1995) [PubMed: 7835340] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A dominant inhibitory version of the small GTP-binding protein Rac disrupts cytoskeletal structures and inhibits developmental cell shape changes in Drosophila." Harden N., Loh H., Chia W., Lim L. Development 121:903-914(1995) [PubMed: 7720592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Canton-S.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[7]	"CYFIP/Sra-1 controls neuronal connectivity in Drosophila and links the Rac1 GTPase pathway to the fragile X protein." Schenck A., Bardoni B., Langmann C., Harden N., Mandel J.-L., Giangrande A. Neuron 38:887-898(2003) [PubMed: 12818175] [Abstract] Cited for: INTERACTION WITH SRA-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U11823 mRNA. Translation: AAA62870.1. L38309 mRNA. Translation: AAA67040.1. Z35642 mRNA. Translation: CAA84709.1. AE014296 Genomic DNA. Translation: AAF47469.1. AY060408 mRNA. Translation: AAL25447.1.
PIR	I45715. S51718. S54295.
RefSeq	NP_476950.1.
UniGene	Dm.4299
3D structure databases
HSSP	HSSP built from PDB template 1G4U based on UniProtKB P15154.
SMR	P40792. Positions 1-177.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40792. 34 interactions.
Genome annotation databases
Ensembl	FBgn0010333. Drosophila melanogaster. [Contig view]
GeneID	38146.
KEGG	dme:Dmel_CG2248.
NMPDR	fig|7227.3.peg.7516.
Organism-specific databases
FlyBase	FBgn0010333. Rac1.
Phylogenomic databases
HOGENOM	P40792.
OMA	P40792. TQVGVKE.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014604-MON.
Gene expression databases
ArrayExpress	P40792.
GermOnline	CG2248. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003578. GTPase_Rho. IPR013753. Ras. IPR001806. Ras_GTPase. IPR005225. Small_GTP_bd. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	807185.

Entry information

Entry name

RAC1_DROME

Accession

Primary (citable) accession number: P40792
Secondary accession number(s): Q9W0H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project