Reviewed, UniProtKB/Swiss-Prot P40757 (ALN_RANCA)

Names and origin

Protein names

Recommended name:
    Allantoinase, mitochondrial
    EC=3.5.2.5

Gene names

Name:

ALN

Organism

Rana catesbeiana (Bull frog)

Taxonomic identifier

8400 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Raninae › Rana › Aquarana

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-allantoin + H2O = allantoate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion.
Tissue specificity	Liver and kidney.
Post-translational modification	Carbamylation allows a single lysine to coordinate two zinc ions By similarity.
Sequence similarities	Belongs to the DHOase family. Allantoinase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	allantoin catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	allantoinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 484		Allantoinase, mitochondrial		PRO_0000007371
Sites
	Metal binding	76	1	Zinc 1 By similarity
	Metal binding	78	1	Zinc 1 By similarity
	Metal binding	163	1	Zinc 1; via carbamate group By similarity
	Metal binding	163	1	Zinc 2; via carbamate group By similarity
	Metal binding	199	1	Zinc 2 By similarity
	Metal binding	251	1	Zinc 2 By similarity
	Metal binding	324	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	163	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40757-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A2DC377B850DC402	FASTA	484	53,257
	10 20 30 40 50 60 MALKSKPGIM NITPGSKISV IRSKRVIQAN TISSCDIIIS DGKISSVLAW GKHVTSGAKL 70 80 90 100 110 120 LDVGDLVVMA GIIDPHVHVN EPGRTDWEGY RTATLAAAAG GITAIVDMPL NSLPPTTSVT 130 140 150 160 170 180 NFHTKLQAAK RQCYVDVAFW GGVIPDNQVE LIPMLQAGVA GFKCFLINSG VPEFPHVSVT 190 200 210 220 230 240 DLHTAMSELQ GTNSVLLFHA ELEIAKPAPE IGDSTLYQTF LDSRPDDMEI AAVQLVADLC 250 260 270 280 290 300 QQYKVRCHIV HLSSAQSLTI IRKAKEAGAP LTVETTHHYL SLSSEHIPPG ATYFKCCPPV 310 320 330 340 350 360 RGHRNKEALW NALLQGHIDM VVSDHSPCTP DLKLLKEGDY MKAWGGISSL QFGLPLFWTS 370 380 390 400 410 420 ARTRGFSLTD VSQLLSSNTA KLCGLGIVKE PLKWVMMLIW SSGILTKSFR CKKMIFITRI 430 440 450 460 470 480 SSPHIWDSFF KEKSWLLLFE GLLFISKGSM LPNQLENLFL YTLWSLVKPV HPVHPIIRKN LPHI

References

[1]

"Amphibian allantoinase. Molecular cloning, tissue distribution, and functional expression." Hayashi S., Jain S., Chu R., Alvares K., Xu B., Erfurth F., Usuda N., Rao M.S., Reddy S.K., Noguchi T., Reddy J.K., Yeldandi A.Y. J. Biol. Chem. 269:12269-12276(1994) [PubMed: 8163532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 365-381. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03471 mRNA. Translation: AAA19116.1.
PIR	A53595.
3D structure databases
SMR	P40757. Positions 20-401.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P40757.
Enzyme and pathway databases
BRENDA	3.5.2.5. 829.
Family and domain databases
InterPro	IPR017593. Allantoinase. IPR006680. Amidohydro_1. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR03178. allantoinase. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALN_RANCA

Accession

Primary (citable) accession number: P40757

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)